Photocatalytic water splitting for hydrogen evolution reaction(HER)has emerged as one of the most promising approaches for solar energy utilization.Porous easily functionalized metal-organic framework(MOF)represents a...Photocatalytic water splitting for hydrogen evolution reaction(HER)has emerged as one of the most promising approaches for solar energy utilization.Porous easily functionalized metal-organic framework(MOF)represents a rising crystalline material for photocatalytic application.Yet,most MOFs still face challenges like chemical instability in solution media,no photosensitization,and ambiguous active sites.Herein,thiol-dense Hf-or Zr-based porous frameworks(Hf-,Zr-TBAPy-8SH)were prepared as platforms for facile construction of HER active sites by anchoring transition metal(TM)ions as well as forming heterojunction with nanoscale semiconductor(CdS).The highest HER rate of 8.15mmol g^(-1) h^(-1) by Co(Ⅱ)-loaded Hf-based composite highlight(1)[S^(-)-Co]motifs as competent HER site,(2)match heterojunction outweighing traditional photosensitizer-mediated HER,(3)regulating electron density of metal-oxo cluster as a way to harness HER activity.This study firstly demonstrates synergy of Hf-oxo clusters,thiol functionalities and heterojunction as an easy yet controllable strategy to form integrated photocatalyst.展开更多
Catalytic regeneration is a key approach to solving high energy consumption issues in the amine-based CO_(2)absorption method.Previous studies have shown that loaded acid sites(such as SO_(4)^(2-))are beneficial for p...Catalytic regeneration is a key approach to solving high energy consumption issues in the amine-based CO_(2)absorption method.Previous studies have shown that loaded acid sites(such as SO_(4)^(2-))are beneficial for promoting low-temperature CO_(2)-rich amine regeneration,but their weak binding strength to the support results in limited catalyst life.Herein,we proposed an advanced catalyst modification strategy to maintain the active hydroxyl group(Zr-OH-Fe)via actively transferring electrons on the surface of FeZrO_(x)nano-heterojunction.Combining in situ DRIFTS and DFT calculations,we revealed that the ZrOH-Fe at the ZrO_(2)-Fe_(2)O_(3)heterointerfaces exhibit enhanced proton-donating ability,with deprotonation energy reduced from 2.94 to 2.61 eV compared to Zr-OH(which should be called inert hydroxyl group).This improvement favors the rate-determining proton transfer step from RNH_(3)^(+)to RNHCOO^(-).Surprisingly,it increased the CO_(2)desorption rate by 10.5 times and reduced the energy consumption by 43.6%during amine regeneration.This work offers a practical strategy for improving the performance of lowtemperature CO_(2)-rich amine regeneration catalysts,and the low-cost recyclability of amine used in CO_(2)capture.展开更多
Background Inflammatory bowel disease causes intestinal structural damage,impairs gut function,hinders animal growth and development,and reduces farming efficiency.Previous studies demonstrated that lactate alleviates...Background Inflammatory bowel disease causes intestinal structural damage,impairs gut function,hinders animal growth and development,and reduces farming efficiency.Previous studies demonstrated that lactate alleviates dextran sulfate sodium(DSS)-induced inflammation and mitigates weight loss by enhancing intestinal barrier functions.However,the mechanisms underlying lactate-mediated protection of the intestinal epithelial barrier remain unclear.This study aimed to explore the protective effect of lactate on intestinal barrier damage in colitis piglets and the possible underlying mechanisms through in vivo and in vitro experiments.Methods A total of 6021-day-old weaned female piglets were randomly assigned into three groups based on weight:the control group(basal diet with physiological saline gavage),the DSS group(basal diet with 5%DSS gavage),and the DSS+LA group(2%lactate diet with 5%DSS gavage).There were 10 replicates per treatment,with 2 piglets per replicate.Jejunal morphology was assessed via hematoxylin and eosin staining,while Western blotting quantified the protein levels of proliferation markers,including cluster of differentiation 24(CD24),cyclin D1,and wingless/integrated(Wnt)/β-catenin signaling components.In vitro,0.08%DSS and 2–32 mmol/L sodium lactate-treated intestinal porcine epithelial cell line-J2(IPEC-J2)cells(n=4)were assessed for viability(Cell Counting Kit-8 assay),apoptosis(flow cytometry),and proliferation parameters,including cell cycle analysis and Leucine-rich repeat-containing G-protein coupled receptor 5(Lgr5+)stem cell quantification.Results In vivo,DSS administration induced jejunal villus shortening(P<0.05),downregulated protein levels of CD24,cyclin D1,casein kinase 1(CK1),and dishevelled-2(DVL2)(P<0.05).In vitro,DSS promoted apoptosis,inhibited proliferation,diminished the Lgr5+cell populations(P<0.05),and reduced S-phase cell proportions(P<0.05).Conversely,lactate supplementation ameliorated DSS-induced villus atrophy(P<0.05),restored CD24,cyclin D1,CK1,and DVL2 protein levels(P<0.05).Furthermore,in vitro,sodium lactate attenuated DSS-induced apoptosis(P<0.05),enhanced IPEC-J2 proliferation(P<0.05),expanded Lgr5+cells(P<0.05),and increased S-phase progression(P<0.05).Conclusions In summary,lactate ameliorated intestinal barrier damage in DSS-induced colitis by activating the Wnt/β-catenin pathway and restoring the balance between epithelial cell proliferation and apoptosis.This study provides novel mechanistic evidence supporting lactate's therapeutic potential for IBD management.展开更多
Background Diarrhea remains a major health concern in both young animals and humans.Prevotella spp.,a dominant commensal genus in the healthy porcine gut,becomes increasingly abundant following weaning,suggesting a po...Background Diarrhea remains a major health concern in both young animals and humans.Prevotella spp.,a dominant commensal genus in the healthy porcine gut,becomes increasingly abundant following weaning,suggesting a potential role during this critical transitional period.However,its involvement in post-weaning diarrhea remains poorly understood.Here,we aim to elucidate the role and underlying mechanisms of Prevotella in alleviating diarrhea in weaned piglets.Results To model unsanitary housing conditions,piglets were housed in uncleaned pens containing residual fecal matter from previous occupants and exposed to cold stress by maintaining the ambient temperature at 19℃,below the optimal 28℃.Under these conditions,piglets were orally administered either a blank medium(CON,n=10×2)or Prevotella copri at 1×10^(8)CFU(Pc,n=10×2)on d 1,3,and 5.After 28 d,cold stress induced a diarrhea incidence of 33.45%in the CON group,while P.copri supplementation significantly reduced the diarrhea rate to 19.73%.Treatment with P.copri markedly improved intestinal morphology in the small intestine,decreased serum levels of lipopolysaccharide(LPS)and intestinal fatty acid-binding protein(i-FABP),and enhanced total antioxidant capacity(T-AOC)and catalase(CAT)activity.Quantitative PCR and 16S rRNA gene sequencing revealed that P.copri significantly increased the colonic abundance of Prevotella,reshaping both the composition and functional profile of the gut microbiota.Moreover,P.copri enhanced the modularity and robustness of microbial ecological networks.Untargeted metabolomic profiling of colonic contents revealed a significant enrichment of metabolites involved in the arachidonic acid pathway following P.copri supplementation.In parallel,untargeted metabolomics of P.copri culture supernatants identified differential metabolic pathways including metabolic pathways,biosynthesis of secondary metabolites,and biosynthesis of antibiotics.In vitro assays demonstrated that P.copri-derived metabolites inhibited the growth of three common porcine intestinal pathogens.Furthermore,both P.copri metabolites and arachidonic acid enhanced intestinal barrier integrity and suppressed TNF-α-induced inflammation and apoptosis in Caco-2 cells through activation of the AHR–Nrf2 signaling pathway.Conclusions These findings highlight the role of P.copri in maintaining gut homeostasis and provide new insights into microbiota-based interventions for early-life intestinal disorders.展开更多
Background： As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of th...Background： As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of the effects of benzoic acid on jejunal digestive physiology, especially the antioxidant capacity and mucosal glucagon-like peptide2（GLP-2） concentrations is lacking.Methods： A total of 20 barrows [Duroc ×（Yorkshire × Landrace）] with an average body weight（BW） of 18.75 ± 0.2 kg were used in a 14-d trial to determine the potential mechanisms of benzoic acid supplementation on the performance,nutrient digestibility and jejunal digestive physiology in young pigs. All pigs were randomly allotted to 1 of 2 diets supplemented with 0 or 5000 mg/kg benzoic acid.Results： Relative to the control, benzoic acid supplementation increased the average daily feed intake（ADFI）, and average daily gain（ADG） in young pigs（P 〈 0.05）, improved the apparent total tract digestibility of dry matter（DM）,crude protein（CP）, ether extract（EE）, gross energy（GE） and crude ash（P 〈 0.05）, and enhanced the activities of trypsin,lipase and amylase in the jejunum（P 〈 0.05）. Similarly, relative to the control, supplementing benzoic acid in the diet resulted in a trend to reduce the p H values of the digesta（P = 0.06）, decreased crypt depth and increased the villus height to crypt depth ratio（P 〈 0.05） in the jejunum of pigs. Finally, benzoic acid supplementation increased the mR NA expression and concentration of glucagon-like peptide 2 and the activities of glutathione peroxidase and superoxide dismutase in the jejunal mucosa of young pigs（P 〈 0.05）.Conclusions： In conclusion, supplementation with 5000 mg/kg benzoic acid improved the performance of young pigs through promoting nutrient digestion, improving jejunal antioxidant capacity, and maintaining the jejunal morphology in young pigs.展开更多
It is a big challenge to tune the structure and composition of carbon-based anode materials to increase the active sites by a green synthesis strategy for potassium ion batteries(PIBs).Herein,the N/F/S co-doped three-...It is a big challenge to tune the structure and composition of carbon-based anode materials to increase the active sites by a green synthesis strategy for potassium ion batteries(PIBs).Herein,the N/F/S co-doped three-dimensional(3D)interconnected carbon nanosheets(NFS-CNSs)were synthesized from coal tar pitch(CTP)through a green and low-temperature treatment process for the first time.The as-obtained NFS-CNS600 features 3D interconnected ultra-thin carbon nanosheets with abundant active sites,tunable N/F/S species,and enlarged carbon interlayer spacing.The density functional theory calculation results demonstrate that NFS-CNSs exhibit the highest electron density and most negative K^(+)adsorption energy(-0.59 eV)compared to single or double-atom doping,thereby enhancing the storage performance of K+.As an anode for PIBs,the NFS-CNS600 exhibits good cycle stability(98.2%capacity retention after 200 cycles at 0.2 A g^(-1)),high capacity(409.1 mAh g^(-1) at 0.05 A g^(-1))and rate performance(179.5 mAh g^(-1) at 5 A g^(-1)).Besides,the NFS-CNS600 anode also displays outstanding sodium storage performance.This work offers a green strategy to synthesize CTP-based anode materials from coal chemical by-products for high-performance PIBs.展开更多
Background Oxidative stress significantly impacts growth performance and liver function in piglets.Ferulic acid(FA)works as an antioxidant,however,the role and mechanism of FA in the regulation of diquat-induced oxida...Background Oxidative stress significantly impacts growth performance and liver function in piglets.Ferulic acid(FA)works as an antioxidant,however,the role and mechanism of FA in the regulation of diquat-induced oxidative stress in piglets are less known.This study was designed to investigate the effects of FA on growth performance and antioxi-dant capacity in piglets with diquat challenge.Methods Thirty-two healthy DLY(Duroc×Landrace×Yorkshire)piglets(13.24±0.19 kg)were randomly divided into one of two diets including 0 or 4 g/kg FA for 14 d.On d 15,all pigs were intraperitoneally injected diquat or sterile saline.Results Dietary supplementation with ferulic acid(FA)significantly improved the average daily gain(ADG)and decreased feed-gain ratio(F/G)of piglets.Here,dietary FA supplementation reduced serum aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT)activities in diquat challenged piglets.Furthermore,diquat infusion increased reactive oxygen radicals(ROS)level in liver,decreased the activities of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and increased malondialdehyde(MDA)con-tent in the liver and serum.Supplementation with FA significantly increased T-AOC and T-SOD activities and decreased MDA and ROS levels.FA down-regulated gene and protein expression of Keap1,and up-regulated protein expression of Nrf2 and HO-1 in the liver of piglets with diquat challenge.Importantly,diquat challenge increased the ratio of late apoptosis,increased serum levels of IL-1β,IL-18 and lactate dehydrogenase(LDH),and up-regulated pyroptosis-related genes in the liver.FA supplementation reduced the ratio of late apoptosis and down-regulated mRNA expression of Caspase-1.Accordingly,FA addition reduced concentration of IL-1β,IL-18,and LDH under diquat challenge.Conclusions Diquat-induced oxidative stress reduced growth performance and impaired liver function in piglets.Dietary FA supplementation enhanced the antioxidant capacity and reduced the degree of hepatocyte pyroptosis,thereby alleviating the oxidative damage in the liver and mitigating the impact of diquat on growth performance of piglets.展开更多
BACKGROUND Intestinal barrier dysfunction is a prevalent and varied manifestation of acute pancreatitis(AP).Molecular mechanisms underlying the early intestinal barrier in AP remain poorly understood.AIM To explore th...BACKGROUND Intestinal barrier dysfunction is a prevalent and varied manifestation of acute pancreatitis(AP).Molecular mechanisms underlying the early intestinal barrier in AP remain poorly understood.AIM To explore the biological processes and mechanisms of intestinal injury associated with AP,and to find potential targets for early prevention or treatment of intestinal barrier injury.METHODS This study utilized single-cell RNA sequencing of the small intestine,alongside in vitro and in vivo experiments,to examine intestinal barrier function homeostasis during the early stages of AP and explore involved biological processes and potential mechanisms.RESULTS Seventeen major cell types and 33232 cells were identified across all samples,including normal,AP1(4x caerulein injections,animals sacrificed 2 h after the last injection),and AP2(8x caerulein injections,animals sacrificed 4 h after the last injection).An average of 980 genes per cell was found in the normal intestine,compared to 927 in the AP1 intestine and 1382 in the AP2 intestine.B cells,dendritic cells,mast cells(MCs),and monocytes in AP1 and AP2 showed reduced numbers compared to the normal intestine.Enterocytes,brush cells,enteroendocrine cells,and goblet cells maintained numbers similar to the normal intestine,while cytotoxic T cells and natural killer(NK)cells increased.Enterocytes in early AP exhibited elevated programmed cell death and intestinal barrier dysfunction but retained absorption capabilities.Cytotoxic T cells and NK cells showed enhanced pathogen-fighting abilities.Activated MCs,secreted chemokine(C-C motif)ligand 5(CCL5),promoted neutrophil and macrophage infiltration and contributed to barrier dysfunction.CONCLUSION These findings enrich our understanding of biological processes and mechanisms in AP-associated intestinal injury,suggesting that CCL5 from MCs is a potential target for addressing dysfunction.展开更多
Oxygen evolution reaction(OER),a critical half-reaction in photocatalytic overall water splitting for producing hydrogen,is a key step toward sustainable energy conversion.Conventional photocatalysts often suffer from...Oxygen evolution reaction(OER),a critical half-reaction in photocatalytic overall water splitting for producing hydrogen,is a key step toward sustainable energy conversion.Conventional photocatalysts often suffer from limited light absorption and rapid charge recombination,hindering their further applications.To address these challenges,we have designed and synthesized a novel series of self-sensitized metal-organic frameworks(MOFs),Fe_(2)MCDDB(M=Ni,Mn,or Co).By incorporating photosensitive ligands,we have achieved efficient charge separation and promoted the transfer of photogenerated electrons to the active metal sites for water oxidation.Among the series,Fe_(2)NiCDDB exhibits exceptional OER activity,achieving an oxygen evolution rate of 125.3μmol g^(−1)h^(−1)under visible light irradiation.Experimental and theoretical results reveal that the optimized electronic structure and prolonged excited-state lifetime of Fe_(2)NiCDDB contribute to its enhanced catalytic performance.This work provides a promising strategy for designing two-in-one MOF photocatalysts for water oxidation.展开更多
Large-scale and heavily jointed rocks have inherent planes of anisotropy and secondary structural planes,such as dominant joint sets and random fractures,which result in significant differences in their failure mechan...Large-scale and heavily jointed rocks have inherent planes of anisotropy and secondary structural planes,such as dominant joint sets and random fractures,which result in significant differences in their failure mechanism and deformation behavior compared to other rock types.To address this issue,inherent anisotropic rocks with large-scale and dense joints are considered to be composed of the rock matrix,inherent planes of anisotropy,and secondary structural planes.Then a new implicit continuum model called LayerDFN is developed based on the crack tensor and damage tensor theories to characterize the mechanical properties of inherent anisotropic rocks.Furthermore,the LayerDFN model is implemented in the FLAC3D software,and a series of numerical results for typical example problems is compared with those obtained from the 3DEC,the analytical solutions,similar classical models,laboratory uniaxial compression tests,and field rigid bearing plate tests.The results demonstrate that the LayerDFN model can effectively capture the anisotropic mechanical properties of inherent anisotropic rocks,and can quantitatively characterize the damaging effect of the secondary structural planes.Overall,the numerical method based on the LayerDFN model provides a comprehensive and reliable approach for describing and analyzing the behavior of inherent anisotropic rocks,which will provide valuable insights for engineering design and decision-making processes.展开更多
To address the issues of unclear carbon responsibility attribution,insufficient renewable energy absorption,and simplistic carbon trading mechanisms in integrated energy systems,this paper proposes an electricheat-hyd...To address the issues of unclear carbon responsibility attribution,insufficient renewable energy absorption,and simplistic carbon trading mechanisms in integrated energy systems,this paper proposes an electricheat-hydrogen integrated energy system(EHH-IES)optimal scheduling model considering carbon emission stream(CES)and wind-solar accommodation.First,the CES theory is introduced to quantify the carbon emission intensity of each energy conversion device and transmission branch by defining carbon emission rate,branch carbon intensity,and node carbon potential,realizing accurate tracking of carbon flow in the process of multi-energy coupling.Second,a stepped carbon pricing mechanism is established to dynamically adjust carbon trading costs based on the deviation between actual carbon emissions and initial quotas,strengthening the emission reduction incentive.Finally,a lowcarbon economic dispatch model is constructed with the objectives of minimizing operation cost,carbon trading cost,wind-solar curtailment penalty cost,and energy loss.Simulation results show that compared with the traditional economic dispatch scheme 3,the proposed schemel reduces carbon emissions by 53.97%and wind-solar curtailment by 68.89%with a 16.10%increase in total cost.This verifies that the model can effectively improve clean energy utilization and reduce carbon emissions,achieving low-carbon economic operation of EHH-IES,with CES theory ensuring precise carbon flow tracking across multi-energy links.展开更多
Background Inflammatory bowel disease is a significant health concern for both humans and large-scale farm animals.In the quest for effective alternatives to antibiotics,next-generation probiotics(NGPs)have emerged as...Background Inflammatory bowel disease is a significant health concern for both humans and large-scale farm animals.In the quest for effective alternatives to antibiotics,next-generation probiotics(NGPs)have emerged as a promising option.The genus Blautia presents a rich source of potential NGP strains.Here we successfully isolated Blautia hominis LYH1 strain from the intestines of healthy weaned piglets and characterized its biological traits.Its antiinflammatory activity was then assessed using macrophages,while its protective effects against colitis and gut barrier damage were validated in a DSS-induced mouse colitis model.Results B.hominis LYH1 displayed typical characteristics of an obligate anaerobe,including non-hemolytic and nonmotile features,and a genome enriched with carbohydrate-active enzyme genes.It produced metabolites with antibiotic-like compounds,demonstrating antimicrobial activity against Escherichia coli.In vitro,B.hominis LYH1 effectively inhibited pathogen replication in macrophages,reducing cellular infections and alleviating inflammatory damage.In vivo,oral administration of B.hominis LYH1 or its metabolites significantly mitigated DSS-induced colitis in mice by suppressing pro-inflammatory cytokines,inhibiting T-lymphocyte activation,and enhancing short-chain fatty acid production.Conclusions Our findings underscore B.hominis LYH1’s potential as a NGP for maintaining gut health and combating intestinal inflammation.These findings offer valuable insights into the development of antibiotic alternatives and innovative strategies for preventing and treating enteritis in both agricultural and medical settings.展开更多
Hepatoid adenocarcinoma of the stomach (HAS)is an extremely rare and unique gastric malignancy.The present study aimed to examine the relevance of the clinicopathological characteristics of HAS with patient prognosis....Hepatoid adenocarcinoma of the stomach (HAS)is an extremely rare and unique gastric malignancy.The present study aimed to examine the relevance of the clinicopathological characteristics of HAS with patient prognosis.We retrospectively reviewed clinical data of 34 HAS patients treated at our institution between January 2010 and December 2016,as well as 294 cases reported prior to 2017 in research databases.Among these patients,45.6%(115/252)had lesions in the gastric antrum and 77.0%(235/305)were male.Elevated levels of serum alpha-fetoprotein (AFP)were detected in most patients(75/93,80.6%).Vascular invasion(199/286,69.6%),lymph node metastasis (222/283,78.4%),and preoperative distant metastasis (121/328,36.9%)were commonly observed.The 5-year disease-free survival (DFS)and disease-specific survival (DSS) were 20.7%and 29.2%,respectively.DFS and DSS of patients receiving neoadjuvant therapy were significantly higher than those of patients receiving postoperative adjuvant therapy [DFS:P<0.001, hazard ratio (HR)=-1.831,95%confidence interval (CI):0.060-0.429;DSS:P<0.001,HR=-2.185, 95%CI:0.032-0.401].In conclusion,HAS exhibits distinct clinicopathological characteristics and a strikingly worse prognosis when compared with common gastric cancer.Complete surgery,early pTNM stage,and adjuvant therapy may predict a more favorable prognosis.Neoadjuvant therapy is strongly recommended for patients with lymph node metastasis or/and preoperative distant metastasis.展开更多
Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)...Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity.展开更多
Background: Alginate oligosaccharide(AOS), produced from alginate by alginate lyase-mediated depolymerisation, is a potential substitute for antibiotics and possesses growth-enhancing effects. Nevertheless, the mechan...Background: Alginate oligosaccharide(AOS), produced from alginate by alginate lyase-mediated depolymerisation, is a potential substitute for antibiotics and possesses growth-enhancing effects. Nevertheless, the mechanisms by which AOS regulates porcine growth remain to be elucidated. Therefore, we investigated the AOS-mediated changes in the growth performance of weaned pigs by determining the intestinal morphology, barrier function,as well as epithelium apoptosis.Methods: Twenty-four weaned pigs were distributed into two groups(n = 12) and received either a basal diet(control group) or the same diet supplemented with 100 mg/kg AOS. On d 15, D-xylose(0.1 g/kg body weight)was orally administrated to eight randomly selected pigs per treatment, and their serum and intestinal mucosa samples were collected 1 h later.Results: Our results showed that inclusion of AOS in the diet for 2 wk increased(P < 0.05) the average daily body weight gain in weaned pigs. Notably, AOS supplementation ameliorated the intestinal morphology and barrier function, as suggested by the enhanced(P < 0.05) intestinal villus height, secretory immunoglobulin A content and goblet cell counts. Compared to the control group, AOS ingestion both decreased(P < 0.05) the total apoptotic percentage and increased(P < 0.05) the proportion of S phase in the intestinal epithelial cells. Furthermore, AOS not only up-regulated(P < 0.05) the B-cell lymphoma-2(BCL2) transcriptional level but also down-regulated(P < 0.05) the B-cell lymphoma-2-associated X protein(BAX), cysteinyl aspartate-specific proteinase-3(caspase-3) and caspase-9 transcriptional levels in the small intestine.Conclusions: In summary, this study provides evidence that supplemental AOS beneficially affects the growth performance of weaned pigs, which may result from the improved intestinal morphology and barrier function,as well as the inhibited enterocyte death, through reducing apoptosis via mitochondria-dependent apoptosis.展开更多
Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diq...Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0A5% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCI solution or diquat （10 mg/kg body weight）. During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain （P = 0.09） and average daily feed intake （P = 0.08）, and decreased the feed efficiency （P 〈 0.05） of piglets. The growth performance was decreased by diquat injection （P 〈 0.05）. Diquat injection also decreased the activities of the superoxide dismutase （SOD） and glutathione peroxidase （GPx） in the plasma and liver （P 〈 0.05）, increased plasma malondialdehyde （MDA） （P 〈 0.05） and urea nitrogen （P 〈 0.05） concentrations, and enhanced MDA concentration （P = 0.09） and tryptophan 2,3-dioxygenase （TDO） activity （P = 0.07） in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA （P = 0.06） concentration and the activities of SOD （P = 0.09） and GPx （P = 0.05） of the liver, and plasma urea nitrogen （P = 0.06） concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection （P 〈 0.05）. These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.展开更多
Background Genomic selection involves choosing as parents those elite individuals with the higher genomic estimated breeding values(GEBV)to accelerate the speed of genetic improvement in domestic animals.But after mul...Background Genomic selection involves choosing as parents those elite individuals with the higher genomic estimated breeding values(GEBV)to accelerate the speed of genetic improvement in domestic animals.But after multi-generation selection,the rate of inbreeding and the occurrence of homozygous harmful alleles might increase,which would reduce performance and genetic diversity.To mitigate the above problems,we can utilize genomic mating(GM)based upon optimal mate allocation to construct the best genotypic combinations in the next generation.In this study,we used stochastic simulation to investigate the impact of various factors on the efficiencies of GM to optimize pairing combinations after genomic selection of candidates in a pig population.These factors included:the algorithm used to derive inbreeding coefficients;the trait heritability(0.1,0.3 or 0.5);the kind of GM scheme(focused average GEBV or inbreeding);the approach for computing the genomic relationship matrix(by SNP or runs of homozygosity(ROH)).The outcomes were compared to three traditional mating schemes(random,positive assortative or negative assortative matings).In addition,the performance of the GM approach was tested on real datasets obtained from a Large White pig breeding population.Results Genomic mating outperforms other approaches in limiting the inbreeding accumulation for the same expected genetic gain.The use of ROH-based genealogical relatedness in GM achieved faster genetic gains than using relatedness based on individual SNPs.The GROH-based GM schemes with the maximum genetic gain resulted in 0.9%-2.6%higher rates of genetic gainΔG,and 13%-83.3%lowerΔF than positive assortative mating regardless of heritability.The rates of inbreeding were always the fastest with positive assortative mating.Results from a purebred Large White pig population,confirmed that GM with ROH-based GRM was more efficient than traditional mating schemes.Conclusion Compared with traditional mating schemes,genomic mating can not only achieve sustainable genetic progress but also effectively control the rates of inbreeding accumulation in the population.Our findings demonstrated that breeders should consider using genomic mating for genetic improvement of pigs.展开更多
With a large number of researches being conducted on two?dimen?sional(2D)materials,their unique properties in optics,electrics,mechanics,and magnetics have attracted increasing attention.Accordingly,the idea of combin...With a large number of researches being conducted on two?dimen?sional(2D)materials,their unique properties in optics,electrics,mechanics,and magnetics have attracted increasing attention.Accordingly,the idea of combining distinct functional 2D materials into heterostructures naturally emerged that pro?vides unprecedented platforms for exploring new physics that are not accessible in a single 2D material or 3D heterostructures.Along with the rapid development of controllable,scalable,and programmed synthesis techniques of high?quality 2D heterostructures,various heterostructure devices with extraordinary performance have been designed and fabricated,including tunneling transistors,photodetectors,and spintronic devices.In this review,we present a summary of the latest progresses in fabrications,properties,and applications of di erent types of 2D heterostruc?tures,followed by the discussions on present challenges and perspectives of further investigations.展开更多
Background:Sialyllactose(SL)is one of the most abundant oligosaccharides present in porcine breast milk.However,little is known about its effect on growth performance and intestinal health in weaned pigs.This study wa...Background:Sialyllactose(SL)is one of the most abundant oligosaccharides present in porcine breast milk.However,little is known about its effect on growth performance and intestinal health in weaned pigs.This study was conducted to explore the protective effect of SL on intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli(ETEC)challenge.Methods:Thirty-two pigs were randomly divided into four treatments.Pigs fed with a basal diet or basal diet containing SL(5.0 g/kg)were orally infused with ETEC or culture medium.Results:SL supplementation elevated the average daily gain(ADG)and feed efficiency in the ETEC-challenged pigs(P<0.05).SL also improved the digestibilities of dry matter(DM),gross energy(GE),and ash in non-challenged pigs(P<0.05).Moreover,SL not only elevated serum concentrations of immunoglobulins(IgA,IgG,and IgM),but also significantly decreased the serum concentrations of inflammatory cytokines(TNF-α,IL-1β,and IL-6)upon ETEC challenge(P<0.05).Interestingly,SL increased the villus height,the ratio of villus height to crypt depth(V:C),and the activities of mucosal sucrase and maltase in the jejunum and ileum(P<0.05).SL also elevated the concentrations of microbial metabolites(e.g.acetic acid,propanoic acid,and butyric acid)and the abundance of Lactobacillus,Bifidobacterium,and Bacillus in the cecum(P<0.05).Importantly,SL significantly elevated the expression levels of jejunal zonula occludins-1(ZO-1),occluding,and fatty acid transport protein-4(FATP4)in the ETEC-challenged pigs(P<0.05).Conclusions:SL can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge,which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins,as well as improved intestinal epithelium functions and microbiota.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22371054,22301045)the Foundation of Basic and Applied Basic Research of Guangdong Province(Nos.2020B1515120024,2024A1515012801)Science and Technology Planning Project of Guangdong Province(Nos.2021A0505030066,2023A0505050164).
文摘Photocatalytic water splitting for hydrogen evolution reaction(HER)has emerged as one of the most promising approaches for solar energy utilization.Porous easily functionalized metal-organic framework(MOF)represents a rising crystalline material for photocatalytic application.Yet,most MOFs still face challenges like chemical instability in solution media,no photosensitization,and ambiguous active sites.Herein,thiol-dense Hf-or Zr-based porous frameworks(Hf-,Zr-TBAPy-8SH)were prepared as platforms for facile construction of HER active sites by anchoring transition metal(TM)ions as well as forming heterojunction with nanoscale semiconductor(CdS).The highest HER rate of 8.15mmol g^(-1) h^(-1) by Co(Ⅱ)-loaded Hf-based composite highlight(1)[S^(-)-Co]motifs as competent HER site,(2)match heterojunction outweighing traditional photosensitizer-mediated HER,(3)regulating electron density of metal-oxo cluster as a way to harness HER activity.This study firstly demonstrates synergy of Hf-oxo clusters,thiol functionalities and heterojunction as an easy yet controllable strategy to form integrated photocatalyst.
基金financially supported by the National Natural Science Foundation of China(52222005,52100133)the Key R&D Program of Yunnan Province(202303AC100008)。
文摘Catalytic regeneration is a key approach to solving high energy consumption issues in the amine-based CO_(2)absorption method.Previous studies have shown that loaded acid sites(such as SO_(4)^(2-))are beneficial for promoting low-temperature CO_(2)-rich amine regeneration,but their weak binding strength to the support results in limited catalyst life.Herein,we proposed an advanced catalyst modification strategy to maintain the active hydroxyl group(Zr-OH-Fe)via actively transferring electrons on the surface of FeZrO_(x)nano-heterojunction.Combining in situ DRIFTS and DFT calculations,we revealed that the ZrOH-Fe at the ZrO_(2)-Fe_(2)O_(3)heterointerfaces exhibit enhanced proton-donating ability,with deprotonation energy reduced from 2.94 to 2.61 eV compared to Zr-OH(which should be called inert hydroxyl group).This improvement favors the rate-determining proton transfer step from RNH_(3)^(+)to RNHCOO^(-).Surprisingly,it increased the CO_(2)desorption rate by 10.5 times and reduced the energy consumption by 43.6%during amine regeneration.This work offers a practical strategy for improving the performance of lowtemperature CO_(2)-rich amine regeneration catalysts,and the low-cost recyclability of amine used in CO_(2)capture.
基金funded by the Sichuan Science and Technology Program(2021ZDZX0009)the earmarked fund from the National Natural Science Foundation of China(31972577)。
文摘Background Inflammatory bowel disease causes intestinal structural damage,impairs gut function,hinders animal growth and development,and reduces farming efficiency.Previous studies demonstrated that lactate alleviates dextran sulfate sodium(DSS)-induced inflammation and mitigates weight loss by enhancing intestinal barrier functions.However,the mechanisms underlying lactate-mediated protection of the intestinal epithelial barrier remain unclear.This study aimed to explore the protective effect of lactate on intestinal barrier damage in colitis piglets and the possible underlying mechanisms through in vivo and in vitro experiments.Methods A total of 6021-day-old weaned female piglets were randomly assigned into three groups based on weight:the control group(basal diet with physiological saline gavage),the DSS group(basal diet with 5%DSS gavage),and the DSS+LA group(2%lactate diet with 5%DSS gavage).There were 10 replicates per treatment,with 2 piglets per replicate.Jejunal morphology was assessed via hematoxylin and eosin staining,while Western blotting quantified the protein levels of proliferation markers,including cluster of differentiation 24(CD24),cyclin D1,and wingless/integrated(Wnt)/β-catenin signaling components.In vitro,0.08%DSS and 2–32 mmol/L sodium lactate-treated intestinal porcine epithelial cell line-J2(IPEC-J2)cells(n=4)were assessed for viability(Cell Counting Kit-8 assay),apoptosis(flow cytometry),and proliferation parameters,including cell cycle analysis and Leucine-rich repeat-containing G-protein coupled receptor 5(Lgr5+)stem cell quantification.Results In vivo,DSS administration induced jejunal villus shortening(P<0.05),downregulated protein levels of CD24,cyclin D1,casein kinase 1(CK1),and dishevelled-2(DVL2)(P<0.05).In vitro,DSS promoted apoptosis,inhibited proliferation,diminished the Lgr5+cell populations(P<0.05),and reduced S-phase cell proportions(P<0.05).Conversely,lactate supplementation ameliorated DSS-induced villus atrophy(P<0.05),restored CD24,cyclin D1,CK1,and DVL2 protein levels(P<0.05).Furthermore,in vitro,sodium lactate attenuated DSS-induced apoptosis(P<0.05),enhanced IPEC-J2 proliferation(P<0.05),expanded Lgr5+cells(P<0.05),and increased S-phase progression(P<0.05).Conclusions In summary,lactate ameliorated intestinal barrier damage in DSS-induced colitis by activating the Wnt/β-catenin pathway and restoring the balance between epithelial cell proliferation and apoptosis.This study provides novel mechanistic evidence supporting lactate's therapeutic potential for IBD management.
基金supported by National Natural Science Foundation of China(31730091,32372900,32072743)Natural Science Foundation of Sichuan Province(2023NSFSC0237)Major Science and Technology Projects in Sichuan Province(2021ZDZX0009)。
文摘Background Diarrhea remains a major health concern in both young animals and humans.Prevotella spp.,a dominant commensal genus in the healthy porcine gut,becomes increasingly abundant following weaning,suggesting a potential role during this critical transitional period.However,its involvement in post-weaning diarrhea remains poorly understood.Here,we aim to elucidate the role and underlying mechanisms of Prevotella in alleviating diarrhea in weaned piglets.Results To model unsanitary housing conditions,piglets were housed in uncleaned pens containing residual fecal matter from previous occupants and exposed to cold stress by maintaining the ambient temperature at 19℃,below the optimal 28℃.Under these conditions,piglets were orally administered either a blank medium(CON,n=10×2)or Prevotella copri at 1×10^(8)CFU(Pc,n=10×2)on d 1,3,and 5.After 28 d,cold stress induced a diarrhea incidence of 33.45%in the CON group,while P.copri supplementation significantly reduced the diarrhea rate to 19.73%.Treatment with P.copri markedly improved intestinal morphology in the small intestine,decreased serum levels of lipopolysaccharide(LPS)and intestinal fatty acid-binding protein(i-FABP),and enhanced total antioxidant capacity(T-AOC)and catalase(CAT)activity.Quantitative PCR and 16S rRNA gene sequencing revealed that P.copri significantly increased the colonic abundance of Prevotella,reshaping both the composition and functional profile of the gut microbiota.Moreover,P.copri enhanced the modularity and robustness of microbial ecological networks.Untargeted metabolomic profiling of colonic contents revealed a significant enrichment of metabolites involved in the arachidonic acid pathway following P.copri supplementation.In parallel,untargeted metabolomics of P.copri culture supernatants identified differential metabolic pathways including metabolic pathways,biosynthesis of secondary metabolites,and biosynthesis of antibiotics.In vitro assays demonstrated that P.copri-derived metabolites inhibited the growth of three common porcine intestinal pathogens.Furthermore,both P.copri metabolites and arachidonic acid enhanced intestinal barrier integrity and suppressed TNF-α-induced inflammation and apoptosis in Caco-2 cells through activation of the AHR–Nrf2 signaling pathway.Conclusions These findings highlight the role of P.copri in maintaining gut homeostasis and provide new insights into microbiota-based interventions for early-life intestinal disorders.
基金financially supported by a China Pig Modern Industrial Technology System Grant (CARS-36)
文摘Background： As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of the effects of benzoic acid on jejunal digestive physiology, especially the antioxidant capacity and mucosal glucagon-like peptide2（GLP-2） concentrations is lacking.Methods： A total of 20 barrows [Duroc ×（Yorkshire × Landrace）] with an average body weight（BW） of 18.75 ± 0.2 kg were used in a 14-d trial to determine the potential mechanisms of benzoic acid supplementation on the performance,nutrient digestibility and jejunal digestive physiology in young pigs. All pigs were randomly allotted to 1 of 2 diets supplemented with 0 or 5000 mg/kg benzoic acid.Results： Relative to the control, benzoic acid supplementation increased the average daily feed intake（ADFI）, and average daily gain（ADG） in young pigs（P 〈 0.05）, improved the apparent total tract digestibility of dry matter（DM）,crude protein（CP）, ether extract（EE）, gross energy（GE） and crude ash（P 〈 0.05）, and enhanced the activities of trypsin,lipase and amylase in the jejunum（P 〈 0.05）. Similarly, relative to the control, supplementing benzoic acid in the diet resulted in a trend to reduce the p H values of the digesta（P = 0.06）, decreased crypt depth and increased the villus height to crypt depth ratio（P 〈 0.05） in the jejunum of pigs. Finally, benzoic acid supplementation increased the mR NA expression and concentration of glucagon-like peptide 2 and the activities of glutathione peroxidase and superoxide dismutase in the jejunal mucosa of young pigs（P 〈 0.05）.Conclusions： In conclusion, supplementation with 5000 mg/kg benzoic acid improved the performance of young pigs through promoting nutrient digestion, improving jejunal antioxidant capacity, and maintaining the jejunal morphology in young pigs.
基金the financial supports from the National Natural Science Foundation of China(Nos.52372037 and52072002)the Outstanding Scientific Research and Innovation Team Program of Higher Education Institutions of Anhui Province(No.2023AH010015)+1 种基金the Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province(No.2023AH030026)the financial support from the Anhui International Research Center of Energy Materials Green Manufacturing and Biotechnology.
文摘It is a big challenge to tune the structure and composition of carbon-based anode materials to increase the active sites by a green synthesis strategy for potassium ion batteries(PIBs).Herein,the N/F/S co-doped three-dimensional(3D)interconnected carbon nanosheets(NFS-CNSs)were synthesized from coal tar pitch(CTP)through a green and low-temperature treatment process for the first time.The as-obtained NFS-CNS600 features 3D interconnected ultra-thin carbon nanosheets with abundant active sites,tunable N/F/S species,and enlarged carbon interlayer spacing.The density functional theory calculation results demonstrate that NFS-CNSs exhibit the highest electron density and most negative K^(+)adsorption energy(-0.59 eV)compared to single or double-atom doping,thereby enhancing the storage performance of K+.As an anode for PIBs,the NFS-CNS600 exhibits good cycle stability(98.2%capacity retention after 200 cycles at 0.2 A g^(-1)),high capacity(409.1 mAh g^(-1) at 0.05 A g^(-1))and rate performance(179.5 mAh g^(-1) at 5 A g^(-1)).Besides,the NFS-CNS600 anode also displays outstanding sodium storage performance.This work offers a green strategy to synthesize CTP-based anode materials from coal chemical by-products for high-performance PIBs.
基金Sichuan Science and Technology Program(No.2021ZDZX0009).
文摘Background Oxidative stress significantly impacts growth performance and liver function in piglets.Ferulic acid(FA)works as an antioxidant,however,the role and mechanism of FA in the regulation of diquat-induced oxidative stress in piglets are less known.This study was designed to investigate the effects of FA on growth performance and antioxi-dant capacity in piglets with diquat challenge.Methods Thirty-two healthy DLY(Duroc×Landrace×Yorkshire)piglets(13.24±0.19 kg)were randomly divided into one of two diets including 0 or 4 g/kg FA for 14 d.On d 15,all pigs were intraperitoneally injected diquat or sterile saline.Results Dietary supplementation with ferulic acid(FA)significantly improved the average daily gain(ADG)and decreased feed-gain ratio(F/G)of piglets.Here,dietary FA supplementation reduced serum aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT)activities in diquat challenged piglets.Furthermore,diquat infusion increased reactive oxygen radicals(ROS)level in liver,decreased the activities of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and increased malondialdehyde(MDA)con-tent in the liver and serum.Supplementation with FA significantly increased T-AOC and T-SOD activities and decreased MDA and ROS levels.FA down-regulated gene and protein expression of Keap1,and up-regulated protein expression of Nrf2 and HO-1 in the liver of piglets with diquat challenge.Importantly,diquat challenge increased the ratio of late apoptosis,increased serum levels of IL-1β,IL-18 and lactate dehydrogenase(LDH),and up-regulated pyroptosis-related genes in the liver.FA supplementation reduced the ratio of late apoptosis and down-regulated mRNA expression of Caspase-1.Accordingly,FA addition reduced concentration of IL-1β,IL-18,and LDH under diquat challenge.Conclusions Diquat-induced oxidative stress reduced growth performance and impaired liver function in piglets.Dietary FA supplementation enhanced the antioxidant capacity and reduced the degree of hepatocyte pyroptosis,thereby alleviating the oxidative damage in the liver and mitigating the impact of diquat on growth performance of piglets.
基金Supported by National Natural Science Foundation of China,No.82300739Hunan Provincial Natural Science Foundation,No.2023JJ40821Changsha Natural Science Foundation,No.kq2208308.
文摘BACKGROUND Intestinal barrier dysfunction is a prevalent and varied manifestation of acute pancreatitis(AP).Molecular mechanisms underlying the early intestinal barrier in AP remain poorly understood.AIM To explore the biological processes and mechanisms of intestinal injury associated with AP,and to find potential targets for early prevention or treatment of intestinal barrier injury.METHODS This study utilized single-cell RNA sequencing of the small intestine,alongside in vitro and in vivo experiments,to examine intestinal barrier function homeostasis during the early stages of AP and explore involved biological processes and potential mechanisms.RESULTS Seventeen major cell types and 33232 cells were identified across all samples,including normal,AP1(4x caerulein injections,animals sacrificed 2 h after the last injection),and AP2(8x caerulein injections,animals sacrificed 4 h after the last injection).An average of 980 genes per cell was found in the normal intestine,compared to 927 in the AP1 intestine and 1382 in the AP2 intestine.B cells,dendritic cells,mast cells(MCs),and monocytes in AP1 and AP2 showed reduced numbers compared to the normal intestine.Enterocytes,brush cells,enteroendocrine cells,and goblet cells maintained numbers similar to the normal intestine,while cytotoxic T cells and natural killer(NK)cells increased.Enterocytes in early AP exhibited elevated programmed cell death and intestinal barrier dysfunction but retained absorption capabilities.Cytotoxic T cells and NK cells showed enhanced pathogen-fighting abilities.Activated MCs,secreted chemokine(C-C motif)ligand 5(CCL5),promoted neutrophil and macrophage infiltration and contributed to barrier dysfunction.CONCLUSION These findings enrich our understanding of biological processes and mechanisms in AP-associated intestinal injury,suggesting that CCL5 from MCs is a potential target for addressing dysfunction.
基金funded by the National Natural Science Foundation of China(22271063 and 22371054)the Foundation of Basic and Applied Basic Research of Guangdong Province(2024A1515010423)+1 种基金Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Z032)Science and Technology Planning Project of Guangdong Province(2021A0505030066).
文摘Oxygen evolution reaction(OER),a critical half-reaction in photocatalytic overall water splitting for producing hydrogen,is a key step toward sustainable energy conversion.Conventional photocatalysts often suffer from limited light absorption and rapid charge recombination,hindering their further applications.To address these challenges,we have designed and synthesized a novel series of self-sensitized metal-organic frameworks(MOFs),Fe_(2)MCDDB(M=Ni,Mn,or Co).By incorporating photosensitive ligands,we have achieved efficient charge separation and promoted the transfer of photogenerated electrons to the active metal sites for water oxidation.Among the series,Fe_(2)NiCDDB exhibits exceptional OER activity,achieving an oxygen evolution rate of 125.3μmol g^(−1)h^(−1)under visible light irradiation.Experimental and theoretical results reveal that the optimized electronic structure and prolonged excited-state lifetime of Fe_(2)NiCDDB contribute to its enhanced catalytic performance.This work provides a promising strategy for designing two-in-one MOF photocatalysts for water oxidation.
基金supported by financial support from the National Natural Science Foundation of China(Grant Nos.52309122 and U2340229)the Innovation Team of Changjiang River Scientific Research Institute(Grant No.CKSF2024329/YT).
文摘Large-scale and heavily jointed rocks have inherent planes of anisotropy and secondary structural planes,such as dominant joint sets and random fractures,which result in significant differences in their failure mechanism and deformation behavior compared to other rock types.To address this issue,inherent anisotropic rocks with large-scale and dense joints are considered to be composed of the rock matrix,inherent planes of anisotropy,and secondary structural planes.Then a new implicit continuum model called LayerDFN is developed based on the crack tensor and damage tensor theories to characterize the mechanical properties of inherent anisotropic rocks.Furthermore,the LayerDFN model is implemented in the FLAC3D software,and a series of numerical results for typical example problems is compared with those obtained from the 3DEC,the analytical solutions,similar classical models,laboratory uniaxial compression tests,and field rigid bearing plate tests.The results demonstrate that the LayerDFN model can effectively capture the anisotropic mechanical properties of inherent anisotropic rocks,and can quantitatively characterize the damaging effect of the secondary structural planes.Overall,the numerical method based on the LayerDFN model provides a comprehensive and reliable approach for describing and analyzing the behavior of inherent anisotropic rocks,which will provide valuable insights for engineering design and decision-making processes.
基金supported by Funding was provided by grants from the Changsha Chinese Medicine Foundation(Grant No.B202314)the Natural Science Foundation of Hunan Province,China(Grant No.2024JJ8224)+1 种基金Changsha Municipal Natural Science Foundation(Grant No.kq2403187)Hunan Province Children’s Safe Medication Clinical Medical Technology Demonstration Base(Grant No.2023SK4083).
文摘Pediatric congenital heart disease(CHD)pharmacotherapy faces three fundamental barriers:developmental pharmacokinetic complexity,anatomic-genetic heterogeneity,and evidence chain gaps.Traditional agents exhibit critical limitations:digoxin’s narrow therapeutic index(0.5–0.9 ng/mL)is exacerbated by ABCB1 mutations(toxicity risk increases 4.1-fold),furosemide efficacy declines by 35%in neonates due to NKCC2 immaturity,andβ-blocker responses vary by CYP2D6 polymorphisms(poor metabolizers require 50–75%dose reduction).Novel strategies demonstrate transformative potential—CRISPR editing achieves 81%reversal of BMPR2-associated pulmonary vascular remodeling,metabolically matured cardiac organoids replicate adult myocardial energy metabolism for drug screening,and SGLT2 inhibitors activate triple mechanisms(calcium overload mitigation,mitophagy,fibrosis reversal).However,clinical translation requires overcoming developmental barriers:age-dependent enzyme expression(infant CYP2D6=30–60%adult activity),post-Fontan hepatotoxicity(bosentan trough concentrations elevates 1.8-fold),and AI model limitations(32%error in complex CHD).Future integration of placental transfer models,disease-specific organoids,and multi-omics mapping of FOXO/CRIM1 pathways will shift paradigms from symptom control to curative repair.
文摘To address the issues of unclear carbon responsibility attribution,insufficient renewable energy absorption,and simplistic carbon trading mechanisms in integrated energy systems,this paper proposes an electricheat-hydrogen integrated energy system(EHH-IES)optimal scheduling model considering carbon emission stream(CES)and wind-solar accommodation.First,the CES theory is introduced to quantify the carbon emission intensity of each energy conversion device and transmission branch by defining carbon emission rate,branch carbon intensity,and node carbon potential,realizing accurate tracking of carbon flow in the process of multi-energy coupling.Second,a stepped carbon pricing mechanism is established to dynamically adjust carbon trading costs based on the deviation between actual carbon emissions and initial quotas,strengthening the emission reduction incentive.Finally,a lowcarbon economic dispatch model is constructed with the objectives of minimizing operation cost,carbon trading cost,wind-solar curtailment penalty cost,and energy loss.Simulation results show that compared with the traditional economic dispatch scheme 3,the proposed schemel reduces carbon emissions by 53.97%and wind-solar curtailment by 68.89%with a 16.10%increase in total cost.This verifies that the model can effectively improve clean energy utilization and reduce carbon emissions,achieving low-carbon economic operation of EHH-IES,with CES theory ensuring precise carbon flow tracking across multi-energy links.
基金supported by Natural Science Foundation of Sichuan Province(2023NSFSC0237)National Natural Science Foundation of China(32372900)Major Special Projects in Sichuan Province(2021ZDZX0009).
文摘Background Inflammatory bowel disease is a significant health concern for both humans and large-scale farm animals.In the quest for effective alternatives to antibiotics,next-generation probiotics(NGPs)have emerged as a promising option.The genus Blautia presents a rich source of potential NGP strains.Here we successfully isolated Blautia hominis LYH1 strain from the intestines of healthy weaned piglets and characterized its biological traits.Its antiinflammatory activity was then assessed using macrophages,while its protective effects against colitis and gut barrier damage were validated in a DSS-induced mouse colitis model.Results B.hominis LYH1 displayed typical characteristics of an obligate anaerobe,including non-hemolytic and nonmotile features,and a genome enriched with carbohydrate-active enzyme genes.It produced metabolites with antibiotic-like compounds,demonstrating antimicrobial activity against Escherichia coli.In vitro,B.hominis LYH1 effectively inhibited pathogen replication in macrophages,reducing cellular infections and alleviating inflammatory damage.In vivo,oral administration of B.hominis LYH1 or its metabolites significantly mitigated DSS-induced colitis in mice by suppressing pro-inflammatory cytokines,inhibiting T-lymphocyte activation,and enhancing short-chain fatty acid production.Conclusions Our findings underscore B.hominis LYH1’s potential as a NGP for maintaining gut health and combating intestinal inflammation.These findings offer valuable insights into the development of antibiotic alternatives and innovative strategies for preventing and treating enteritis in both agricultural and medical settings.
基金the National Key Basic Research Program of China(No.2015CB5540007)the National Natural Science Foundation of China(No.81572413,No.81702386)+1 种基金the Fundamental Research Funds for the Central Universities(No.2017KFYXJJ256)Scientific and Technological Application Foundation Project of Wuhan(No.2015060101010044).
文摘Hepatoid adenocarcinoma of the stomach (HAS)is an extremely rare and unique gastric malignancy.The present study aimed to examine the relevance of the clinicopathological characteristics of HAS with patient prognosis.We retrospectively reviewed clinical data of 34 HAS patients treated at our institution between January 2010 and December 2016,as well as 294 cases reported prior to 2017 in research databases.Among these patients,45.6%(115/252)had lesions in the gastric antrum and 77.0%(235/305)were male.Elevated levels of serum alpha-fetoprotein (AFP)were detected in most patients(75/93,80.6%).Vascular invasion(199/286,69.6%),lymph node metastasis (222/283,78.4%),and preoperative distant metastasis (121/328,36.9%)were commonly observed.The 5-year disease-free survival (DFS)and disease-specific survival (DSS) were 20.7%and 29.2%,respectively.DFS and DSS of patients receiving neoadjuvant therapy were significantly higher than those of patients receiving postoperative adjuvant therapy [DFS:P<0.001, hazard ratio (HR)=-1.831,95%confidence interval (CI):0.060-0.429;DSS:P<0.001,HR=-2.185, 95%CI:0.032-0.401].In conclusion,HAS exhibits distinct clinicopathological characteristics and a strikingly worse prognosis when compared with common gastric cancer.Complete surgery,early pTNM stage,and adjuvant therapy may predict a more favorable prognosis.Neoadjuvant therapy is strongly recommended for patients with lymph node metastasis or/and preoperative distant metastasis.
基金This study was financially supported by the grant from the earmarked fund for the China Agriculture Research System(CARS-35)the fund from the Project of SAU-Adisseo Centre of Research on Nutrition and Healththe fund from Agricultural Science and Technology Achievement Transformation Fund Project of Sichuan Province(2016NYZ0052 and 20NZZH0057).
文摘Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest(201403047)
文摘Background: Alginate oligosaccharide(AOS), produced from alginate by alginate lyase-mediated depolymerisation, is a potential substitute for antibiotics and possesses growth-enhancing effects. Nevertheless, the mechanisms by which AOS regulates porcine growth remain to be elucidated. Therefore, we investigated the AOS-mediated changes in the growth performance of weaned pigs by determining the intestinal morphology, barrier function,as well as epithelium apoptosis.Methods: Twenty-four weaned pigs were distributed into two groups(n = 12) and received either a basal diet(control group) or the same diet supplemented with 100 mg/kg AOS. On d 15, D-xylose(0.1 g/kg body weight)was orally administrated to eight randomly selected pigs per treatment, and their serum and intestinal mucosa samples were collected 1 h later.Results: Our results showed that inclusion of AOS in the diet for 2 wk increased(P < 0.05) the average daily body weight gain in weaned pigs. Notably, AOS supplementation ameliorated the intestinal morphology and barrier function, as suggested by the enhanced(P < 0.05) intestinal villus height, secretory immunoglobulin A content and goblet cell counts. Compared to the control group, AOS ingestion both decreased(P < 0.05) the total apoptotic percentage and increased(P < 0.05) the proportion of S phase in the intestinal epithelial cells. Furthermore, AOS not only up-regulated(P < 0.05) the B-cell lymphoma-2(BCL2) transcriptional level but also down-regulated(P < 0.05) the B-cell lymphoma-2-associated X protein(BAX), cysteinyl aspartate-specific proteinase-3(caspase-3) and caspase-9 transcriptional levels in the small intestine.Conclusions: In summary, this study provides evidence that supplemental AOS beneficially affects the growth performance of weaned pigs, which may result from the improved intestinal morphology and barrier function,as well as the inhibited enterocyte death, through reducing apoptosis via mitochondria-dependent apoptosis.
基金financially supported by the earmarked fund for the China Agriculture Research System(CARS-36)the grant from the Science and Technology Support Program of Sichuan Province(13ZC2237)
文摘Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0A5% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCI solution or diquat （10 mg/kg body weight）. During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain （P = 0.09） and average daily feed intake （P = 0.08）, and decreased the feed efficiency （P 〈 0.05） of piglets. The growth performance was decreased by diquat injection （P 〈 0.05）. Diquat injection also decreased the activities of the superoxide dismutase （SOD） and glutathione peroxidase （GPx） in the plasma and liver （P 〈 0.05）, increased plasma malondialdehyde （MDA） （P 〈 0.05） and urea nitrogen （P 〈 0.05） concentrations, and enhanced MDA concentration （P = 0.09） and tryptophan 2,3-dioxygenase （TDO） activity （P = 0.07） in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA （P = 0.06） concentration and the activities of SOD （P = 0.09） and GPx （P = 0.05） of the liver, and plasma urea nitrogen （P = 0.06） concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection （P 〈 0.05）. These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.
基金funded by the Natural Science Foundations of China(No.32172702)National Key Research and Development Program of China(2021YFD1301101)Agricultural Science and Technology Innovation Program(ASTIP-IAS02)。
文摘Background Genomic selection involves choosing as parents those elite individuals with the higher genomic estimated breeding values(GEBV)to accelerate the speed of genetic improvement in domestic animals.But after multi-generation selection,the rate of inbreeding and the occurrence of homozygous harmful alleles might increase,which would reduce performance and genetic diversity.To mitigate the above problems,we can utilize genomic mating(GM)based upon optimal mate allocation to construct the best genotypic combinations in the next generation.In this study,we used stochastic simulation to investigate the impact of various factors on the efficiencies of GM to optimize pairing combinations after genomic selection of candidates in a pig population.These factors included:the algorithm used to derive inbreeding coefficients;the trait heritability(0.1,0.3 or 0.5);the kind of GM scheme(focused average GEBV or inbreeding);the approach for computing the genomic relationship matrix(by SNP or runs of homozygosity(ROH)).The outcomes were compared to three traditional mating schemes(random,positive assortative or negative assortative matings).In addition,the performance of the GM approach was tested on real datasets obtained from a Large White pig breeding population.Results Genomic mating outperforms other approaches in limiting the inbreeding accumulation for the same expected genetic gain.The use of ROH-based genealogical relatedness in GM achieved faster genetic gains than using relatedness based on individual SNPs.The GROH-based GM schemes with the maximum genetic gain resulted in 0.9%-2.6%higher rates of genetic gainΔG,and 13%-83.3%lowerΔF than positive assortative mating regardless of heritability.The rates of inbreeding were always the fastest with positive assortative mating.Results from a purebred Large White pig population,confirmed that GM with ROH-based GRM was more efficient than traditional mating schemes.Conclusion Compared with traditional mating schemes,genomic mating can not only achieve sustainable genetic progress but also effectively control the rates of inbreeding accumulation in the population.Our findings demonstrated that breeders should consider using genomic mating for genetic improvement of pigs.
基金supported by NSF of China(Grant No.61775241)partly by the Innovation-driven Project(Grant No.2017CX019)the funding support from the Australian Research Council(ARC Discovery Projects,DP180102976)
文摘With a large number of researches being conducted on two?dimen?sional(2D)materials,their unique properties in optics,electrics,mechanics,and magnetics have attracted increasing attention.Accordingly,the idea of combining distinct functional 2D materials into heterostructures naturally emerged that pro?vides unprecedented platforms for exploring new physics that are not accessible in a single 2D material or 3D heterostructures.Along with the rapid development of controllable,scalable,and programmed synthesis techniques of high?quality 2D heterostructures,various heterostructure devices with extraordinary performance have been designed and fabricated,including tunneling transistors,photodetectors,and spintronic devices.In this review,we present a summary of the latest progresses in fabrications,properties,and applications of di erent types of 2D heterostruc?tures,followed by the discussions on present challenges and perspectives of further investigations.
基金supported by the National Natural Science Foundation of China (31972599)the Key Research and Development Program of Sichuan Province (2020YFN0147)
文摘Background:Sialyllactose(SL)is one of the most abundant oligosaccharides present in porcine breast milk.However,little is known about its effect on growth performance and intestinal health in weaned pigs.This study was conducted to explore the protective effect of SL on intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli(ETEC)challenge.Methods:Thirty-two pigs were randomly divided into four treatments.Pigs fed with a basal diet or basal diet containing SL(5.0 g/kg)were orally infused with ETEC or culture medium.Results:SL supplementation elevated the average daily gain(ADG)and feed efficiency in the ETEC-challenged pigs(P<0.05).SL also improved the digestibilities of dry matter(DM),gross energy(GE),and ash in non-challenged pigs(P<0.05).Moreover,SL not only elevated serum concentrations of immunoglobulins(IgA,IgG,and IgM),but also significantly decreased the serum concentrations of inflammatory cytokines(TNF-α,IL-1β,and IL-6)upon ETEC challenge(P<0.05).Interestingly,SL increased the villus height,the ratio of villus height to crypt depth(V:C),and the activities of mucosal sucrase and maltase in the jejunum and ileum(P<0.05).SL also elevated the concentrations of microbial metabolites(e.g.acetic acid,propanoic acid,and butyric acid)and the abundance of Lactobacillus,Bifidobacterium,and Bacillus in the cecum(P<0.05).Importantly,SL significantly elevated the expression levels of jejunal zonula occludins-1(ZO-1),occluding,and fatty acid transport protein-4(FATP4)in the ETEC-challenged pigs(P<0.05).Conclusions:SL can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge,which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins,as well as improved intestinal epithelium functions and microbiota.
