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A scalable approach to Na_(4)Fe_(3)(PO_(4))_(2)P_(2)O_(7)@carbon/expanded graphite as cathode for ultralong-lifespan and low-temperature sodium-ion batteries
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作者 Zheng Li Fangkun Li +9 位作者 Xijun Xu jun zeng Hangyu Zhang Lei Xi Yiwen Wu Linwei Zhao Jiahe Chen jun Liu Yanping Huo Shaomin Ji 《Chinese Chemical Letters》 2025年第10期616-622,共7页
Mixed polyanion phosphate Na_(4)Fe_(3)(PO_(4))_(2)P_(2)O_(7)(NFPP)is regarded as the most promising cathode material for sodium-ion batteries(SIBs),due to its high structural stability and low-cost environmental frien... Mixed polyanion phosphate Na_(4)Fe_(3)(PO_(4))_(2)P_(2)O_(7)(NFPP)is regarded as the most promising cathode material for sodium-ion batteries(SIBs),due to its high structural stability and low-cost environmental friendliness.However,its intrinsic low conductivity and sluggish Na^(+)diffusion restricted the fast-charge and low-temperature sodium storage.Herein,an NFPP composite encapsulated by in-situ pyrolytic carbon and coupled with expanded graphite(NFPP@C/EG)was constructed via a sol-gel method followed by a ballmill procedure.Due to the dual-carbon modified strategy,this NFPP@C/EG only enhanced the electronic conductivity,but also endowed more channels for Na^(+)diffusion.As cathode for SIBs,the optimized NFPP(M-NFPP@C/EG)delivers excellent rate capability(capacity of~80.5 mAh/g at 50 C)and outstanding cycling stability(11000 cycles at 50 C with capacity retention of 89.85%).Additionally,cyclic voltammetry(CV)confirmed that its sodium storage behavior is pseudocapacitance-controlled,with in-situ electrochemical impedance spectroscopy(EIS)further elucidating improvements in electrode reaction kinetics.At lower temperatures(0℃),M-NFPP@C/EG demonstrated exceptional cycling performance(8800 cycles at 10 C with capacity retention of 95.81%).Moreover,pouch cells also exhibited excellent stability.This research demonstrates the feasibility of a dual carbon modification strategy in enhancing NFPP and proposes a low-cost,high-rate,and ultra-stable cathode material for SIBs. 展开更多
关键词 Na_(4)Fe_(3)(PO_(4))_(2)P_(2)O_(7) Expanded graphite Dual-carbon modified Polyanionic compounds cathode Sodium-ion batteries
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Robust measurement of orbital angular momentum of a partially coherent vortex beam under amplitude and phase perturbations 被引量:1
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作者 Zhao Zhang Gaoyuan Li +5 位作者 Yonglei Liu Haiyun Wang Bernhard J.Hoenders Chunhao Liang Yangjian Cai jun zeng 《Opto-Electronic Science》 2024年第1期1-12,共12页
The ability to overcome the negative effects,induced by obstacles and turbulent atmosphere,is a core challenge of long-distance information transmission,and it is of great significance in free-space optical communicat... The ability to overcome the negative effects,induced by obstacles and turbulent atmosphere,is a core challenge of long-distance information transmission,and it is of great significance in free-space optical communication.The spatial-coherence structure,that characterizes partially coherent fields,provides a new degree of freedom for carrying information.However,due to the influence of the complex transmission environment,the spatial-coherence structure is severely damaged during the propagation path,which undoubtedly limits its ability to transmit information.Here,we realize the robust far-field orbital angular momentum(OAM)transmission and detection by modulating the spatial-coherence structure of a partially coherent vortex beam with the help of the cross-phase.The cross-phase enables the OAM information,quantified by the topological charge,hidden in the spatial-coherence structure can be stably transmitted to the far field and can resist the influence of obstructions and turbulence within the communication link.This is due to the self-reconstruction property of the spatial-coherence structure embedded with the cross-phase.We demonstrate experimentally that the topological charge information can be recognized well by measuring the spatial-coherence structure in the far field,exhibiting a set of distinct and separated dark rings even under amplitude and phase perturbations.Our findings open a door for robust optical signal transmission through the complex environment and may find application in optical communication through a turbulent atmosphere. 展开更多
关键词 degree of coherence orbital angular momentum cross-phase topological charge information transmission
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Deep learning-assisted common temperature measurement based on visible light imaging
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作者 朱佳仪 何志民 +8 位作者 黄成 曾峻 林惠川 陈福昌 余超群 李燕 张永涛 陈焕庭 蒲继雄 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第8期230-236,共7页
Real-time,contact-free temperature monitoring of low to medium range(30℃-150℃)has been extensively used in industry and agriculture,which is usually realized by costly infrared temperature detection methods.This pap... Real-time,contact-free temperature monitoring of low to medium range(30℃-150℃)has been extensively used in industry and agriculture,which is usually realized by costly infrared temperature detection methods.This paper proposes an alternative approach of extracting temperature information in real time from the visible light images of the monitoring target using a convolutional neural network(CNN).A mean-square error of<1.119℃was reached in the temperature measurements of low to medium range using the CNN and the visible light images.Imaging angle and imaging distance do not affect the temperature detection using visible optical images by the CNN.Moreover,the CNN has a certain illuminance generalization ability capable of detection temperature information from the images which were collected under different illuminance and were not used for training.Compared to the conventional machine learning algorithms mentioned in the recent literatures,this real-time,contact-free temperature measurement approach that does not require any further image processing operations facilitates temperature monitoring applications in the industrial and civil fields. 展开更多
关键词 convolutional neural network visible light image temperature measurement low-to-medium-range temperatures
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间歇性外斜视术后中远期眼位的相关因素分析 被引量:7
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作者 曾俊 刘陇黔 《国际眼科杂志》 CAS 北大核心 2020年第8期1448-1451,共4页
目的:探讨间歇性外斜视患者术后中远期眼位的影响因素。方法:回顾性分析我院眼科2017-01/2018-08收治的间歇性外斜视患者78例的临床资料,收集患者的斜视类型、手术年龄、术前有无近立体视和斜视度及术后第1d斜视度等资料,探讨术后中远... 目的:探讨间歇性外斜视患者术后中远期眼位的影响因素。方法:回顾性分析我院眼科2017-01/2018-08收治的间歇性外斜视患者78例的临床资料,收集患者的斜视类型、手术年龄、术前有无近立体视和斜视度及术后第1d斜视度等资料,探讨术后中远期眼位的影响因素。结果:本研究纳入患者术后中远期眼位正位者47例(60%),其中术前集合不足型患者43例,术后中远期眼位正位者18例(42%);基本型患者31例,术后中远期眼位正位者26例(84%);分开过强型患者4例,术后中远期眼位正位者3例(75%)。单因素分析结果显示,术后中远期眼位正位者与无效者斜视类型、术前视近斜视度具有差异(P<0.05);多因素Logistic回归分析显示,斜视类型(基本型)是影响间歇性外斜视患者术后中远期眼位的危险因素(OR=5.769,95%CI:1.790~18.595,P<0.05)。结论:斜视类型是间歇性外斜视患者术后中远期眼位的独立影响因素,临床应引起足够重视。 展开更多
关键词 间歇性外斜视 术后眼位 斜视类型 斜视度 影响因素
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Beyond the desert sands:decoding the relationship between camels,gut microbiota,and antibiotic resistance through metagenomics
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作者 Yan Gao Jiangchao Wu +2 位作者 jun zeng Xiangdong Huo Kai Lou 《Science in One Health》 2024年第1期158-170,共13页
Background:Camels,known as the enduring“ships of the desert,”host a complex gut microbiota that plays a crucial role in their survival in extreme environments.However,amidst the fascinating discoveries about the cam... Background:Camels,known as the enduring“ships of the desert,”host a complex gut microbiota that plays a crucial role in their survival in extreme environments.However,amidst the fascinating discoveries about the camel gut microbiota,concerns about antibiotic resistance have emerged as a significant global challenge affecting both human and animal populations.Indeed,the continued use of antibiotics in veterinary medicine has led to the widespread emergence of antibiotic-resistant bacteria,which has worsened through gene transfer.Methods:This study offers a deeper examination of this pressing issue by harnessing the potent tools of metagenomics to explore the intricate interplay between the camel(Camelus ferus)gut microbiota and antibiotic resistance.Results:Samples from wild camels yielded varying amounts of raw and clean data,generating scaftigs and open reading frames.The camel fecal microbiome was dominated by bacteria(mainly Bacillota and Bacteriodota),followed by viruses,archaea,and eukaryota.The most abundant genera were the Bacteroides,Ruminococcus,and Clostridium.Functional annotation revealed enriched pathways in metabolism,genetic information processing,and cellular processes,with key pathways involving carbohydrate transport and metabolism,replication,and amino acid transport.CAZy database analysis showed high abundances of glycoside hydrolases and glycosyl transferases.Antibiotic resistance gene(ARG)analysis identified Bacillota and Bacteroidota as the main reservoirs,with vancomycin resistance genes being the most prevalent.This study identified three major resistance mechanisms:antibiotic target alteration,antibiotic target protection,and antibiotic efflux.Conclusion:These findings contribute to a broader understanding of antibiotic resistance within animal microbiomes and provide a foundation for further investigations of strategies to manage and mitigate antibiotic resistance. 展开更多
关键词 METAGENOMICS CAMELS Gut microbiota Antibiotic resistance
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高管强制变更、分析师跟踪与企业创新投入 被引量:15
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作者 曾军 董博 陈红 《会计与经济研究》 CSSCI 北大核心 2020年第1期38-51,共14页
文章以2010-2017年中国沪深两市A股上市公司为样本,实证考察了高管强制变更对企业创新投入的影响以及分析师跟踪的调节作用。研究发现,高管强制变更与企业创新投入呈显著负相关关系,分析师跟踪可以减少企业高管短视行为,提升企业高管团... 文章以2010-2017年中国沪深两市A股上市公司为样本,实证考察了高管强制变更对企业创新投入的影响以及分析师跟踪的调节作用。研究发现,高管强制变更与企业创新投入呈显著负相关关系,分析师跟踪可以减少企业高管短视行为,提升企业高管团队的稳定性,从而缓解高管强制变更对企业创新投入的抑制作用。进一步研究发现,盈余信息质量和产权性质会影响分析师跟踪调节作用的发挥,从而证实分析师通过缓解企业信息不对称程度和委托代理问题来发挥其调节功能。 展开更多
关键词 高管强制变更 分析师跟踪 企业创新投入 产权性质 信息不对称
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基于深度学习的溃坝涌波爬高过程研究 被引量:3
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作者 陈家祺 曾俊 刘海江 《水动力学研究与进展(A辑)》 CSCD 北大核心 2022年第3期426-431,共6页
该文将卷积神经网络应用于实验室内的溃坝涌波爬高图像数据集,通过对比FCN和U-Net两类语义分割网络,遴选出U-Net作为深度学习模型展开应用。结果表明,U-Net模型在溃坝涌波爬高识别任务中能达到令人满意的效果,但其迁移能力还有待进一步... 该文将卷积神经网络应用于实验室内的溃坝涌波爬高图像数据集,通过对比FCN和U-Net两类语义分割网络,遴选出U-Net作为深度学习模型展开应用。结果表明,U-Net模型在溃坝涌波爬高识别任务中能达到令人满意的效果,但其迁移能力还有待进一步提升。由于该模型对湿润床面上所记录图像中干扰信号的抵抗能力较弱,故对于未参与模型训练的溃坝涌波水体回流图像的识别效果较差。此外,该文还对模型深度学习过程中的三个超参数(学习率、批尺寸和卷积核尺寸)开展了敏感性分析,揭示了这三个超参数对模型预测结果的影响,为后续研究提供了相关参考。 展开更多
关键词 物理试验 卷积神经网络 溃坝涌波 爬高 超参数
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Effect of organic wastes on the plant-microbe remediation for removal of aged PAHs in soils 被引量:13
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作者 Jing Zhang Xiangui Lin +3 位作者 Weiwei Liu Yiming Wang jun zeng Hong Chen 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第8期1476-1482,共7页
The effectiveness of in-situ bioremediation of polycyclic aromatic hydrocarbons (PAHs) may be inhibited by low nutrients and organic carbon. To evaluate the effect of organic wastes on the PAHs removal efficiency of... The effectiveness of in-situ bioremediation of polycyclic aromatic hydrocarbons (PAHs) may be inhibited by low nutrients and organic carbon. To evaluate the effect of organic wastes on the PAHs removal efficiency of a plant-microbe remediation system, contaminated agricultural soils were amended with different dosages of sewage sludge (SS) and cattle manure (CM) in the presence of alfalfa (Medicago sativa L.) and PAHs-degraders (Bacillus sp. and Flavobacterium sp.). The results indicated that the alfalfa mean biomasses varied from 0.56 to 2.23 g/pot in root dry weight and from 1.80 to 4.88 g/pot in shoot dry weight. Low dose amendments, with rates of SS at 0.1% and CM at 1%, had prominent effects on plant growth and soil PAHs degradation. After 60-day incubation, compared with about 5.6% in the control, 25.8% PAHs removal was observed for treatments in the presence of alfalfa and PAHs-degraders; furthermore, when amended with different dosages of SS and CM, the removed PAHs from soils increased by 35.5%--44.9% and 25.5%-42.3%, respectively. In particular, the degradation of high-molecular-weight PAHs was up to 42.4%. Dehydrogenase activities (DH) ranged between 0.41 and 1.83 ~tg triphenylformazan/(g dry soil.hr) and the numbers of PAHs-degrading microbes (PDM) ranged from 1.14x106 to 16.6x106 most-probable-number/g dry soil. Further investigation of the underlying microbial mechanism revealed that both DH and PDM were stimulated by the addition of organic wastes and significantly correlated with the removal ratio of PAHs. In conclusion, the effect of organic waste application on soil PAHs removal to a great extent is dependent on the interactional effect of nutrients and dissolved organic matter in organic waste and soil microorganisms. 展开更多
关键词 polycyclic aromatic hydrocarbons (PAHs) soil contamination BIOREMEDIATION organic wastes
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Clinicopathological significance of overexpression of interleukin-6 in colorectal cancer 被引量:11
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作者 jun zeng Zhong-Hua Tang +1 位作者 Shuang Liu Shan-Shan Guo 《World Journal of Gastroenterology》 SCIE CAS 2017年第10期1780-1786,共7页
AIM To compare the expression levels of interleukin(IL)-6 in colorectal cancer(CRC) tissues and adjacent noncancerous tissues, and analyse the correlation of IL-6 expression with the clinicopathological parameters of ... AIM To compare the expression levels of interleukin(IL)-6 in colorectal cancer(CRC) tissues and adjacent noncancerous tissues, and analyse the correlation of IL-6 expression with the clinicopathological parameters of CRC. METHODS Fifty CRC tissue specimens and 50 matched adjacent mucosa specimens were collected. The expression of IL-6 in these clinical samples was examined by immunohistochemical staining. The correlation between IL-6 expression and clinicopathological parameters was assessed by statistical analysis.RESULTS IL-6 expression was significantly elevated in CRC tissues compared with noncancerous tissues(P < 0.001). IL-6 expression was positively correlated with tumour TNM stage(P < 0.001), but a negative correlation was detected between IL-6 expression and tumor histological differentiation in CRC(P < 0.05). Furthermore, IL-6 expression was associated with invasion depth and lymph node metastasis in CRC. CONCLUSION IL-6 might be a useful marker for predicting a poor prognosis in patients with CRC and might be used as a potential therapeutic target in CRC. 展开更多
关键词 Colorectal cancer INTERLEUKIN-6 Invasion depth Lymph node metastases
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Comparative Analysis of Leaf Trichomes, Epidermal Wax And Defense Enzymes Activities in Response to Puccinia horiana in Chrysanthemum and Ajania Species 被引量:10
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作者 Yinjie Wang jun zeng +9 位作者 Xiaolong Xia Yang Xu Jing Sun Jing Gu Hainan Sun Haini Lei Fadi Chen Jiafu Jiang Weiming Fang Sumei Chen 《Horticultural Plant Journal》 SCIE 2020年第3期191-198,共8页
White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants.To better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defe... White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants.To better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defensive enzymes activities of immune(Chrysanthemum makinoi var.wakasaense)and highly susceptible(Ajania shiwogiku var.kinokuniense)species were compared.Trichome density of two species was markedly different,negatively associated with plant resistance to P.horiana.Total wax load in C.makinoi var.wakasaense was two times more than that in A.shiwogiku var.kinokuniense.The wax composition in immune one was abundant in esters and primary alcohols.Superoxide dismutase(SOD,EC 1.15.1.1),peroxidase(POD,EC 1.11.1.7),polyphenoloxidase(PPO,EC 1.14.18.1 or EC 1.10.3.2)and phenylalanine ammonia lyase(PAL,EC 4.3.1.5)activitieswere investigated.In C.makinoi var.wakasaense,the activity of SOD and POD increased rapidly after inoculation,whichmight be non-host induced reactive oxygen species(ROS)activated antioxidant enzymes,however SOD and POD remained a low and steady level in the highly susceptible one after inoculation.Quick increase in PPO activities after inoculation was observed in both species,however it remained higher in C.makinoi var.wakasaense at the late period of inoculation.PAL in C.makinoi var.wakasaense was induced after pathogen inoculation,but not in A.shiwogiku var.kinokuniense,suggesting that these two enzymes might contribute to the resistance to P.horiana. 展开更多
关键词 CHRYSANTHEMUM Puccinia horiana Resistance Leaf cuticular wax Enzyme activity
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The macular microstructure repair and predictive factors of surgical outcomes after vitrectomy for idiopathic macular hole 被引量:4
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作者 Jing-Ling Zou jun zeng 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2019年第5期852-857,共6页
To evaluate the macular microstructure repair and explore the factors related to those changes and visual improvement after vitrectomy for idiopathic macular hole(IMH). Totally 19 eyes of 18 IMH patients who underwent... To evaluate the macular microstructure repair and explore the factors related to those changes and visual improvement after vitrectomy for idiopathic macular hole(IMH). Totally 19 eyes of 18 IMH patients who underwent macular hole(MH) surgery were evaluated with bestcorrected visual acuity(BCVA) and spectral-domain optical coherence tomography(SD-OCT) images. All 19 eyes closed at 6 mo postoperatively. BCVA was observed gradually improved(P<0.001), with subretinal fluid(SRF) gradually absorbed(P=0.021) and the rate of external limiting membrane(ELM) defects gradually decreased(P=0.011) with follow-up time. Poorer postoperative logMAR BCVA correlated with larger MH minimum diameter(P<0.001), larger MH basal diameter(P=0.008), longer symptom duration(P=0.002) and poorer preoperative logMAR BCVA(P=0.010). More improvement in BCVA correlated only with poorer preoperative in logMAR BCVA(P=0.002). The earlier reconstruction of ELM was associated with smaller MH basal diameter(P=0.022) and shorter symptom duration(P=0.008). In conclusion, smaller basal diameter of MH and shorter symptom duration were key factors in earlier reconstruction of ELM. 展开更多
关键词 IDIOPATHIC MACULAR HOLE MACULAR MICROSTRUCTURE PREDICTIVE factors
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Core-shell lipid-polymer nanoparticles as a promising ocular drug delivery system to treat glaucoma 被引量:3
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作者 Yang Zhou Aiping Fang +7 位作者 Fazhan Wang Huili Li Quansheng Jin Lingjing Huang Chunmei Fu jun zeng Zhaohui Jin Xiangrong Song 《Chinese Chemical Letters》 SCIE CAS CSCD 2020年第2期494-500,共7页
Nowadays,tremendous researches have been focused on the core-shell lipid-polymer nanoparticles(LPNs) due to the advantages of both liposomes and polymer nanoparticles.In this work,LPNs were applied to encapsulate brin... Nowadays,tremendous researches have been focused on the core-shell lipid-polymer nanoparticles(LPNs) due to the advantages of both liposomes and polymer nanoparticles.In this work,LPNs were applied to encapsulate brinzolamide(Brz-LPNs) for achieving sustained drug release,improving drug corneal permeation and enhancing drug topical therapeutic effect.The structure of Brz-LPNs was composed of poly(lactic-co-glycolic) acid(PLGA) nanocore which encapsulated Brz(Brz-NPs) and lipid shell around the core.Brz-LPNs were prepared by a modified thin-film dispersion method.With the parameters optimization of Brz-LPNs,optimal Brz-LPNs showed an average particle size of151.23±1.64 nm with a high encapsulation efficiency(EE) of 86.7%±2.28%.The core-shell structure of Brz-LPNs were confirmed by transmission electronic microscopy(TEM).Fourier transformed infrared spectra(FTIR) analysis proved that Brz was successfully entrapped into Brz-LPNs.Brz-LPNs exhibited obvious sustained release of Brz,compared with AZOPT^■ and Brz-LPs.Furthermore,the corneal accumulative permeability of Brz-LPNs significantly increased compared to the commercial available formulation(AZOPT^■) in vitro.Moreover,Brz-LPNs(1 mg/mL Brz) showed a more sustained and effective intraocular pressure(IOP) reduction than Brz-LPs(1 mg/mL) and AZOPT^■(10 mg/mL Brz) in vivo.In conclusion,Brz-LPNs,as promising ocular drug delivery systems,are well worth developing in the future for glaucoma treatment. 展开更多
关键词 CORE-SHELL lipid-polymer nanoparticles OCULAR drug delivery system Sustained release CORNEAL PERMEATION INTRAOCULAR pressure
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Clinical study on Hypotony following blunt ocular trauma 被引量:2
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作者 Chun Ding jun zeng 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2012年第6期771-773,共3页
AIM: To evaluate the incidence and risk factors of hypotony in patients with blunt ocular trauma. METHODS: The medical records of 145 patients with blunt ocular trauma were reviewed. Hypotony was defined as an average... AIM: To evaluate the incidence and risk factors of hypotony in patients with blunt ocular trauma. METHODS: The medical records of 145 patients with blunt ocular trauma were reviewed. Hypotony was defined as an average intraocular pressure (IOP) of 5mmHg or less for three times. RESULTS: Among these 145 patients, hypotony was noted in 10 (6.9%) patients. The rate of hypotony in patients with ciliochoroidal detachment was 66.7% (2 out of 3 eyes), and 5.6% (8 out of 142 eyes) in patients without ciliochoroidal detachment,the difference was statistically significant (P=0.003). The rate of hypotony in patients with traumatic retinal detachment was 18.5% (5 out of 27 eyes), and 4.2% (5 out of 118 eyes) in patients without traumatic retinal detachment, the difference was statistically significant (P=0.026). The rate of hypotony in the patients with anterior proliferative vitreoretinopathy was 42.9% (3 out of 7 eyes) and 5.1% (7 out of 138 eyes) in the patients without anterior proliferative vitreoretinopathy, the difference was statistically significant(P=0.002). CONCLUSION: Ocular hypotension is a complication of blunt ocular trauma. The risk factors include ciliochoroidal detachment, traumatic retinal detachment, and anterior proliferative vitreoretinopathy. 展开更多
关键词 HYPOTONY blunt trauma anterior proliferative vitreoretinopathy ciliochoroidal detachment traumatic retinal detachment
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Identification and Characterization of a δ-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota 被引量:2
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作者 Hui Zhou Yan-Long Yang +3 位作者 jun zeng Ling Zhang Zhi-Hui Ding Ying zeng 《Natural Products and Bioprospecting》 CAS 2016年第3期167-171,共5页
Sesquiterpenoids are very common among natural products.A large number of sesquiterpene synthase genes have been cloned and functionally characterized.However,until now there is no report about the d-cadinol synthase ... Sesquiterpenoids are very common among natural products.A large number of sesquiterpene synthase genes have been cloned and functionally characterized.However,until now there is no report about the d-cadinol synthase predominantly forming d-cadinol(syn.torreyol)from farnesyl diphosphate.Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans.This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B.vibrans.Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B.vibrans.The crude and purified recombinant enzymes,when incubating with farnesyl diphosphate as substrate,gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase.Graphical Abstract A new sesquiterpene synthase gene was cloned from the basidiomycete fungus Boreostereum vibrans and heterologously expressed in E.coli.The purified recombinant enzyme gave δ-cadinol as its principal product from farnesyl diphosphate and thereby identified as a δ-cadinol synthase(BvCS). 展开更多
关键词 Delta-cadinol Sesquiterpene synthase BIOSYNTHESIS GC-MS FUNGI
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Mendelian randomization study to investigate the causal relationship between plasma homocysteine and chronic obstructive pulmonary disease 被引量:2
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作者 Yanlan Hu Ping Tan +8 位作者 juntao Wang jun zeng Quan Li Shijiao Yan Wenjie Hao Lanfen He Xingyue Song Caihong Zhang Chuanzhu Lyu 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2023年第5期367-371,共5页
BACKGROUND:Several observational studies have shown an association between homocysteine(Hcy)levels and chronic obstructive pulmonary disease(COPD),but causal relationships are not clear.Our study aimed to explore the ... BACKGROUND:Several observational studies have shown an association between homocysteine(Hcy)levels and chronic obstructive pulmonary disease(COPD),but causal relationships are not clear.Our study aimed to explore the causal relationship between plasma Hcy and COPD by two-sample Mendelian randomization(MR).METHODS:A two-sample MR study was performed to infer the causal link.Genetically predicted plasma Hcy was selected as an instrumental variable(Ⅳ)from published genome-wide association study(GWAS)meta-analyses.COPD with different etiologies was extracted as outcome variables from other GWAS meta-analyses.The main MR analysis was performed using the inversevariance weighted(IVW)method.Additional analyses were further performed using Cochran’s Q-test and MR-Egger regression to evaluate the heterogeneity or horizontal pleiotropy of our findings.RESULTS:MR analysis showed no significant association between plasma Hcy and COPD.The results of the groups were consistent with the sensitivity analysis and repeated analysis,without heterogeneity or horizontal pleiotropy.The IVW results showed COPD hospital admissions(odds ratio[OR]1.06,95%confidence interval[CI]0.91-1.24,P=0.42),asthma/COPD(OR 0.97,95%CI0.89-1.06,P=0.55),COPD-related chronic infection(OR 1.50,95%CI 0.57-3.99,P=0.41),COPDI asthma/interstitial lung disease(ILD)-related pneumonia or pneumonia-derived septicemia(OR 0.93,95%CI 0.86-1.02,P=0.13),and COPD-related respiratory insufficiency(OR 1.00,95%CI 0.7-1.44,P=0.99).CONCLUSION:There is no direct causal relationship between plasma Hcy and COPD in our study.As Hcy is known to have deleterious effects on endothelial function and vascular homeostasis,further studies are needed to investigate whether additional factors mediate the association between Hcy and COPD. 展开更多
关键词 HOMOCYSTEINE Chronic obstructive pulmonary disease Mendelian randomization
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Knobloch syndrome caused by homozygous frameshift mutation of the COL18A1 gene in a Chinese pedigree 被引量:2
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作者 Lu-Si Zhang Hai-Bo Li +2 位作者 jun zeng Yan Yang Chun Ding 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第6期918-922,共5页
AIM: To explore the clinical feature and genetic etiology of a Chinese Knobloch syndrome family. METHODS: Ocular examinations and magnetic resonance imagings (MRIs) were performed on the family. Whole exome sequen... AIM: To explore the clinical feature and genetic etiology of a Chinese Knobloch syndrome family. METHODS: Ocular examinations and magnetic resonance imagings (MRIs) were performed on the family. Whole exome sequencing was conducted on the two patients. Sanger sequencing was utilized to validate the presence of variation in the family as well as in 100 normal controls. Real-time uantitative polymerase chain reaction (PCR) was used to detect the expression level of COL18A1 in peripheral blood lymphocytes of the patients and normal carriers. RESULTS: The affected subjects presented with vision loss, exotropia, cataracts, retinal detachment, and other complications. A homozygous c.4759_4760delCT (p.Leu1587ValfsX72) mutation (rs398122391) in COL18A1 was identified in the two patients, cosegregating with the phenotypes, and did not be detected in 100 normal controls. This mutation caused significant decreased expression of COL18A1 mRNA in the patients. CONCLUSION: The findings strongly indicate that this mutation is the disease-causing mutation. Moreover, this is the first Knobloch syndrome pedigree reported in the Chinese population. 展开更多
关键词 Knobloch syndrome COL18A1 whole exomesequencing
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Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus(Coccolithoviruses)serine palmitoyltransferase(SPT)gene by electroporation 被引量:1
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作者 Weicong CAI Xueting WANG +4 位作者 Jinjing SU Jian LI jun zeng Guiling LI Jingwen LIU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2021年第2期693-704,共12页
Emiliania huxleyi is the most prominent modern coccolithophore,a group of marine unicellular eukaryotes that play a critical role in ocean biogeochemistry.Coccolithoviruses are large double stranded DNA viruses,which ... Emiliania huxleyi is the most prominent modern coccolithophore,a group of marine unicellular eukaryotes that play a critical role in ocean biogeochemistry.Coccolithoviruses are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed by E.huxleyi.E.huxleyi virus(EhVs)acquired a series of enzyme-coding genes predicted to be involved in the sphingolipid biosynthesis by horizontal gene transfer between virus-host.Currently,there is limited experimental validation identifying the functions of these genes in EhV.Genetic transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied organisms.Serine palmitoyltransferase(SPT)catalyzes the first committed step in de novo sphingolipid biosynthetic pathway.Here,a novel vector system for the transformation of E.huxleyi was designed.It contained fragments of promoter and terminator sequences of E.huxleyi endogenic fucoxanthin chlorophyll a/c-binding protein gene“fcp”and harbored EhV-99B1 spt gene.The resultant recombinant transformation vectors pEhux-I-spt and pEhux-II were co-transferred into E.huxleyi BOF92 by electroporation.Transformants were obtained upon glufosinate-ammonium selection,and confirmed by Southern hybridization,genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein levels.The expression of the viral spt gene led to differences in lipid compositions analyzed using thin-layer chromatography(TLC).The results present the genetic transformation system for E.huxleyi,providing additional genetic resource with potential for exploring basic biological questions such as the virus-host interactions. 展开更多
关键词 E miliania huxleyi coccolithovirus genetic transformation serine palmitoyltransferase(SPT) total lipid
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Effects of Space Radiation Mutation on Germination and Growth of Sugarcane Hybrid Seeds 被引量:3
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作者 Yimei GAN Yuanli WU +4 位作者 jun zeng Wenwei CAI Zhengying CAO Lishun PENG Benpeng YANG 《Agricultural Biotechnology》 CAS 2019年第6期15-18,共4页
[Objectives] This study was conducted to investigate the mutagenic effects of space radiation mutagenesis on sugarcane hybrid seeds. [Methods] Sugarcane hybrid spikes were subjected to space radiation mutagenesis trea... [Objectives] This study was conducted to investigate the mutagenic effects of space radiation mutagenesis on sugarcane hybrid seeds. [Methods] Sugarcane hybrid spikes were subjected to space radiation mutagenesis treatment to identify the effects on the germination, seedling growth and plant growth and development of sugarcane hybrid seeds. [Results] The space radiation treatment had a great impact on the germination and growth of sugarcane hybrid seeds, especially on the germination of sugarcane seeds. The germination number per gram of seeds increased significantly, and the survival rate of seedlings increased significantly. The effect on the growth status was relatively small. The average number of tillers decreased slightly, the percentage of available stems increased, and the plants became higher, which resulted in a greater effect on sugarcane yield. Sugarcane sucrose was increased. The seed germination and late growth status indicated that space radiation promoted the germination and growth of sugarcane hybrid seeds. [Conclusions] This study provides a basis for the space radiation mutagenesis breeding of sugarcane hybrid seeds and the promotion of sugarcane breeding process. 展开更多
关键词 Space radiation SUGARCANE Mutation breeding Seed germination
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A voltammetric sensor for simultaneous determination of lead,cadmium and zinc on an activated carbon fiber rod 被引量:1
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作者 Wei-Jie Wang Yan-Ling Cai +3 位作者 Bai-Chuan Li jun zeng Zhi-Yong Huang Xiao-Mei Chen 《Chinese Chemical Letters》 SCIE CAS CSCD 2018年第1期111-114,共4页
In this work, a simple, low cost and sensitive voltammetric sensor was developed for the simultaneous detection of lead (Pb2+), cadmium (Cd2+), and zinc (Zn2+) ions based on a disposable carbon fiber rod (CF... In this work, a simple, low cost and sensitive voltammetric sensor was developed for the simultaneous detection of lead (Pb2+), cadmium (Cd2+), and zinc (Zn2+) ions based on a disposable carbon fiber rod (CFR). The important factors to enhance the sensing property were creation of a clean surface by dealing with CFR at a high potential and electrochemical deposition of bismuth (Bi) film to improve the accumulation of heavy metal ions. The morphology and conductivity of such activated CFR was characterized by scanning electron microscopy and electrochemical impedance spectroscopy, respectively. In terms of application, differential pulse anodic stripping voltammetry (DPASV) was employed for the simultaneous detection of Pb2+, Cd2+, and Zn2+ on Bi film-coated activated CFR. Experimental parameters, such as the pH value of buffer solution, stirring speed and enrichment factors were optimized. Under optimal conditions, the DPASV peak currents showed good linear relationships with Pb2+, Cd2+ and Zn2+ concentrations in the range of 0.5-2.25 μg/L, 0.5-4.0 μg/L and 1.0- 4.0 μg/L with detection limits of 0.1, 0.3 and 1.0 μg/L (SIN = 3), respectively. Finally, the proposed analysis system was successfully utilized for the simultaneous detection of Pb2+, Cd2+, and Zn2+ contents in rice samples. This study indicated that Bi film-coated activated CFR based DPASV sensor can be a promising and reliable tool for rapid analysis of emergency Dollution affairs of heavv metal ions in food. 展开更多
关键词 Carbon fiber rodBismuth filmDifferential pulse anodic stripping voltammetryHeavy metal ionsSensor
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Measurement of optical coherence structures of random optical fields using generalized Arago spot experiment 被引量:1
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作者 Xin Liu Qian Chen +2 位作者 jun zeng Yangjian Cai Chunhao Liang 《Opto-Electronic Science》 2023年第2期27-35,共9页
The optical coherence structures of random optical fields can determine beam propagation behavior,light–matter interactions,etc.Their performance makes a light beam robust against turbulence,scattering,and distortion... The optical coherence structures of random optical fields can determine beam propagation behavior,light–matter interactions,etc.Their performance makes a light beam robust against turbulence,scattering,and distortion.Recently,we proposed optical coherence encryption and robust far-field optical imaging techniques.All related applications place a high demand on precision in the experimental measurements of complex optical coherence structures,including their real and imaginary parts.Past studies on these measurements have mainly adopted theoretical mathematical approximations,limited to Gaussian statistic involving speckle statistic(time-consuming),or used complicated and delicate optical systems in the laboratory.In this study,we provide:a robust,convenient,and fast protocol to measure the optical coherence structures of random optical fields via generalized Arago(or Poisson)spot experiments with rigorous mathematical solutions.Our proposal only requires to capture the intensity thrice,and is applicable to any optical coherence structures,regardless of their type or optical statistics.The theoretical and experimental results demonstrated that the real and imaginary parts of the structures could be simultaneously recovered with high precision.We believe that such a protocol can be widely employed in phase measurement,optical imaging,and image transfer. 展开更多
关键词 optical coherence statistical optics Arago spot optical encryption optical imaging
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