Quantitative phase imaging(QPI)is a label-free technique providing both morphology and quantitative biophysical information in biomedicine.However,applying such a powerful technique to in vivo pathological diagnosis r...Quantitative phase imaging(QPI)is a label-free technique providing both morphology and quantitative biophysical information in biomedicine.However,applying such a powerful technique to in vivo pathological diagnosis remains challenging.Multi-core fiber bundles(MCFs)enable ultra-thin probes for in vivo imaging,but current MCF imaging techniques are limited to amplitude imaging modalities.We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length.The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side,enabling digital refocusing in a multi-layer sample without any mechanical movement.The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF.With the proposed imaging modality,three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope,promising widespread clinical applications.展开更多
基金Deutsche Forschungsgemeinschaft(DFG)grant CZ55/40-1Tsinghua Scholarship for Overseas Graduate Studies grant 2020023+1 种基金European Union’s Horizon 2020 research and innovation programs No.953121(project FLAMIN-GO)Open Access funding enabled and organized by Projekt DEAL。
文摘Quantitative phase imaging(QPI)is a label-free technique providing both morphology and quantitative biophysical information in biomedicine.However,applying such a powerful technique to in vivo pathological diagnosis remains challenging.Multi-core fiber bundles(MCFs)enable ultra-thin probes for in vivo imaging,but current MCF imaging techniques are limited to amplitude imaging modalities.We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length.The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side,enabling digital refocusing in a multi-layer sample without any mechanical movement.The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF.With the proposed imaging modality,three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope,promising widespread clinical applications.
