Objective: To investigate whether the side population cells (SP cells) exist in human gallbladder carcinoma cell line and the differences of drug resistance gene ABCG2 expression in SP cells, non-SP cells and GBC-SD c...Objective: To investigate whether the side population cells (SP cells) exist in human gallbladder carcinoma cell line and the differences of drug resistance gene ABCG2 expression in SP cells, non-SP cells and GBC-SD cell lines. Methods: Fluorescence activated cell sorter (FACS) was used to sort the SP and non-SP cells from GBC-SD cell line of gallbladder carcinoma of human being. Then, the sorting cells were cultured and detected the expression of ABCG2 gene among the SP cells, non-SP cells and GBC-SD cell lines by using reverse transcription polymerase chain reaction (RT-PCR), immunofluo-rescence chemistry, western blot and flow cytometry techniques. Results: A very small fraction cells (0.64 ± 0.08%) were iso-lated through FACS analysis which had the potency of stem cells and highly expressed ABCG2 gene (89.56 ± 3.86%). On the contrary, there were nearly no expression in non-SP cells (1.32 ± 0.49%) and lower expression in GBC-SD cell line (12.37 ± 1.61%). Conclusion: The side population cells that had the potency of stem cells existed in human gallbladder carcinoma cell line and over-expressed the drug resistance gene ABCG2. They may be play an important role in drug resistance of tumor.展开更多
In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamats...In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamatsu company(Hamamatsu Photonics K.K.(HPK))and 150 micro-channel plate PMTs(MCP-PMTs,GDB-6201)from the NNVT company(North Night Vision Technology Co.,Ltd.(NNVT))were tested.Subsequently,an afterpulse model was built according to the afterpulse time distribution and the probability of occurrence for these two types of PMTs.The average ratio of the total afterpulse charge with a delay between 0.5μs and 20μs to the primary pulse charge is∼5.7%(13.2%)for the tested MCPPMTs(dynode-PMTs).The JUNO experiment will deploy 20,01220-inch PMTs;this study will benefit detector simulation,event reconstruction,and data analysis regarding the JUNO experiment.展开更多
Source properties and stress fields are critical to understand fundamental mechanisms for fluid-induced earthquakes.In this study,we identify the focal mechanism solutions(FMSs)of 360 earthquakes with local magnitude ...Source properties and stress fields are critical to understand fundamental mechanisms for fluid-induced earthquakes.In this study,we identify the focal mechanism solutions(FMSs)of 360 earthquakes with local magnitude M_(L)≥1.5 in the Changning shale gas field from January 2016 to May 2017 by fitting three-component waveforms.We then constrain the directions of the maximum horizontal stress(σ_(H_(max)))for four dense earthquake clusters using the stress tensor inversion method.The stress drops of 121 earthquakes with M_(L)≥1.5 are calculated using the spectral ratio method.We examine the spatiotemporal heterogeneity of stress field,and discuss the cause of non-double-couple(non-DC)components in seismicity clusters.Following the Mohr-Coulomb criterion,we estimate the fluid overpressure thresholds from FMS for different seismic clusters,providing insights into potential physical mechanisms for induced seismicity.The FMS results indicate that shallow reverse earthquakes,with steep dip angles,characterize most events.The source mechanisms of earthquakes with M_(L)≥1.5 are dominated by DC components(>70%),but several earthquakes with M_(L)>3.0 and the microseismic events nearby during injection period display significant non-DC components(>30%).Stress inversion results reveal that the σ_(H_(max)) direction ranges from 120°to 128°.Stress drops of earthquakes range between 0.10 and 64.49 MPa,with high values occurring on reverse faults situated at a greater distance from the shale layer,accompanied by a moderate rotation(≤25°)in the trend of σ_(H_(max)).The seismic clusters close to the shale layer exhibit low fluid overpressure thresholds,prone to being triggered by high pore-pressure fluid.The integrated results suggest that the diffusion of high pore pressures is likely to be the primary factor for observed earthquakes.The present results are expected to offer valuable insights into the origin of anomalous seismicity near the shale gas sites.展开更多
Cerebral ischemia/reperfusion(I/R)injury is an important pathophysiological condition of ischemic stroke that involves a variety of physiological and pathological cell death pathways,including autophagy,apoptosis,necr...Cerebral ischemia/reperfusion(I/R)injury is an important pathophysiological condition of ischemic stroke that involves a variety of physiological and pathological cell death pathways,including autophagy,apoptosis,necroptosis,and phagoptosis,among which autophagy is the most studied.We have reviewed studies published in the past 5 years regarding the association between autophagy and cerebral I/R injury.To the best of our knowledge,this is the first review article summarizing potential candidates targeting autophagic pathways in the treatment of I/R injury post ischemic stroke.The findings of this review may help to better understand the pathogenesis and mechanisms of I/R events and bridge the gap between basic and translational research that may lead to the development of novel therapeutic approaches for I/R injury.展开更多
In this paper, the problem of cubature Kalman fusion filtering(CKFF) is addressed for multi-sensor systems under amplify-and-forward(AaF) relays. For the purpose of facilitating data transmission, AaF relays are utili...In this paper, the problem of cubature Kalman fusion filtering(CKFF) is addressed for multi-sensor systems under amplify-and-forward(AaF) relays. For the purpose of facilitating data transmission, AaF relays are utilized to regulate signal communication between sensors and filters. Here, the randomly varying channel parameters are represented by a set of stochastic variables whose occurring probabilities are permitted to exhibit bounded uncertainty. Employing the spherical-radial cubature principle, a local filter under AaF relays is initially constructed. This construction ensures and minimizes an upper bound of the filtering error covariance by designing an appropriate filter gain. Subsequently, the local filters are fused through the application of the covariance intersection fusion rule. Furthermore, the uniform boundedness of the filtering error covariance's upper bound is investigated through establishing certain sufficient conditions. The effectiveness of the proposed CKFF scheme is ultimately validated via a simulation experiment concentrating on a three-phase induction machine.展开更多
Dear Editor,This letter deals with the distributed recursive set-membership filtering(DRSMF)issue for state-saturated systems under encryption-decryption mechanism.To guarantee the data security,the encryption-decrypt...Dear Editor,This letter deals with the distributed recursive set-membership filtering(DRSMF)issue for state-saturated systems under encryption-decryption mechanism.To guarantee the data security,the encryption-decryption mechanism is considered in the signal transmission process.Specifically,a novel DRSMF scheme is developed such that,for both state saturation and encryption-decryption mechanism,the filtering error(FE)is limited to the ellipsoid domain.Then,the filtering error constraint matrix(FECM)is computed and a desirable filter gain is derived by minimizing the FECM.Besides,the bound-edness evaluation of the FECM is provided.展开更多
This study explores the adsorption and reac-tion of methanol on the CeO_(2)(111)and Ni/CeO_(2)(111)surfaces,highlighting the es-sential role of metal-support interaction in methanol decomposition by a synergistic ap-p...This study explores the adsorption and reac-tion of methanol on the CeO_(2)(111)and Ni/CeO_(2)(111)surfaces,highlighting the es-sential role of metal-support interaction in methanol decomposition by a synergistic ap-proach encompassing synchrotron radiation photoemission spectroscopy,X-ray photo-electron spectroscopy,infrared reflection and absorption spectroscopy,and temperature-programmed desorption.Our findings reveal that Ni deposited on the CeO_(2)(111)surface,followed by annealing to 700 K,leads to the formation of Ce-O-Ni mixed oxide as the dominant phase.The Ni^(2+)species facilitate the methoxy decomposition into CO and H_(2)within 300-430 K,with a small amount of formalde-hyde also forming at the edge sites of ceria.Additionally,some methoxy adsorbed on the bare CeO_(2)surface migrates to the Ce-O-Ni mixed oxide,where they decompose into CO and H_(2)at 500-600 K,accompanied by a portion of the methoxy interacting with ceria to generate formaldehyde.Upon exposure to methanol at 500 K,the Ni^(2+)species are reduced to metallic Ni^(0),alongside the formation of coke and Ni_(3)C,ultimately resulting in catalyst deactivation.However,reintroducing O_(2)reactivates these sites by oxidizing metallic Ni^(0)and Ni_(3)C species.This study highlights the pivotal role of metal-support interaction in promoting oxygen trans-fer from ceria to Ni,thereby enhancing methoxy decomposition and significantly improving the performance of Ni-based catalysts for methanol decomposition into CO and H_(2).展开更多
BACKGROUND Cholangiocarcinoma(CCA)is a highly aggressive malignancy with limited therapeutic options.Dysregulation of the Hippo-yes-associated protein(YAP)signaling pathway plays a key role in tumor progression,but th...BACKGROUND Cholangiocarcinoma(CCA)is a highly aggressive malignancy with limited therapeutic options.Dysregulation of the Hippo-yes-associated protein(YAP)signaling pathway plays a key role in tumor progression,but the effects of distinct bile acids on this pathway remain unclear.AIM To investigate the roles of glycochenodeoxycholic acid(GDCA)and deoxycholic acid(DCA)in CCA progression through Hippo-YAP signaling and to evaluate the effects of YAP-targeted interventions.METHODS The in vitro experiments were performed using HuCCT1 CCA cells treated with GDCA,DCA,and combinations with a YAP inhibitor(verteporfin)or agonist(GA-017).Key molecular changes in the Hippo-YAP pathway were assessed by western blot,immunofluorescence,and reverse transcription quantitative realtime polymerase chain reaction.Functional assays,including Cell Counting Kit-8,5-ethynyl-2’-deoxyuridine,Transwell,and terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate-nick end labelling,were conducted to evaluate cell proliferation,migration,invasion,and apoptosis.In vivo,nude mice bearing subcutaneous HuCCT1 tumors were treated with GDCA,DCA,or combined YAP modulators.Tumor growth was monitored,and molecular analyses of tumor tissues were performed using western blot.RESULTS The GDCA significantly activated YAP by reducing mammalian STE20-like protein kinase 1 and large tumor suppressor 1 phosphorylation,promoting YAP nuclear translocation,and enhancing tumor cell proliferation,migration,and invasion.In contrast,DCA inhibited YAP activation,suppressed tumor cell functions,and increased apoptosis.GDCA combined with YAP inhibitors attenuated its tumor-promoting effects,while DCA combined with YAP agonists reversed its inhibitory effects.In vivo,GDCA accelerated tumor growth,while DCA reduced tumor size and weight,with molecular changes consistent with in vitro findings.CONCLUSION The GDCA and DCA exert opposing effects on CCA progression through Hippo-YAP signaling.GDCA promotes tumor growth via YAP activation,while DCA inhibits these processes.YAP-targeted interventions demonstrate therapeutic potential,providing insights into new treatment strategies for CCA.展开更多
Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbull...Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbullying over a one-year period,drawing on the Stage-Environment Fit Theory and the Interpersonal Relationship Theory.Methods A three-wave longitudinal study was conducted over one year,involving 896 middle school students from five schools in Sichuan,Jiangsu,and Guangdong,China,selected to ensure regional diversity.Participants were recruited using stratified random sampling,and data were collected at four-month intervals.Leisure-time physical activity,peer relationships,and cyberbullying behaviors were assessed using validated scales.Data were analyzed using latent growth modeling(LGM)and structural equation modeling(SEM)to examine longitudinal effects and mediation relationships.Results(1)Leisure-time physical activity,peer relationships,and cyberbullying behaviors remained relatively stable over time.(2)Both leisure-time physical activity and peer relationships significantly reduced future cyberbullying incidents,with physical activity also enhancing subsequent peer relationships.(3)Peer relationships partially mediated the effect of leisure-time physical activity on cyberbullying,indicating that improved peer interactions contributed to a reduction in cyberbullying behaviors.Conclusion This study found that leisure-time sports activities and peer relationships are important factors affecting cyberbullying,and peer relationships play a partial mediating role in it.The results provide empirical support for understanding the formation mechanism and influencing factors of cyberbullying.展开更多
Gas storage in abandoned mines is one way to reuse waste space resources.The surrounding rock of gas storage reservoirs in underground roadways undergoes damage and deformation under the cyclic loading of gas charging...Gas storage in abandoned mines is one way to reuse waste space resources.The surrounding rock of gas storage reservoirs in underground roadways undergoes damage and deformation under the cyclic loading of gas charging and discharging,which can pose a risk to the safety of the reservoirs.This study establishes a true triaxial numerical model of rock mass with the discrete element method(DEM)and explores the crack evolution of surrounding rock of underground gas storage during cyclic loading and unloading.Also,a damage evolution model in numerical analysis considering residual deformation is developed to explain the experimental results.As was revealed,cyclic loading and unloading resulted in fatigue damage in the specimen and caused strength deterioration of the specimen.During the loading process,the uniformly distributed force chains of the rock mass redistributed,evolving gradually to mostly transverse force chains.This contributed to the appearance of blank areas in the force chains when through cracks appear.The ratio of tensile cracks to shear cracks gradually decreases and finally stabilizes at 7:1.The damage evolution model considering residual strain can be mutually verified with the numerical simulation results.Based on the DEM model,it was found that there was a certain threshold of confining pressure.When the confining pressure exceeded 30 MPa,the deformation to ductility of sandstone samples began to accelerate,with a greater residual strength.This study provides a theoretical basis for analyzing the long-term mechanical behavior of surrounding rock of gas storage in abandoned mines.展开更多
BACKGROUND Autoimmune encephalitis(AE)is a rare and recently described neuroinflammatory disease associated with specific autoantibodies.Anti-leucine-rich glioma inactivated 1(anti-LGI1)encephalitis is a rare but trea...BACKGROUND Autoimmune encephalitis(AE)is a rare and recently described neuroinflammatory disease associated with specific autoantibodies.Anti-leucine-rich glioma inactivated 1(anti-LGI1)encephalitis is a rare but treatable type of AE discovered in recent years.Alzheimer’s disease(AD)is a degenerative brain disease and the most common cause of dementia.AD may undergo a series of pathological physiological changes in brain tissue 20 years before the onset of typical symptoms.The stage of mild cognitive impairment(MCI)that occurs during this process,known as MCI due to AD,is the earliest stage with clinical symptoms.MCI is typically categorized into two subtypes:Amnestic MCI(aMCI)and non-aMCI.CASE SUMMARY This report describes a patient with rapid cognitive impairment,diagnosed with anti-LGI1 antibody-mediated AE and aMCI,and treated at Peking University Shenzhen Hospital in March 2023.The patient was hospitalized with acute memory decline for more than 3 months.Both the cerebrospinal fluid and serum were positive for anti-LGI1 antibodies,biomarkers of AD coexisting in the patient’s cerebrospinal fluid.Following combination treatment with immunoglobulin therapy and glucocorticoid,plus inhibition of acetylcholinesterase,the patient’s cognitive function significantly improved.Throughout the 3-month follow-up period,a sustained improvement in cognitive function was observed.The results of serum anti-LGI1 antibody were negative.CONCLUSION This case has raised awareness of the possible interaction between AE and early AD(including MCI due to AD),and alerted clinicians to the possibility of concurrent rare and common diseases in patients presenting with cognitive impairment.展开更多
Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breedin...Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.展开更多
Recycling-oriented alloy design is a crucial part of material sustainability,as it reduces the need for raw material extraction and minimises environmental impact.This requires that scraps be reused or repurposed effe...Recycling-oriented alloy design is a crucial part of material sustainability,as it reduces the need for raw material extraction and minimises environmental impact.This requires that scraps be reused or repurposed effectively,even when the scraps are co-mingled and have higher costs for further sorting and separation.In this work,we explore an alloy design concept by creating a compositionally flexible domain that can recycle multiple alloy grades and yet maintain relatively consistent properties across chemical variations.This is demonstrated through the Fe-Cr-Ni-Mn system to identify compositionally flexible austenitic stainless steels(CF-ASS)and accommodate the recycling of mixed austenitic stainless steel scraps.Alloys within the nominal composition spaces exhibit relatively consistent mechanical properties and corrosion resistance despite significant variations in different alloy compositions.We illustrate how we can utilise the compositionally flexible austenitic stainless steels to recycle mixed 200 and 300-series stainless steel and ferronickel scraps,demonstrating its practical viability.While this demonstration focuses on the stainless steel system,the underlying principles can be extended to other systems related to mixed scrap recycling.展开更多
Polyaniline(PANi)hydrogels have a wide range of applications in artificial skin,flexible robotics,and movement monitoring.Nevertheless,limited by the modulus mismatch between rigid PANi and the soft hydrogel matrix,th...Polyaniline(PANi)hydrogels have a wide range of applications in artificial skin,flexible robotics,and movement monitoring.Nevertheless,limited by the modulus mismatch between rigid PANi and the soft hydrogel matrix,the high strength and toughness of the PANi hydrogel are mutually exclusive.Although the introduction of sacrificial bonds into the hydrogel network can alleviate this contradiction to a certain extent,it always causes pronounced energy hysteresis during hydrogel deformation.Inspired by the energy storage and release of macroscopic springs,in this work,we propose a molecular entanglement approach for the fabrication of PANi hydrogels featuring high toughness and low hysteresis,where flexible poly(ethylene glycol)(PEG)is entangled with chemically cross-linked poly(acrylic acid)(PAA)as a hydrogel matrix,and rigid PANi as a conductive filler.The resultant PAA/PEG/PANi hydrogel exhibited high mechanical properties(fracture strength of 0.75 MPa and toughness of 4.81 MJ·m^(-3))and a low energy dissipation ratio(28.2%when stretching to 300%).Moreover,the PAA/PEG/PANi hydrogel possesses a good electrical response to external forces and can be employed as a strain sensor to monitor human joint movements by producing specific electrical signals.This work provides a straightforward strategy for preparing tough conductive PANi hydrogels with low hysteresis,showing potential for the development of healthcare devices.展开更多
The exploration of topological phases remains a cutting-edge research frontier,driven by their promising potential for next-generation electronic and quantum technologies.In this work,we employ first-principles calcul...The exploration of topological phases remains a cutting-edge research frontier,driven by their promising potential for next-generation electronic and quantum technologies.In this work,we employ first-principles calculations and tightbinding modeling to systematically investigate the topological properties of freestanding two-dimensional(2D)honeycomb Bi,HgTe,and Al_(2)O_(3)(0001)-supported HgTe.Remarkably,all three systems exhibit coexistence of intrinsic first-and higher-order topological insulator states,induced by spin-orbit coupling(SOC).These states manifest as topologically protected gapless edge states in one-dimensional(1D)nanoribbons and symmetry-related corner states in zero-dimensional(0D)nanoflakes.Furthermore,fractional electron charges may accumulate at the corners of armchair-edged nanoflakes.Among these materials,HgTe/Al_(2)O_(3)(0001)is particularly promising due to its experimentally feasible atomic configuration and low-energy corner states.Our findings highlight the importance of exploring higher-order topological phases in quantum spin Hall insulators and pave the way for new possibilities in device applications.展开更多
Total hip arthroplasty(THA)has limitations in grinding angles,prosthesis placements,and thickness variations.THA robotics offer promise but encounter challenges like manual control of the robotic arm for precise posit...Total hip arthroplasty(THA)has limitations in grinding angles,prosthesis placements,and thickness variations.THA robotics offer promise but encounter challenges like manual control of the robotic arm for precise positioning and potential over-grinding when controlled manually.This paper presents a THA surgical robot system with automatic positioning and automatic grinding and filing functions.It achieves precise positioning during the surgery by using the singular value decomposition of initial value screening and sliding mode control(SMC),and ensures uniformity,stability and controlled filing thickness through the designed end grinding and filing actuator system.It has been verified experimentally that the average position errors in the x,y,and z directions are 0.692 mm,0.512 mm,and 0.66 mm respectively,and the Euclidean distance error is 1.322 mm.The average angle error is less than 1.136°.The end effector can perform automatic grinding according to the predetermined planning value within the safe force threshold of 30 N.This THA surgical robot system can meet the requirements of the hip replacement surgery for the accuracy,driving ability and robustness of the system.展开更多
基金Supported in part by the National Natural Science Foundation of China (No. 30471963).
文摘Objective: To investigate whether the side population cells (SP cells) exist in human gallbladder carcinoma cell line and the differences of drug resistance gene ABCG2 expression in SP cells, non-SP cells and GBC-SD cell lines. Methods: Fluorescence activated cell sorter (FACS) was used to sort the SP and non-SP cells from GBC-SD cell line of gallbladder carcinoma of human being. Then, the sorting cells were cultured and detected the expression of ABCG2 gene among the SP cells, non-SP cells and GBC-SD cell lines by using reverse transcription polymerase chain reaction (RT-PCR), immunofluo-rescence chemistry, western blot and flow cytometry techniques. Results: A very small fraction cells (0.64 ± 0.08%) were iso-lated through FACS analysis which had the potency of stem cells and highly expressed ABCG2 gene (89.56 ± 3.86%). On the contrary, there were nearly no expression in non-SP cells (1.32 ± 0.49%) and lower expression in GBC-SD cell line (12.37 ± 1.61%). Conclusion: The side population cells that had the potency of stem cells existed in human gallbladder carcinoma cell line and over-expressed the drug resistance gene ABCG2. They may be play an important role in drug resistance of tumor.
基金supported by Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA10011100),Joint Institute of Nuclear Research (JINR),Russia and Lomonosov Moscow State University in Russia,joint Russian Science Foundation (RSF),DFG (Deutsche Forschungsgemeinschaft)National Natural Science Foundation of China (Nos. 12090062 and 12075087)
文摘In this study,we present the large photomultiplier tube(PMT)afterpulse measurement results obtained from the Jiangmen underground neutrino observatory(JUNO)experiment.A total of 11 dynode-PMTs(R12860)from the Hamamatsu company(Hamamatsu Photonics K.K.(HPK))and 150 micro-channel plate PMTs(MCP-PMTs,GDB-6201)from the NNVT company(North Night Vision Technology Co.,Ltd.(NNVT))were tested.Subsequently,an afterpulse model was built according to the afterpulse time distribution and the probability of occurrence for these two types of PMTs.The average ratio of the total afterpulse charge with a delay between 0.5μs and 20μs to the primary pulse charge is∼5.7%(13.2%)for the tested MCPPMTs(dynode-PMTs).The JUNO experiment will deploy 20,01220-inch PMTs;this study will benefit detector simulation,event reconstruction,and data analysis regarding the JUNO experiment.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.U20A20266 and 12302503)Scientific and technological research projects in Sichuan province(Grant No.2024NSFSC0973).
文摘Source properties and stress fields are critical to understand fundamental mechanisms for fluid-induced earthquakes.In this study,we identify the focal mechanism solutions(FMSs)of 360 earthquakes with local magnitude M_(L)≥1.5 in the Changning shale gas field from January 2016 to May 2017 by fitting three-component waveforms.We then constrain the directions of the maximum horizontal stress(σ_(H_(max)))for four dense earthquake clusters using the stress tensor inversion method.The stress drops of 121 earthquakes with M_(L)≥1.5 are calculated using the spectral ratio method.We examine the spatiotemporal heterogeneity of stress field,and discuss the cause of non-double-couple(non-DC)components in seismicity clusters.Following the Mohr-Coulomb criterion,we estimate the fluid overpressure thresholds from FMS for different seismic clusters,providing insights into potential physical mechanisms for induced seismicity.The FMS results indicate that shallow reverse earthquakes,with steep dip angles,characterize most events.The source mechanisms of earthquakes with M_(L)≥1.5 are dominated by DC components(>70%),but several earthquakes with M_(L)>3.0 and the microseismic events nearby during injection period display significant non-DC components(>30%).Stress inversion results reveal that the σ_(H_(max)) direction ranges from 120°to 128°.Stress drops of earthquakes range between 0.10 and 64.49 MPa,with high values occurring on reverse faults situated at a greater distance from the shale layer,accompanied by a moderate rotation(≤25°)in the trend of σ_(H_(max)).The seismic clusters close to the shale layer exhibit low fluid overpressure thresholds,prone to being triggered by high pore-pressure fluid.The integrated results suggest that the diffusion of high pore pressures is likely to be the primary factor for observed earthquakes.The present results are expected to offer valuable insights into the origin of anomalous seismicity near the shale gas sites.
基金Shanghai Rehabilitation Medical Association,Grant/Award Number:2023JGKT24China Rehabilitation Medical Association,Grant/Award Number:KFKT-2023Shanghai“14th Five-Year Plan”Traditional Chinese Medicine Specialty and Traditional Chinese Medicine Emergency Capacity Improvement Project,Grant/Award Number:ZYTSZK2-7。
文摘Cerebral ischemia/reperfusion(I/R)injury is an important pathophysiological condition of ischemic stroke that involves a variety of physiological and pathological cell death pathways,including autophagy,apoptosis,necroptosis,and phagoptosis,among which autophagy is the most studied.We have reviewed studies published in the past 5 years regarding the association between autophagy and cerebral I/R injury.To the best of our knowledge,this is the first review article summarizing potential candidates targeting autophagic pathways in the treatment of I/R injury post ischemic stroke.The findings of this review may help to better understand the pathogenesis and mechanisms of I/R events and bridge the gap between basic and translational research that may lead to the development of novel therapeutic approaches for I/R injury.
基金supported in part by the National Natural Science Foundation of China(12171124,61933007)the Natural Science Foundation of Heilongjiang Province of China(ZD2022F003)+2 种基金the National High-End Foreign Experts Recruitment Plan of China(G2023012004L)the Royal Society of UKthe Alexander von Humboldt Foundation of Germany
文摘In this paper, the problem of cubature Kalman fusion filtering(CKFF) is addressed for multi-sensor systems under amplify-and-forward(AaF) relays. For the purpose of facilitating data transmission, AaF relays are utilized to regulate signal communication between sensors and filters. Here, the randomly varying channel parameters are represented by a set of stochastic variables whose occurring probabilities are permitted to exhibit bounded uncertainty. Employing the spherical-radial cubature principle, a local filter under AaF relays is initially constructed. This construction ensures and minimizes an upper bound of the filtering error covariance by designing an appropriate filter gain. Subsequently, the local filters are fused through the application of the covariance intersection fusion rule. Furthermore, the uniform boundedness of the filtering error covariance's upper bound is investigated through establishing certain sufficient conditions. The effectiveness of the proposed CKFF scheme is ultimately validated via a simulation experiment concentrating on a three-phase induction machine.
基金supported by the National Natural Science Foundation of China(12471416,12171124,12301567)the Heilongjiang Provincial Natural Science Foundation of China(PL2024F015)+2 种基金the Postdoctoral Science Foundation of Heilongjiang Province of China(LBH-Z22199)the Fundamental Research Foun-dation for Universities of Heilongjiang Province of China(2022-KYYWF-0141)the Alexander von Humboldt Foundation of Germany.
文摘Dear Editor,This letter deals with the distributed recursive set-membership filtering(DRSMF)issue for state-saturated systems under encryption-decryption mechanism.To guarantee the data security,the encryption-decryption mechanism is considered in the signal transmission process.Specifically,a novel DRSMF scheme is developed such that,for both state saturation and encryption-decryption mechanism,the filtering error(FE)is limited to the ellipsoid domain.Then,the filtering error constraint matrix(FECM)is computed and a desirable filter gain is derived by minimizing the FECM.Besides,the bound-edness evaluation of the FECM is provided.
基金financially supported by the National Key R&D Program of China(2023YFA1509103)the National Natural Science Foundation of China(Nos.22272157,21872131,22106085,and U1932214)。
文摘This study explores the adsorption and reac-tion of methanol on the CeO_(2)(111)and Ni/CeO_(2)(111)surfaces,highlighting the es-sential role of metal-support interaction in methanol decomposition by a synergistic ap-proach encompassing synchrotron radiation photoemission spectroscopy,X-ray photo-electron spectroscopy,infrared reflection and absorption spectroscopy,and temperature-programmed desorption.Our findings reveal that Ni deposited on the CeO_(2)(111)surface,followed by annealing to 700 K,leads to the formation of Ce-O-Ni mixed oxide as the dominant phase.The Ni^(2+)species facilitate the methoxy decomposition into CO and H_(2)within 300-430 K,with a small amount of formalde-hyde also forming at the edge sites of ceria.Additionally,some methoxy adsorbed on the bare CeO_(2)surface migrates to the Ce-O-Ni mixed oxide,where they decompose into CO and H_(2)at 500-600 K,accompanied by a portion of the methoxy interacting with ceria to generate formaldehyde.Upon exposure to methanol at 500 K,the Ni^(2+)species are reduced to metallic Ni^(0),alongside the formation of coke and Ni_(3)C,ultimately resulting in catalyst deactivation.However,reintroducing O_(2)reactivates these sites by oxidizing metallic Ni^(0)and Ni_(3)C species.This study highlights the pivotal role of metal-support interaction in promoting oxygen trans-fer from ceria to Ni,thereby enhancing methoxy decomposition and significantly improving the performance of Ni-based catalysts for methanol decomposition into CO and H_(2).
文摘BACKGROUND Cholangiocarcinoma(CCA)is a highly aggressive malignancy with limited therapeutic options.Dysregulation of the Hippo-yes-associated protein(YAP)signaling pathway plays a key role in tumor progression,but the effects of distinct bile acids on this pathway remain unclear.AIM To investigate the roles of glycochenodeoxycholic acid(GDCA)and deoxycholic acid(DCA)in CCA progression through Hippo-YAP signaling and to evaluate the effects of YAP-targeted interventions.METHODS The in vitro experiments were performed using HuCCT1 CCA cells treated with GDCA,DCA,and combinations with a YAP inhibitor(verteporfin)or agonist(GA-017).Key molecular changes in the Hippo-YAP pathway were assessed by western blot,immunofluorescence,and reverse transcription quantitative realtime polymerase chain reaction.Functional assays,including Cell Counting Kit-8,5-ethynyl-2’-deoxyuridine,Transwell,and terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate-nick end labelling,were conducted to evaluate cell proliferation,migration,invasion,and apoptosis.In vivo,nude mice bearing subcutaneous HuCCT1 tumors were treated with GDCA,DCA,or combined YAP modulators.Tumor growth was monitored,and molecular analyses of tumor tissues were performed using western blot.RESULTS The GDCA significantly activated YAP by reducing mammalian STE20-like protein kinase 1 and large tumor suppressor 1 phosphorylation,promoting YAP nuclear translocation,and enhancing tumor cell proliferation,migration,and invasion.In contrast,DCA inhibited YAP activation,suppressed tumor cell functions,and increased apoptosis.GDCA combined with YAP inhibitors attenuated its tumor-promoting effects,while DCA combined with YAP agonists reversed its inhibitory effects.In vivo,GDCA accelerated tumor growth,while DCA reduced tumor size and weight,with molecular changes consistent with in vitro findings.CONCLUSION The GDCA and DCA exert opposing effects on CCA progression through Hippo-YAP signaling.GDCA promotes tumor growth via YAP activation,while DCA inhibits these processes.YAP-targeted interventions demonstrate therapeutic potential,providing insights into new treatment strategies for CCA.
基金funded by the following projects:National Social Science Fund Project“The Empowerment Mechanism of Physical Exercise on Emotional Regulation in Adolescents”(23BTY116)Leshan Normal University 2024 Research Cultivation Project:“Research on the Trajectory Effect of Family Cumulative Risk and Home-based Activity of Adolescents”(KYPY2025-0014)+1 种基金Key Humanities and Social Sciences Cultivation Project of Leshan Normal University:“Research on the Sequence Difference of Knowledge and Behavior of Physical Activity among Adolescents and the Compensation Mechanism”and Sichuan Province College Students’Sports Association Annual Project“The Trajectory Effect of Family Cumulative Risk and Adolescents’Home Physical Activity”(23CDTXQ004).
文摘Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbullying over a one-year period,drawing on the Stage-Environment Fit Theory and the Interpersonal Relationship Theory.Methods A three-wave longitudinal study was conducted over one year,involving 896 middle school students from five schools in Sichuan,Jiangsu,and Guangdong,China,selected to ensure regional diversity.Participants were recruited using stratified random sampling,and data were collected at four-month intervals.Leisure-time physical activity,peer relationships,and cyberbullying behaviors were assessed using validated scales.Data were analyzed using latent growth modeling(LGM)and structural equation modeling(SEM)to examine longitudinal effects and mediation relationships.Results(1)Leisure-time physical activity,peer relationships,and cyberbullying behaviors remained relatively stable over time.(2)Both leisure-time physical activity and peer relationships significantly reduced future cyberbullying incidents,with physical activity also enhancing subsequent peer relationships.(3)Peer relationships partially mediated the effect of leisure-time physical activity on cyberbullying,indicating that improved peer interactions contributed to a reduction in cyberbullying behaviors.Conclusion This study found that leisure-time sports activities and peer relationships are important factors affecting cyberbullying,and peer relationships play a partial mediating role in it.The results provide empirical support for understanding the formation mechanism and influencing factors of cyberbullying.
基金National Natural Science Foundation of China,Grant/Award Numbers:U22A20598,52104107National Key Research and Development Program of China,Grant/Award Numbers:2023YFC2907300,2019YFE0118500,2019YFC1904304Natural Science Foundation of Jiangsu Province,Grant/Award Number:BK20200634。
文摘Gas storage in abandoned mines is one way to reuse waste space resources.The surrounding rock of gas storage reservoirs in underground roadways undergoes damage and deformation under the cyclic loading of gas charging and discharging,which can pose a risk to the safety of the reservoirs.This study establishes a true triaxial numerical model of rock mass with the discrete element method(DEM)and explores the crack evolution of surrounding rock of underground gas storage during cyclic loading and unloading.Also,a damage evolution model in numerical analysis considering residual deformation is developed to explain the experimental results.As was revealed,cyclic loading and unloading resulted in fatigue damage in the specimen and caused strength deterioration of the specimen.During the loading process,the uniformly distributed force chains of the rock mass redistributed,evolving gradually to mostly transverse force chains.This contributed to the appearance of blank areas in the force chains when through cracks appear.The ratio of tensile cracks to shear cracks gradually decreases and finally stabilizes at 7:1.The damage evolution model considering residual strain can be mutually verified with the numerical simulation results.Based on the DEM model,it was found that there was a certain threshold of confining pressure.When the confining pressure exceeded 30 MPa,the deformation to ductility of sandstone samples began to accelerate,with a greater residual strength.This study provides a theoretical basis for analyzing the long-term mechanical behavior of surrounding rock of gas storage in abandoned mines.
基金Supported by the Shenzhen Science and Technology Program,No.JCYJ20220818102810022Shenzhen University Teaching Reform fund,No.JG2023166+1 种基金National Natural Science Foundation of China,No.12231018Science and Technology Innovation 2030-Major Project,No.2021ZD0201805.
文摘BACKGROUND Autoimmune encephalitis(AE)is a rare and recently described neuroinflammatory disease associated with specific autoantibodies.Anti-leucine-rich glioma inactivated 1(anti-LGI1)encephalitis is a rare but treatable type of AE discovered in recent years.Alzheimer’s disease(AD)is a degenerative brain disease and the most common cause of dementia.AD may undergo a series of pathological physiological changes in brain tissue 20 years before the onset of typical symptoms.The stage of mild cognitive impairment(MCI)that occurs during this process,known as MCI due to AD,is the earliest stage with clinical symptoms.MCI is typically categorized into two subtypes:Amnestic MCI(aMCI)and non-aMCI.CASE SUMMARY This report describes a patient with rapid cognitive impairment,diagnosed with anti-LGI1 antibody-mediated AE and aMCI,and treated at Peking University Shenzhen Hospital in March 2023.The patient was hospitalized with acute memory decline for more than 3 months.Both the cerebrospinal fluid and serum were positive for anti-LGI1 antibodies,biomarkers of AD coexisting in the patient’s cerebrospinal fluid.Following combination treatment with immunoglobulin therapy and glucocorticoid,plus inhibition of acetylcholinesterase,the patient’s cognitive function significantly improved.Throughout the 3-month follow-up period,a sustained improvement in cognitive function was observed.The results of serum anti-LGI1 antibody were negative.CONCLUSION This case has raised awareness of the possible interaction between AE and early AD(including MCI due to AD),and alerted clinicians to the possibility of concurrent rare and common diseases in patients presenting with cognitive impairment.
基金funded by the National Natural Science Foundation of China(Grant No.31801421)the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAAS-ASTIPIVFCAAS).
文摘Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.
基金financially supported by the National Key Research and Development Program(No.2023YFB3712403)the National Natural Science Foundation of China(Nos.52201112,52071066,U22A20106,and U22A20173).
文摘Recycling-oriented alloy design is a crucial part of material sustainability,as it reduces the need for raw material extraction and minimises environmental impact.This requires that scraps be reused or repurposed effectively,even when the scraps are co-mingled and have higher costs for further sorting and separation.In this work,we explore an alloy design concept by creating a compositionally flexible domain that can recycle multiple alloy grades and yet maintain relatively consistent properties across chemical variations.This is demonstrated through the Fe-Cr-Ni-Mn system to identify compositionally flexible austenitic stainless steels(CF-ASS)and accommodate the recycling of mixed austenitic stainless steel scraps.Alloys within the nominal composition spaces exhibit relatively consistent mechanical properties and corrosion resistance despite significant variations in different alloy compositions.We illustrate how we can utilise the compositionally flexible austenitic stainless steels to recycle mixed 200 and 300-series stainless steel and ferronickel scraps,demonstrating its practical viability.While this demonstration focuses on the stainless steel system,the underlying principles can be extended to other systems related to mixed scrap recycling.
基金financially supported by the Beijing Natural Science Foundation(No.L233016)。
文摘Polyaniline(PANi)hydrogels have a wide range of applications in artificial skin,flexible robotics,and movement monitoring.Nevertheless,limited by the modulus mismatch between rigid PANi and the soft hydrogel matrix,the high strength and toughness of the PANi hydrogel are mutually exclusive.Although the introduction of sacrificial bonds into the hydrogel network can alleviate this contradiction to a certain extent,it always causes pronounced energy hysteresis during hydrogel deformation.Inspired by the energy storage and release of macroscopic springs,in this work,we propose a molecular entanglement approach for the fabrication of PANi hydrogels featuring high toughness and low hysteresis,where flexible poly(ethylene glycol)(PEG)is entangled with chemically cross-linked poly(acrylic acid)(PAA)as a hydrogel matrix,and rigid PANi as a conductive filler.The resultant PAA/PEG/PANi hydrogel exhibited high mechanical properties(fracture strength of 0.75 MPa and toughness of 4.81 MJ·m^(-3))and a low energy dissipation ratio(28.2%when stretching to 300%).Moreover,the PAA/PEG/PANi hydrogel possesses a good electrical response to external forces and can be employed as a strain sensor to monitor human joint movements by producing specific electrical signals.This work provides a straightforward strategy for preparing tough conductive PANi hydrogels with low hysteresis,showing potential for the development of healthcare devices.
基金supported by the Program for Science and Technology Innovation Team in Zhejiang Province,China(Grant No.2021R01004)the Six Talent Peaks Project of Jiangsu Province,China(Grant No.2019-XCL-081)the Startup Funding of Ningbo University and Yongjiang Recruitment Project(Grant No.432200942).
文摘The exploration of topological phases remains a cutting-edge research frontier,driven by their promising potential for next-generation electronic and quantum technologies.In this work,we employ first-principles calculations and tightbinding modeling to systematically investigate the topological properties of freestanding two-dimensional(2D)honeycomb Bi,HgTe,and Al_(2)O_(3)(0001)-supported HgTe.Remarkably,all three systems exhibit coexistence of intrinsic first-and higher-order topological insulator states,induced by spin-orbit coupling(SOC).These states manifest as topologically protected gapless edge states in one-dimensional(1D)nanoribbons and symmetry-related corner states in zero-dimensional(0D)nanoflakes.Furthermore,fractional electron charges may accumulate at the corners of armchair-edged nanoflakes.Among these materials,HgTe/Al_(2)O_(3)(0001)is particularly promising due to its experimentally feasible atomic configuration and low-energy corner states.Our findings highlight the importance of exploring higher-order topological phases in quantum spin Hall insulators and pave the way for new possibilities in device applications.
基金supported by the Li Ka Shing Foundation Cross Research Project(No.2020LKSFG02D)the Guangdong Basic and Applied Basic Research Foundation(Nos.2021A1515011285,2022A1515110566,2023A151501221574 and 2019A1515010800)+2 种基金Major Project under the Science and Technology Development Scheme of Guangdong Province(Nos.210715106900918 and[2020]53-129)the Shantou Science and Technology Plan Medical and Health Category Project(No.211114216492935)the 2022 Guangdong Science and Technology Innovation Strategy Special Fund for University Students(Science and Technology Innovation Cultivation)Project(No.pdjh2022a0186).
文摘Total hip arthroplasty(THA)has limitations in grinding angles,prosthesis placements,and thickness variations.THA robotics offer promise but encounter challenges like manual control of the robotic arm for precise positioning and potential over-grinding when controlled manually.This paper presents a THA surgical robot system with automatic positioning and automatic grinding and filing functions.It achieves precise positioning during the surgery by using the singular value decomposition of initial value screening and sliding mode control(SMC),and ensures uniformity,stability and controlled filing thickness through the designed end grinding and filing actuator system.It has been verified experimentally that the average position errors in the x,y,and z directions are 0.692 mm,0.512 mm,and 0.66 mm respectively,and the Euclidean distance error is 1.322 mm.The average angle error is less than 1.136°.The end effector can perform automatic grinding according to the predetermined planning value within the safe force threshold of 30 N.This THA surgical robot system can meet the requirements of the hip replacement surgery for the accuracy,driving ability and robustness of the system.
