Osteoporosis is a prevalent skeletal condition characterized by reduced bone mass and strength,leading to increased fragility.Buqi-Tongluo(BQTL)decoction,a traditional Chinese medicine(TCM)prescription,has yet to be f...Osteoporosis is a prevalent skeletal condition characterized by reduced bone mass and strength,leading to increased fragility.Buqi-Tongluo(BQTL)decoction,a traditional Chinese medicine(TCM)prescription,has yet to be fully evaluated for its potential in treating bone diseases such as osteoporosis.To investigate the mechanism by which BQTL decoction inhibits osteoclast differentiation in vitro and validate these findings through in vivo experiments.We employed MTS assays to assess the potential proliferative or toxic effects of BQTL on bone marrow macrophages(BMMs)at various concentrations.TRAc P experiments were conducted to examine BQTL's impact on osteoclast differentiation.RT-PCR and Western blot analyses were utilized to evaluate the relative expression levels of osteoclast-specific genes and proteins under BQTL stimulation.Finally,in vivo experiments were performed using an osteoporosis model to further validate the in vitro findings.This study revealed that BQTL suppressed receptor activator of NF-κB ligand(RANKL)-induced osteoclastogenesis and osteoclast resorption activity in vitro in a dose-dependent manner without observable cytotoxicity.The inhibitory effects of BQTL on osteoclast formation and function were attributed to the downregulation of NFATc1 and c-fos activity,primarily through attenuation of the MAPK,NF-κB,and Calcineurin signaling pathways.BQTL's inhibitory capacity was further examined in vivo using an ovariectomized(OVX)rat model,demonstrating a strong protective effect against bone loss.BQTL may serve as an effective therapeutic TCM for the treatment of postmenopausal osteoporosis and the alleviation of bone loss induced by estrogen deficiency and related conditions.展开更多
Mobile robots are often subject to multiplicative noise in the target tracking tasks,where the multiplicative measurement noise is correlated with additive measurement noise.In this paper,first,a correlation multiplic...Mobile robots are often subject to multiplicative noise in the target tracking tasks,where the multiplicative measurement noise is correlated with additive measurement noise.In this paper,first,a correlation multiplicative measurement noise model is established.It is able to more accurately represent the measurement error caused by the distance sensor dependence state.Then,the estimated performance mismatch problem of Cubature Kalman Filter(CKF)under multiplicative noise is analyzed.An improved Gaussian filter algorithm is introduced to help obtain the CKF algorithm with correlated multiplicative noise.In practice,the model parameters are unknown or inaccurate,especially the correlation of noise is difficult to obtain,which can lead to a decrease in filtering accuracy or even divergence.To address this,an adaptive CKF algorithm is further provided to achieve reliable state estimation for the unknown noise correlation coefficient and thus the application of the CKF algorithm is extended.Finally,the estimated performance is analyzed theoretically,and the simulation study is conducted to validate the effectiveness of the proposed algorithm.展开更多
Fractures have an extraordinarily negative impact on individuals'quality of life and functional status.Nonunion or disability of fracture is a major health issue with important clinical,social,and economic implica...Fractures have an extraordinarily negative impact on individuals'quality of life and functional status.Nonunion or disability of fracture is a major health issue with important clinical,social,and economic implications.1 Mesenchymal stem cells(MSCs)play an indispensable role in the initiation of the fracture repair process including the formation of a callus which is replaced by new bone.The use of MSCs in the treatment of fractures is very attractive as they can reduce the time of healing and occurrence of nonunion.展开更多
1.Background Conjunctival inclusion cyst is formed by hyperplasia and degeneration of epithelial cells implanted in subconjunctival area,which is common after ocular trauma and eye surgery.1 It can also be found in St...1.Background Conjunctival inclusion cyst is formed by hyperplasia and degeneration of epithelial cells implanted in subconjunctival area,which is common after ocular trauma and eye surgery.1 It can also be found in Stevens-Johnson syndrome(SJS),which results in extensive and severe symblepharon and the formation of cysts by encapsulating residual conjunctival epithelium on the fornix.2 The previous treatments of conjunctival inclusion cyst include complete excision surgically,cyst aspiration,foam sclerotherapy after cyst aspiration,3 and fornix reconstruction with cyst marsupialization,4 which owned many advantages but a similar surgery was only presented in a few case reports.In our clinical practice,the cyst wall was used for marsupialization in four patients suffering from both large conjunctival inclusion cyst and severe symblepharon.Owing to the difficulty of the treatment of SJS,the poor therapeutic effect of the traditional method,and the small number of articles on fornix reconstruction with conjunctival inclusion cyst marsupialization,such cases are summarized in the present study to introduce the clinical application of this operation for reference.展开更多
Purpose:To establish a comprehensive treatment strategy and evaluate the efficacy of combination of anti-vascular endothelial growth factor(VEGF)injection,pars plana vitrectomy(PPV),endoscopic pan-retinal photocoagula...Purpose:To establish a comprehensive treatment strategy and evaluate the efficacy of combination of anti-vascular endothelial growth factor(VEGF)injection,pars plana vitrectomy(PPV),endoscopic pan-retinal photocoagulation(PRP),and endoscopic cyclophotocoagulation(ECP)surgery for neovascular glaucoma(NVG)patients.Methods:This retrospective study included 30 patients(30 eyes)who were suffering from NVG and treated with PPV&PRP&ECP(ECP group,16 eyes),or Ahmed glaucoma valve implantation(Ahmed group,14 eyes).The intraocular pressure(IOP),number of postoperative anti-glaucoma medications,best-corrected visual acuity(BCVA),successful rate of surgery,and postoperative complications were recorded and statistically analyzed at the time points of preoperative,1-day,1-month,3-months,6-months,and 12-months after operation.Results:An obvious reduction in IOP and number of postoperative anti-glaucoma medications were observed in both the ECP group and Ahmed group after operation(P<0.05),and the ECP group showed a significantly lower IOP compared to the Ahmed group at the 6-months(P=0.014)and 12-months(P=0.047)postoperative time points,while there was no significant difference of medication number between the two groups except for 1-day after surgery.The BCVA showed no marked difference between the two groups preoperatively and postoperatively(P>0.05),while it was significantly improved in ECP group at 3-months(P=0.001),6-months(P=0.004),and 12-months(P=0.010)time points comparing with preoperative BCVA.The surgical success rates in ECP group were also slightly higher than Ahmed group.And the complications after operation showed no marked differences.Conclusions:The comprehensive treatment of PPV,endoscopic PRP,and ECP surgery for NVG patients after antiVEGF injection can control IOP effectively and be friendly to patients’BCVA without obvious serious complications throughout a 12-months follow-up period.展开更多
基金supported by the Natural Science Foundation of Guangdong Province,China(No.2021A1515012168)the Administration of Traditional Chinese Medicine of Guangdong Province,China(Nos.20221146 and 20241091)+5 种基金the Basic and Applied Basic Research Fund Project in Guangdong Province,China(No.2020A1515110948)the Basic and Applied Basic Research in Jointly Funded Projects of City Schools(Institutes)Projects,China(Nos.202201020500 and 202201020295)the Project of Guangzhou Science and Technology Department,China(No.202102021040)the Guangzhou Science and Technology Plan Project,China(No.2023B03J0379)the Chinese Society of Traditional Chinese Medicine Youth Talent Lifting Project(No.2022-QNRC2-B11)the Hospital Young and Middle-aged Key Talent Cultivation Project of The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine(2023-10)。
文摘Osteoporosis is a prevalent skeletal condition characterized by reduced bone mass and strength,leading to increased fragility.Buqi-Tongluo(BQTL)decoction,a traditional Chinese medicine(TCM)prescription,has yet to be fully evaluated for its potential in treating bone diseases such as osteoporosis.To investigate the mechanism by which BQTL decoction inhibits osteoclast differentiation in vitro and validate these findings through in vivo experiments.We employed MTS assays to assess the potential proliferative or toxic effects of BQTL on bone marrow macrophages(BMMs)at various concentrations.TRAc P experiments were conducted to examine BQTL's impact on osteoclast differentiation.RT-PCR and Western blot analyses were utilized to evaluate the relative expression levels of osteoclast-specific genes and proteins under BQTL stimulation.Finally,in vivo experiments were performed using an osteoporosis model to further validate the in vitro findings.This study revealed that BQTL suppressed receptor activator of NF-κB ligand(RANKL)-induced osteoclastogenesis and osteoclast resorption activity in vitro in a dose-dependent manner without observable cytotoxicity.The inhibitory effects of BQTL on osteoclast formation and function were attributed to the downregulation of NFATc1 and c-fos activity,primarily through attenuation of the MAPK,NF-κB,and Calcineurin signaling pathways.BQTL's inhibitory capacity was further examined in vivo using an ovariectomized(OVX)rat model,demonstrating a strong protective effect against bone loss.BQTL may serve as an effective therapeutic TCM for the treatment of postmenopausal osteoporosis and the alleviation of bone loss induced by estrogen deficiency and related conditions.
基金supported by the National Natural Science Foundation of China(Nos.61773147 and 62033010)Zhejiang Provincial Nature Science Foundation of China(Nos.LR17F030005 and LZ21F030004)Key-Area Research and Development Program of Guangdong Province,china(No.2018B010107002)。
文摘Mobile robots are often subject to multiplicative noise in the target tracking tasks,where the multiplicative measurement noise is correlated with additive measurement noise.In this paper,first,a correlation multiplicative measurement noise model is established.It is able to more accurately represent the measurement error caused by the distance sensor dependence state.Then,the estimated performance mismatch problem of Cubature Kalman Filter(CKF)under multiplicative noise is analyzed.An improved Gaussian filter algorithm is introduced to help obtain the CKF algorithm with correlated multiplicative noise.In practice,the model parameters are unknown or inaccurate,especially the correlation of noise is difficult to obtain,which can lead to a decrease in filtering accuracy or even divergence.To address this,an adaptive CKF algorithm is further provided to achieve reliable state estimation for the unknown noise correlation coefficient and thus the application of the CKF algorithm is extended.Finally,the estimated performance is analyzed theoretically,and the simulation study is conducted to validate the effectiveness of the proposed algorithm.
基金supported by the National Natural Science Foundation of China(No.81871778,81874000,82272505).
文摘Fractures have an extraordinarily negative impact on individuals'quality of life and functional status.Nonunion or disability of fracture is a major health issue with important clinical,social,and economic implications.1 Mesenchymal stem cells(MSCs)play an indispensable role in the initiation of the fracture repair process including the formation of a callus which is replaced by new bone.The use of MSCs in the treatment of fractures is very attractive as they can reduce the time of healing and occurrence of nonunion.
文摘1.Background Conjunctival inclusion cyst is formed by hyperplasia and degeneration of epithelial cells implanted in subconjunctival area,which is common after ocular trauma and eye surgery.1 It can also be found in Stevens-Johnson syndrome(SJS),which results in extensive and severe symblepharon and the formation of cysts by encapsulating residual conjunctival epithelium on the fornix.2 The previous treatments of conjunctival inclusion cyst include complete excision surgically,cyst aspiration,foam sclerotherapy after cyst aspiration,3 and fornix reconstruction with cyst marsupialization,4 which owned many advantages but a similar surgery was only presented in a few case reports.In our clinical practice,the cyst wall was used for marsupialization in four patients suffering from both large conjunctival inclusion cyst and severe symblepharon.Owing to the difficulty of the treatment of SJS,the poor therapeutic effect of the traditional method,and the small number of articles on fornix reconstruction with conjunctival inclusion cyst marsupialization,such cases are summarized in the present study to introduce the clinical application of this operation for reference.
基金supported by the Clinical diagnosis and treatment technology and translational application in Beijing (Z211100002921049).
文摘Purpose:To establish a comprehensive treatment strategy and evaluate the efficacy of combination of anti-vascular endothelial growth factor(VEGF)injection,pars plana vitrectomy(PPV),endoscopic pan-retinal photocoagulation(PRP),and endoscopic cyclophotocoagulation(ECP)surgery for neovascular glaucoma(NVG)patients.Methods:This retrospective study included 30 patients(30 eyes)who were suffering from NVG and treated with PPV&PRP&ECP(ECP group,16 eyes),or Ahmed glaucoma valve implantation(Ahmed group,14 eyes).The intraocular pressure(IOP),number of postoperative anti-glaucoma medications,best-corrected visual acuity(BCVA),successful rate of surgery,and postoperative complications were recorded and statistically analyzed at the time points of preoperative,1-day,1-month,3-months,6-months,and 12-months after operation.Results:An obvious reduction in IOP and number of postoperative anti-glaucoma medications were observed in both the ECP group and Ahmed group after operation(P<0.05),and the ECP group showed a significantly lower IOP compared to the Ahmed group at the 6-months(P=0.014)and 12-months(P=0.047)postoperative time points,while there was no significant difference of medication number between the two groups except for 1-day after surgery.The BCVA showed no marked difference between the two groups preoperatively and postoperatively(P>0.05),while it was significantly improved in ECP group at 3-months(P=0.001),6-months(P=0.004),and 12-months(P=0.010)time points comparing with preoperative BCVA.The surgical success rates in ECP group were also slightly higher than Ahmed group.And the complications after operation showed no marked differences.Conclusions:The comprehensive treatment of PPV,endoscopic PRP,and ECP surgery for NVG patients after antiVEGF injection can control IOP effectively and be friendly to patients’BCVA without obvious serious complications throughout a 12-months follow-up period.
