Objective:To study the inhibition effect of miR-106 a inhibitor on tumor growth of ovarian cancer xenografts mice.Methods:BALB/c mice were selected as experimental animals,ovarian cancer SKOV-3 cells transfected with ...Objective:To study the inhibition effect of miR-106 a inhibitor on tumor growth of ovarian cancer xenografts mice.Methods:BALB/c mice were selected as experimental animals,ovarian cancer SKOV-3 cells transfected with miR-106 a inhibitor and its negative control were inoculated subcutaneously,intratumoral injection of miR-106 a inhibitor and its negative control were continued after tumor formation,and they were enrolled as treatment group and model group,respectively.Tumor volume and weight as well as Ki-67 and programmed cell death 4(PDCD4) expression were determined;miR-106 a inhibitor and its negative control as well as miR-106 a mimic and its negative control were transfected into SKOV-3 cells,and expression of PDCD4 in cells was determined.Results:Tumor tissue volume and weight as well as mR NA expression and protein expression of Ki-67 in treatment group were significantly lower than those in the model group while m RNA expression and protein expression of PDCD4 were significantly higher than those in the model group;transfection of mi R-106 a mimic could decrease m RNA expression and protein expression of PDCD4 in SKOV-3 cells,and transfection of miR-106 a inhibitor could increase mR NA expression and protein expression of PDCD4 in SKOV-3 cells.Conclusions:Transfection of mi R-106 a inhibitor can inhibit the growth of tumor in ovarian cancer xenografts mice through increasing the expression of PDCD4.展开更多
Objective: To study the effects of VEP neoadjuvant chemotherapy before esophageal cancer surgery on the malignant biological behaviors of tumor cells. Methods: Patients who were diagnosed with locally advanced esophag...Objective: To study the effects of VEP neoadjuvant chemotherapy before esophageal cancer surgery on the malignant biological behaviors of tumor cells. Methods: Patients who were diagnosed with locally advanced esophageal cancer in Dangyang People's Hospital between March 2015 and March 2017 were selected as the research objects and randomly divided into the VEP group who received preoperative VEP (etoposide + 5-fluorouracil + cisplatin) chemotherapy and the control group who accepted routine preoperative preparation. The serum contents of tumor markers were determined at diagnosis and 1 d before surgery;the expression of proliferation genes and invasion genes in tumor tissue were determined after surgical resection. Results: One day before surgery, serum CEA, CA125, CYFRA21-1 and SCC-Ag levels of VEP group were significantly lower than those at diagnosis, and serum CEA, CA125, CYFRA21-1 and SCC-Ag levels of control group were not significantly different from those at diagnosis;after surgical resection, PTEN, Smac and PTPN14 mRNA expressions in the tumor tissue of VEP group were significantly higher than those of control group whereas CyclinB1, CDK1, Grp94, MMP2, β-catenin, Slug, Vimentin and N-cadherin mRNA expressions were significantly lower than those of control group. Conclusions: VEP neoadjuvant chemotherapy before esophageal cancer surgery can reduce the growth of tumor cells and inhibit the proliferation and invasion of tumor cells in the lesion.展开更多
Objective: To study the correlation of miR-451 expression with proliferation and apoptosis gene expression in ovarian cancer. Methods: The patients with ovarian cancer who underwent surgical resection in our hospital ...Objective: To study the correlation of miR-451 expression with proliferation and apoptosis gene expression in ovarian cancer. Methods: The patients with ovarian cancer who underwent surgical resection in our hospital between March 2015 and February 2018 were selected, ovarian cancer lesions and adjacent lesions were collected, miRNA was isolated to determine the expression of miR-451, and mRNA was isolated to determine the mRNA expression of cyclin, proliferation genes and apoptosis genes. Results: The expression of miR-451 as well as the mRNA expression of Cyclin-dependent kinase inhibitor 1B (p27Kip1), BCL2-associated X protein (Bax), factor associated suicide (Fas), Fas ligand (FasL), cysteine aspartyl proteinase 3 (Caspase-3) and CCAAT/enhancer-binding protein homologous protein (CHOP) in ovarian cancer lesions was significantly lower than those in adjacent lesions while the mRNA expression of CyclinB1, CyclinD1, CyclinE, phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), β-catenin, matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP 9) were significantly higher than those in adjacent lesions;miR-451 in ovarian cancer was negatively correlated with CyclinB1, CyclinD1, CyclinE, PI3K, AKT, β-catenin, MMP2 and MMP9 mRNA expression, and positively correlated with p27Kip1, Bax, Fas, FasL, Caspase-3 and CHOP mRNA expression. Conclusion: The low expression of miR-451 in ovarian cancer is closely correlated with the high expression of positive cell cycle regulatory proteins and proliferation genes as well as the low expression of negative cell cycle regulatory proteins and apoptosis genes, and it may be the key factor to promote the growth of ovarian cancer.展开更多
基金supported by Science and Technology Program of Hebei Province in 2013 (No.132777163)
文摘Objective:To study the inhibition effect of miR-106 a inhibitor on tumor growth of ovarian cancer xenografts mice.Methods:BALB/c mice were selected as experimental animals,ovarian cancer SKOV-3 cells transfected with miR-106 a inhibitor and its negative control were inoculated subcutaneously,intratumoral injection of miR-106 a inhibitor and its negative control were continued after tumor formation,and they were enrolled as treatment group and model group,respectively.Tumor volume and weight as well as Ki-67 and programmed cell death 4(PDCD4) expression were determined;miR-106 a inhibitor and its negative control as well as miR-106 a mimic and its negative control were transfected into SKOV-3 cells,and expression of PDCD4 in cells was determined.Results:Tumor tissue volume and weight as well as mR NA expression and protein expression of Ki-67 in treatment group were significantly lower than those in the model group while m RNA expression and protein expression of PDCD4 were significantly higher than those in the model group;transfection of mi R-106 a mimic could decrease m RNA expression and protein expression of PDCD4 in SKOV-3 cells,and transfection of miR-106 a inhibitor could increase mR NA expression and protein expression of PDCD4 in SKOV-3 cells.Conclusions:Transfection of mi R-106 a inhibitor can inhibit the growth of tumor in ovarian cancer xenografts mice through increasing the expression of PDCD4.
文摘Objective: To study the effects of VEP neoadjuvant chemotherapy before esophageal cancer surgery on the malignant biological behaviors of tumor cells. Methods: Patients who were diagnosed with locally advanced esophageal cancer in Dangyang People's Hospital between March 2015 and March 2017 were selected as the research objects and randomly divided into the VEP group who received preoperative VEP (etoposide + 5-fluorouracil + cisplatin) chemotherapy and the control group who accepted routine preoperative preparation. The serum contents of tumor markers were determined at diagnosis and 1 d before surgery;the expression of proliferation genes and invasion genes in tumor tissue were determined after surgical resection. Results: One day before surgery, serum CEA, CA125, CYFRA21-1 and SCC-Ag levels of VEP group were significantly lower than those at diagnosis, and serum CEA, CA125, CYFRA21-1 and SCC-Ag levels of control group were not significantly different from those at diagnosis;after surgical resection, PTEN, Smac and PTPN14 mRNA expressions in the tumor tissue of VEP group were significantly higher than those of control group whereas CyclinB1, CDK1, Grp94, MMP2, β-catenin, Slug, Vimentin and N-cadherin mRNA expressions were significantly lower than those of control group. Conclusions: VEP neoadjuvant chemotherapy before esophageal cancer surgery can reduce the growth of tumor cells and inhibit the proliferation and invasion of tumor cells in the lesion.
文摘Objective: To study the correlation of miR-451 expression with proliferation and apoptosis gene expression in ovarian cancer. Methods: The patients with ovarian cancer who underwent surgical resection in our hospital between March 2015 and February 2018 were selected, ovarian cancer lesions and adjacent lesions were collected, miRNA was isolated to determine the expression of miR-451, and mRNA was isolated to determine the mRNA expression of cyclin, proliferation genes and apoptosis genes. Results: The expression of miR-451 as well as the mRNA expression of Cyclin-dependent kinase inhibitor 1B (p27Kip1), BCL2-associated X protein (Bax), factor associated suicide (Fas), Fas ligand (FasL), cysteine aspartyl proteinase 3 (Caspase-3) and CCAAT/enhancer-binding protein homologous protein (CHOP) in ovarian cancer lesions was significantly lower than those in adjacent lesions while the mRNA expression of CyclinB1, CyclinD1, CyclinE, phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), β-catenin, matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP 9) were significantly higher than those in adjacent lesions;miR-451 in ovarian cancer was negatively correlated with CyclinB1, CyclinD1, CyclinE, PI3K, AKT, β-catenin, MMP2 and MMP9 mRNA expression, and positively correlated with p27Kip1, Bax, Fas, FasL, Caspase-3 and CHOP mRNA expression. Conclusion: The low expression of miR-451 in ovarian cancer is closely correlated with the high expression of positive cell cycle regulatory proteins and proliferation genes as well as the low expression of negative cell cycle regulatory proteins and apoptosis genes, and it may be the key factor to promote the growth of ovarian cancer.
