The quarantine bacterium Xanthomonas fragariae causes angular leaf spots on strawberry.Its population structure was recently found to be divided into four(sub)groups resulting from two distinct main groups.Xanthomonas...The quarantine bacterium Xanthomonas fragariae causes angular leaf spots on strawberry.Its population structure was recently found to be divided into four(sub)groups resulting from two distinct main groups.Xanthomonas arboricola pv.fragariae causes bacterial leaf blight,but the bacterium has an unclear virulence status on strawberry.In this study,we use comparative genomics to provide an overview of the genomic variations of a set of 58 X.fragariae and five X.arboricola pv.fragariae genomes with a focus on virulence-related proteins.Structural differences within X.fragariae such as differential plasmid presence and large-scale genomic rearrangements were observed.On the other hand,the virulence-related protein repertoire was found to vary greatly at the interspecies level.In three out of five sequenced X.arboricola pv.fragariae strains,the major part of the Hrp typeⅢsecretion system was lacking.An inoculation test with strains from all four X.fragariae(sub)groups and X.arboricola pv.fragariae resulted in an interspecies difference in symptom induction since no symptoms were observed on the plants inoculated with X.arboricola pv.fragariae.Our analysis suggests that all X.fragariae(sub)groups are pathogenic on strawberry plants.On the other hand,the first genomic investigations of X.arboricola pv.fragariae revealed a potential lack of certain key virulence-related factors which may be related to the difficulties to reproduce symptoms on strawberry and could question the plant-host interaction of the pathovar.展开更多
基金Funding was provided by the European Union Seventh Framework(FP7/2007–2013)under the grant agreement no.613678(DROPSA)The EDGAR platform is financially supported by the BMBF grant FKZ 031A533 within the de.NBI network.
文摘The quarantine bacterium Xanthomonas fragariae causes angular leaf spots on strawberry.Its population structure was recently found to be divided into four(sub)groups resulting from two distinct main groups.Xanthomonas arboricola pv.fragariae causes bacterial leaf blight,but the bacterium has an unclear virulence status on strawberry.In this study,we use comparative genomics to provide an overview of the genomic variations of a set of 58 X.fragariae and five X.arboricola pv.fragariae genomes with a focus on virulence-related proteins.Structural differences within X.fragariae such as differential plasmid presence and large-scale genomic rearrangements were observed.On the other hand,the virulence-related protein repertoire was found to vary greatly at the interspecies level.In three out of five sequenced X.arboricola pv.fragariae strains,the major part of the Hrp typeⅢsecretion system was lacking.An inoculation test with strains from all four X.fragariae(sub)groups and X.arboricola pv.fragariae resulted in an interspecies difference in symptom induction since no symptoms were observed on the plants inoculated with X.arboricola pv.fragariae.Our analysis suggests that all X.fragariae(sub)groups are pathogenic on strawberry plants.On the other hand,the first genomic investigations of X.arboricola pv.fragariae revealed a potential lack of certain key virulence-related factors which may be related to the difficulties to reproduce symptoms on strawberry and could question the plant-host interaction of the pathovar.
