Objective In this study,we analyzed the transcriptome sequences of Kupffer cells exposed to simulated microgravity for 3 d and conducted biological experiments to determine how microgravity initiates apoptosis in Kupf...Objective In this study,we analyzed the transcriptome sequences of Kupffer cells exposed to simulated microgravity for 3 d and conducted biological experiments to determine how microgravity initiates apoptosis in Kupffer cells.Methods Rotary cell culture system was used to construct a simulated microgravity model.GO and KEGG analyses were conducted using the DAVID database.GSEA was performed using the R language.The STRING database was used to conduct PPI analysis.qPCR was used to measure the IL1B,TNFA,CASP3,CASP9,and BCL2L11 mRNA expressions.Western Blotting was performed to detect the level of proteins CASP3 and CASP 9.Flow cytometry was used to detect apoptosis and mitochondrial membrane cells.Transmission electron microscopy was used to detect changes in the ultrastructure of Kupffer cells.Results Transcriptome Sequencing indicated that simulated microgravity affected apoptosis and the inflammatory state of Kupffer cells.Simulated microgravity improved the CASP3,CASP9,and BCL2L11 expressions in Kupffer cells.Annexin-V/PI and JC-1 assays showed that simulated microgravity promoted apoptosis in Kupffer cells.Simulated microgravity causes M1 polarization in Kupffer cells.Conclusion Our study found that simulated microgravity facilitated the apoptosis of Kupffer cells through the mitochondrial pathway and activated Kupffer cells into M1 polarization,which can secrete TNFA to promote apoptosis.展开更多
5-Hydroxymethylfurfural(HMF)and its oxidation derivatives have emerged as a bridge between biomass resources and the future energy industry.These renewable biomass resources can be transformed into a variety of value-...5-Hydroxymethylfurfural(HMF)and its oxidation derivatives have emerged as a bridge between biomass resources and the future energy industry.These renewable biomass resources can be transformed into a variety of value-added chemicals,thereby addressing the challenges posed by diminishing fossil fuel reserves and environmental concerns.The immobilization of catalysts represents an innovative method for the sustainable and efficient synthesis of HMF and its oxidation derivatives.This method not only enhances the yield and selectivity of the products but also allows for the optimization of the catalytic performance of immobilized catalysts through the strategic design of their supports.In this review,we provide an overview of the recent advancements in the technology of immobilized catalyst and its application in the synthesis of HMF and its oxidation derivatives,with a particular focus on the preparation and catalytic characteristics of these immobilized catalysts.Furthermore,we discuss potential future directions for the development of immobilized catalysts,including the preparation of high-performance immobilized catalysts,the exploration of their growth and catalytic mechanisms,and the economic implications of raw material utilization.This area of research presents both significant promise and considerable challenges.展开更多
Transition metal dichalcogenides, featuring layered structures, have aroused enormous interest as a platform for novel physical phenomena and a wide range of potential applications. Among them, special interest has be...Transition metal dichalcogenides, featuring layered structures, have aroused enormous interest as a platform for novel physical phenomena and a wide range of potential applications. Among them, special interest has been placed upon WTe_2 and MoTe_2, which exhibit non-trivial topology both in single layer and bulk as well as pressure induced or enhanced superconductivity. We study another distorted IT material NbTe_2 through systematic electrical transport measurements. Intrinsic superconductivity with onset transition temperature(T_c^(onset)) up to 0.72 K is detected where the upper critical field(H_c) shows unconventional quasi-linear behavior,indicating spin-orbit coupling induced p-wave paring. Furthermore, a general model is proposed to fit the angledependent magnetoresistance, which reveals the Fermi surface anisotropy of NbTe_2. Finally, non-saturating linear magnetoresistance up to 50 T is observed and attributed to the quantum limit transport.展开更多
Despite the long availability of a traditional prophylactic vaccine containing the HBV surface antigen(HBsA g) and aluminum adjuvant, nearly 10% of the population remains unable to generate an effective immune respons...Despite the long availability of a traditional prophylactic vaccine containing the HBV surface antigen(HBsA g) and aluminum adjuvant, nearly 10% of the population remains unable to generate an effective immune response. Previous studies have indicated that hepatitis B virus(HBV) PreS 2-S is abundant in T/B cell epitopes, which induces a stronger immune response than HBsA g, particularly in terms of cytotoxic T lymphocyte(CTL) reaction. In the current study, the HBV PreS 2-S gene encoding an extra26 amino acids(PreS 2 C-terminus) located at the N-terminus of HBsA g was cloned into the pV CH1300 expression vector. Pre S2-S expressed in the methylotrophic yeast, Hansenula polymorpha, was produced at a yield of up to 250 mg/L. Subsequent purification steps involved hydrophobic adsorption to colloidal silica, ion-exchange chromatography and density ultracentrifugation. The final product was obtained with a total yield of ~15% and purity of ~99%. In keeping with previous studies, ~22 nm viruslike particles were detected using electron microscopy. The generated PreS 2-S antigen will be further studied for efficacy and safty in animals.展开更多
Increasing the urban tree cover percentage(TCP) is widely recognized as an efficient way to mitigate the urban heat island effect. The cooling efficiency of urban trees can be either enhanced or attenuated on hotter d...Increasing the urban tree cover percentage(TCP) is widely recognized as an efficient way to mitigate the urban heat island effect. The cooling efficiency of urban trees can be either enhanced or attenuated on hotter days, depending on the physiological response of urban trees to rising ambient temperature. However, the response of urban trees' cooling efficiency to rising urban temperature remains poorly quantified for China's cities. In this study, we quantify the response of urban trees' cooling efficiency to rising urban temperature at noontime [~1330 LT(local time), LT=UTC+8] in 17summers(June, July, and August) from 2003–19 in 70 economically developed cities of China based on satellite observations. The results show that urban trees have stronger cooling efficiency with increasing temperature, suggesting additional cooling benefits provided by urban trees on hotter days. The enhanced cooling efficiency values of urban trees range from 0.002 to 0.055℃ %-1 per 1℃ increase in temperature across the selected cities, with larger values for the lowTCP-level cities. The response is also regulated by background temperature and precipitation, as the additional cooling benefit tends to be larger in warmer and wetter cities at the same TCP level. The positive response of urban trees' cooling efficiency to rising urban temperature is explained mainly by the stronger evapotranspiration of urban trees on hotter days.These results have important implications for alleviating urban heat risk by utilizing urban trees, particularly considering that extreme hot days are becoming more frequent in cities under global warming.展开更多
The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis....The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis.Expanding enzyme catalysis to a larger substrate scope and greater variety of chemical reactions and tuning the microenvironment surrounding enzyme molecules to achieve high enzyme performance are urgently needed.In this account,we focus on our efforts using the de novo approach to synthesis hybrid enzyme catalysts that can address these two challenges and the structure-function relationship is discussed to reveal the principles of designing hybrid enzyme catalysts.We hope that this account will promote further efforts toward fundamental research and wide applications of designed enzyme hybrid catalysts for expanding biocatalysis.展开更多
Flexible and environment-responsive materials are essential for a large number of applications from artificial skin to wearable devices. The present study develops a flexible, ultra-low cost conductive hybrid elastome...Flexible and environment-responsive materials are essential for a large number of applications from artificial skin to wearable devices. The present study develops a flexible, ultra-low cost conductive hybrid elastomer(CHE), which possesses high responsive capabilities to stress/strain and humidity. CHE was composed of polydimethylsiloxane(PDMS) and starch hydrogel(SH), enabling great elasticity(56 kPa),high conductivity(10^(-2)S/m) and high sensitivity to external stimuli(gauge factor of CHE under stress and strain are 0.71 and 2.22, respectively, and sensitivity to humidity is 1.2 × 10^(-6)S/m per RH%). These properties render CHE a promising candidate for artificial skin and wearable electronics applications of continuously monitoring environmental information.展开更多
Online traffic simulation that feeds from online information to simulate vehicle movement in real-time has recently seen substantial advancement in the development of intelligent transportation systems and urban traff...Online traffic simulation that feeds from online information to simulate vehicle movement in real-time has recently seen substantial advancement in the development of intelligent transportation systems and urban traffic management.It has been a challenging problem due to three aspects:1)The diversity of traffic patterns due to heterogeneous layouts of urban intersections;2)The nature of complex spatiotemporal correlations;3)The requirement of dynamically adjusting the parameters of traffic models in a real-time system.To cater to these challenges,this paper proposes an online traffic simulation framework called automated urban traffic operation simulation via meta-learning(AUTOSIM).In particular,simulation models with various intersection layouts are automatically generated using an open-source simulation tool based on static traffic geometry attributes.Through a meta-learning technique,AUTOSIM enables an automated learning process for dynamic model settings of traffic scenarios featured with different spatiotemporal correlations.Besides,AUTOSIM is capable of adapting traffic model parameters according to dynamic traffic information in real-time by using a meta-learner.Through computational experiments,we demonstrate the effectiveness of the meta-learningbased framework that is capable of providing reliable supports to real-time traffic simulation and dynamic traffic operations.展开更多
Palladium(Pd)‐based catalysts are essential to drive high‐performance Suzuki coupling reactions,which are powerful tools for the synthesis of functional organic compounds.Herein,we developed a solution‐rapid‐annea...Palladium(Pd)‐based catalysts are essential to drive high‐performance Suzuki coupling reactions,which are powerful tools for the synthesis of functional organic compounds.Herein,we developed a solution‐rapid‐annealing process to stabilize nitrogen‐mesoporous carbon supported Pd single‐atom/cluster(Pd/NMC)material,which provided a catalyst with superior performance for Suzuki coupling reactions.In comparison with commercial palladium/carbon(Pd/C)catalysts,the Pd/NMC catalyst exhibited significantly boosted activity(100%selectivity and 95%yield)and excellent stability(almost no decay in activity after 10 reuse cycles)for the Suzuki coupling reactions of chlorobenzenes,together with superior yield and excellent selectivity in the fields of the board scope of the reactants.Moreover,our newly developed rapid annealing process of precursor solutions is applied as a generalized method to stabilize metal clusters(e.g.Pd,Pt,Ru),opening new possibilities in the construction of efficient highly dispersed metal atom and sub‐nanometer cluster catalysts with high performance.展开更多
From theories and cases,this paper analyzed current situation of subsidy policy system for agricultural natural disasters in China.Improvement and perfection of subsidy policies for agricultural natural disasters need...From theories and cases,this paper analyzed current situation of subsidy policy system for agricultural natural disasters in China.Improvement and perfection of subsidy policies for agricultural natural disasters need clear classification and additional special subsidy plan.It is recommended to improve and perfect subsidy policy system for agricultural natural disasters through attaching importance to scientific studies on reduction and prevention of agricultural disasters and gradually supplementing agricultural disaster reduction and prevention system.展开更多
BACKGROUND Vascular injury is a rare complication of femoral shaft fractures,and rupture of the deep femoral artery is more difficult to diagnose because of its anatomical location and symptoms.Despite its low inciden...BACKGROUND Vascular injury is a rare complication of femoral shaft fractures,and rupture of the deep femoral artery is more difficult to diagnose because of its anatomical location and symptoms.Despite its low incidence,deep femoral artery rupture can lead to life-threatening outcomes,such as compartment syndrome,making early identification and diagnosis critical.CASE SUMMARY A 45-year-old male patient was admitted to our hospital due to right lower limb trauma in a car accident,with complaints of severe pain and swelling on his right thigh.X-ray demonstrated a right femoral shaft fracture.During preparation for emergency surgery,his blood pressure and blood oxygen saturation dropped,and sensorimotor function was lost.Computed tomography angiography was performed immediately to confirm the diagnosis of rupture of the deep femoral artery and compartment syndrome,so fasciotomy and vacuum-assisted closure were performed.Rhabdomyolysis took place after the operation and the patient was treated with appropriate electrolyte correction and diuretic therapy.Twenty days after the fasciotomy,treatment with the Hoffman Type II External Fixation System was planned,but it was unable to be immobilized internally based on a new esophageal cancer diagnosis.We kept the external fixation for 1 year,and 3 years of follow-up showed improvement of the patient’s overall conditions and muscle strength.CONCLUSION For patients with thigh swelling,pain,anemia,and unstable vital signs,anterior femoral artery injury should be highly suspected.Once diagnosed,surgical treatment should be performed immediately and complications of artery rupture must be suspected and addressed in time.展开更多
Rice is a staple food for half of the world’s population,but it is a poor dietary source of calcium(Ca)due to the low concentration.It is an important issue to boost Ca concentration in this grain to improve Ca defic...Rice is a staple food for half of the world’s population,but it is a poor dietary source of calcium(Ca)due to the low concentration.It is an important issue to boost Ca concentration in this grain to improve Ca deficiency risk,but the mechanisms underlying Ca accumulation are poorly understood.Here,we obtained a rice(Oryza sativa)mutant with high shoot Ca accumulation.The mutant exhibited 26%-53% higher Ca in shoots than did wild-type rice(WT)at different Ca supplies.Ca concentration in the xylem sap was 36% higher in the mutant than in the WT.There was no difference in agronomic traits between the WT and mutant,but the mutant showed 25% higher Ca in the polished grain compared with the WT.Map-based cloning combined with a complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene,OsK5.2,belonging to the Shaker-like K+channel family.OsK5.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels,but upregulated by low K.Immunostaining showed that OsK5.2 was mainly expressed in the pericycle of the roots.Taken together,our results revealed a novel role for OsK5.2 in Ca translocation in rice,and will be a good target for Ca biofortification in rice.展开更多
Implant-related osteomyelitis is a formidable hurdle in the clinical setting and is characterized by inflammation,infection,and consequential bone destruction.Therefore,effective reactive oxygen species(ROS)scavenging...Implant-related osteomyelitis is a formidable hurdle in the clinical setting and is characterized by inflammation,infection,and consequential bone destruction.Therefore,effective reactive oxygen species(ROS)scavenging,bacterial killing,and subsequent bone tissue repair are urgently needed for the treatment of difficult-to-heal osteomyelitis.Herein,we utilized the eddy-thermal effect of magnesium(Mg)implants under an alternating magnetic field(AMF)for the controlled release of H_(2)gas and ions(OH^(-)and Mg^(2+))for the treatment of osteomyelitis.H_(2)released by Mg rods under AMFs effectively scavenged cytotoxic ROS,exhibiting anti-inflammatory effects and consequently disrupting the environment of bacterial infections.In addition,the OH-hindered the energy metabolism of bacteria by effectively neutralizing protons within the microenvironment.Moreover,H_(2)impaired the permeability of bacterial membranes and expedited the damage induced by OH-.This synergistic AMF-induced H_(2)and proton depletion treatment approach not only killed both gram-negative and gram-positive bacteria but also effectively treated bacterial infections(abscesses and osteomyelitis).Moreover,Mg^(2+)released from the Mg rods enhanced and accelerated the process of bone osteogenesis.Overall,our work cleverly exploited the eddy-thermal effect and chemical activity of Mg implants under AMFs,aiming to eliminate the inflammatory environment and combat bacterial infections by the simultaneous release of H_(2),OH^(-),and Mg^(2+),thereby facilitating tissue regeneration.This therapeutic strategy achieved multiple benefits in one,thus presenting a promising avenue for clinical application.展开更多
Catalysis plays an important role in human civilization and economic development.Every breakthrough related to the field of catalysis has great impact on our everyday lives.To achieve harmonious development between hu...Catalysis plays an important role in human civilization and economic development.Every breakthrough related to the field of catalysis has great impact on our everyday lives.To achieve harmonious development between human and nature through developing chemical processes with environmental friendliness and atom economy,the improvement and innovation of catalytic systems have drawn great attention.Biocatalysis is the engine of life from the very beginning,which is the example that we always learn from,mimic,and aim to surpass in scientific research and industrial development.The goal of the development of biocatalysis technology is tomake our industrial manufacturing go undermild conditions as nature does.However,the current situation of biocatalysis application is still far from the ultimate aim,providing countless opportunities for further development.展开更多
Waterborne microbial contamination represents a significant global environmental challenge.Microorganisms can induce water pollution,precipitating infectious disease outbreaks and posing a substantial threat to human ...Waterborne microbial contamination represents a significant global environmental challenge.Microorganisms can induce water pollution,precipitating infectious disease outbreaks and posing a substantial threat to human health.Currently,the excessive use of conventional antibiotics has resulted in the emergence of bacterial resistance,posing a challenge to the design and development of antimicrobial agents with high antibacterial efficacy in diverse water such as rivers,lakes,and wastewater from microbiology laboratories.Bimetallic nanomaterials,that amalgamate the distinctive characteristics of diverse metals,are garnering attention due to their synergistic properties.In this study,PVP-FeMo_(2)S_(3)(PVP:Polyvinylpyrrolidone)and PVP-ZnMo_(2)S_(3)nanocrystals were synthesized by a high-temperature reaction.The prepared nanocrystals exhibited excellent biocompatibility,regular morphology,long-term stability,and remarkable antibacterial e ects.The bacterial biofilms could be eradicated by the combination of photothermal(PTT)and photodynamic synergistic e ects of PVP-FeMo_(2)S_(3)and PVP-ZnMo_(2)S_(3)nanocrystals.PVP-ZnMo_(2)S_(3)nanocrystals exhibited enhanced PTT conversion efficiency for water treatment.Remarkably,even after 20 cycles of recycling,PVP-ZnMo_(2)S_(3)retained substantial antibacterial e cacy.The excellent biosafety of PVP-ZnMo_(2)S_(3)further guarantees the safety of water quality post-treatment.Thus,PVP-ZnMo_(2)S_(3)bimetallic nanocrystals can serve as a novel antimicrobial agent for the treatment of microbial contamination in water.This study offers a potential strategy to mitigate secondary water pollution and prevent the development of drug resistance among pathogenic microorganisms.展开更多
Since the fault dynamic of droop-controlled inverter is different from synchronous generators (SGs), protection devices may become invalid, and the fault overcurrent may damage power electronic devices and threaten th...Since the fault dynamic of droop-controlled inverter is different from synchronous generators (SGs), protection devices may become invalid, and the fault overcurrent may damage power electronic devices and threaten the safety of the microgrid. Therefore, it is imperative to conduct a comprehensive fault analysis of the inverter to guide the design of protection schemes. However, due to the complexity of droop control strategy, existing literatures have simplified asymmetric fault analysis of droop-controlled inverters to varying degrees. Therefore, accurate fault analysis of a droop-controlled inverter is needed. In this paper, by analyzing the control system, an accurate fault model is established. Based on this, a calculation method for instantaneous asymmetrical fault current is proposed. In addition, the current components and current characteristics are analyzed. It was determined that fault currents are affected by control loops, fault types, fault distance and nonlinear limiters. In particular, the influences of limiters on the fault model, fault current calculation and fault current characteristics were analyzed. Through detailed analysis, it was found that dynamics of the control loop cannot be ignored, the fault type and fault distance determine fault current level, and part of the limiters will totally change the fault current trend. Finally, calculation and experimental results verify the correctness of the proposed method.展开更多
Objective:To evaluate the therapeutic effect of dynamic fixation with a hook plate-double loop plate internal fixation system for high-energy acromioclavicular joint complex dislocation(denoted as HACD,i.e.,Rockwood t...Objective:To evaluate the therapeutic effect of dynamic fixation with a hook plate-double loop plate internal fixation system for high-energy acromioclavicular joint complex dislocation(denoted as HACD,i.e.,Rockwood type III-V dislocation).Methods:Fifty-eight patients with HACD were selected and evenly divided by drawing lots.The experimental group underwent ligament reconstruction treatment,while the reference group received hook plate fixation treatment.The efficacy and other indicators were compared between the groups.Results:The overall effective rates between the groups were similar(P>0.05).The experimental group had a longer surgical duration,lower pain scores at 6 months postoperatively,higher shoulder joint function scores,and a lower complication rate,with P<0.05 when compared between groups.Conclusion:The effectiveness of the double loop plate internal fixation system ligament reconstruction treatment for patients with HACD is comparable to that of hook plate fixation treatment.Although the surgical duration is slightly longer,postoperative pain is milder,facilitating the recovery of shoulder joint function in patients and offering higher safety.展开更多
文摘Objective In this study,we analyzed the transcriptome sequences of Kupffer cells exposed to simulated microgravity for 3 d and conducted biological experiments to determine how microgravity initiates apoptosis in Kupffer cells.Methods Rotary cell culture system was used to construct a simulated microgravity model.GO and KEGG analyses were conducted using the DAVID database.GSEA was performed using the R language.The STRING database was used to conduct PPI analysis.qPCR was used to measure the IL1B,TNFA,CASP3,CASP9,and BCL2L11 mRNA expressions.Western Blotting was performed to detect the level of proteins CASP3 and CASP 9.Flow cytometry was used to detect apoptosis and mitochondrial membrane cells.Transmission electron microscopy was used to detect changes in the ultrastructure of Kupffer cells.Results Transcriptome Sequencing indicated that simulated microgravity affected apoptosis and the inflammatory state of Kupffer cells.Simulated microgravity improved the CASP3,CASP9,and BCL2L11 expressions in Kupffer cells.Annexin-V/PI and JC-1 assays showed that simulated microgravity promoted apoptosis in Kupffer cells.Simulated microgravity causes M1 polarization in Kupffer cells.Conclusion Our study found that simulated microgravity facilitated the apoptosis of Kupffer cells through the mitochondrial pathway and activated Kupffer cells into M1 polarization,which can secrete TNFA to promote apoptosis.
文摘5-Hydroxymethylfurfural(HMF)and its oxidation derivatives have emerged as a bridge between biomass resources and the future energy industry.These renewable biomass resources can be transformed into a variety of value-added chemicals,thereby addressing the challenges posed by diminishing fossil fuel reserves and environmental concerns.The immobilization of catalysts represents an innovative method for the sustainable and efficient synthesis of HMF and its oxidation derivatives.This method not only enhances the yield and selectivity of the products but also allows for the optimization of the catalytic performance of immobilized catalysts through the strategic design of their supports.In this review,we provide an overview of the recent advancements in the technology of immobilized catalyst and its application in the synthesis of HMF and its oxidation derivatives,with a particular focus on the preparation and catalytic characteristics of these immobilized catalysts.Furthermore,we discuss potential future directions for the development of immobilized catalysts,including the preparation of high-performance immobilized catalysts,the exploration of their growth and catalytic mechanisms,and the economic implications of raw material utilization.This area of research presents both significant promise and considerable challenges.
基金Supported by the National Basic Research Program of China under Grant Nos 2018YFA0305600 and 2017YFA0303302the National Natural Science Foundation of China under Grant Nos 11888101,11774008,11704414 and 11427805+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences under Grant No XDB28000000Beijing Natural Science Foundation(Z180010)
文摘Transition metal dichalcogenides, featuring layered structures, have aroused enormous interest as a platform for novel physical phenomena and a wide range of potential applications. Among them, special interest has been placed upon WTe_2 and MoTe_2, which exhibit non-trivial topology both in single layer and bulk as well as pressure induced or enhanced superconductivity. We study another distorted IT material NbTe_2 through systematic electrical transport measurements. Intrinsic superconductivity with onset transition temperature(T_c^(onset)) up to 0.72 K is detected where the upper critical field(H_c) shows unconventional quasi-linear behavior,indicating spin-orbit coupling induced p-wave paring. Furthermore, a general model is proposed to fit the angledependent magnetoresistance, which reveals the Fermi surface anisotropy of NbTe_2. Finally, non-saturating linear magnetoresistance up to 50 T is observed and attributed to the quantum limit transport.
文摘Despite the long availability of a traditional prophylactic vaccine containing the HBV surface antigen(HBsA g) and aluminum adjuvant, nearly 10% of the population remains unable to generate an effective immune response. Previous studies have indicated that hepatitis B virus(HBV) PreS 2-S is abundant in T/B cell epitopes, which induces a stronger immune response than HBsA g, particularly in terms of cytotoxic T lymphocyte(CTL) reaction. In the current study, the HBV PreS 2-S gene encoding an extra26 amino acids(PreS 2 C-terminus) located at the N-terminus of HBsA g was cloned into the pV CH1300 expression vector. Pre S2-S expressed in the methylotrophic yeast, Hansenula polymorpha, was produced at a yield of up to 250 mg/L. Subsequent purification steps involved hydrophobic adsorption to colloidal silica, ion-exchange chromatography and density ultracentrifugation. The final product was obtained with a total yield of ~15% and purity of ~99%. In keeping with previous studies, ~22 nm viruslike particles were detected using electron microscopy. The generated PreS 2-S antigen will be further studied for efficacy and safty in animals.
基金supported by the Natural Science Foundation of Jiangsu Province (Grant No. BK20240170)Open fund by Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (KHK2203)+2 种基金the Jiangsu Meteorological Bureau Youth Fund (KQ202314)the Fundamental Research Funds for the Central Universities (2024300330)Jiangsu Collaborative Innovation Center for Climate Change。
文摘Increasing the urban tree cover percentage(TCP) is widely recognized as an efficient way to mitigate the urban heat island effect. The cooling efficiency of urban trees can be either enhanced or attenuated on hotter days, depending on the physiological response of urban trees to rising ambient temperature. However, the response of urban trees' cooling efficiency to rising urban temperature remains poorly quantified for China's cities. In this study, we quantify the response of urban trees' cooling efficiency to rising urban temperature at noontime [~1330 LT(local time), LT=UTC+8] in 17summers(June, July, and August) from 2003–19 in 70 economically developed cities of China based on satellite observations. The results show that urban trees have stronger cooling efficiency with increasing temperature, suggesting additional cooling benefits provided by urban trees on hotter days. The enhanced cooling efficiency values of urban trees range from 0.002 to 0.055℃ %-1 per 1℃ increase in temperature across the selected cities, with larger values for the lowTCP-level cities. The response is also regulated by background temperature and precipitation, as the additional cooling benefit tends to be larger in warmer and wetter cities at the same TCP level. The positive response of urban trees' cooling efficiency to rising urban temperature is explained mainly by the stronger evapotranspiration of urban trees on hotter days.These results have important implications for alleviating urban heat risk by utilizing urban trees, particularly considering that extreme hot days are becoming more frequent in cities under global warming.
文摘The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis.Expanding enzyme catalysis to a larger substrate scope and greater variety of chemical reactions and tuning the microenvironment surrounding enzyme molecules to achieve high enzyme performance are urgently needed.In this account,we focus on our efforts using the de novo approach to synthesis hybrid enzyme catalysts that can address these two challenges and the structure-function relationship is discussed to reveal the principles of designing hybrid enzyme catalysts.We hope that this account will promote further efforts toward fundamental research and wide applications of designed enzyme hybrid catalysts for expanding biocatalysis.
基金National Natural Science Foundation of China(Nos.51672184,81622032 and 81501858)the Jiangsu Innovation and Entrepreneurship Programthe Principal Project of Natural Science Research of Jiangsu Higher Education Institutions(No.17KJA180011) for supporting this work
文摘Flexible and environment-responsive materials are essential for a large number of applications from artificial skin to wearable devices. The present study develops a flexible, ultra-low cost conductive hybrid elastomer(CHE), which possesses high responsive capabilities to stress/strain and humidity. CHE was composed of polydimethylsiloxane(PDMS) and starch hydrogel(SH), enabling great elasticity(56 kPa),high conductivity(10^(-2)S/m) and high sensitivity to external stimuli(gauge factor of CHE under stress and strain are 0.71 and 2.22, respectively, and sensitivity to humidity is 1.2 × 10^(-6)S/m per RH%). These properties render CHE a promising candidate for artificial skin and wearable electronics applications of continuously monitoring environmental information.
基金supported by the National Natural Science Foundation of China(62173329)。
文摘Online traffic simulation that feeds from online information to simulate vehicle movement in real-time has recently seen substantial advancement in the development of intelligent transportation systems and urban traffic management.It has been a challenging problem due to three aspects:1)The diversity of traffic patterns due to heterogeneous layouts of urban intersections;2)The nature of complex spatiotemporal correlations;3)The requirement of dynamically adjusting the parameters of traffic models in a real-time system.To cater to these challenges,this paper proposes an online traffic simulation framework called automated urban traffic operation simulation via meta-learning(AUTOSIM).In particular,simulation models with various intersection layouts are automatically generated using an open-source simulation tool based on static traffic geometry attributes.Through a meta-learning technique,AUTOSIM enables an automated learning process for dynamic model settings of traffic scenarios featured with different spatiotemporal correlations.Besides,AUTOSIM is capable of adapting traffic model parameters according to dynamic traffic information in real-time by using a meta-learner.Through computational experiments,we demonstrate the effectiveness of the meta-learningbased framework that is capable of providing reliable supports to real-time traffic simulation and dynamic traffic operations.
文摘Palladium(Pd)‐based catalysts are essential to drive high‐performance Suzuki coupling reactions,which are powerful tools for the synthesis of functional organic compounds.Herein,we developed a solution‐rapid‐annealing process to stabilize nitrogen‐mesoporous carbon supported Pd single‐atom/cluster(Pd/NMC)material,which provided a catalyst with superior performance for Suzuki coupling reactions.In comparison with commercial palladium/carbon(Pd/C)catalysts,the Pd/NMC catalyst exhibited significantly boosted activity(100%selectivity and 95%yield)and excellent stability(almost no decay in activity after 10 reuse cycles)for the Suzuki coupling reactions of chlorobenzenes,together with superior yield and excellent selectivity in the fields of the board scope of the reactants.Moreover,our newly developed rapid annealing process of precursor solutions is applied as a generalized method to stabilize metal clusters(e.g.Pd,Pt,Ru),opening new possibilities in the construction of efficient highly dispersed metal atom and sub‐nanometer cluster catalysts with high performance.
文摘From theories and cases,this paper analyzed current situation of subsidy policy system for agricultural natural disasters in China.Improvement and perfection of subsidy policies for agricultural natural disasters need clear classification and additional special subsidy plan.It is recommended to improve and perfect subsidy policy system for agricultural natural disasters through attaching importance to scientific studies on reduction and prevention of agricultural disasters and gradually supplementing agricultural disaster reduction and prevention system.
文摘BACKGROUND Vascular injury is a rare complication of femoral shaft fractures,and rupture of the deep femoral artery is more difficult to diagnose because of its anatomical location and symptoms.Despite its low incidence,deep femoral artery rupture can lead to life-threatening outcomes,such as compartment syndrome,making early identification and diagnosis critical.CASE SUMMARY A 45-year-old male patient was admitted to our hospital due to right lower limb trauma in a car accident,with complaints of severe pain and swelling on his right thigh.X-ray demonstrated a right femoral shaft fracture.During preparation for emergency surgery,his blood pressure and blood oxygen saturation dropped,and sensorimotor function was lost.Computed tomography angiography was performed immediately to confirm the diagnosis of rupture of the deep femoral artery and compartment syndrome,so fasciotomy and vacuum-assisted closure were performed.Rhabdomyolysis took place after the operation and the patient was treated with appropriate electrolyte correction and diuretic therapy.Twenty days after the fasciotomy,treatment with the Hoffman Type II External Fixation System was planned,but it was unable to be immobilized internally based on a new esophageal cancer diagnosis.We kept the external fixation for 1 year,and 3 years of follow-up showed improvement of the patient’s overall conditions and muscle strength.CONCLUSION For patients with thigh swelling,pain,anemia,and unstable vital signs,anterior femoral artery injury should be highly suspected.Once diagnosed,surgical treatment should be performed immediately and complications of artery rupture must be suspected and addressed in time.
基金supported by the Japan Society for the Promotion of Science(JSPS)(KAKENHI grant nos.21H05034 and 21F21096 to J.F.M.,and 19H03250 to N.Y.)。
文摘Rice is a staple food for half of the world’s population,but it is a poor dietary source of calcium(Ca)due to the low concentration.It is an important issue to boost Ca concentration in this grain to improve Ca deficiency risk,but the mechanisms underlying Ca accumulation are poorly understood.Here,we obtained a rice(Oryza sativa)mutant with high shoot Ca accumulation.The mutant exhibited 26%-53% higher Ca in shoots than did wild-type rice(WT)at different Ca supplies.Ca concentration in the xylem sap was 36% higher in the mutant than in the WT.There was no difference in agronomic traits between the WT and mutant,but the mutant showed 25% higher Ca in the polished grain compared with the WT.Map-based cloning combined with a complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene,OsK5.2,belonging to the Shaker-like K+channel family.OsK5.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels,but upregulated by low K.Immunostaining showed that OsK5.2 was mainly expressed in the pericycle of the roots.Taken together,our results revealed a novel role for OsK5.2 in Ca translocation in rice,and will be a good target for Ca biofortification in rice.
基金supported by the National Key Research and Development Program of China(2022YFB3804600,2021YFF0701800)the National Natural Science Foundation of China(U20A20254,52072253)+4 种基金the Science and Technology Development Fund,Macao SAR(0118/2023/RIA2)Collaborative Innovation Center of Suzhou Nano Science and Technology,Suzhou Key Laboratory of Nanotechnology and Biomedicine,the Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20211544)the 111 Project,Joint International Research Laboratory of Carbon-Based Functional Materials and Devices,Medical Research Project of Jiangsu Province(ZD2022024)New Cornerstone Science Foundation through the XPLORER PRIZE,and Key Laboratory of Structural Deformities in Children of Suzhou(SZS2022018)N.Y.was supported by the Macao Young Scholars Program and the Jiangsu Funding Program for Excellent Postdoctoral Talent.
文摘Implant-related osteomyelitis is a formidable hurdle in the clinical setting and is characterized by inflammation,infection,and consequential bone destruction.Therefore,effective reactive oxygen species(ROS)scavenging,bacterial killing,and subsequent bone tissue repair are urgently needed for the treatment of difficult-to-heal osteomyelitis.Herein,we utilized the eddy-thermal effect of magnesium(Mg)implants under an alternating magnetic field(AMF)for the controlled release of H_(2)gas and ions(OH^(-)and Mg^(2+))for the treatment of osteomyelitis.H_(2)released by Mg rods under AMFs effectively scavenged cytotoxic ROS,exhibiting anti-inflammatory effects and consequently disrupting the environment of bacterial infections.In addition,the OH-hindered the energy metabolism of bacteria by effectively neutralizing protons within the microenvironment.Moreover,H_(2)impaired the permeability of bacterial membranes and expedited the damage induced by OH-.This synergistic AMF-induced H_(2)and proton depletion treatment approach not only killed both gram-negative and gram-positive bacteria but also effectively treated bacterial infections(abscesses and osteomyelitis).Moreover,Mg^(2+)released from the Mg rods enhanced and accelerated the process of bone osteogenesis.Overall,our work cleverly exploited the eddy-thermal effect and chemical activity of Mg implants under AMFs,aiming to eliminate the inflammatory environment and combat bacterial infections by the simultaneous release of H_(2),OH^(-),and Mg^(2+),thereby facilitating tissue regeneration.This therapeutic strategy achieved multiple benefits in one,thus presenting a promising avenue for clinical application.
文摘Catalysis plays an important role in human civilization and economic development.Every breakthrough related to the field of catalysis has great impact on our everyday lives.To achieve harmonious development between human and nature through developing chemical processes with environmental friendliness and atom economy,the improvement and innovation of catalytic systems have drawn great attention.Biocatalysis is the engine of life from the very beginning,which is the example that we always learn from,mimic,and aim to surpass in scientific research and industrial development.The goal of the development of biocatalysis technology is tomake our industrial manufacturing go undermild conditions as nature does.However,the current situation of biocatalysis application is still far from the ultimate aim,providing countless opportunities for further development.
基金financially supported by Anhui Province Key Research and Development Program(No.2022i01020002)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.22KJB180020)+2 种基金the Scientific Research Project of Anhui Polytechnic University(No.2022YQQ072)Anhui Provincial Higher Education Institutes(No.2023AH050941)the Advanced Fiber Materials Engineering Research Center of Anhui Province(No.2023AFMC20)。
文摘Waterborne microbial contamination represents a significant global environmental challenge.Microorganisms can induce water pollution,precipitating infectious disease outbreaks and posing a substantial threat to human health.Currently,the excessive use of conventional antibiotics has resulted in the emergence of bacterial resistance,posing a challenge to the design and development of antimicrobial agents with high antibacterial efficacy in diverse water such as rivers,lakes,and wastewater from microbiology laboratories.Bimetallic nanomaterials,that amalgamate the distinctive characteristics of diverse metals,are garnering attention due to their synergistic properties.In this study,PVP-FeMo_(2)S_(3)(PVP:Polyvinylpyrrolidone)and PVP-ZnMo_(2)S_(3)nanocrystals were synthesized by a high-temperature reaction.The prepared nanocrystals exhibited excellent biocompatibility,regular morphology,long-term stability,and remarkable antibacterial e ects.The bacterial biofilms could be eradicated by the combination of photothermal(PTT)and photodynamic synergistic e ects of PVP-FeMo_(2)S_(3)and PVP-ZnMo_(2)S_(3)nanocrystals.PVP-ZnMo_(2)S_(3)nanocrystals exhibited enhanced PTT conversion efficiency for water treatment.Remarkably,even after 20 cycles of recycling,PVP-ZnMo_(2)S_(3)retained substantial antibacterial e cacy.The excellent biosafety of PVP-ZnMo_(2)S_(3)further guarantees the safety of water quality post-treatment.Thus,PVP-ZnMo_(2)S_(3)bimetallic nanocrystals can serve as a novel antimicrobial agent for the treatment of microbial contamination in water.This study offers a potential strategy to mitigate secondary water pollution and prevent the development of drug resistance among pathogenic microorganisms.
基金supported by National Natural Science Foundation of China under Grant 51977066。
文摘Since the fault dynamic of droop-controlled inverter is different from synchronous generators (SGs), protection devices may become invalid, and the fault overcurrent may damage power electronic devices and threaten the safety of the microgrid. Therefore, it is imperative to conduct a comprehensive fault analysis of the inverter to guide the design of protection schemes. However, due to the complexity of droop control strategy, existing literatures have simplified asymmetric fault analysis of droop-controlled inverters to varying degrees. Therefore, accurate fault analysis of a droop-controlled inverter is needed. In this paper, by analyzing the control system, an accurate fault model is established. Based on this, a calculation method for instantaneous asymmetrical fault current is proposed. In addition, the current components and current characteristics are analyzed. It was determined that fault currents are affected by control loops, fault types, fault distance and nonlinear limiters. In particular, the influences of limiters on the fault model, fault current calculation and fault current characteristics were analyzed. Through detailed analysis, it was found that dynamics of the control loop cannot be ignored, the fault type and fault distance determine fault current level, and part of the limiters will totally change the fault current trend. Finally, calculation and experimental results verify the correctness of the proposed method.
文摘Objective:To evaluate the therapeutic effect of dynamic fixation with a hook plate-double loop plate internal fixation system for high-energy acromioclavicular joint complex dislocation(denoted as HACD,i.e.,Rockwood type III-V dislocation).Methods:Fifty-eight patients with HACD were selected and evenly divided by drawing lots.The experimental group underwent ligament reconstruction treatment,while the reference group received hook plate fixation treatment.The efficacy and other indicators were compared between the groups.Results:The overall effective rates between the groups were similar(P>0.05).The experimental group had a longer surgical duration,lower pain scores at 6 months postoperatively,higher shoulder joint function scores,and a lower complication rate,with P<0.05 when compared between groups.Conclusion:The effectiveness of the double loop plate internal fixation system ligament reconstruction treatment for patients with HACD is comparable to that of hook plate fixation treatment.Although the surgical duration is slightly longer,postoperative pain is milder,facilitating the recovery of shoulder joint function in patients and offering higher safety.
