Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicin...Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicinal properties.Galloylation and glycosylation are key steps in the formation of polyphenolic compounds,as the glycosylation of flavonoids is required for the acylation of flavonoid glycosides,and the glycosylation of gallic acid is necessary for its role as an acyl donor.Therefore,glycosylation to generate the acyl donor or acceptor is a core step in the biosynthesis of polyphenolic compounds.However,how this glycosylation occurs in P.chinense is unknown.In this study,we determined that the UDP-glucose transferase PcUGT84A82 mediates the glycosylation of gallic acid and pinocembrin to produce 1-O-Galloyl-β-D-glucose and pinocembroside,respectively.Metabolic profiling of polyphenolic compounds using UHPLC-ESI–Q-TOF/MS revealed high levels of polyphenols in flowers,leaves,and roots,and low levels in stems of P.chinense.We performed isoform-sequencing(Iso-seq)to assemble a full-length transcriptome of P.chinense,from which we identified 58 UGT family members.PcUGT84A82 is highly similar to functional UGTs in other plant species,and PcUGT84A82 transcript levels were positively correlated with the levels of various polyphenolic compounds.We validated the function of PcUGT84A82 via in vitro enzyme assays and transient expression in Nicotiana benthamiana leaves.Subcellular localization tests showed that PcUGT84A82 localizes to the nucleus and cytoplasm.In summary,PcUGT84A82 catalyzes the conversion of gallic acid to 1-O-Galloyl-β-D-glucose as the acyl donor and pinocembrin to pinocembroside as the acyl acceptor,mediating the biosynthesis of galloylated macrocyclic polyphenolic compounds in P.chinense.These findings lay the foundation for elucidating the entire biosynthetic pathway of active polyphenols in this important herbal plant species.展开更多
Background:Aspiration pneumonia is a severe health concern,particularly for ICU patients with impaired airway defenses.Current animal models fail to fully replicate the condition,focusing solely on chemical lung injur...Background:Aspiration pneumonia is a severe health concern,particularly for ICU patients with impaired airway defenses.Current animal models fail to fully replicate the condition,focusing solely on chemical lung injury from gastric acid while neglecting pathogen-induced inflammation.This gap hinders research on pathogenesis and treatment,creating an urgent need for a clinically relevant model.This study aimed to develop an improved rat model of aspiration pneumonia by combining hydrochloric acid(HCl)and lipopolysaccharide(LPS)administration.Methods:Specific pathogen-free Sprague Dawley rats underwent intratracheal instillation of HCl and LPS.Techniques included rat weight measurement,tracheal intubation,pulmonary function monitoring,lung tissue sampling with HE staining and scoring,bronchoalveolar lavage fluid(BALF)sampling,protein and inflammatory cytokine analysis via BCA and ELISA,BALF pH determination,Evans Blue dye assessment,blood gas analysis,FITC-dextran leakage,Western blotting,electron microscopy,survival analysis,and transcriptome sequencing with bioinformatics.Statistical analysis was performed using GraphPad Prism.Results:The optimal model involved instillation of 1.5μL/g.wt HCl(pH=1)followed by 20μg/g.wt LPS after 1 h.This model reproduced acute lung injury,including tissue damage,pulmonary microvascular dysfunction,inflammatory responses,hypoxemia,and impaired pulmonary ventilation,with recovery observed at 72 h.PANoptosis was confirmed,characterized by increased markers.Concentration-dependent effects of HCl and LPS on lung damage were identified,alongside cytokine elevation and microvascular dysfunction.Conclusions:This optimized model closely mimics clinical aspiration pneumonia,providing a valuable tool for studying pathophysiology and therapeutic strategies.展开更多
High-quality entangling gates are crucial for scalable quantum information processing.Implementing all-microwave two-qubit gates on fixed-frequency transmons offers advantages in reducing wiring complexity,but the gat...High-quality entangling gates are crucial for scalable quantum information processing.Implementing all-microwave two-qubit gates on fixed-frequency transmons offers advantages in reducing wiring complexity,but the gate performance is often limited due to the residual ZZ interaction and the frequency crowding problem.Here,we introduce a novel scheme that enables a microwave drive-activated CZ gate compatible with the coupler structure to suppress the residual ZZ interaction.The microwave drive is applied to the coupler and the microwave drive frequency remains far detuned from the system’s transition frequency to alleviate the frequency crowding problem.We model the gate process analytically and demonstrate a theoretical gate fidelity up to 99.9%numerically.Our scheme is compatible with current coupler-structure-based circuits,and insensitive to microwave crosstalk,showing a possible path for all-microwave quantum operations at scale.展开更多
The construction of scintillating ceramics is of great technological importance for various fundamental applications, including medical diagnostic, security inspection, resource exploration and particle physics. The c...The construction of scintillating ceramics is of great technological importance for various fundamental applications, including medical diagnostic, security inspection, resource exploration and particle physics. The chief challenge is the facile and scalable synthesis of scintillating ceramics with the desirable combination of pore-free, reliable mechanical properties and excellent scintillating performance. Here we present a pressureless glass crystallization strategy for the construction of scintillating composite with high crystallinity. The fabricated scintillating composites are featured by small optical turbidity, excellent mechanical properties, and efficient scintillating luminescence with the scintillating light yield of 15,000 pH/MeV and about 2.46 times higher than that of the commercial BGO single crystal. Moreover, the scintillating composite derived radiation detector device is successfully elaborated. The practical application for monitoring gamma ray is demonstrated and the precision of the device is less than that of the tolerable deviation of 30%. Our results suggest an innovative approach for expanding the category of scintillating material candidates, pointing to practical application in the field of radiation detection.展开更多
Heterostructured(HS)material with extraordinary mechanical properties has been regarded as one of the most promising structural materials.Here,we reported a new strategy for preparing heterostructured pure titanium la...Heterostructured(HS)material with extraordinary mechanical properties has been regarded as one of the most promising structural materials.Here,we reported a new strategy for preparing heterostructured pure titanium laminates that possess a good combination of strength and ductility by combining gradient structure(GS)and heterogeneous lamella structure(HLS).The deformation characteristic versus microstructure evolution of GS/HLS titanium laminates,namely the strain partitions between different-sized grains(480–25μm)was visualized using a scanning electron microscope(SEM)equipped with electron backscattered diffraction(EBSD)mode combined with the digital image correlation(SEM-DIC)with an ultrahigh spatial resolution for the first time.As a result,the hetero-deformation of unique GS/HLS structure by the characteristic of strain partitions could be accurately captured.While the hetero-deformation could result in the hetero-deformation induced(HDI)stress strengthening and HDI hardening,which were regarded as the key reason that the resulting GS/HLS Ti laminates showed a superior combination of strength and ductility.This could promote a more in-depth understanding of the strengtheningtoughening mechanism of heterostructured material.展开更多
The discovery of high-temperature superconductivity in La_(3)Ni_(2)O_(7) and La_(4)Ni_(3)O_(10) under pressure has garnered extensive attention.Herein,we report systematic investigations on the structure,magnetism,and...The discovery of high-temperature superconductivity in La_(3)Ni_(2)O_(7) and La_(4)Ni_(3)O_(10) under pressure has garnered extensive attention.Herein,we report systematic investigations on the structure,magnetism,and electrical resistance evolutions of Pr_(4)Ni_(3)O_(10−δ)polycrystalline samples under various pressures.Pr_(4)Ni_(3)O_(10−δ)exhibits density wave transitions on Ni and Pr sublattices at approximately 157.6 and 4.3 K,respectively.The density wave can be progressively suppressed by pressure.A structural transformation from the monoclinic P21/a space group to the tetragonal I4/mmm occurs around 20GPa.An apparent drop in resistance with an evident magnetic field dependence is observed at pressures above 20GPa,indicating the emergence of superconductivity.The discovery of superconductivity in Pr_(4)Ni_(3)O_(10−δ)broadens the family of nickelate superconductors.Pr_(4)Ni_(3)O_(10−δ)provides a new platform for investigation of the mechanisms of superconductivity in the Ruddlesden-Popper phases of nickelates.展开更多
Algicidal bacteria have been frequently isolated from algal blooming areas.However,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal b...Algicidal bacteria have been frequently isolated from algal blooming areas.However,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is limited.In this study,we investigated the composition of the microbial community coexisting with harmful alga Karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in microcosms.The microorganisms inhabiting the K.mikimotoi culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal cultivation.Both the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of K.mikimotoi and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas increased.Core operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the K.mikimotoi culture with or without treatments.Collectively,this study indicated that microbial community inhabiting K.mikimotoi culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of K.mikimotoi via nutrient stimulation or by adding isolated algicidal strains.展开更多
To support semantic inter-operability between the biomedical information systems, it is necessary to determine the correspondences between the heterogeneous biomedical concepts, which is commonly known as biomedical o...To support semantic inter-operability between the biomedical information systems, it is necessary to determine the correspondences between the heterogeneous biomedical concepts, which is commonly known as biomedical ontology matching. Biomedical concepts are usually complex and ambiguous, which makes matching biomedical ontologies a challenge. Since none of the similarity measures can distinguish the heterogeneous biomedical concepts in any context independently, usually several similarity measures are applied together to determine the biomedical concepts mappings. However, the ignorance of the effects brought about by different biomedical concept mapping’s preference on the similarity measures significantly reduces the alignment’s quality. In this study, a non-dominated sorting genetic algorithm (NSGA)-III-based biomedical ontology matching technique is proposed to effectively match the biomedical ontologies, which first utilises an ontology partitioning technique to transform the large-scale biomedical ontology matching problem into several ontology segment-matching problems, and then uses NSGA-III to determine the optimal alignment without tuning the aggregating weights. The experiment is conducted on the anatomy track and large biomedic ontologies track which are provided by the Ontology Alignment Evaluation Initiative (OAEI), and the comparisons with OAEI’s participants show the effectiveness of the authors’ approach.展开更多
A kind of dielectric barrier discharge（DBD） device composed of water electrodes with 3×3forms can produce large-area low-temperature plasmas at atmospheric pressure.To reflect the discharge characteristics of D...A kind of dielectric barrier discharge（DBD） device composed of water electrodes with 3×3forms can produce large-area low-temperature plasmas at atmospheric pressure.To reflect the discharge characteristics of DBD better,a dynamic simulation model,which is based on the voltage controlled current source（CCS）,is established,then the established model in Matlab/Simulink is used to simulate the DBD in air.The voltage-current waves and Lissajous at a voltage of 10 kV,11 kV and 12 kV peak value with a frequency of 15 kHz are studied.The change of the discharge power of DBD with a different amplitude and frequency of applied voltage is also analyzed.The result shows the voltage-current waves,Lissajous and discharge power of DBD under different conditions from the simulation agree well with those of the experiment.In addition,we propose a method to calculate the dielectric barrier capacitance Cd and the gap capacitance Cg,which is valid through analyzing the variation of capacitance at different voltage amplitudes.展开更多
Plants have mechanisms to transport secondary metabolites from where they are biosynthesized to the sites where they function,or to sites such as the vacuole for detoxification.However,current research has mainly focu...Plants have mechanisms to transport secondary metabolites from where they are biosynthesized to the sites where they function,or to sites such as the vacuole for detoxification.However,current research has mainly focused on metabolite biosynthesis and regulation,and little is known about their transport.Tanshinone,a class diterpenoid with medicinal properties,is biosynthesized in the periderm of Salvia miltiorrhiza roots.Here,we discovered that tanshinone can be transported out of peridermal cells and secreted into the soil environment and that the ABC transporter SmABCG1 is involved in the efflux of tanshinoneⅡA and tanshinoneⅠ.The SmABCG1 gene is adjacent to the diterpene biosynthesis gene cluster in the S.miltiorrhiza genome.The temporal–spatial expression pattern of SmABCG1 is consistent with tanshinone accumulation profiles.SmABCG1 is located on the plasma membrane and preferentially accumulates in the peridermal cells of S.miltiorrhiza roots.Heterologous expression in Xenopus laevis oocytes demonstrated that SmABCG1 can export tanshinoneⅡA and tanshinoneⅠ.CRISPR/Cas9-mediated mutagenesis of SmABCG1 in S.miltiorrhiza hairy roots resulted in a significant decrease in tanshinone contents in both hairy roots and the culture medium,whereas overexpression of this gene resulted in increased tanshinone contents.CYP76AH3 transcript levels increased in hairy roots overexpressing SmABCG1 and decreased in knockout lines,suggesting that SmABCG1 may affect the expression of CYP76AH3,indirectly regulating tanshinone biosynthesis.Finally,tanshinoneⅡA showed cytotoxicity to Arabidopsis roots.These findings offer new perspectives on plant diterpenoid transport and provide a new genetic tool for metabolic engineering and synthetic biology research.展开更多
Flavonoids,the largest class of polyphenols,exhibit substantial structural and functional diversity,yet their evolutionary diversification and specialized functions remain largely unexplored.The genus Scutellaria is n...Flavonoids,the largest class of polyphenols,exhibit substantial structural and functional diversity,yet their evolutionary diversification and specialized functions remain largely unexplored.The genus Scutellaria is notable for its rich flavonoid diversity,particularly of 6/8-hydroxylated variants biosynthesized by the cytochrome P450 subfamily CYP82D.Our study analyzes metabolic differences between Scutellaria baicalensis and Scutellaria barbata,and the results suggest that CYP82Ds have acquired a broad range of catalytic functions over their evolution.By integrating analyses of metabolic networks and gene evolution across 22 Scutellaria species,we rapidly identified 261 flavonoids and delineated five clades of CYP82Ds associated with various catalytic functions.This approach revealed a unique catalytic mode for 6/8-hydroxylation of flavanone substrates and the first instance of 7-O-demethylation of flavonoid substrates catalyzed by a cytochrome P450.Ancestral sequence reconstruction and functional validation demonstrated that gradual neofunctionalization of CYP82Ds has driven the chemical diversity of flavonoids in the genus Scutellaria throughout its evolutionary history.These findings enhance our understanding of flavonoid diversity,reveal the intricate roles of CYP82Ds in Scutellaria species,and highlight the extensive catalytic versatility of cytochrome P450 members within plant taxa.展开更多
Data-driven process monitoring methods are widely used in industrial tasks,with visual monitoring enabling operators to intuitively understand operational status,which is vital for maximizing industrial safety and pro...Data-driven process monitoring methods are widely used in industrial tasks,with visual monitoring enabling operators to intuitively understand operational status,which is vital for maximizing industrial safety and production efficiency.However,high-dimensional industrial data often exhibit complex structures,making the traditional 2D visualization methods ineffective at distinguishing different fault types.Thus,a visual process monitoring method that combines supervised uniform manifold approximation and projection with a label assignment strategy is proposed herein.First,the proposed supervised projection method enhances the visualization step by incorporating label information to guide the nonlinear dimensionality reduction process,improving the degrees of class separation and intraclass compactness.Then,to address the lack of label information for online samples,a label assignment strategy is designed.This strategy integrates kernel Fisher discriminant analysis and Bayesian inference,assigning different label types to online samples based on their confidence levels.Finally,upon integrating the label assignment strategy with the proposed supervised projection method,the assigned labels enhance the separability of online projections and enable the visualization of unknown data to some extent.The proposed method is validated on the Tennessee Eastman process and a real continuous catalytic reforming process,demonstrating superior visual fault monitoring and diagnosis performance to that of the state-of-the-art methods,especially in real industrial applications.展开更多
Trichomes, small protrusions on the surface of many plant species, can produce and store various secondary metabolic products. Artemisinin, the most famous and potent medicine for malaria, is synthesized, stored, and ...Trichomes, small protrusions on the surface of many plant species, can produce and store various secondary metabolic products. Artemisinin, the most famous and potent medicine for malaria, is synthesized, stored, and secreted by Artemisia annua trichomes. However, the molecular basis regulating the biosynthesis of artemisinin and the development of trichomes in A. annua remains poorly understood. Here, we report that an AP2 transcription factor, TRICHOME AND ARTEMISININ REGULATOR 1 （TAR1）, plays crucial roles in regulating the development of trichomes and the biosynthesis of artemisinin in A. annua. TAR1, which encodes a protein specially located in the nucleus, is mainly expressed in young leaves, flower buds, and some trichomes. In TAR1-RNAi lines, the morphology of trichomes and the composition of cuticular wax were altered, and the artemisinin content was dramatically reduced, which could be significantly increased by TAR1 oeverexpression. Expression levels of several key genes that are involved in artemisinin biosynthesis were altered when TAR1 was silenced or overexpressed. By the electrophoretic mobility shift, yeast one-hybrid and transient transformation β-glucuronidase assays, we showed that ADS and CYP71AV1, two key genes in the biosynthesis pathway of artemisinin, are likely the direct targets of TAR1. Taken together, our results indicate that TAR1 is a key component of the molecular network regulating trichome development and artemisinin biosynthesis in A. annua.展开更多
Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human health.Elucidating the mechanisms of stereoselective biosynthesis and response to stresses of lignans i...Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human health.Elucidating the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant improvement.Here,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and UGT71B2.DIR1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan content.Mechanistically,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of DIR1.These systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in I.indigotica roots and is also a part of the LTF1-mediated regulatory network to resist stresses.In conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.展开更多
Atomic structure and electronic band structure are fundamental properties for understanding the mechanism of superconductivity. Motivated by the discovery of pressure-induced high-temperature superconductivity at 80 K...Atomic structure and electronic band structure are fundamental properties for understanding the mechanism of superconductivity. Motivated by the discovery of pressure-induced high-temperature superconductivity at 80 K in the bilayer Ruddlesden-Popper nickelate La_(3)Ni_(2)O_(7), the atomic structure and electronic band structure of the trilayer nickelate La_(4)Ni_(3)O_(10) under pressure up to 44.3 GPa are investigated. A structural transition from the monoclinic P2_(1)/a space group to the tetragonal I4/mmm around 12.6-13.4 GPa is identified, accompanied by a drop of resistance below 7 K. Density functional theory calculations suggest that the bonding state of Ni 3d_(z^(2)) orbital rises and crosses the Fermi level at high pressures, which may give rise to possible superconductivity observed in resistance under pressure in La_(4)Ni_(3)O_(10). The trilayer nickelate La_(4)Ni_(3)O_(10) shows some similarities with the bilayer La_(3)Ni_(2)O_(7) and has unique properties, providing a new platform to investigate the underlying mechanism of superconductivity in nickelates.展开更多
基金the National Natural Science Foundation of China(82304659)a Chenguang Project of Shanghai(23CGA52)+2 种基金the Shanghai Municipal Science and Technology Commission 2025 Key Technology R&D Program“Synthetic Biology”Project(25HC2810300)the Key Project at Central Government Level:the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(2060302)the Science and Technology Development Program of Shanghai University of Traditional Chinese Medicine(23KFL045,23KFL051).
文摘Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicinal properties.Galloylation and glycosylation are key steps in the formation of polyphenolic compounds,as the glycosylation of flavonoids is required for the acylation of flavonoid glycosides,and the glycosylation of gallic acid is necessary for its role as an acyl donor.Therefore,glycosylation to generate the acyl donor or acceptor is a core step in the biosynthesis of polyphenolic compounds.However,how this glycosylation occurs in P.chinense is unknown.In this study,we determined that the UDP-glucose transferase PcUGT84A82 mediates the glycosylation of gallic acid and pinocembrin to produce 1-O-Galloyl-β-D-glucose and pinocembroside,respectively.Metabolic profiling of polyphenolic compounds using UHPLC-ESI–Q-TOF/MS revealed high levels of polyphenols in flowers,leaves,and roots,and low levels in stems of P.chinense.We performed isoform-sequencing(Iso-seq)to assemble a full-length transcriptome of P.chinense,from which we identified 58 UGT family members.PcUGT84A82 is highly similar to functional UGTs in other plant species,and PcUGT84A82 transcript levels were positively correlated with the levels of various polyphenolic compounds.We validated the function of PcUGT84A82 via in vitro enzyme assays and transient expression in Nicotiana benthamiana leaves.Subcellular localization tests showed that PcUGT84A82 localizes to the nucleus and cytoplasm.In summary,PcUGT84A82 catalyzes the conversion of gallic acid to 1-O-Galloyl-β-D-glucose as the acyl donor and pinocembrin to pinocembroside as the acyl acceptor,mediating the biosynthesis of galloylated macrocyclic polyphenolic compounds in P.chinense.These findings lay the foundation for elucidating the entire biosynthetic pathway of active polyphenols in this important herbal plant species.
基金supported by the National Natural Science Foundation of China(No.82272243)the Science and Technology Commission of Shanghai Municipality(No.22Y11900800).
文摘Background:Aspiration pneumonia is a severe health concern,particularly for ICU patients with impaired airway defenses.Current animal models fail to fully replicate the condition,focusing solely on chemical lung injury from gastric acid while neglecting pathogen-induced inflammation.This gap hinders research on pathogenesis and treatment,creating an urgent need for a clinically relevant model.This study aimed to develop an improved rat model of aspiration pneumonia by combining hydrochloric acid(HCl)and lipopolysaccharide(LPS)administration.Methods:Specific pathogen-free Sprague Dawley rats underwent intratracheal instillation of HCl and LPS.Techniques included rat weight measurement,tracheal intubation,pulmonary function monitoring,lung tissue sampling with HE staining and scoring,bronchoalveolar lavage fluid(BALF)sampling,protein and inflammatory cytokine analysis via BCA and ELISA,BALF pH determination,Evans Blue dye assessment,blood gas analysis,FITC-dextran leakage,Western blotting,electron microscopy,survival analysis,and transcriptome sequencing with bioinformatics.Statistical analysis was performed using GraphPad Prism.Results:The optimal model involved instillation of 1.5μL/g.wt HCl(pH=1)followed by 20μg/g.wt LPS after 1 h.This model reproduced acute lung injury,including tissue damage,pulmonary microvascular dysfunction,inflammatory responses,hypoxemia,and impaired pulmonary ventilation,with recovery observed at 72 h.PANoptosis was confirmed,characterized by increased markers.Concentration-dependent effects of HCl and LPS on lung damage were identified,alongside cytokine elevation and microvascular dysfunction.Conclusions:This optimized model closely mimics clinical aspiration pneumonia,providing a valuable tool for studying pathophysiology and therapeutic strategies.
基金Project supported by the Key-Area Research and Development Program of Guangdong Province,China(Grant No.2020B0303030002)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB0670000)the National Key Research and Development Program of China(Grant No.2023YFB4404904).
文摘High-quality entangling gates are crucial for scalable quantum information processing.Implementing all-microwave two-qubit gates on fixed-frequency transmons offers advantages in reducing wiring complexity,but the gate performance is often limited due to the residual ZZ interaction and the frequency crowding problem.Here,we introduce a novel scheme that enables a microwave drive-activated CZ gate compatible with the coupler structure to suppress the residual ZZ interaction.The microwave drive is applied to the coupler and the microwave drive frequency remains far detuned from the system’s transition frequency to alleviate the frequency crowding problem.We model the gate process analytically and demonstrate a theoretical gate fidelity up to 99.9%numerically.Our scheme is compatible with current coupler-structure-based circuits,and insensitive to microwave crosstalk,showing a possible path for all-microwave quantum operations at scale.
基金financially supported by the National Science Fund for Distinguished Young Scholars(No.62125502)the National Natural Science Foundation of China(No.51972113)+4 种基金the Key Program of Guangzhou Scientific Research Special Project(No.201904020013)the Key R&D Program of Guangzhou(No.202007020003)the Science and Technology Project of Guangdong Province(No.2021A0505030004)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01×137)the Fundamental Research Funds for the Central University。
文摘The construction of scintillating ceramics is of great technological importance for various fundamental applications, including medical diagnostic, security inspection, resource exploration and particle physics. The chief challenge is the facile and scalable synthesis of scintillating ceramics with the desirable combination of pore-free, reliable mechanical properties and excellent scintillating performance. Here we present a pressureless glass crystallization strategy for the construction of scintillating composite with high crystallinity. The fabricated scintillating composites are featured by small optical turbidity, excellent mechanical properties, and efficient scintillating luminescence with the scintillating light yield of 15,000 pH/MeV and about 2.46 times higher than that of the commercial BGO single crystal. Moreover, the scintillating composite derived radiation detector device is successfully elaborated. The practical application for monitoring gamma ray is demonstrated and the precision of the device is less than that of the tolerable deviation of 30%. Our results suggest an innovative approach for expanding the category of scintillating material candidates, pointing to practical application in the field of radiation detection.
基金financially supported by the National Nature Science Foundation of China(Grant Nos.51771064,51971079,and 51501040)。
文摘Heterostructured(HS)material with extraordinary mechanical properties has been regarded as one of the most promising structural materials.Here,we reported a new strategy for preparing heterostructured pure titanium laminates that possess a good combination of strength and ductility by combining gradient structure(GS)and heterogeneous lamella structure(HLS).The deformation characteristic versus microstructure evolution of GS/HLS titanium laminates,namely the strain partitions between different-sized grains(480–25μm)was visualized using a scanning electron microscope(SEM)equipped with electron backscattered diffraction(EBSD)mode combined with the digital image correlation(SEM-DIC)with an ultrahigh spatial resolution for the first time.As a result,the hetero-deformation of unique GS/HLS structure by the characteristic of strain partitions could be accurately captured.While the hetero-deformation could result in the hetero-deformation induced(HDI)stress strengthening and HDI hardening,which were regarded as the key reason that the resulting GS/HLS Ti laminates showed a superior combination of strength and ductility.This could promote a more in-depth understanding of the strengtheningtoughening mechanism of heterostructured material.
文摘The discovery of high-temperature superconductivity in La_(3)Ni_(2)O_(7) and La_(4)Ni_(3)O_(10) under pressure has garnered extensive attention.Herein,we report systematic investigations on the structure,magnetism,and electrical resistance evolutions of Pr_(4)Ni_(3)O_(10−δ)polycrystalline samples under various pressures.Pr_(4)Ni_(3)O_(10−δ)exhibits density wave transitions on Ni and Pr sublattices at approximately 157.6 and 4.3 K,respectively.The density wave can be progressively suppressed by pressure.A structural transformation from the monoclinic P21/a space group to the tetragonal I4/mmm occurs around 20GPa.An apparent drop in resistance with an evident magnetic field dependence is observed at pressures above 20GPa,indicating the emergence of superconductivity.The discovery of superconductivity in Pr_(4)Ni_(3)O_(10−δ)broadens the family of nickelate superconductors.Pr_(4)Ni_(3)O_(10−δ)provides a new platform for investigation of the mechanisms of superconductivity in the Ruddlesden-Popper phases of nickelates.
基金Supported by the National Natural Science Foundation of China(Nos.31971503,31901188)the Shandong Provincial Agricultural Fine Species Project(No.2019LZGC020)+5 种基金the Jining Key Research and Development Project of Shandong Province(No.2019ZDGH019)the Shandong Provincial Natural Science Foundation(Nos.ZR2019BB040,ZR2020MC042)the Interdisciplinary Project of Qufu Normal University(No.XKJJC201903)the Key Research and Development Project of Liaoning Province(No.2018228004)the Revitalization Talents Program of Liaoning Province(No.XLYC1907109),the Shandong Provincial Key Research and Development Project(No.2018GSF117035)the Shandong Provincial Higher Educational Science and Technology Program(No.J17KA112)。
文摘Algicidal bacteria have been frequently isolated from algal blooming areas.However,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is limited.In this study,we investigated the composition of the microbial community coexisting with harmful alga Karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in microcosms.The microorganisms inhabiting the K.mikimotoi culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal cultivation.Both the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of K.mikimotoi and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas increased.Core operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the K.mikimotoi culture with or without treatments.Collectively,this study indicated that microbial community inhabiting K.mikimotoi culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of K.mikimotoi via nutrient stimulation or by adding isolated algicidal strains.
基金supported by the National Natural Science Foundation of China (Nos.61503082 and 61403121)the Natural Science Foundation of Fujian Province (No. 2016J05145)+3 种基金the Fundamental Research Funds for the Central Universities (No. 2015B20214)the Program for New Century Excellent Talents in Fujian Province University (No. GY-Z18155)the Program for Outstanding Young Scientific Researcher in Fujian Province University (No. GY-Z160149)the Scientific Research Foundation of Fujian University of Technology (No. GY-Z17162).
文摘To support semantic inter-operability between the biomedical information systems, it is necessary to determine the correspondences between the heterogeneous biomedical concepts, which is commonly known as biomedical ontology matching. Biomedical concepts are usually complex and ambiguous, which makes matching biomedical ontologies a challenge. Since none of the similarity measures can distinguish the heterogeneous biomedical concepts in any context independently, usually several similarity measures are applied together to determine the biomedical concepts mappings. However, the ignorance of the effects brought about by different biomedical concept mapping’s preference on the similarity measures significantly reduces the alignment’s quality. In this study, a non-dominated sorting genetic algorithm (NSGA)-III-based biomedical ontology matching technique is proposed to effectively match the biomedical ontologies, which first utilises an ontology partitioning technique to transform the large-scale biomedical ontology matching problem into several ontology segment-matching problems, and then uses NSGA-III to determine the optimal alignment without tuning the aggregating weights. The experiment is conducted on the anatomy track and large biomedic ontologies track which are provided by the Ontology Alignment Evaluation Initiative (OAEI), and the comparisons with OAEI’s participants show the effectiveness of the authors’ approach.
基金supported by the National Natural Science Foundation of China(NSFC) under Contract No.10775055the special funds of State Key Laboratory of Advanced Elcctromag-netic Engineering and Technology,Huazhong University of Science and Technology(Grant No. 2014ZZ001)
文摘A kind of dielectric barrier discharge（DBD） device composed of water electrodes with 3×3forms can produce large-area low-temperature plasmas at atmospheric pressure.To reflect the discharge characteristics of DBD better,a dynamic simulation model,which is based on the voltage controlled current source（CCS）,is established,then the established model in Matlab/Simulink is used to simulate the DBD in air.The voltage-current waves and Lissajous at a voltage of 10 kV,11 kV and 12 kV peak value with a frequency of 15 kHz are studied.The change of the discharge power of DBD with a different amplitude and frequency of applied voltage is also analyzed.The result shows the voltage-current waves,Lissajous and discharge power of DBD under different conditions from the simulation agree well with those of the experiment.In addition,we propose a method to calculate the dielectric barrier capacitance Cd and the gap capacitance Cg,which is valid through analyzing the variation of capacitance at different voltage amplitudes.
基金financially supported by the National Key Research and Development Program of China(2022YFC3501700)the National Natural Science Foundation of China(82304662,82374159,32070327,32170402)+3 种基金the National Key Research and Development Program of China(2023YFC3504800)Chenguang project of Shanghai(23CGA52)Science and Technology Development Program of Shanghai University of Traditional Chinese Medicine(23KFL051)Shanghai Municipal Science and Technology Commission(23XD1423500)。
文摘Plants have mechanisms to transport secondary metabolites from where they are biosynthesized to the sites where they function,or to sites such as the vacuole for detoxification.However,current research has mainly focused on metabolite biosynthesis and regulation,and little is known about their transport.Tanshinone,a class diterpenoid with medicinal properties,is biosynthesized in the periderm of Salvia miltiorrhiza roots.Here,we discovered that tanshinone can be transported out of peridermal cells and secreted into the soil environment and that the ABC transporter SmABCG1 is involved in the efflux of tanshinoneⅡA and tanshinoneⅠ.The SmABCG1 gene is adjacent to the diterpene biosynthesis gene cluster in the S.miltiorrhiza genome.The temporal–spatial expression pattern of SmABCG1 is consistent with tanshinone accumulation profiles.SmABCG1 is located on the plasma membrane and preferentially accumulates in the peridermal cells of S.miltiorrhiza roots.Heterologous expression in Xenopus laevis oocytes demonstrated that SmABCG1 can export tanshinoneⅡA and tanshinoneⅠ.CRISPR/Cas9-mediated mutagenesis of SmABCG1 in S.miltiorrhiza hairy roots resulted in a significant decrease in tanshinone contents in both hairy roots and the culture medium,whereas overexpression of this gene resulted in increased tanshinone contents.CYP76AH3 transcript levels increased in hairy roots overexpressing SmABCG1 and decreased in knockout lines,suggesting that SmABCG1 may affect the expression of CYP76AH3,indirectly regulating tanshinone biosynthesis.Finally,tanshinoneⅡA showed cytotoxicity to Arabidopsis roots.These findings offer new perspectives on plant diterpenoid transport and provide a new genetic tool for metabolic engineering and synthetic biology research.
基金National Key Research and Development Program of China,grant 2023YFC3504800(to Y.X.)National Natural Science Foundation of China,grant 82470379(to S.Q.)+7 种基金Young Elite Scientists Sponsorship Program by Cast,grant 2021-QNRC1-02(to S.Q.)Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine,grant 2023YZZ02(to W.S.C.)The Open Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources,grant PFGR202501(to S.Q.)The National Natural Science Foundation of China,grant 82204577(to R.R.G.)The Research Project of Science and Technology Commission of Shanghai Municipality,grant 21DZ2202300(to S.Q.)Key project at the central government level:“The ability establishment of sustainable use for valuable Chinese medicine resources,”grant 2060302(to S.Q.)The Natural Science Foundation of Shanghai,grant 22ZR1479500(to Q.Z.)The China Postdoctoral Science Foundation,grant 2023M744290(to J.D.H.).
文摘Flavonoids,the largest class of polyphenols,exhibit substantial structural and functional diversity,yet their evolutionary diversification and specialized functions remain largely unexplored.The genus Scutellaria is notable for its rich flavonoid diversity,particularly of 6/8-hydroxylated variants biosynthesized by the cytochrome P450 subfamily CYP82D.Our study analyzes metabolic differences between Scutellaria baicalensis and Scutellaria barbata,and the results suggest that CYP82Ds have acquired a broad range of catalytic functions over their evolution.By integrating analyses of metabolic networks and gene evolution across 22 Scutellaria species,we rapidly identified 261 flavonoids and delineated five clades of CYP82Ds associated with various catalytic functions.This approach revealed a unique catalytic mode for 6/8-hydroxylation of flavanone substrates and the first instance of 7-O-demethylation of flavonoid substrates catalyzed by a cytochrome P450.Ancestral sequence reconstruction and functional validation demonstrated that gradual neofunctionalization of CYP82Ds has driven the chemical diversity of flavonoids in the genus Scutellaria throughout its evolutionary history.These findings enhance our understanding of flavonoid diversity,reveal the intricate roles of CYP82Ds in Scutellaria species,and highlight the extensive catalytic versatility of cytochrome P450 members within plant taxa.
基金supported by the National Key Research and Development Program of China(2022YFB3304701)the National Natural Science Foundation of China(62173145,62322303,62303186)+2 种基金the Natural Science Foundation of Shanghai under Grant 24ZR1415900the Shanghai Pilot Program for Basic Research(22TQ1400100-16)the State Key Laboratory of Industrial Control Technology,China(Grant No.ICT2024A26).
文摘Data-driven process monitoring methods are widely used in industrial tasks,with visual monitoring enabling operators to intuitively understand operational status,which is vital for maximizing industrial safety and production efficiency.However,high-dimensional industrial data often exhibit complex structures,making the traditional 2D visualization methods ineffective at distinguishing different fault types.Thus,a visual process monitoring method that combines supervised uniform manifold approximation and projection with a label assignment strategy is proposed herein.First,the proposed supervised projection method enhances the visualization step by incorporating label information to guide the nonlinear dimensionality reduction process,improving the degrees of class separation and intraclass compactness.Then,to address the lack of label information for online samples,a label assignment strategy is designed.This strategy integrates kernel Fisher discriminant analysis and Bayesian inference,assigning different label types to online samples based on their confidence levels.Finally,upon integrating the label assignment strategy with the proposed supervised projection method,the assigned labels enhance the separability of online projections and enable the visualization of unknown data to some extent.The proposed method is validated on the Tennessee Eastman process and a real continuous catalytic reforming process,demonstrating superior visual fault monitoring and diagnosis performance to that of the state-of-the-art methods,especially in real industrial applications.
文摘Trichomes, small protrusions on the surface of many plant species, can produce and store various secondary metabolic products. Artemisinin, the most famous and potent medicine for malaria, is synthesized, stored, and secreted by Artemisia annua trichomes. However, the molecular basis regulating the biosynthesis of artemisinin and the development of trichomes in A. annua remains poorly understood. Here, we report that an AP2 transcription factor, TRICHOME AND ARTEMISININ REGULATOR 1 （TAR1）, plays crucial roles in regulating the development of trichomes and the biosynthesis of artemisinin in A. annua. TAR1, which encodes a protein specially located in the nucleus, is mainly expressed in young leaves, flower buds, and some trichomes. In TAR1-RNAi lines, the morphology of trichomes and the composition of cuticular wax were altered, and the artemisinin content was dramatically reduced, which could be significantly increased by TAR1 oeverexpression. Expression levels of several key genes that are involved in artemisinin biosynthesis were altered when TAR1 was silenced or overexpressed. By the electrophoretic mobility shift, yeast one-hybrid and transient transformation β-glucuronidase assays, we showed that ADS and CYP71AV1, two key genes in the biosynthesis pathway of artemisinin, are likely the direct targets of TAR1. Taken together, our results indicate that TAR1 is a key component of the molecular network regulating trichome development and artemisinin biosynthesis in A. annua.
基金The authors would like to acknowledge Professor Jiankang Zhu and his lab at the Shanghai Center for Plant Stress Biology,Chinese Center for Plant Stress Biology,for providing the CRISPR/Cas9 system plasmids.All authors declare no competing financial or nonfinancial interests.This work was funded by the National Natural Science Foundation of China(grant Nos.82225047,32000231,31970316,and 32170274)the National Key Research and Development Program of China(grant no.2022YFC3501703)+1 种基金Shanghai Science and Technology Development Funds(23QA1411400,China)Key project at central government level(The ability establishment of sustainable use for valuable Chinese medicine resources,2060302).
文摘Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human health.Elucidating the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant improvement.Here,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and UGT71B2.DIR1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan content.Mechanistically,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of DIR1.These systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in I.indigotica roots and is also a part of the LTF1-mediated regulatory network to resist stresses.In conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.
基金supported by the National Natural Science Foundation of China (Grant Nos.12174454,12304187,U213010013,92165204,and 11974432)Guangdong Basic and Applied Basic Research Funds (Grant No.2021B1515120015)+5 种基金Guangzhou Basic and Applied Basic Research Funds (Grant Nos.202201011123,and 2024A04J6417)National Key Research and Development Program of China (Grant Nos.2022YFA1402802,2023YFA1406500,and 2023YFA1406002)Guangdong Provincial Key Laboratory of Magnetoelectric Physics and Devices (Grant No.2022B1212010008)Fundamental Research Funds for the Central Universities,Sun Yat-sen University (Grant No.23qnpy57)Shenzhen International Quantum AcademyHigh-pressure synchrotron X-ray measurements were performed at the 4W2 High-Pressure Station,Beijing Synchrotron Radiation Facility,which is supported by the Chinese Academy of Sciences (Grant Nos.KJCX2-SW-N20,and KJCX2-SW-N03)。
文摘Atomic structure and electronic band structure are fundamental properties for understanding the mechanism of superconductivity. Motivated by the discovery of pressure-induced high-temperature superconductivity at 80 K in the bilayer Ruddlesden-Popper nickelate La_(3)Ni_(2)O_(7), the atomic structure and electronic band structure of the trilayer nickelate La_(4)Ni_(3)O_(10) under pressure up to 44.3 GPa are investigated. A structural transition from the monoclinic P2_(1)/a space group to the tetragonal I4/mmm around 12.6-13.4 GPa is identified, accompanied by a drop of resistance below 7 K. Density functional theory calculations suggest that the bonding state of Ni 3d_(z^(2)) orbital rises and crosses the Fermi level at high pressures, which may give rise to possible superconductivity observed in resistance under pressure in La_(4)Ni_(3)O_(10). The trilayer nickelate La_(4)Ni_(3)O_(10) shows some similarities with the bilayer La_(3)Ni_(2)O_(7) and has unique properties, providing a new platform to investigate the underlying mechanism of superconductivity in nickelates.
