The sensitivity of phytoplankton growth to chemical stressors influences the composition and function of phytoplankton communities.In this study,we compared the growth,photosynthetic,and metabolic responses of the cya...The sensitivity of phytoplankton growth to chemical stressors influences the composition and function of phytoplankton communities.In this study,we compared the growth,photosynthetic,and metabolic responses of the cyanobacterium Microcystis aeruginosa FACHB-469and the green alga Raphidocelis subcapitata FACHB-271 to herbicide diuron.The 5-day growth inhibition test showed the M.aeruginosa FACHB-469 is more sensitive to diuron.Meanwhile,the photosynthetic performance,quantum yield and efficiencies,and structural indicators of the photosynthetic apparatus were influenced by environmentally relevant levels of diuron differently between the two phytoplankton.These photosynthetic alterations were more significant in the M.aeruginosa FACHB-469 than in the R.subcapitata FACHB-271 under the three levels of diuron(5,10,and 20μg/L).Cellular metabolism of the two phytoplankton was considerably disturbed after diuron exposure.The metabolism of amino acids,such as L-arginine,L-homoserine,and N-Acetyl-L-alanine,was significantly altered in the R.subcapitata FACHB-271,while only L-valine level was significantly altered in the M.aeruginosa FACHB-469.The levels of allantoic acid,epicatechin,and taurine were significantly downregulated in the R.subcapitata FACHB-271,while pyrimidine metabolism andγ-linolenic acid metabolism were altered in the M.aeruginosa FACHB-469.Overall,distinct responses in photosynthetic physiology and cellular metabolism are suggestive of invisible differences under environmentally relevant level of diuron,contributing to the differences in sensitivity of growth between the two phytoplankton under diuron.展开更多
Heart failure(HF)has emerged as one of the foremost global health threats due to its intricate pathophysiological mechanisms and multifactorial etiology.Adeno-sine triphosphate(ATP)-induced cell death represents a nov...Heart failure(HF)has emerged as one of the foremost global health threats due to its intricate pathophysiological mechanisms and multifactorial etiology.Adeno-sine triphosphate(ATP)-induced cell death represents a novel form of regulated cell deaths,marked by cellular energy depletion and metabolic dysregulation stemming from excessive ATP accumulation,identifying its uniqueness compared to other cell death processes modalities such as programmed cell death and necrosis.Growing evidence suggests that ATP-induced cell death(AICD)is predominantly governed by various biological pathways,including energy meta-bolism,redox homeostasis and intracellular calcium equilibrium.Recent research has shown that AICD is crucial in HF induced by pathological conditions like myocardial infarction,ischemia-reperfusion injury,and chemotherapy.Thus,it is essential to investigate the function of AICD in the pathogenesis of HF,as this may provide a foundation for the development of targeted therapies and novel treatment strategies.This review synthesizes current advancements in under-standing the link between AICD and HF,while further elucidating its invol-vement in cardiac remodeling and HF progression.展开更多
A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino ...A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-linking.The structure of this new chitosan derivative was characterized by Fourier transform infrared（FT-IR）and proton nuclear magnetic resonance（1H NMR）spectroscopy,and its physical properties were determined by X-ray diffraction（XRD）,differential scanning calorimetry（DSC）,and thermogravimetric analysis（TGA）.The thermal and chemical stabilities of the new derivative were improved compared with those of native chitosan.Assay of Escherichia coli adhesion on a film based on this chitosan derivative showed good adsorption and biofilm formation.展开更多
基金supported by the Technology Innovation Guidance Program of Jiangxi Province“Science and Technology&Water Conservancy”Joint Plan(No.2022KSG01004)the National Natural Science Foundation of China(No.42107150)+1 种基金China Postdoctoral Science Foundation(No.2022M713317)the National Key R&D Program of China(No.2020YFA0907400)。
文摘The sensitivity of phytoplankton growth to chemical stressors influences the composition and function of phytoplankton communities.In this study,we compared the growth,photosynthetic,and metabolic responses of the cyanobacterium Microcystis aeruginosa FACHB-469and the green alga Raphidocelis subcapitata FACHB-271 to herbicide diuron.The 5-day growth inhibition test showed the M.aeruginosa FACHB-469 is more sensitive to diuron.Meanwhile,the photosynthetic performance,quantum yield and efficiencies,and structural indicators of the photosynthetic apparatus were influenced by environmentally relevant levels of diuron differently between the two phytoplankton.These photosynthetic alterations were more significant in the M.aeruginosa FACHB-469 than in the R.subcapitata FACHB-271 under the three levels of diuron(5,10,and 20μg/L).Cellular metabolism of the two phytoplankton was considerably disturbed after diuron exposure.The metabolism of amino acids,such as L-arginine,L-homoserine,and N-Acetyl-L-alanine,was significantly altered in the R.subcapitata FACHB-271,while only L-valine level was significantly altered in the M.aeruginosa FACHB-469.The levels of allantoic acid,epicatechin,and taurine were significantly downregulated in the R.subcapitata FACHB-271,while pyrimidine metabolism andγ-linolenic acid metabolism were altered in the M.aeruginosa FACHB-469.Overall,distinct responses in photosynthetic physiology and cellular metabolism are suggestive of invisible differences under environmentally relevant level of diuron,contributing to the differences in sensitivity of growth between the two phytoplankton under diuron.
基金Supported by Science and Technology Department of Yunnan Province-Kunming Medical University,Kunming Medical Joint Special Project-Surface Project,No.202401AY070001-164Yunnan Provincial Clinical Research Center Cardiovascular Diseases-New Technology Research for Development Project for Diagnosis and Treatment Cardiovascular Diseases,No.202102AA310002the Key Technology Research and Device Development Project for Innovative Diagnosis and Treatment of Structural Heart Disease in the Southwest Plateau Region,No.202302AA310045.
文摘Heart failure(HF)has emerged as one of the foremost global health threats due to its intricate pathophysiological mechanisms and multifactorial etiology.Adeno-sine triphosphate(ATP)-induced cell death represents a novel form of regulated cell deaths,marked by cellular energy depletion and metabolic dysregulation stemming from excessive ATP accumulation,identifying its uniqueness compared to other cell death processes modalities such as programmed cell death and necrosis.Growing evidence suggests that ATP-induced cell death(AICD)is predominantly governed by various biological pathways,including energy meta-bolism,redox homeostasis and intracellular calcium equilibrium.Recent research has shown that AICD is crucial in HF induced by pathological conditions like myocardial infarction,ischemia-reperfusion injury,and chemotherapy.Thus,it is essential to investigate the function of AICD in the pathogenesis of HF,as this may provide a foundation for the development of targeted therapies and novel treatment strategies.This review synthesizes current advancements in under-standing the link between AICD and HF,while further elucidating its invol-vement in cardiac remodeling and HF progression.
基金supported by the National Natural Science Foundation of China(Nos.20975082and20775059)theMinistry of Education of the People's Republic of China(No.NCET-08-0464)+2 种基金the State Forestry Administration of the People's Republic of China(No.200904004)the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of EducationNorthwest Agricultural & Forestry University
文摘A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-linking.The structure of this new chitosan derivative was characterized by Fourier transform infrared（FT-IR）and proton nuclear magnetic resonance（1H NMR）spectroscopy,and its physical properties were determined by X-ray diffraction（XRD）,differential scanning calorimetry（DSC）,and thermogravimetric analysis（TGA）.The thermal and chemical stabilities of the new derivative were improved compared with those of native chitosan.Assay of Escherichia coli adhesion on a film based on this chitosan derivative showed good adsorption and biofilm formation.
