Allopolyploids often exhibit advantages in vigor and adaptability compared to diploids.A long-term goal in the economically important Brassica genus has been to develop a new allohexaploid crop type(AABBCC)by combinin...Allopolyploids often exhibit advantages in vigor and adaptability compared to diploids.A long-term goal in the economically important Brassica genus has been to develop a new allohexaploid crop type(AABBCC)by combining different diploid and allotetraploid crop species.However,early-generation allohexaploids often face challenges like unstable meiosis and low fertility,and the phenotypic performance of these synthetic lines has rarely been assessed.This study analyzes agronomic traits,fertility,and genome stability in ArArBcBcCcCc lines derived from four crosses between B.carinata and B.rapa after 9–11 selfing generations.Our results demonstrate polyploid advantage in vigor and seed traits,considerable phenotypic variation,and high fertility and genome stability.Meanwhile,parental genotypes significantly influence outcomes in advanced allohexaploids.Structural variants,largely resulting from A–C homoeologous exchanges,contribute to genomic variation and influence hexaploid genome stability,with the A sub-genome showing the highest variability.Both positive and negative impacts of SVs on fertility and seed weight are observed.Pseudo-euploids,frequently appearing,do not significantly affect fertility or other agronomic traits compared to euploids,indicating a potential pathway toward a stable allohexaploid species.These findings provide insights into the challenge and potential for developing an adaptable and stable Brassica hexaploid through selection.展开更多
In most types of erectile dysfunction,particularly in advanced stages,typical pathological features observed are reduced parenchymal cells coupled with increased tissue fibrosis.However,the current treatment methods h...In most types of erectile dysfunction,particularly in advanced stages,typical pathological features observed are reduced parenchymal cells coupled with increased tissue fibrosis.However,the current treatment methods have shown limited success in reversing these pathologic changes.Recent research has revealed that changes in autophagy levels,along with alterations in apoptosis and fibrosis-related proteins,are linked to the progression of erectile dysfunction,suggesting a significant association.Autophagy,known to significantly affect cell fate and tissue fibrosis,is currently being explored as a potential treatment modality for erectile dysfunction.However,these present studies are still in their nascent stage,and there are limited experimental data available.This review analyzes erectile dysfunction from a pathological perspective.It provides an in-depth overview of how autophagy is involved in the apoptotic processes of smooth muscle and endothelial cells and its role in the fibrotic processes occurring in the cavernosum.This study aimed to develop a theoretical framework for the potential effectiveness of autophagy in preventing and treating erectile dysfunction,thus encouraging further investigation among researchers in this area.展开更多
Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,th...Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,the additional functions of the bone vasculature,such as the secretion of various factors and the regulation of bone-related signaling pathways,are essential for maintaining bone health.In the bone microenvironment,bone tissue cells play a critical role in regulating angiogenesis,including osteoblasts,bone marrow mesenchymal stem cells(BMSCs),and osteoclasts.Osteogenesis and bone angiogenesis are closely linked.The decrease in osteogenesis and bone angiogenesis caused by aging leads to osteoporosis.Long noncoding RNAs(lncRNAs)are involved in various physiological processes,including osteogenesis and angiogenesis.Recent studies have shown that lncRNAs could mediate the crosstalk between angiogenesis and osteogenesis.However,the mechanism by which lncRNAs regulate angiogenesis-osteogenesis crosstalk remains unclear.In this review,we describe in detail the ways in which lncRNAs regulate the crosstalk between osteogenesis and angiogenesis to promote bone health,aiming to provide new directions for the study of the mechanism by which lncRNAs regulate bone metabolism.展开更多
It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-...It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-reperfusion injury in rabbits using somatosensory evoked potential detection technology. The results showed that the somatosensory evoked potential latency was significantly prolonged and the amplitude significantly reduced until it disappeared during the period of spinal cord ischemia. After reperfusion for 30-180 minutes, the amplitude and latency began to gradually recover; at 360 minutes of reperfusion, the latency showed no significant difference compared with the pre-ischemic value, while the somatosensory evoked potential amplitude in- creased, and severe hindlimb motor dysfunctions were detected. Experimental findings suggest that changes in somatosensory evoked potentia~ ~atency can reflect the degree of spinat cord ischemic injury, while the amplitude variations are indicators of the late spinal cord reperfusion injury, which provide evidence for the assessment of limb motor function and avoid iatrogenic spinal cord injury.展开更多
AIM:To report the incidence,clinical features and outcomes of gastrointestinal(GI)involvement in Behcet’s disease(BD).METHODS:A total of 168 consecutive patients with BD were screened and upper and lower GI endoscopi...AIM:To report the incidence,clinical features and outcomes of gastrointestinal(GI)involvement in Behcet’s disease(BD).METHODS:A total of 168 consecutive patients with BD were screened and upper and lower GI endoscopies were performed in 148 patients.Four hundred age-and sex-matched controls were enrolled for comparison.RESULTS:Fifty-two(35.1%)patients had GI lesions.After a mean follow-up of 10 mo,ileocecal ulcers had been confirmed in 20 patients,including active ulcer(s)in 18 patients,but no ileocecal ulceration was found in controls.GI symptoms were present in 14 patients with active ulcer(s),while 4 patients with smaller ulcer were asymptomatic.Endoscopic features of ileocecalulcer were:a single ulcer(50%),larger than 1 cm in diameter(72.2%),and round/oval or volcano-type in shape(83.3%).Compared with patients without GI involvement,less ocular lesions,lower levels of albumin,erythrocyte count and hemoglobin,and higher levels of C-reactive protein and erythrocyte sedimentation rate were confirmed in the intestinal BD group.Four patients had esophageal ulcers in the BD group but no case in controls.The other endoscopic findings were similar between the two groups.The prevalence of Helicobacter pylori infection was similar in both groups.Most patients received an immunomodulator and responded well.CONCLUSION:GI lesions commonly occur in Chinese BD patients.The most frequently involved area is the ileocecal region.Esophageal ulcer might be a rare but unique lesion.展开更多
Radiative aerosols are known to influence the surface energy budget and hence the evolution of the planetary boundary layer. In this study, we develop a method to estimate the aerosol-induced reduction in the planetar...Radiative aerosols are known to influence the surface energy budget and hence the evolution of the planetary boundary layer. In this study, we develop a method to estimate the aerosol-induced reduction in the planetary boundary layer height （PBLH） based on two years of ground-based measurements at a site, the Station for Observing Regional Processes of the Earth System （SORPES）, at Nanjing University, China, and radiosonde data from the meteorological station of Nanjing. The observations show that increased aerosol loads lead to a mean decrease of 67.1 W m-2 for downward shortwave radiation （DSR） and a mean increase of 19.2 W m-2 for downward longwave radiation （DLR）, as well as a mean decrease of 9.6 W m-2 for the surface sensible heat flux （SHF） in the daytime. The relative variations of DSR, DLR and SHF are shown as a function of the increment of column mass concentration of particulate matter （PM2.5）. High aerosol loading can significantly increase the atmospheric stability in the planetary boundary layer during both daytime and nighttime. Based on the statistical relationship between SHF and PM2.5 column mass concentrations, the SHF under clean atmospheric conditions （same as the background days） is derived. In this case, the derived SHF, together with observed SHF, are then used to estimate changes in the PBLH related to aerosols. Our results suggest that the PBLH decreases more rapidly with increasing aerosol loading at high aerosol loading. When the daytime mean column mass concentration of PM2.5 reaches 200 mg m-2, the decrease in the PBLH at 1600 LST （local standard time） is about 450 m.展开更多
The traditional Chinese medicine(TCM)theory of traditional Chinese health exercise(TCHE)is analyzed.The effect of meridians and acupoints contained in TCHE is summarized,the treatment methods of tonifying deficiencies...The traditional Chinese medicine(TCM)theory of traditional Chinese health exercise(TCHE)is analyzed.The effect of meridians and acupoints contained in TCHE is summarized,the treatment methods of tonifying deficiencies and reducing excesses applied in TCHE are discussed,the regulation of Zang-fu organs are emphasized,and the leading role of Qi is introduced.The exercise prescription and proper timing of TCHE are described and reported.TCHEs,such as Baduanjin,Liuzijue,and Tai Chi,possess strong TCM characteristics.展开更多
Osteoarthritis(OA),characterized by cartilage degeneration,synovial inflammation,and subchondral bone remodeling,is among the most common musculoskeletal disorders globally in people over 60 years of age.The initiatio...Osteoarthritis(OA),characterized by cartilage degeneration,synovial inflammation,and subchondral bone remodeling,is among the most common musculoskeletal disorders globally in people over 60 years of age.The initiation and progression of OA involves the abnormal metabolism of chondrocytes as an important pathogenic process.Cartilage degeneration features mitochondrial dysfunction as one of the important causative factors of abnormal chondrocyte metabolism.Therefore,maintaining mitochondrial homeostasis is an important strategy to mitigate OA.Mitophagy is a vital process for autophagosomes to target,engulf,and remove damaged and dysfunctional mitochondria,thereby maintaining mitochondrial homeostasis.Cumulative studies have revealed a strong association between mitophagy and OA,suggesting that the regulation of mitophagy may be a novel therapeutic direction for OA.By reviewing the literature on mitophagy and OA published in recent years,this paper elaborates the potential mechanism of mitophagy regulating OA,thus providing a theoretical basis for studies related to mitophagy to develop new treatment options for OA.展开更多
Developing artificial muscles that can replace biological muscles to accomplish various tasks iswhat we have long been aiming for.Recent advances in flexible materials and 3D printing technology greatly promote the de...Developing artificial muscles that can replace biological muscles to accomplish various tasks iswhat we have long been aiming for.Recent advances in flexible materials and 3D printing technology greatly promote the development of artificial muscle technology.A variety of flexible material-based artificial muscles that are driven by different external stimuli,including pressure,voltage,light,magnetism,temperature,etc.,have been developed.Among these,fluid-driven artificial muscles(FAMs),which can convert the power of fluid(gas or liquid)into the force output and displacement of flexible materials,are the most widely used actuation methods for industrial robots,medical instruments,and human-assisted devices due to their simplicity,excellent safety,large actuation force,high energy efficiency,and low cost.Herein,the bio-design,manufacturing,sensing,control,and applications of FAMs are introduced,including conventional pneumatic/hydraulic artificial muscles and several innovative artificial muscles driven by functional fluids.What’s more,the challenges and future directions of FAMs are discussed.展开更多
Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It...Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It is widely recognized that circadian clock genes can regulate bone metabolism.In recent years,the regulation of bone metabolism by non-coding RNAs has become a hotspot of research.MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism,including circadian clock genes.However,research in this field has been conducted only in recent years and the mechanisms involved are not yet well established.Recent studies have focused on how to target circadian clock genes to treat some diseases,such as autoimmune diseases,but few have focused on the co-regulation of circadian clock genes and microRNAs in bone metabolic diseases.Therefore,in this paper we review the progress of research on the co-regulation of bone metabolism by circadian clock genes and microRNAs,aiming to provide new ideas for the prevention and treatment of bone metabolic diseases such as osteoporosis.展开更多
Mathematical models are been proposed to simulate the thermal and metallurgical behaviors of the strip occtLrring on the run-out table （ROT） in a hot strip mill. A variational method is utilized for the discretizati...Mathematical models are been proposed to simulate the thermal and metallurgical behaviors of the strip occtLrring on the run-out table （ROT） in a hot strip mill. A variational method is utilized for the discretization of the governing transient conduction-convection equation, with heat transfer coefficients adaptively determined by the actual mill data. To consider the thermal effect of phase transformation during cooling, a constitutive equation for describing austenite decomposition kinetics of steel in air and water cooling zones is coupled with the heat transfer model. As the basic required inputs in the numerical simulations, thermal material properties are experimentally measured for three carbon steels and the least squares method is used to statistically derive regression models for the properties, including specific heat and thermal conductivity. The numerical simulation and experimental results show that the setup accuracy of the temperature prediction system of ROT is effectively improved.展开更多
Extensive exotic introgression could significantly enlarge the genetic distance of hybrid parental populations to promote strong heterosis.The goal of this study was to investigate whether genome-wide prediction can s...Extensive exotic introgression could significantly enlarge the genetic distance of hybrid parental populations to promote strong heterosis.The goal of this study was to investigate whether genome-wide prediction can support pre-breeding in populations with exotic introgressions.We evaluated seed yield,seed yield related traits and seed quality traits of 363 hybrids of Brassica napus (AACC) derived from two parental populations divergent on massive exotic introgression of related species in three environments.The hybrids presented strong heterosis on seed yield,which was much higher than other investigated traits.Five genomic best linear unbiased prediction models considering the exotic introgression and different marker effects (additive,dominance,and epistatic effects) were constructed to test the prediction ability for different traits of the hybrids.The analysis showed that the trait complexity,exotic introgression,genetic relationship between the training set and testing set,training set size,and environments affected the prediction ability.The models with best prediction ability for different traits varied.However,relatively high prediction ability (e.g.,0.728 for seed yield) was also observed when the simplest models were used,excluding the effects of the special exotic introgression and epistasis effect by5-fold cross validation,which would simplify the prediction for the trait with complex architecture for hybrids with exotic introgression.The results provide novel insights and strategies for genome-wide prediction of hybrids between genetically distinct parent groups with exotic introgressions.展开更多
Bone is a connective tissue that has important functions in the human body.Cells and the extracellular matrix(ECM)are key components of bone and are closely related to bone-related diseases.However,the outcomes of con...Bone is a connective tissue that has important functions in the human body.Cells and the extracellular matrix(ECM)are key components of bone and are closely related to bone-related diseases.However,the outcomes of conventional treatments for bone-related diseases are not promising,and hence it is necessary to elucidate the exact regulatory mechanisms of bone-related diseases and identify novel biomarkers for diagnosis and therapy.Circular RNAs(circRNAs)are single-stranded RNAs that form closed circular structures without a 5’cap or 3’tail and polycyclic adenylate tails.Due to their high stability,circRNAs have the potential to be typical biomarkers.Accumulating evidence suggests that circRNAs are involved in bone-related diseases,including osteoarthritis,osteoporosis,osteosarcoma,multiple myeloma,intervertebral disc degeneration,and rheumatoid arthritis.Herein,we summarize the recent research progress on the characteristics and functions of circRNAs,and highlight the regulatory mechanism of circRNAs in bone-related diseases.展开更多
We proposed an optimal design method to expand the bandwidth for the control of large hydraulic Stewart platform.The method is based on generalized natural frequency and takes hydraulic oil into consideration.A Lagran...We proposed an optimal design method to expand the bandwidth for the control of large hydraulic Stewart platform.The method is based on generalized natural frequency and takes hydraulic oil into consideration.A Lagrangian formulation which considers the whole leg inertia is presented to obtain the accurate equivalent mass matrix.Using the model,the effect of leg inertia and the influence of design parameters on the generalized natural frequency are investigated.Finally,numerical examples are presented to validate and confirm the efficiency of the mathematical model.The results show that the leg inertia,especially the piston part plays an important role in the dynamics.The optimum diameter ratio of the base to the moving platform is between 2 and 3,and the optimum joint angle ratio of the base to the moving platform is about 1.The smaller joint angles and a longer leg stroke are favorable for raising system frequencies.The system oil should be preprocessed for large platforms with a requirement for good dynamic performance.展开更多
Naturally occurred precore(PC,G1896A)and/or basal core promoter(BCP,A1762T/G1764A)mutations are prevalent in chronic HBV-infected patients,especially those under HBeAg-negative status.However,the replicative capacity ...Naturally occurred precore(PC,G1896A)and/or basal core promoter(BCP,A1762T/G1764A)mutations are prevalent in chronic HBV-infected patients,especially those under HBeAg-negative status.However,the replicative capacity of HBV with PC/BCP mutations remains ambiguous.Herein,meta-analysis showed that,only under HBeAg-negative status,the serum HBV DNA load in patients with PC mutation was 7.41-fold higher than those without the mutation.Both PC mutation alone and BCPþPC mutations promoted HBV replication in cell and hydrodynamic injection mouse models.In human hepatocyte chimeric mouse model,BCPþPC mutations led to elevated replicative capacity and intrahepatic core protein accumulation.Mechanistically,preC RNA harboring PC mutation could serve as mRNA to express core and P proteins,and such pgRNA-like function favored the maintenance of cccDNA pool under HBeAg-negative status.Additionally,BCPþPC mutations induced more extensive and severe human hepatocyte damage as well as activated endoplasmic reticulum stress and TNF signaling pathway in livers of chimeric mice.This study indicates that HBeAg-negative patients should be monitored on HBV mutations regularly and are expected to receive early antiviral treatment to prevent disease progression.展开更多
The poppet valve is a fundamental component in fluid power systems. Under particular conditions, annoying ＂squeal＂ noises may be generated in hydraulic poppet valves. In the present study, the frequency spectrum of ...The poppet valve is a fundamental component in fluid power systems. Under particular conditions, annoying ＂squeal＂ noises may be generated in hydraulic poppet valves. In the present study, the frequency spectrum of the squeal noise is obtained by analyzing the sampling data from the accelerometer mounted on the valve body. It is found that the flow velocity, pressure, and structural parameters have crucial effects on the properties of squeal noise, especially frequency. Larger valve chamber volume or lower backpressure leads to lower fundamental frequency of the squeal noise. An explanation for the squeal noise, as a result of Helmholtz resonance, is suggested and proved by experimental results.展开更多
Soft robotics,compared with their rigid counterparts,are able to adapt to uncharted environments,are superior in safe human-robot interactions,and have low cost,owing to the native compliance of the soft materials.How...Soft robotics,compared with their rigid counterparts,are able to adapt to uncharted environments,are superior in safe human-robot interactions,and have low cost,owing to the native compliance of the soft materials.However,customized complex structures,as well as the nonlinear and viscoelastic soft materials,pose a great challenge to accurate modeling and control of soft robotics,and impose restrictions on further applications.In this study,a unified modeling strategy is proposed to establish a complete dynamic model of the most widely used pneumatic soft bending actuator.First,a novel empirical nonlinear model with parametric and nonlinear uncertainties is identified to describe the nonlinear behaviors of pneumatic soft bending actuators.Second,an inner pressure dynamic model of a pneumatic soft bending actuator is established by introducing a modified valve flow rate model of the unbalanced pneumatic proportional valves.Third,an adaptive robust controller is designed using a backstepping method to handle and update the nonlinear and uncertain system.Finally,the experimental results of comparative trajectory tracking control indicate the validity of the proposed modeling and control method.展开更多
"Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic s..."Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic structural variation(SV).However,how such SV arises,is inherited and fixed,and how it affects important traits,has rarely been comprehensively and quantitively studied in advanced generation synthetic lines.A better understanding of these processes will aid breeders in knowing how to best utilize synthetic allopolyploids in breeding programs.Here,we analyzed three genetic mapping populations(735 DH lines)derived from crosses between advanced synthetic and conventional Brassica napus(rapeseed)lines,using whole-genome sequencing to determine genome composition.We observed high tolerance of large structural variants,particularly toward the telomeres,and preferential selection for balanced homoeologous exchanges(duplication/deletion events between the A and C genomes resulting in retention of gene/chromosome dosage between homoeologous chromosome pairs),including stable events involving whole chromosomes("pseudoeuploidy").Given the experimental design(all three populations shared a common parent),we were able to observe that parental SV was regularly inherited,showed genetic hitchhiking effects on segregation,and was one of the major factors inducing adjacent novel and larger SV.Surprisingly,novel SV occurred at low frequencies with no significant impacts on observed fertility and yield-related traits in the advanced generation synthetic lines.However,incorporating genome-wide SV in linkage mapping explained significantly more genetic variance for traits.Our results provide a framework for detecting and understanding the occurrence and inheritance of genomic SV in breeding programs,and support the use of synthetic parents as an important source of novel trait variation.展开更多
基金supported by the Sino-German Research Project(GZ 1362)Grains Research and Development Corporation International Visiting Fellowship(UWA2406-010BGX)+1 种基金the National Natural Science Foundation of China(32171982)the Fundamental Research Funds for the Central Universities of the Chinese Government(2662023PY004).
文摘Allopolyploids often exhibit advantages in vigor and adaptability compared to diploids.A long-term goal in the economically important Brassica genus has been to develop a new allohexaploid crop type(AABBCC)by combining different diploid and allotetraploid crop species.However,early-generation allohexaploids often face challenges like unstable meiosis and low fertility,and the phenotypic performance of these synthetic lines has rarely been assessed.This study analyzes agronomic traits,fertility,and genome stability in ArArBcBcCcCc lines derived from four crosses between B.carinata and B.rapa after 9–11 selfing generations.Our results demonstrate polyploid advantage in vigor and seed traits,considerable phenotypic variation,and high fertility and genome stability.Meanwhile,parental genotypes significantly influence outcomes in advanced allohexaploids.Structural variants,largely resulting from A–C homoeologous exchanges,contribute to genomic variation and influence hexaploid genome stability,with the A sub-genome showing the highest variability.Both positive and negative impacts of SVs on fertility and seed weight are observed.Pseudo-euploids,frequently appearing,do not significantly affect fertility or other agronomic traits compared to euploids,indicating a potential pathway toward a stable allohexaploid species.These findings provide insights into the challenge and potential for developing an adaptable and stable Brassica hexaploid through selection.
基金supported by the Health Commission Research Project of China(No.HDSL202001057)the Jiangxi Provincial Health Commission Research Project(No.SKJP20203656)the Doctoral Start-up Fund of the First Affiliated Hospital of Gannan Medical University(No.QD063).
文摘In most types of erectile dysfunction,particularly in advanced stages,typical pathological features observed are reduced parenchymal cells coupled with increased tissue fibrosis.However,the current treatment methods have shown limited success in reversing these pathologic changes.Recent research has revealed that changes in autophagy levels,along with alterations in apoptosis and fibrosis-related proteins,are linked to the progression of erectile dysfunction,suggesting a significant association.Autophagy,known to significantly affect cell fate and tissue fibrosis,is currently being explored as a potential treatment modality for erectile dysfunction.However,these present studies are still in their nascent stage,and there are limited experimental data available.This review analyzes erectile dysfunction from a pathological perspective.It provides an in-depth overview of how autophagy is involved in the apoptotic processes of smooth muscle and endothelial cells and its role in the fibrotic processes occurring in the cavernosum.This study aimed to develop a theoretical framework for the potential effectiveness of autophagy in preventing and treating erectile dysfunction,thus encouraging further investigation among researchers in this area.
基金supported by the National Natural Science Foundation of China(No.81901430)the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010379)+1 种基金the Science and Technology Projects in Guangzhou(No.2023A04J0555)the Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion(No.2021B1212040014),China.
文摘Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,the additional functions of the bone vasculature,such as the secretion of various factors and the regulation of bone-related signaling pathways,are essential for maintaining bone health.In the bone microenvironment,bone tissue cells play a critical role in regulating angiogenesis,including osteoblasts,bone marrow mesenchymal stem cells(BMSCs),and osteoclasts.Osteogenesis and bone angiogenesis are closely linked.The decrease in osteogenesis and bone angiogenesis caused by aging leads to osteoporosis.Long noncoding RNAs(lncRNAs)are involved in various physiological processes,including osteogenesis and angiogenesis.Recent studies have shown that lncRNAs could mediate the crosstalk between angiogenesis and osteogenesis.However,the mechanism by which lncRNAs regulate angiogenesis-osteogenesis crosstalk remains unclear.In this review,we describe in detail the ways in which lncRNAs regulate the crosstalk between osteogenesis and angiogenesis to promote bone health,aiming to provide new directions for the study of the mechanism by which lncRNAs regulate bone metabolism.
基金supported by the National Natural Science Foundation of China,No.81101370,81101399,81272018the Natural Science Foundation of Jiangsu Province in China,No.BK2011303+2 种基金Jiangsu Province Science and Technology Support Program(Social Development)in China,No.BE2011672University Natural Science Research Foundation of Jiangsu Province for Higher Education,No.12KJB320008College Graduate Research and Innovation Plan of Jiangsu Province in China,No.CXZZ11_0126
文摘It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-reperfusion injury in rabbits using somatosensory evoked potential detection technology. The results showed that the somatosensory evoked potential latency was significantly prolonged and the amplitude significantly reduced until it disappeared during the period of spinal cord ischemia. After reperfusion for 30-180 minutes, the amplitude and latency began to gradually recover; at 360 minutes of reperfusion, the latency showed no significant difference compared with the pre-ischemic value, while the somatosensory evoked potential amplitude in- creased, and severe hindlimb motor dysfunctions were detected. Experimental findings suggest that changes in somatosensory evoked potentia~ ~atency can reflect the degree of spinat cord ischemic injury, while the amplitude variations are indicators of the late spinal cord reperfusion injury, which provide evidence for the assessment of limb motor function and avoid iatrogenic spinal cord injury.
文摘AIM:To report the incidence,clinical features and outcomes of gastrointestinal(GI)involvement in Behcet’s disease(BD).METHODS:A total of 168 consecutive patients with BD were screened and upper and lower GI endoscopies were performed in 148 patients.Four hundred age-and sex-matched controls were enrolled for comparison.RESULTS:Fifty-two(35.1%)patients had GI lesions.After a mean follow-up of 10 mo,ileocecal ulcers had been confirmed in 20 patients,including active ulcer(s)in 18 patients,but no ileocecal ulceration was found in controls.GI symptoms were present in 14 patients with active ulcer(s),while 4 patients with smaller ulcer were asymptomatic.Endoscopic features of ileocecalulcer were:a single ulcer(50%),larger than 1 cm in diameter(72.2%),and round/oval or volcano-type in shape(83.3%).Compared with patients without GI involvement,less ocular lesions,lower levels of albumin,erythrocyte count and hemoglobin,and higher levels of C-reactive protein and erythrocyte sedimentation rate were confirmed in the intestinal BD group.Four patients had esophageal ulcers in the BD group but no case in controls.The other endoscopic findings were similar between the two groups.The prevalence of Helicobacter pylori infection was similar in both groups.Most patients received an immunomodulator and responded well.CONCLUSION:GI lesions commonly occur in Chinese BD patients.The most frequently involved area is the ileocecal region.Esophageal ulcer might be a rare but unique lesion.
基金supported by the National Natural Science Foundation of China (Grant No. 91544231)the State Key Research and Development Program of China (Grant No. 2016YFC0200500)+1 种基金Jiangsu Provincial Collaborative Innovation Center of Climate ChangeJun ZOU was also supported by the Program for Outstanding Ph D Candidates of Nanjing University
文摘Radiative aerosols are known to influence the surface energy budget and hence the evolution of the planetary boundary layer. In this study, we develop a method to estimate the aerosol-induced reduction in the planetary boundary layer height （PBLH） based on two years of ground-based measurements at a site, the Station for Observing Regional Processes of the Earth System （SORPES）, at Nanjing University, China, and radiosonde data from the meteorological station of Nanjing. The observations show that increased aerosol loads lead to a mean decrease of 67.1 W m-2 for downward shortwave radiation （DSR） and a mean increase of 19.2 W m-2 for downward longwave radiation （DLR）, as well as a mean decrease of 9.6 W m-2 for the surface sensible heat flux （SHF） in the daytime. The relative variations of DSR, DLR and SHF are shown as a function of the increment of column mass concentration of particulate matter （PM2.5）. High aerosol loading can significantly increase the atmospheric stability in the planetary boundary layer during both daytime and nighttime. Based on the statistical relationship between SHF and PM2.5 column mass concentrations, the SHF under clean atmospheric conditions （same as the background days） is derived. In this case, the derived SHF, together with observed SHF, are then used to estimate changes in the PBLH related to aerosols. Our results suggest that the PBLH decreases more rapidly with increasing aerosol loading at high aerosol loading. When the daytime mean column mass concentration of PM2.5 reaches 200 mg m-2, the decrease in the PBLH at 1600 LST （local standard time） is about 450 m.
基金Project supported by the National Natural Science Foundation of China(Nos.51775486 and 51521064)the Fundamental Research Funds for the Central Universities of China(No.2017FZA4001)the National Basic Research Program(973 Program)of China(No.2015CB058100)
基金supported by the National Nature Science Foundation in China(No.81170323)Shanghai Key Lab of Human Performance(Shanghai University of Sport)(No.11DZ2261100),Shanghai,China
文摘The traditional Chinese medicine(TCM)theory of traditional Chinese health exercise(TCHE)is analyzed.The effect of meridians and acupoints contained in TCHE is summarized,the treatment methods of tonifying deficiencies and reducing excesses applied in TCHE are discussed,the regulation of Zang-fu organs are emphasized,and the leading role of Qi is introduced.The exercise prescription and proper timing of TCHE are described and reported.TCHEs,such as Baduanjin,Liuzijue,and Tai Chi,possess strong TCM characteristics.
基金This work was supported by the National Natural Science Foundation of China(No.82071762)the Shanghai Key Lab of Human Performance(Shanghai University of Sport)(No.11DZ2261100)the 2021 Capacity Building of Shanghai Universities(No.21010503600),China。
文摘Osteoarthritis(OA),characterized by cartilage degeneration,synovial inflammation,and subchondral bone remodeling,is among the most common musculoskeletal disorders globally in people over 60 years of age.The initiation and progression of OA involves the abnormal metabolism of chondrocytes as an important pathogenic process.Cartilage degeneration features mitochondrial dysfunction as one of the important causative factors of abnormal chondrocyte metabolism.Therefore,maintaining mitochondrial homeostasis is an important strategy to mitigate OA.Mitophagy is a vital process for autophagosomes to target,engulf,and remove damaged and dysfunctional mitochondria,thereby maintaining mitochondrial homeostasis.Cumulative studies have revealed a strong association between mitophagy and OA,suggesting that the regulation of mitophagy may be a novel therapeutic direction for OA.By reviewing the literature on mitophagy and OA published in recent years,this paper elaborates the potential mechanism of mitophagy regulating OA,thus providing a theoretical basis for studies related to mitophagy to develop new treatment options for OA.
基金This work was supported by National Key R&D Program of China(2018YFB2000903)NationalNatural Science Foundation of China under Grant Numbers 51875507 and 51890885,Open Fund of Key Laboratory of Electronic Equipment Structure Design in Xidian University(EESD1905)applied by Author Yangqiao Lin,which support the research,the Fundamental Research Funds for the Central Universities,and Director’s Fund of State Key Laboratory of Fluid Power and Mechatronic Systems.
文摘Developing artificial muscles that can replace biological muscles to accomplish various tasks iswhat we have long been aiming for.Recent advances in flexible materials and 3D printing technology greatly promote the development of artificial muscle technology.A variety of flexible material-based artificial muscles that are driven by different external stimuli,including pressure,voltage,light,magnetism,temperature,etc.,have been developed.Among these,fluid-driven artificial muscles(FAMs),which can convert the power of fluid(gas or liquid)into the force output and displacement of flexible materials,are the most widely used actuation methods for industrial robots,medical instruments,and human-assisted devices due to their simplicity,excellent safety,large actuation force,high energy efficiency,and low cost.Herein,the bio-design,manufacturing,sensing,control,and applications of FAMs are introduced,including conventional pneumatic/hydraulic artificial muscles and several innovative artificial muscles driven by functional fluids.What’s more,the challenges and future directions of FAMs are discussed.
基金This work was supported by the National Natural Science Foundation of China(Nos.81901430 and 81871835)the Guangdong Provincial Natural Science Foundation of China(No.2022A1515010379)+1 种基金the Innovation Project from Department of Education of Guangdong Province(No.2021KTSCX 055)the Shanghai Frontiers Science Research Base of Exercise and Metabolic Health,and the Shanghai Key Laboratory for Human Athletic Ability Development and Support(Shanghai University of Sport)(No.11DZ2261100),China.
文摘Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It is widely recognized that circadian clock genes can regulate bone metabolism.In recent years,the regulation of bone metabolism by non-coding RNAs has become a hotspot of research.MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism,including circadian clock genes.However,research in this field has been conducted only in recent years and the mechanisms involved are not yet well established.Recent studies have focused on how to target circadian clock genes to treat some diseases,such as autoimmune diseases,but few have focused on the co-regulation of circadian clock genes and microRNAs in bone metabolic diseases.Therefore,in this paper we review the progress of research on the co-regulation of bone metabolism by circadian clock genes and microRNAs,aiming to provide new ideas for the prevention and treatment of bone metabolic diseases such as osteoporosis.
基金the National Basic Research Program (973) of China (No. 2006CB705400)the National Natural Science Foundation of China (No. 50575200)
文摘Mathematical models are been proposed to simulate the thermal and metallurgical behaviors of the strip occtLrring on the run-out table （ROT） in a hot strip mill. A variational method is utilized for the discretization of the governing transient conduction-convection equation, with heat transfer coefficients adaptively determined by the actual mill data. To consider the thermal effect of phase transformation during cooling, a constitutive equation for describing austenite decomposition kinetics of steel in air and water cooling zones is coupled with the heat transfer model. As the basic required inputs in the numerical simulations, thermal material properties are experimentally measured for three carbon steels and the least squares method is used to statistically derive regression models for the properties, including specific heat and thermal conductivity. The numerical simulation and experimental results show that the setup accuracy of the temperature prediction system of ROT is effectively improved.
基金supported by the National Natural Science Foundation of China (NSFC-DFG, 31861133016NSFC, 31970564)。
文摘Extensive exotic introgression could significantly enlarge the genetic distance of hybrid parental populations to promote strong heterosis.The goal of this study was to investigate whether genome-wide prediction can support pre-breeding in populations with exotic introgressions.We evaluated seed yield,seed yield related traits and seed quality traits of 363 hybrids of Brassica napus (AACC) derived from two parental populations divergent on massive exotic introgression of related species in three environments.The hybrids presented strong heterosis on seed yield,which was much higher than other investigated traits.Five genomic best linear unbiased prediction models considering the exotic introgression and different marker effects (additive,dominance,and epistatic effects) were constructed to test the prediction ability for different traits of the hybrids.The analysis showed that the trait complexity,exotic introgression,genetic relationship between the training set and testing set,training set size,and environments affected the prediction ability.The models with best prediction ability for different traits varied.However,relatively high prediction ability (e.g.,0.728 for seed yield) was also observed when the simplest models were used,excluding the effects of the special exotic introgression and epistasis effect by5-fold cross validation,which would simplify the prediction for the trait with complex architecture for hybrids with exotic introgression.The results provide novel insights and strategies for genome-wide prediction of hybrids between genetically distinct parent groups with exotic introgressions.
基金supported by the National Natural Science Foundation of China(No.81871835)the Shanghai Frontiers Science Research Base of Exercise and Metabolic Health,and the Shanghai Key Lab of Human Performance(Shanghai University of Sport)(No.11DZ2261100),China.
文摘Bone is a connective tissue that has important functions in the human body.Cells and the extracellular matrix(ECM)are key components of bone and are closely related to bone-related diseases.However,the outcomes of conventional treatments for bone-related diseases are not promising,and hence it is necessary to elucidate the exact regulatory mechanisms of bone-related diseases and identify novel biomarkers for diagnosis and therapy.Circular RNAs(circRNAs)are single-stranded RNAs that form closed circular structures without a 5’cap or 3’tail and polycyclic adenylate tails.Due to their high stability,circRNAs have the potential to be typical biomarkers.Accumulating evidence suggests that circRNAs are involved in bone-related diseases,including osteoarthritis,osteoporosis,osteosarcoma,multiple myeloma,intervertebral disc degeneration,and rheumatoid arthritis.Herein,we summarize the recent research progress on the characteristics and functions of circRNAs,and highlight the regulatory mechanism of circRNAs in bone-related diseases.
基金supported by the National Basic Research Program(973)of China(No.2006CB705400)the National Natural Science Founda-tion of China(No.50705082)the National Natural Science Fund for Distinguished Young Scholar(No.50425518),China
文摘We proposed an optimal design method to expand the bandwidth for the control of large hydraulic Stewart platform.The method is based on generalized natural frequency and takes hydraulic oil into consideration.A Lagrangian formulation which considers the whole leg inertia is presented to obtain the accurate equivalent mass matrix.Using the model,the effect of leg inertia and the influence of design parameters on the generalized natural frequency are investigated.Finally,numerical examples are presented to validate and confirm the efficiency of the mathematical model.The results show that the leg inertia,especially the piston part plays an important role in the dynamics.The optimum diameter ratio of the base to the moving platform is between 2 and 3,and the optimum joint angle ratio of the base to the moving platform is about 1.The smaller joint angles and a longer leg stroke are favorable for raising system frequencies.The system oil should be preprocessed for large platforms with a requirement for good dynamic performance.
基金supported by National Key R&D Program of China(2023YFC2306800)National Natural Science Foundation of China(No.82072280 and No.82272315)Beijing Municipal Natural Science Foundation(No.7212063 and No.7222108).
文摘Naturally occurred precore(PC,G1896A)and/or basal core promoter(BCP,A1762T/G1764A)mutations are prevalent in chronic HBV-infected patients,especially those under HBeAg-negative status.However,the replicative capacity of HBV with PC/BCP mutations remains ambiguous.Herein,meta-analysis showed that,only under HBeAg-negative status,the serum HBV DNA load in patients with PC mutation was 7.41-fold higher than those without the mutation.Both PC mutation alone and BCPþPC mutations promoted HBV replication in cell and hydrodynamic injection mouse models.In human hepatocyte chimeric mouse model,BCPþPC mutations led to elevated replicative capacity and intrahepatic core protein accumulation.Mechanistically,preC RNA harboring PC mutation could serve as mRNA to express core and P proteins,and such pgRNA-like function favored the maintenance of cccDNA pool under HBeAg-negative status.Additionally,BCPþPC mutations induced more extensive and severe human hepatocyte damage as well as activated endoplasmic reticulum stress and TNF signaling pathway in livers of chimeric mice.This study indicates that HBeAg-negative patients should be monitored on HBV mutations regularly and are expected to receive early antiviral treatment to prevent disease progression.
基金Project supported by the National Natural Science Foundation of China （No. 51475415）, the Science Fund for Creative Research Groups of the National Natural Science Foundation of China （No. 51221004）, and the Fundamental Research Funds for the Central Universities, China
文摘The poppet valve is a fundamental component in fluid power systems. Under particular conditions, annoying ＂squeal＂ noises may be generated in hydraulic poppet valves. In the present study, the frequency spectrum of the squeal noise is obtained by analyzing the sampling data from the accelerometer mounted on the valve body. It is found that the flow velocity, pressure, and structural parameters have crucial effects on the properties of squeal noise, especially frequency. Larger valve chamber volume or lower backpressure leads to lower fundamental frequency of the squeal noise. An explanation for the squeal noise, as a result of Helmholtz resonance, is suggested and proved by experimental results.
基金Project supported by the National Natural Science Foundation of China(Nos.51875507,51821093,and U1908228)。
文摘Soft robotics,compared with their rigid counterparts,are able to adapt to uncharted environments,are superior in safe human-robot interactions,and have low cost,owing to the native compliance of the soft materials.However,customized complex structures,as well as the nonlinear and viscoelastic soft materials,pose a great challenge to accurate modeling and control of soft robotics,and impose restrictions on further applications.In this study,a unified modeling strategy is proposed to establish a complete dynamic model of the most widely used pneumatic soft bending actuator.First,a novel empirical nonlinear model with parametric and nonlinear uncertainties is identified to describe the nonlinear behaviors of pneumatic soft bending actuators.Second,an inner pressure dynamic model of a pneumatic soft bending actuator is established by introducing a modified valve flow rate model of the unbalanced pneumatic proportional valves.Third,an adaptive robust controller is designed using a backstepping method to handle and update the nonlinear and uncertain system.Finally,the experimental results of comparative trajectory tracking control indicate the validity of the proposed modeling and control method.
基金supported by the National Natural Science Foundation of China(NSFC,31970564,32000397,32171982)the Fundamental Research Funds for the Central Universities(2662023PY004)。
文摘"Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop improvement.Such synthetic allopolyploids often undergo rapid genomic structural variation(SV).However,how such SV arises,is inherited and fixed,and how it affects important traits,has rarely been comprehensively and quantitively studied in advanced generation synthetic lines.A better understanding of these processes will aid breeders in knowing how to best utilize synthetic allopolyploids in breeding programs.Here,we analyzed three genetic mapping populations(735 DH lines)derived from crosses between advanced synthetic and conventional Brassica napus(rapeseed)lines,using whole-genome sequencing to determine genome composition.We observed high tolerance of large structural variants,particularly toward the telomeres,and preferential selection for balanced homoeologous exchanges(duplication/deletion events between the A and C genomes resulting in retention of gene/chromosome dosage between homoeologous chromosome pairs),including stable events involving whole chromosomes("pseudoeuploidy").Given the experimental design(all three populations shared a common parent),we were able to observe that parental SV was regularly inherited,showed genetic hitchhiking effects on segregation,and was one of the major factors inducing adjacent novel and larger SV.Surprisingly,novel SV occurred at low frequencies with no significant impacts on observed fertility and yield-related traits in the advanced generation synthetic lines.However,incorporating genome-wide SV in linkage mapping explained significantly more genetic variance for traits.Our results provide a framework for detecting and understanding the occurrence and inheritance of genomic SV in breeding programs,and support the use of synthetic parents as an important source of novel trait variation.
