The elevational distributions of bacterial communities in natural mountain forests,especially along large elevational gradients,have been studied for many years.However,the distributional patterns that underlie variat...The elevational distributions of bacterial communities in natural mountain forests,especially along large elevational gradients,have been studied for many years.However,the distributional patterns that underlie variations in soil bacterial communities along small-scale elevational gradients in urban ecosystems are not yet well understood.Using Illumina MiSeq DNA sequencing,we surveyed soil bacterial communities at three elevations on Zijin Mountain in Nanjing City:the hilltop(300 m a.s.l.),the hillside(150 m a.s.l.),and the foot of the hill(0 m a.s.l.).The results showed that edaphic properties differed significantly with elevation.Bacterial community composition,rather than alpha diversity,strongly differed among the three elevations(Adonis:R2=0.12,P<0.01).Adonis and DistLM analyses demonstrated that bacterial community composition was highly correlated with soil pH,elevation,total nitrogen(TN),and dissolved organic carbon(DOC).The degree scores,betweenness centralities,and composition of keystone species were distinct among the elevations.These results demonstrate strong elevational partitioning in the distributions of soil bacterial communities along the gradient on Zijin Mountain.Soil pH and elevation together drove the smallscale elevational distribution of soil bacterial communities.This study broadens our understanding of distribution patterns and biotic co-occurrence associations of soil bacterial communities from large elevational gradients to short elevational gradients.展开更多
We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS da...We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS data,we updated a bioinformatic pipeline to enhance fungal taxonomic resolution.In forests,fungal sporocarps house the diverse fungicolous fungi;however,the relationships of sporocarps and associated fungal communities are rarely explored in agroecosystems.In a corn field near Gongzhuling City,Jilin Province,China,we found an epigeous sporocarp with agaricoid morphology that could grow next to the living corn plants.Using PacBio metabarcoding combined with an updated bioinformatic pipeline,we surveyed the fungal community profile along its cap,rhizomorph and hyphosphere soil at a much-improved taxonomic resolution.We identified the sporocarp,at a high probability,as Agrocybe dura,and this mushroom was significantly negatively correlated with Trichoderma hamatum and T.harzianum in the co-occurrence network.Fungal diversity in hyphosphere habitat was significantly higher than that in cap and rhizomorph habitats.Consistent with the pattern in fungal diversity,the node number,edge number,network diameter and average degree were significantly higher in hyphosphere habitat than other habitats.However,both the negative and positive cohesion were significantly higher in rhizomorph habitat than other habitats.Moreover,the z-c plot identified A.dura as the only network hub,linking multiple fungal species.The results give us a glimpse of the ecological relevance of saprobic mushrooms across the extensive northeastern black soil region of China.Our findings will aid in the assessment and forecasting of fungal diversity hotspots and their relationships with soil fertility in the‘Golden Corn Belt’of northeast China.展开更多
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB15010101)the National Natural Science Foundation of China(41907039)the China Biodiversity Observation Networks(Sino BON).
文摘The elevational distributions of bacterial communities in natural mountain forests,especially along large elevational gradients,have been studied for many years.However,the distributional patterns that underlie variations in soil bacterial communities along small-scale elevational gradients in urban ecosystems are not yet well understood.Using Illumina MiSeq DNA sequencing,we surveyed soil bacterial communities at three elevations on Zijin Mountain in Nanjing City:the hilltop(300 m a.s.l.),the hillside(150 m a.s.l.),and the foot of the hill(0 m a.s.l.).The results showed that edaphic properties differed significantly with elevation.Bacterial community composition,rather than alpha diversity,strongly differed among the three elevations(Adonis:R2=0.12,P<0.01).Adonis and DistLM analyses demonstrated that bacterial community composition was highly correlated with soil pH,elevation,total nitrogen(TN),and dissolved organic carbon(DOC).The degree scores,betweenness centralities,and composition of keystone species were distinct among the elevations.These results demonstrate strong elevational partitioning in the distributions of soil bacterial communities along the gradient on Zijin Mountain.Soil pH and elevation together drove the smallscale elevational distribution of soil bacterial communities.This study broadens our understanding of distribution patterns and biotic co-occurrence associations of soil bacterial communities from large elevational gradients to short elevational gradients.
基金supported by the National Program on Key Basic Research Project(Grant No.2022YFD1500202)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA28020202)the National Natural Science Foundation of China(Grant No.42277308).
文摘We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS data,we updated a bioinformatic pipeline to enhance fungal taxonomic resolution.In forests,fungal sporocarps house the diverse fungicolous fungi;however,the relationships of sporocarps and associated fungal communities are rarely explored in agroecosystems.In a corn field near Gongzhuling City,Jilin Province,China,we found an epigeous sporocarp with agaricoid morphology that could grow next to the living corn plants.Using PacBio metabarcoding combined with an updated bioinformatic pipeline,we surveyed the fungal community profile along its cap,rhizomorph and hyphosphere soil at a much-improved taxonomic resolution.We identified the sporocarp,at a high probability,as Agrocybe dura,and this mushroom was significantly negatively correlated with Trichoderma hamatum and T.harzianum in the co-occurrence network.Fungal diversity in hyphosphere habitat was significantly higher than that in cap and rhizomorph habitats.Consistent with the pattern in fungal diversity,the node number,edge number,network diameter and average degree were significantly higher in hyphosphere habitat than other habitats.However,both the negative and positive cohesion were significantly higher in rhizomorph habitat than other habitats.Moreover,the z-c plot identified A.dura as the only network hub,linking multiple fungal species.The results give us a glimpse of the ecological relevance of saprobic mushrooms across the extensive northeastern black soil region of China.Our findings will aid in the assessment and forecasting of fungal diversity hotspots and their relationships with soil fertility in the‘Golden Corn Belt’of northeast China.
