BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling p...BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal cells.Moreover,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal cells.Cajal cells are the pacemakers of gastrointestinal motility.AIM To investigate the effects of Bifidobacterium lactis(B.lactis)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit pathway.METHODS The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 days.The tail group was given B.lactis BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/day.Hematoxylin&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and PCR.The fecal flora and mucosal flora of rats in each group were detected by 16S rRNA.RESULTS Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic intervention.Simulated microgravity reduced the expressions of SCF and c-kit,and increased the expressions of ERK and P-ERK in the gastric antrum of rats.However,after probiotic intervention,the expressions of SCF and ckit were increased,while the expressions of ERK and P-ERK were decreased,with statistical significance(P<0.05).In addition,simulated microgravity can reduce the operational taxonomic unit(OTU)of the overall intestinal flora of rats,B.lactis BLa80 can increase the OTU of rats,simulated microgravity can reduce the overall richness and diversity of stool flora of rats,increase the abundance of firmicutes in stool flora of rats,and reduce the abundance of Bacteroides in stool flora of rats,most of which are mainly beneficial bacteria.Simulated microgravity can increase the overall richness and diversity of mucosal flora,increase the abundance of Bacteroides and Desulphurides in the rat mucosal flora,and decrease the abundance of firmicutes,most of which are proteobacteria.After probiotics intervention,the overall Bacteroidetes trend in simulated microgravity rats was increased.CONCLUSION B.lactis BLa80 can ameliorate intestinal mucosal injury,regulate intestinal flora,inhibit ERK expression,and activate the SCF/c-kit signaling pathway,which may have a facilitating effect on gastrointestinal motility in simulated microgravity rats.展开更多
In response to the capabilities presented by the High-Intensity Heavy Ion Accelerator Facility(HIAF) and the Accelerator-Driven Subcritical System(Ci ADS), as well as the proposed Chinese Advanced Nuclear Physics Rese...In response to the capabilities presented by the High-Intensity Heavy Ion Accelerator Facility(HIAF) and the Accelerator-Driven Subcritical System(Ci ADS), as well as the proposed Chinese Advanced Nuclear Physics Research Facility(CNUF), we are assembling a consortium of experts in relevant disciplines, both domestically and internationally,to delineate high-precision physics experiments that leverage the state-of-the-art research environment afforded by CNUF.Our focus encompasses six primary domains of inquiry: hadron physics—including endeavors such as the super eta factory and investigations into light hadron structures;muon physics;neutrino physics;neutron physics;the testing of fundamental symmetries;and the exploration of quantum effects within nuclear physics, along with the utilization of vortex accelerators.We aim to foster a well-rounded portfolio of large, medium, and small-scale projects, thus unlocking new scientific avenues and optimizing the potential of the Huizhou large scientific facility. The aspiration for international leadership in scientific research will be a guiding principle in our strategic planning. This initiative will serve as a foundational reference for the Institute of Modern Physics in its strategic planning and goal-setting, ensuring alignment with its developmental objectives while striving to secure a competitive edge in technological advancement. Our ambition is to engage in substantive research within these realms of high-precision physics, to pursue groundbreaking discoveries, and to stimulate progress in China's nuclear physics landscape, positioning Huizhou as a preeminent global hub for advanced nuclear physics research.展开更多
Objective Overactive bladder,a storage syndrome characterized by urinary urgency,frequency,and nocturia with or without urgency urinary incontinence,severely affects the quality of life of patients.The aim of this stu...Objective Overactive bladder,a storage syndrome characterized by urinary urgency,frequency,and nocturia with or without urgency urinary incontinence,severely affects the quality of life of patients.The aim of this study was to investigate the role and mechanism of the C/EBP homologous protein in the overactive bladder.Methods An overactive bladder mouse model was established via the intraperitoneal injection of cyclophosphamide in wild-type and Chop-deficient mice.An in vitro model was established using interleukin(IL)-6-induced mouse bladder epithelial cells.Hematoxylin–eosin(HE)staining was used to assess bladder tissue damage,and ELISA was used to measure inflammatory cytokine levels.Western blot analysis was used to examine p-PERK,ATF-6,p-eIF2α,BiP,ATF-4,Bax,Bcl-2,and cleaved caspase-3 protein expression levels.TUNEL staining and flow cytometry were conducted to measure the degree of apoptosis in bladder epithelial cells and macrophages.Results C/EBP homologous protein levels were decreased in overactive bladder tissues;nevertheless,macrophage infiltration was found to be increased.Knockout of Chop exacerbated bladder dysfunction,tissue injury,macrophage infiltration,and bladder epithelial apoptosis and alleviated endoplasmic reticulum stress.Conclusions Chop deficiency exacerbates inflammation,injury,and bladder epithelial apoptosis in overactive bladder model mice by inhibiting endoplasmic reticulum stress.展开更多
Severe tissue defects present formidable challenges to human health,persisting as major contributors to mortality rates.The complex pathological microenvironment,particularly the disrupted immune landscape within thes...Severe tissue defects present formidable challenges to human health,persisting as major contributors to mortality rates.The complex pathological microenvironment,particularly the disrupted immune landscape within these defects,poses substantial hurdles to existing tissue regeneration strategies.However,the emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine,providing encouraging prospects for tissue regeneration and restoration.This review aims to gather recent advances in immunomodulatory nanomedicine to foster tissue regeneration.We begin by elucidating the distinctive features of the local immune microenvironment within defective tissues and its crucial role in tissue regeneration.Subsequently,we explore the design and functional properties of immunomodulatory nanosystems.Finally,we address the challenges and prospects of clinical translation in nanomedicine development,aiming to propose a potent approach to enhance tissue regeneration through synergistic immune modulation and nanomedicine integration.展开更多
Hard carbons are promising anode materials for sodium-ion batteries(SIBs),but they face challenges in balancing rate capability,specific capacity,and initial Coulombic efficiency(ICE).Direct pyrolysis of the precursor...Hard carbons are promising anode materials for sodium-ion batteries(SIBs),but they face challenges in balancing rate capability,specific capacity,and initial Coulombic efficiency(ICE).Direct pyrolysis of the precursor often fails to create a suitable structure for sodium-ion storage.Molecular-level control of graphitization with open channels for Na^(+)ions is crucial for high-performance hard carbon,whereas closed pores play a key role in improving the low-voltage(<0.1 V)plateau capacity of hard carbon anodes for SIBs.However,creation of these closed pores presents significant challenges.This work proposes a zinc gluconate-assisted catalytic carbonization strategy to regulate graphitization and create numerous nanopores simultaneously.As the temperature increases,trace amounts of zinc remain as single atoms in the hard carbon,featuring a uniform coordination structure.This mitigates the risk of electrochemically irreversible sites and enhances sodium-ion transport rates.The resulting hard carbon shows an excellent reversible capacity of 348.5 mAh g^(-1) at 30 mA g^(-1) and a high ICE of 92.84%.Furthermore,a sodium storage mechanism involving“adsorption-intercalation-pore filling”is elucidated,providing insights into the pore structure and dynamic pore-filling process.展开更多
We propose a strategy to reduce unnecessary prostate biopsies in Chinese patients with total prostate-specific antigen(tPSA)>10 ng ml−1 and Prostate Imaging Reporting and Data System(PI-RADS)scores between 1 and 3....We propose a strategy to reduce unnecessary prostate biopsies in Chinese patients with total prostate-specific antigen(tPSA)>10 ng ml−1 and Prostate Imaging Reporting and Data System(PI-RADS)scores between 1 and 3.Clinical data derived from 517 patients of The First Affiliated Hospital of USTC(Hefei,China)from January 2020 to December 2023 who met the screening criteria for the study were retrospectively collected.Independent predictors were identified via univariate and multivariate logistic regression analysis.The diagnostic capacity of clinical variables was evaluated using the receiver operating characteristic(ROC)curves and area under the curve(AUC).A prostate biopsy strategy was developed via risk stratification.Of the 517 patients,17/348(4.9%)with PI-RADS 1–2 were diagnosed with clinically significant prostate cancer(csPCa),and 27/169(16.0%)patients with PI-RADS 3 were diagnosed with csPCa.The appropriate prostate-specific antigen density(PSAD)cut-off values were 0.45 ng ml−2 for PI-RADS 1–2 patients and 0.3 ng ml−2 for PI-RADS 3 patients.The appropriate prostate volume(PV)cut-off values were 40 ml for PI-RADS 1–2 patients and 50 ml for PI-RADS 3 patients.The prostate biopsy strategy based on PSAD and PV developed in this study can reduce unnecessary prostate biopsies in patients with tPSA>10 ng ml−1 and PI-RADS 1–3.In the study,66.5%(344/517)patients did not need to undergo prostate biopsy,at the expense of missing only 1.7%(6/344)patients with csPCa.展开更多
Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This st...Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This study aimed to elucidate the critical roles of Myb-like,SWIRM,and MPN domains 1(MYSM1)and its downstream effector,Receptor-interacting protein kinase 2(RIPK2),in OA pathogenesis and the underlying mechanisms.Our findings revealed reduced MYSM1 levels in the cartilage of OA patients and mouse models.Genetic or adenovirus-induced MYSM1 knockout exacerbated OA progression in mice,whereas MYSM1 overexpression mitigated it.Mechanistically,MYSM1 inhibited the NF-κB and MAPK signaling pathways.Conversely,downstream RIPK2 significantly increased OA-like phenotypes and activated the NF-κB and MAPK pathways.The Ripk2^(S176D) mutation accelerated OA pathogenesis,while Ripk2 silencing or Ripk2^(S176A)mutation deactivated NF-κB and MAPK pathways,counteracting the role of MYSM1.MYSM1 deubiquitinates and dephosphorylates RIPK2^(S176)by recruiting protein phosphatase 2 A(PP2A).These results suggest that targeting MYSM1 or downstream RIPK2 offers promising therapeutic potential for OA.展开更多
In the context of the Diagnosis Related Groups(DRGs)system,the orthopedic hospital implemented refined drug control to provide a pharmacological reference for promoting rational clinical drug use.A statistical analysi...In the context of the Diagnosis Related Groups(DRGs)system,the orthopedic hospital implemented refined drug control to provide a pharmacological reference for promoting rational clinical drug use.A statistical analysis was conducted on the hospital’s data from January to December 2021(prior to the implementation of control),focusing on the types of unreasonable prescriptions.A multi-dimensional analysis was also conducted to identify the underlying causes of inappropriate medication practices.Following this,refined drug control measures were introduced,and data from January to December 2022(post-control)were compared,examining factors such as the average drug cost,drug expenses for the IC29 diagnosis group,and the drug cost ratio.An interrupted time-series analysis was employed to evaluate the effects of these interventions.The results showed that after the implementation of refined drug control in the orthopedic department,significant reductions were observed in the average cost per patient,average drug cost per patient,drug cost ratio,cost consumption index,average length of hospital stay,and allocation ratio(P<0.05).In particular,the first month of control(January 2022)saw a marked decrease in average drug costs per patient by 1272.90 yuan(P<0.01),a reduction in the drug cost ratio by 0.98%,and a decline in drug costs for the IC29 diagnosis group by 616.79 yuan(P>0.05).Moreover,the rate of unreasonable inappropriate prescribing dropped dramatically from 40.48%in 2021 to 3.57%by December 2022.The refined control of drug use within the orthopedic hospital significantly improved the rationality of clinical prescribing practices,reduced the occurrence of adverse drug reactions,and enhanced patient adherence to prescribed treatments.These findings demonstrated considerable clinical value in promoting efficient and safe drug use.展开更多
Loads in large scale industrial enterprises are always various and complex, causing insecure power quality. Meanwhile, some sensitive loads or precise instruments demand for high power quality. When voltage flicker or...Loads in large scale industrial enterprises are always various and complex, causing insecure power quality. Meanwhile, some sensitive loads or precise instruments demand for high power quality. When voltage flicker or harmonics occurs, dynamic voltage restorer (DVR) can be used to stabilize the load-side voltage, which is a good manner of improving power quality. The paper explains the load demand in large scale industrial enterprises, analyzes several common voltage compensation scheme of DVR, and comes up with another voltage compensation scheme of DVR applied for double power supply with double load. A Simulink model according to the new scheme is also built in MATLAB/Simulink to test its performance. Simulation results show that the proposed scheme can guarantee the load-side voltage stability, which is an effective scheme for managing power quality.展开更多
Cancer has become a cause of concern in recent years. Cancer genomics is currently a key research direction in the fields of genetic biology and biomedicine. This paper analyzes 5 different types of cancer genes, such...Cancer has become a cause of concern in recent years. Cancer genomics is currently a key research direction in the fields of genetic biology and biomedicine. This paper analyzes 5 different types of cancer genes, such as breast, kidney, colon, lung and prostate through machine learning methods, with the goal of building a robust classification model to identify each type of cancer, which will allow us to identify each type of cancer early, thereby reducing mortality.展开更多
Traffic sign recognition is an important task in intelligent transportation systems, which can improve road safety and reduce accidents. Algorithms based on deep learning have achieved remarkable results in traffic si...Traffic sign recognition is an important task in intelligent transportation systems, which can improve road safety and reduce accidents. Algorithms based on deep learning have achieved remarkable results in traffic sign recognition in recent years. In this paper, we build traffic sign recognition algorithms based on ResNet and CNN models, respectively. We evaluate the proposed algorithm on public datasets and compare. We first use the dataset of traffic sign images from Kaggle. And then designed ResNet-based and CNN-based architectures that can effectively capture the complex features of traffic signs. Our experiments show that our ResNet-based model achieves a recognition accuracy of 99% on the test set, and our CNN-based model achieves a recognition accuracy of 98% on the test set. Our proposed approach has the potential to improve traffic safety and can be used in various intelligent transportation systems.展开更多
The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for g...The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for gas exploration and production. The gravity flow sedimentary characteristics of lithofacies associations, sedimentary texture, seismic facies and logging facies were described in detail on the basis of integrated analysis of cores, logging and seismic data. The sedimentary microfacies types composed of neritic sandbar, continental shelf mud, main channel, bifurcated or cross-cutting distributary channel, overspill, and natural levee are revealed under the constraint of high resolution sequence stratigraphic framework in the Ehl1. The gravity flow deposit system in the LST is divided into three evolution stages corresponding to periods of three parasequence sets. The gravity flow deposit was induced in the early LST, expanded rapidly in the middle LST and decreased slightly in the late LST. But its developing scale decreased sharply in the transgression systems tract（TST） and finally vanished in the highstand systems tract（HST）. This spatial evolution rule is constrained by the integrated function of sediments supply of the Vietnam Blue River in the LST, the development of local gradient change in sea floor（micro-topography, i.e., flexure slope break）, and the fall in relative sea level. On the basics of the deep study of the coupling relationship among the three main control factors, the sedimentary model is established as an optimal component of ＂source-channel-sink＂ for shallow marine turbidite submarine fan.展开更多
Visual fixation is an item in the visual function subscale of the Coma Recovery Scale-Revised （CRS-R）. Sometimes clinicians using the behavioral scales find it difficult to detect because of the motor impairment in ...Visual fixation is an item in the visual function subscale of the Coma Recovery Scale-Revised （CRS-R）. Sometimes clinicians using the behavioral scales find it difficult to detect because of the motor impairment in patients with disorders of consciousness （DOCs）. Brain- computer interface （BCI） can be used to improve clinical assessment because it directly detects the brain response to an external stimulus in the absence of behavioral expres- sion. In this study, we designed a BCI system to assist the visual fixation assessment of DOC patients. The results from 15 patients indicated that three showed visual fixation in both CRS-R and BCI assessments and one did not show such behavior in the CRS-R assessment but achieved significant online accuracy in the BCI assessment. The results revealed that electroencephalography-based BCI can detect the brain response for visual fixation. Therefore, the proposed BCI may provide a promising method for assisting behavioral assessment using the CRS-R.展开更多
Alzheimer’s disease(AD)is a degenerative neurological disease that primarily affects the elderly.Drug therapy is the main strategy for AD treatment,but current treatments suffer from poor efficacy and a number of sid...Alzheimer’s disease(AD)is a degenerative neurological disease that primarily affects the elderly.Drug therapy is the main strategy for AD treatment,but current treatments suffer from poor efficacy and a number of side effects.Non-drug therapy is attracting more attention and may be a better strategy for treatment of AD.Hypoxia is one of the important factors that contribute to the pathogenesis of AD.Multiple cellular processes synergistically promote hypoxia,including aging,hypertension,diabetes,hypoxia/obstructive sleep apnea,obesity,and traumatic brain injury.Increasing evidence has shown that hypoxia may affect multiple pathological aspects of AD,such as amyloid-beta metabolism,tau phosphorylation,autophagy,neuroinflammation,oxidative stress,endoplasmic reticulum stress,and mitochondrial and synaptic dysfunction.Treatments targeting hypoxia may delay or mitigate the progression of AD.Numerous studies have shown that oxygen therapy could improve the risk factors and clinical symptoms of AD.Increasing evidence also suggests that oxygen therapy may improve many pathological aspects of AD including amyloid-beta metabolism,tau phosphorylation,neuroinflammation,neuronal apoptosis,oxidative stress,neurotrophic factors,mitochondrial function,cerebral blood volume,and protein synthesis.In this review,we summarized the effects of oxygen therapy on AD pathogenesis and the mechanisms underlying these alterations.We expect that this review can benefit future clinical applications and therapy strategies on oxygen therapy for AD.展开更多
文摘BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility disturbance.The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal cells.Moreover,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal cells.Cajal cells are the pacemakers of gastrointestinal motility.AIM To investigate the effects of Bifidobacterium lactis(B.lactis)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit pathway.METHODS The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 days.The tail group was given B.lactis BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/day.Hematoxylin&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and PCR.The fecal flora and mucosal flora of rats in each group were detected by 16S rRNA.RESULTS Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic intervention.Simulated microgravity reduced the expressions of SCF and c-kit,and increased the expressions of ERK and P-ERK in the gastric antrum of rats.However,after probiotic intervention,the expressions of SCF and ckit were increased,while the expressions of ERK and P-ERK were decreased,with statistical significance(P<0.05).In addition,simulated microgravity can reduce the operational taxonomic unit(OTU)of the overall intestinal flora of rats,B.lactis BLa80 can increase the OTU of rats,simulated microgravity can reduce the overall richness and diversity of stool flora of rats,increase the abundance of firmicutes in stool flora of rats,and reduce the abundance of Bacteroides in stool flora of rats,most of which are mainly beneficial bacteria.Simulated microgravity can increase the overall richness and diversity of mucosal flora,increase the abundance of Bacteroides and Desulphurides in the rat mucosal flora,and decrease the abundance of firmicutes,most of which are proteobacteria.After probiotics intervention,the overall Bacteroidetes trend in simulated microgravity rats was increased.CONCLUSION B.lactis BLa80 can ameliorate intestinal mucosal injury,regulate intestinal flora,inhibit ERK expression,and activate the SCF/c-kit signaling pathway,which may have a facilitating effect on gastrointestinal motility in simulated microgravity rats.
基金supported by the National Natural Science Foundation of China (Grant No.12075326)the Guangdong Basic and Applied Basic Research Foundation (Grant No.2025A1515010669)+2 种基金the Natural Science Foundation of Guangzhou (Grant No.2024A04J6243)the Fundamental Research Funds for the Central Universities in Sun Yat-sen University (No.23xkjc017)the Innovation Training Program for bachelor students in Sun Yat-sen University。
文摘In response to the capabilities presented by the High-Intensity Heavy Ion Accelerator Facility(HIAF) and the Accelerator-Driven Subcritical System(Ci ADS), as well as the proposed Chinese Advanced Nuclear Physics Research Facility(CNUF), we are assembling a consortium of experts in relevant disciplines, both domestically and internationally,to delineate high-precision physics experiments that leverage the state-of-the-art research environment afforded by CNUF.Our focus encompasses six primary domains of inquiry: hadron physics—including endeavors such as the super eta factory and investigations into light hadron structures;muon physics;neutrino physics;neutron physics;the testing of fundamental symmetries;and the exploration of quantum effects within nuclear physics, along with the utilization of vortex accelerators.We aim to foster a well-rounded portfolio of large, medium, and small-scale projects, thus unlocking new scientific avenues and optimizing the potential of the Huizhou large scientific facility. The aspiration for international leadership in scientific research will be a guiding principle in our strategic planning. This initiative will serve as a foundational reference for the Institute of Modern Physics in its strategic planning and goal-setting, ensuring alignment with its developmental objectives while striving to secure a competitive edge in technological advancement. Our ambition is to engage in substantive research within these realms of high-precision physics, to pursue groundbreaking discoveries, and to stimulate progress in China's nuclear physics landscape, positioning Huizhou as a preeminent global hub for advanced nuclear physics research.
基金supported by the National Natural Science Foundation of China(No.81974400 and No.82173068).
文摘Objective Overactive bladder,a storage syndrome characterized by urinary urgency,frequency,and nocturia with or without urgency urinary incontinence,severely affects the quality of life of patients.The aim of this study was to investigate the role and mechanism of the C/EBP homologous protein in the overactive bladder.Methods An overactive bladder mouse model was established via the intraperitoneal injection of cyclophosphamide in wild-type and Chop-deficient mice.An in vitro model was established using interleukin(IL)-6-induced mouse bladder epithelial cells.Hematoxylin–eosin(HE)staining was used to assess bladder tissue damage,and ELISA was used to measure inflammatory cytokine levels.Western blot analysis was used to examine p-PERK,ATF-6,p-eIF2α,BiP,ATF-4,Bax,Bcl-2,and cleaved caspase-3 protein expression levels.TUNEL staining and flow cytometry were conducted to measure the degree of apoptosis in bladder epithelial cells and macrophages.Results C/EBP homologous protein levels were decreased in overactive bladder tissues;nevertheless,macrophage infiltration was found to be increased.Knockout of Chop exacerbated bladder dysfunction,tissue injury,macrophage infiltration,and bladder epithelial apoptosis and alleviated endoplasmic reticulum stress.Conclusions Chop deficiency exacerbates inflammation,injury,and bladder epithelial apoptosis in overactive bladder model mice by inhibiting endoplasmic reticulum stress.
基金supported by the National Science Foundation of China(82202714).
文摘Severe tissue defects present formidable challenges to human health,persisting as major contributors to mortality rates.The complex pathological microenvironment,particularly the disrupted immune landscape within these defects,poses substantial hurdles to existing tissue regeneration strategies.However,the emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine,providing encouraging prospects for tissue regeneration and restoration.This review aims to gather recent advances in immunomodulatory nanomedicine to foster tissue regeneration.We begin by elucidating the distinctive features of the local immune microenvironment within defective tissues and its crucial role in tissue regeneration.Subsequently,we explore the design and functional properties of immunomodulatory nanosystems.Finally,we address the challenges and prospects of clinical translation in nanomedicine development,aiming to propose a potent approach to enhance tissue regeneration through synergistic immune modulation and nanomedicine integration.
基金supported by the National Natural Science Foundation of China(22209103)Science and Technology Commission of Shanghai Municipality(22010500400)Australian Research Council(FT180100705)。
文摘Hard carbons are promising anode materials for sodium-ion batteries(SIBs),but they face challenges in balancing rate capability,specific capacity,and initial Coulombic efficiency(ICE).Direct pyrolysis of the precursor often fails to create a suitable structure for sodium-ion storage.Molecular-level control of graphitization with open channels for Na^(+)ions is crucial for high-performance hard carbon,whereas closed pores play a key role in improving the low-voltage(<0.1 V)plateau capacity of hard carbon anodes for SIBs.However,creation of these closed pores presents significant challenges.This work proposes a zinc gluconate-assisted catalytic carbonization strategy to regulate graphitization and create numerous nanopores simultaneously.As the temperature increases,trace amounts of zinc remain as single atoms in the hard carbon,featuring a uniform coordination structure.This mitigates the risk of electrochemically irreversible sites and enhances sodium-ion transport rates.The resulting hard carbon shows an excellent reversible capacity of 348.5 mAh g^(-1) at 30 mA g^(-1) and a high ICE of 92.84%.Furthermore,a sodium storage mechanism involving“adsorption-intercalation-pore filling”is elucidated,providing insights into the pore structure and dynamic pore-filling process.
基金supported by the National Natural Science Foundation of China(No.82072807)the Scientific Research Project of Universities of the Department of Education of Anhui Province(No.2022AH040182)the Anhui Province Key Clinical Specialties Construction Project(2023).
文摘We propose a strategy to reduce unnecessary prostate biopsies in Chinese patients with total prostate-specific antigen(tPSA)>10 ng ml−1 and Prostate Imaging Reporting and Data System(PI-RADS)scores between 1 and 3.Clinical data derived from 517 patients of The First Affiliated Hospital of USTC(Hefei,China)from January 2020 to December 2023 who met the screening criteria for the study were retrospectively collected.Independent predictors were identified via univariate and multivariate logistic regression analysis.The diagnostic capacity of clinical variables was evaluated using the receiver operating characteristic(ROC)curves and area under the curve(AUC).A prostate biopsy strategy was developed via risk stratification.Of the 517 patients,17/348(4.9%)with PI-RADS 1–2 were diagnosed with clinically significant prostate cancer(csPCa),and 27/169(16.0%)patients with PI-RADS 3 were diagnosed with csPCa.The appropriate prostate-specific antigen density(PSAD)cut-off values were 0.45 ng ml−2 for PI-RADS 1–2 patients and 0.3 ng ml−2 for PI-RADS 3 patients.The appropriate prostate volume(PV)cut-off values were 40 ml for PI-RADS 1–2 patients and 50 ml for PI-RADS 3 patients.The prostate biopsy strategy based on PSAD and PV developed in this study can reduce unnecessary prostate biopsies in patients with tPSA>10 ng ml−1 and PI-RADS 1–3.In the study,66.5%(344/517)patients did not need to undergo prostate biopsy,at the expense of missing only 1.7%(6/344)patients with csPCa.
基金National Natural Science Foundation of China(Nos.82330075 and 81401047)Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20241275)。
文摘Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This study aimed to elucidate the critical roles of Myb-like,SWIRM,and MPN domains 1(MYSM1)and its downstream effector,Receptor-interacting protein kinase 2(RIPK2),in OA pathogenesis and the underlying mechanisms.Our findings revealed reduced MYSM1 levels in the cartilage of OA patients and mouse models.Genetic or adenovirus-induced MYSM1 knockout exacerbated OA progression in mice,whereas MYSM1 overexpression mitigated it.Mechanistically,MYSM1 inhibited the NF-κB and MAPK signaling pathways.Conversely,downstream RIPK2 significantly increased OA-like phenotypes and activated the NF-κB and MAPK pathways.The Ripk2^(S176D) mutation accelerated OA pathogenesis,while Ripk2 silencing or Ripk2^(S176A)mutation deactivated NF-κB and MAPK pathways,counteracting the role of MYSM1.MYSM1 deubiquitinates and dephosphorylates RIPK2^(S176)by recruiting protein phosphatase 2 A(PP2A).These results suggest that targeting MYSM1 or downstream RIPK2 offers promising therapeutic potential for OA.
基金Jiangxi Provincial Hospital Pharmacy Special Research Fund Project(Grant No.2024-ZXYJ02).
文摘In the context of the Diagnosis Related Groups(DRGs)system,the orthopedic hospital implemented refined drug control to provide a pharmacological reference for promoting rational clinical drug use.A statistical analysis was conducted on the hospital’s data from January to December 2021(prior to the implementation of control),focusing on the types of unreasonable prescriptions.A multi-dimensional analysis was also conducted to identify the underlying causes of inappropriate medication practices.Following this,refined drug control measures were introduced,and data from January to December 2022(post-control)were compared,examining factors such as the average drug cost,drug expenses for the IC29 diagnosis group,and the drug cost ratio.An interrupted time-series analysis was employed to evaluate the effects of these interventions.The results showed that after the implementation of refined drug control in the orthopedic department,significant reductions were observed in the average cost per patient,average drug cost per patient,drug cost ratio,cost consumption index,average length of hospital stay,and allocation ratio(P<0.05).In particular,the first month of control(January 2022)saw a marked decrease in average drug costs per patient by 1272.90 yuan(P<0.01),a reduction in the drug cost ratio by 0.98%,and a decline in drug costs for the IC29 diagnosis group by 616.79 yuan(P>0.05).Moreover,the rate of unreasonable inappropriate prescribing dropped dramatically from 40.48%in 2021 to 3.57%by December 2022.The refined control of drug use within the orthopedic hospital significantly improved the rationality of clinical prescribing practices,reduced the occurrence of adverse drug reactions,and enhanced patient adherence to prescribed treatments.These findings demonstrated considerable clinical value in promoting efficient and safe drug use.
文摘Loads in large scale industrial enterprises are always various and complex, causing insecure power quality. Meanwhile, some sensitive loads or precise instruments demand for high power quality. When voltage flicker or harmonics occurs, dynamic voltage restorer (DVR) can be used to stabilize the load-side voltage, which is a good manner of improving power quality. The paper explains the load demand in large scale industrial enterprises, analyzes several common voltage compensation scheme of DVR, and comes up with another voltage compensation scheme of DVR applied for double power supply with double load. A Simulink model according to the new scheme is also built in MATLAB/Simulink to test its performance. Simulation results show that the proposed scheme can guarantee the load-side voltage stability, which is an effective scheme for managing power quality.
文摘Cancer has become a cause of concern in recent years. Cancer genomics is currently a key research direction in the fields of genetic biology and biomedicine. This paper analyzes 5 different types of cancer genes, such as breast, kidney, colon, lung and prostate through machine learning methods, with the goal of building a robust classification model to identify each type of cancer, which will allow us to identify each type of cancer early, thereby reducing mortality.
文摘Traffic sign recognition is an important task in intelligent transportation systems, which can improve road safety and reduce accidents. Algorithms based on deep learning have achieved remarkable results in traffic sign recognition in recent years. In this paper, we build traffic sign recognition algorithms based on ResNet and CNN models, respectively. We evaluate the proposed algorithm on public datasets and compare. We first use the dataset of traffic sign images from Kaggle. And then designed ResNet-based and CNN-based architectures that can effectively capture the complex features of traffic signs. Our experiments show that our ResNet-based model achieves a recognition accuracy of 99% on the test set, and our CNN-based model achieves a recognition accuracy of 98% on the test set. Our proposed approach has the potential to improve traffic safety and can be used in various intelligent transportation systems.
基金supported by the National Natural Science Foundation of China (NSFC) (Nos. 41272122, 41202074, 41172123 and 40702023)the National Twelfth Five-Year Major Projects of Oil and Gas (No. 2011ZX05025-002-02-02)+1 种基金the Key Laboratory of Tectonics and Petroleum Resources (CUG)of Ministry of Education open issue (No. TPR-2013-08)the China University of Geosciences (Wuhan) Teaching Labor atory open funded projects
文摘The gravity flow deposit were mainly developed in the lowstand systems tract（LST） of the first member of Upper Miocene Huangliu Formation（Ehl1） in Dongfang area, Yinggehai Basin, has become a valuable target for gas exploration and production. The gravity flow sedimentary characteristics of lithofacies associations, sedimentary texture, seismic facies and logging facies were described in detail on the basis of integrated analysis of cores, logging and seismic data. The sedimentary microfacies types composed of neritic sandbar, continental shelf mud, main channel, bifurcated or cross-cutting distributary channel, overspill, and natural levee are revealed under the constraint of high resolution sequence stratigraphic framework in the Ehl1. The gravity flow deposit system in the LST is divided into three evolution stages corresponding to periods of three parasequence sets. The gravity flow deposit was induced in the early LST, expanded rapidly in the middle LST and decreased slightly in the late LST. But its developing scale decreased sharply in the transgression systems tract（TST） and finally vanished in the highstand systems tract（HST）. This spatial evolution rule is constrained by the integrated function of sediments supply of the Vietnam Blue River in the LST, the development of local gradient change in sea floor（micro-topography, i.e., flexure slope break）, and the fall in relative sea level. On the basics of the deep study of the coupling relationship among the three main control factors, the sedimentary model is established as an optimal component of ＂source-channel-sink＂ for shallow marine turbidite submarine fan.
基金supported by the National Key Research and Development Program of China (2017YFB1002505)the National Natural Science Foundation of China (61633010, 91420302, and 61503143)+1 种基金the Natural Science Foundation of Guangdong Province, China (2014A030312005 and 2014A030310244)the Pearl River S&T Nova Program of Guangzhou Municipality, China (201710010038)
文摘Visual fixation is an item in the visual function subscale of the Coma Recovery Scale-Revised （CRS-R）. Sometimes clinicians using the behavioral scales find it difficult to detect because of the motor impairment in patients with disorders of consciousness （DOCs）. Brain- computer interface （BCI） can be used to improve clinical assessment because it directly detects the brain response to an external stimulus in the absence of behavioral expres- sion. In this study, we designed a BCI system to assist the visual fixation assessment of DOC patients. The results from 15 patients indicated that three showed visual fixation in both CRS-R and BCI assessments and one did not show such behavior in the CRS-R assessment but achieved significant online accuracy in the BCI assessment. The results revealed that electroencephalography-based BCI can detect the brain response for visual fixation. Therefore, the proposed BCI may provide a promising method for assisting behavioral assessment using the CRS-R.
基金supported by the Key Research and Development Support Project of Chengdu Science and Technology Bureau, No. 2019-YF05-00655-SN (to WDL)the Key Project of the Medical Science Department, University of Electronic Science and Technology of China, No. ZYGX2020ZB035 (to WDL)
文摘Alzheimer’s disease(AD)is a degenerative neurological disease that primarily affects the elderly.Drug therapy is the main strategy for AD treatment,but current treatments suffer from poor efficacy and a number of side effects.Non-drug therapy is attracting more attention and may be a better strategy for treatment of AD.Hypoxia is one of the important factors that contribute to the pathogenesis of AD.Multiple cellular processes synergistically promote hypoxia,including aging,hypertension,diabetes,hypoxia/obstructive sleep apnea,obesity,and traumatic brain injury.Increasing evidence has shown that hypoxia may affect multiple pathological aspects of AD,such as amyloid-beta metabolism,tau phosphorylation,autophagy,neuroinflammation,oxidative stress,endoplasmic reticulum stress,and mitochondrial and synaptic dysfunction.Treatments targeting hypoxia may delay or mitigate the progression of AD.Numerous studies have shown that oxygen therapy could improve the risk factors and clinical symptoms of AD.Increasing evidence also suggests that oxygen therapy may improve many pathological aspects of AD including amyloid-beta metabolism,tau phosphorylation,neuroinflammation,neuronal apoptosis,oxidative stress,neurotrophic factors,mitochondrial function,cerebral blood volume,and protein synthesis.In this review,we summarized the effects of oxygen therapy on AD pathogenesis and the mechanisms underlying these alterations.We expect that this review can benefit future clinical applications and therapy strategies on oxygen therapy for AD.
