BACKGROUND:The lack of a stable,easy-to-operate animal model for severe trauma has hindered the research progress.The aim of this study is to develop a mouse model that replicates the pathophysiological conditions of ...BACKGROUND:The lack of a stable,easy-to-operate animal model for severe trauma has hindered the research progress.The aim of this study is to develop a mouse model that replicates the pathophysiological conditions of severe trauma,providing a reliable research tool.METHODS:Male C57BL/6J mice(aged 8-10 weeks and weighting approximately 20 g)were used to establish the severe trauma model.Under anesthesia,a midshaft femoral fracture was created and packed with sterile cotton.A midline incision was made from the inguinal region to the sternum,exposing the abdominal organs for 30 min.The right femoral artery was cannulated to induce controlled blood loss at 30%,35%,40%,and 50%of the total blood volume.Survival rates were monitored for 24 h post-induction.In the mice that experienced 30%blood loss,the mean arterial pressure,body temperature,blood gas parameters,peripheral blood inflammatory markers,and major organ pathological changes were assessed.RESULTS:Mice with femoral fractures,soft tissue injuries,abdominal organ exposure,and 30%blood loss exhibited stable survival rates.Increased blood loss significantly reduced survival rates.Mean arterial pressure decreased initially,recovering within 0-15 min and returning to baseline by 50 min.Similarly,the body temperature decreased initially and gradually recovered to baseline within 50 min.Levels of peripheral blood inflammatory markers remained elevated for 12 h post-injury.Distant organs,including intestines,lungs,liver,spleen and kidneys,displayed varying degrees of injury.CONCLUSION:The established mouse model replicates the pathophysiological responses to severe trauma,indicating stability and reproducibility,which could be an useful tool for further trauma research.展开更多
Objective Many studies have revealed the crucial roles of miRNA in multiple human cancers,including lung adenocarcinoma(LUAD).In this study,we sought to explore new miRNA-mRNA pairs that are associated with LUAD progn...Objective Many studies have revealed the crucial roles of miRNA in multiple human cancers,including lung adenocarcinoma(LUAD).In this study,we sought to explore new miRNA-mRNA pairs that are associated with LUAD prognosis.Methods A novel miRNA-mRNA regulatory network associated with prognosis in LUAD was identified and validated using the bioinformatic tools including OncomiR database,StarBase,miRnet,GEPIA2,UALCAN.Results Twenty key miRNAs were compiled after the analysis of the expression and prognostic value in OncomiR and StarBase.Targeted mRNAs of these key miRNAs were predicted in miRnet,and the resulting mRNAs were also analyzed for their prognostic values and expression patterns in GEPIA2 and UALCAN,respectively.Further expression correlation analysis was performed in StarBase.Subsequently,a new miRNA-mRNA network was built,of which each RNA pair showed negative expression correlation,opposite expression pattern,and prognostic value.Protein-protein interaction network was under construction for the mRNAs,and 19 hub genes were determined.Enrichment analysis showed that“Cell Cycle,Mitotic”was the most significantly enriched term.Then,a miRNA-hub gene sub-network was built.We selected and validated the regulatory relationship of some miRNA-hub pairs,including hsa-miR-1976/RFC2,hsa-let-7c-5p/RFC2,hsa-let-7c-5p/ESPL1,hsa-let-7c-5p/CDC25A,and hsa-miR-101-3p/KIF2C.Moreover,over-expression of hsa-miR-1976 and hsa-let-7c-5p resulted in significant cell cycle arrest.Conclusions Our results determined new prognosis-associated miRNA-mRNA pairs and might shed further light on the mechanism via which miRNA-mRNA network influences prognosis in LUAD.展开更多
Costume is not only an important carrier of human civilization,but also reflects the development level of material and spiritual civilization.In modern costume design,we have incorporated colorful cultural elements,wh...Costume is not only an important carrier of human civilization,but also reflects the development level of material and spiritual civilization.In modern costume design,we have incorporated colorful cultural elements,which are not only the embodiment of aesthetics but also the aesthetic expression based on culture.Printmaking art,as a kind of traditional art,has its unique style and characteristics.In teaching practice,we instruct students to integrate the creative spirit and formal elements of printmaking into costume design,to realize the inheritance and innovation of traditional culture.Under the background of globalization,we should be more committed to inheriting and carrying forward the excellent Chinese national culture and promoting the popularization and dissemination of traditional culture.Exploring the application of printmaking art in costume design through teaching practice aims to construct a new teaching mode,which provides a reference for the application of other traditional arts in modern design teaching.展开更多
A middle-aged man was diagnosed with myelodysplastic/myeloproliferative neoplasm-unclassifiable(MDS/MPN-U),with severe and extensive corticosteroid-resistant Sweet’s syndrome(SS)as the first manifestation,with eviden...A middle-aged man was diagnosed with myelodysplastic/myeloproliferative neoplasm-unclassifiable(MDS/MPN-U),with severe and extensive corticosteroid-resistant Sweet’s syndrome(SS)as the first manifestation,with evidence of clinical manifestations,pathological description,and laboratory evaluation.The skin lesions continued to spread despite treatment with systemic corticosteroids but were successfully treated with intravenous immunoglobulin(IVIG).展开更多
Purpose The study aimed to integrate genes associated with endoplasmic reticulum stress and immune responses to create a validated prognostic model for DLBCL.The research identified novel prognostic model and provided...Purpose The study aimed to integrate genes associated with endoplasmic reticulum stress and immune responses to create a validated prognostic model for DLBCL.The research identified novel prognostic model and provided insights for developing innovative immunotherapies and anti-tumor strategies for DLBCL.Methods The study utilized GEO data to identify prognostically significant genes via Cox regression and developed a Lasso-Cox-based prognostic model.Patients were divided into high-and low-risk groups,with survival differences analyzed using Kaplan-Meier.Independent prognostic factors were identified through univariate and multivariate Cox analyses,and model accuracy was evaluated with ROC curves.A nomogram was developed utilizing the"rms"R package.Immune infiltration and microenvironment differences were assessed with CIBERSORT,ssGSEA,and ESTIMATE.Results Our study developed a prognostic model using 10 genes that effectively categorized DLBCL patients into high-and low-risk cohorts,and the high-risk group revealed poorer outcomes(P<0.001).Predictive AUCs for survival at 1,3,and 5 years were 0.667,0.727,and 0.729.Significant differences were observed in DLBCL samples stratified by stage,ECOG score,and LDH level.In patients aged<65 years,with<2 extranodal sites,ECOG<2,GCB or non-GCB subtype,normal LDH levels,or stage III-IV,high-risk patients had worse survival outcomes(P<0.05).The risk score model outperformed other clinicopathological factors in predicting OS(P<0.0001,HR=3.373)and was an independent risk factor alongside age,ECOG score,and COO classification.Significant differences were observed in immune cell infiltration,immune functions,checkpoints,and tumor priming between risk groups.Conclusions In this study,we developed a risk scoring model based on endoplasmic reticulum stress and immunity to stratify DLBCL patients into low-and high-risk groups.Integrating this prognostic model with clinical parameters,we constructed a comprehensive nomogram.The identification of 10 key genes offers valuable prognostic targets for treatment and provides new perspectives for advancing therapeutic strategies in DLBCL.展开更多
Developing cost-effective and high-performance oxygen evolution reaction(OER)electrocatalysts has become the intense research on pursuing emerging renewable energy conversion,in which exploring and investigating the i...Developing cost-effective and high-performance oxygen evolution reaction(OER)electrocatalysts has become the intense research on pursuing emerging renewable energy conversion,in which exploring and investigating the intrinsic nature of efficient and stable Cu Co spinel catalysts toward OER in alkaline media is highly desirable.Herein,Cu1–xCo2+xO4oxy-spinel nanoflakes are fabricated by a facile hydrothermal method with the oxidation of ammonia water.In the same condition,Cu1–xCo2+xS4thio-spinel nanospheres are formed without oxidation.In OER process,the as-obtained Cu1–xCo2+xO4nanoflakes and Cu1–xCo2+xS4nanospheres possess the anodic overpotential of 267 and 297 m V in alkaline media to drive the current density of 10 m A/cm^2,respectively,outperforming the state-of-the-art noble metal catalyst of RuO2.X-ray photoelectron spectroscopy analysis exhibits the higher ratio value of Co(Ⅲ)/Co(Ⅱ)in Cu1–xCo2+xO4than that in Cu1–xCo2+xS4,suggesting that the stronglyelectronegative oxygen efficiently predominates in regulating valence states of Co active sites in spinel structures.Remarkably,density functional theory simulation further reveals that the increased valence state of Co could accelerate the electron exchange between catalysts and oxygen adsorbates during electrocatalysis,thus contributing to the higher OER activity of Cu1–xCo2+xO4catalysts.This work provides deep insight regarding the significance of non-metal element(O and S)in Cu Co spinel structure catalysts,as well as presents a promising approach to exploit higher performance and grasp the mechanism of various non-noblemetal spinel catalysts for water oxidation.展开更多
Background With the aggregation of clinical data and the evolution of computational resources,artificial intelligence-based methods have become possible to facilitate clinical diagnosis.For congenital heart disease(CH...Background With the aggregation of clinical data and the evolution of computational resources,artificial intelligence-based methods have become possible to facilitate clinical diagnosis.For congenital heart disease(CHD)detection,recent deep learning-based methods tend to achieve classification with few views or even a single view.Due to the complexity of CHD,the input images for the deep learning model should cover as many anatomical structures of the heart as possible to enhance the accuracy and robustness of the algorithm.In this paper,we first propose a deep learning method based on seven views for CHD classification and then validate it with clinical data,the results of which show the competitiveness of our approach.Methods A total of 1411 children admitted to the Children’s Hospital of Zhejiang University School of Medicine were selected,and their echocardiographic videos were obtained.Then,seven standard views were selected from each video,which were used as the input to the deep learning model to obtain the final result after training,validation and testing.Results In the test set,when a reasonable type of image was input,the area under the curve(AUC)value could reach 0.91,and the accuracy could reach 92.3%.During the experiment,shear transformation was used as interference to test the infection resistance of our method.As long as appropriate data were input,the above experimental results would not fluctuate obviously even if artificial interference was applied.Conclusions These results indicate that the deep learning model based on the seven standard echocardiographic views can effectively detect CHD in children,and this approach has considerable value in practical application.展开更多
Background High-dose cytarabine(HDAC)is commonly used for consolidation therapy in young acute myeloid leukemia(AML)patients,but the dosage of cytarabine is still controversial in the clinic due to its obvious post-ch...Background High-dose cytarabine(HDAC)is commonly used for consolidation therapy in young acute myeloid leukemia(AML)patients,but the dosage of cytarabine is still controversial in the clinic due to its obvious post-chem-otherapy adverse effects.The aim of this study was to contrast the efficacy in different dose groups of cytarabine after consolidation therapy in Chinese AML patients.Methods AML patients treated with cytarabine consolidation at Qilu Hospital,Shandong University from January 2010 to September 2022 were retrospectively analyzed,from which 346 AML patients with relatively complete follow-up data were selected for this study.We compared the patients’overall survival(OS)rate,relapse-free survival(RFS)rate,and hematologic adverse events in terms of their general characteristics,cytarabine consolidation therapy dose,consolidation course,2022 European Leukemia Net(ELN)risk stratification,and transplantation.Results In AML patients under 60 years of age,the 5-year RFS rate with high-dose cytarabine consolidation therapy was superior to that of small-dose cytarabine(P=0.024),while the 5-year RFS rate was comparable in the high-dose and intermediate-dose groups,and there was no obvious difference among the three groups in the 5-year OS rate(P>0.05).OS and RFS of those given more than 3 courses of cytarabine consolidation therapy were better than those in the 1–2 courses group(P=0.060,P=0.040).OS and RFS were better in patients with cumulative dose of cytarabine≥36g than in patients with cumulative dose<36g(P<0.05),but cumulative dose≥54g was comparable in OS and RFS with≥36–<54g group(P>0.05).There was no significant difference in hematologic adverse effects among the three treatment groups.In the latest ELN risk stratification favorable-risk group,the cumulative dose of cytarabine≥36g had a better 5-year RFS rate than the<36g group(P=0.038),and in the intermediate-risk group the 5-year OS rate and RFS rate were better in the≥36g group than the<36g group(P=0.012,0.025).In addition,the prognosis of transplanted patients was better than that of non-transplanted patients,whereas in non-transplanted patients,consolidation therapy with≥36g cytarabine can effectively improve outcomes.Multivariate analysis indicated that age,fibrinogen(FIB)and the cumulative dose of cytarabine of≥36–<54g were predictors of OS,while age,white blood cell(WBC)and HDAC were predictors of RFS.Conclusion The results of the study showed that consolidation therapy with cytarabine up to a cumulative dose of≥36–<54g in AML patients who did not undergo transplantation significantly improved patient prognosis.In the latest ELN risk stratification,cumulative doses of cytarabine≥36g had a better prognosis in favorable and interme-diate-risk patients.展开更多
Purpose The aim of this study was to explore the relationships between single-nucleotide polymorphisms(SNPs)of crucial molecules in the cGAS-STING signalling pathway and the susceptibility to,induction chemotherapy re...Purpose The aim of this study was to explore the relationships between single-nucleotide polymorphisms(SNPs)of crucial molecules in the cGAS-STING signalling pathway and the susceptibility to,induction chemotherapy response of,and prognosis of acute myeloid leukaemia(AML)in Chinese patients.Methods Thirteen SNPs of crucial molecules in the cGAS-STING signalling pathway were genotyped in 262 AML patients using the Sequenom MassARRAY system.The associations of SNPs with susceptibility,and induction chemo-therapy response were analysed using the chi-square test or Fisher’s exact test and univariate binary logistic regres-sion,the connection of SNPs with prognosis of AML was analysed using the log-rank test,and Kaplan–Meier curves were applied for survival estimation.Results In our study,gene polymorphisms of cGAS-STING signalling pathway molecules could be vitally associated with AML.In the recessive model,the cGAS rs311678 gene polymorphism could be closely related to AML suscep-tibility(CC vs.TT+TC,odds ratio(OR)=0.480,95%confidence interval(CI)=0.260–0.889,p=0.020).Moreover,IKKA rs3808917 might be associated with the WBC count,cGAS rs311678 could be associated with the bone marrow(BM)blast percentage,and NF-κB rs1056890 under codominant and recessive models could be connected with the HGB level.Patients who were STING rs7380272 TT/CT carriers was likely to have higher insensitivity to induction chemo-therapy than CC carriers(TT+CT vs.CC,OR=2.917,95%CI=1.073–7.929,p=0.036).Survival analysis indicated that the IKKB rs3747811 TT genotype might be associated with decreased overall survival(OS)(p<0.05).Conclusions SNPs of molecules in the cGAS-STING signalling pathway could be significantly associated with AML.The cGAS rs311678 gene polymorphism could be associated with AML susceptibility,the STING rs7380272 variant might be related to induction chemotherapy response,and IKKB rs3747811 tended to be associated with AML overall survival.Moreover,IKKA rs3808917 could be associated with the WBC count,cGAS rs311678 could be associated with the BM blast percentage,and NF-κB rs1056890 might be related to the HGB level.展开更多
Correction:Holist Integ Oncol 3,15(2024)https://doi.org/10.1007/s44178-024-00079-8 Following publication of the original article[1],the authors reported errors in Table 2,which have been corrected from.
基金supported by the National Natural Science Foundation of China(82102315).
文摘BACKGROUND:The lack of a stable,easy-to-operate animal model for severe trauma has hindered the research progress.The aim of this study is to develop a mouse model that replicates the pathophysiological conditions of severe trauma,providing a reliable research tool.METHODS:Male C57BL/6J mice(aged 8-10 weeks and weighting approximately 20 g)were used to establish the severe trauma model.Under anesthesia,a midshaft femoral fracture was created and packed with sterile cotton.A midline incision was made from the inguinal region to the sternum,exposing the abdominal organs for 30 min.The right femoral artery was cannulated to induce controlled blood loss at 30%,35%,40%,and 50%of the total blood volume.Survival rates were monitored for 24 h post-induction.In the mice that experienced 30%blood loss,the mean arterial pressure,body temperature,blood gas parameters,peripheral blood inflammatory markers,and major organ pathological changes were assessed.RESULTS:Mice with femoral fractures,soft tissue injuries,abdominal organ exposure,and 30%blood loss exhibited stable survival rates.Increased blood loss significantly reduced survival rates.Mean arterial pressure decreased initially,recovering within 0-15 min and returning to baseline by 50 min.Similarly,the body temperature decreased initially and gradually recovered to baseline within 50 min.Levels of peripheral blood inflammatory markers remained elevated for 12 h post-injury.Distant organs,including intestines,lungs,liver,spleen and kidneys,displayed varying degrees of injury.CONCLUSION:The established mouse model replicates the pathophysiological responses to severe trauma,indicating stability and reproducibility,which could be an useful tool for further trauma research.
基金supported by the National Natural Sci-ence Foundation of China(81901519)Anhui Provincial Natural Science Foundation(1908085QH380)and the Talent Scientific Research Start-up Foundation of Yijis-han Hospital,Wannan Medical College(YR201901).
文摘Objective Many studies have revealed the crucial roles of miRNA in multiple human cancers,including lung adenocarcinoma(LUAD).In this study,we sought to explore new miRNA-mRNA pairs that are associated with LUAD prognosis.Methods A novel miRNA-mRNA regulatory network associated with prognosis in LUAD was identified and validated using the bioinformatic tools including OncomiR database,StarBase,miRnet,GEPIA2,UALCAN.Results Twenty key miRNAs were compiled after the analysis of the expression and prognostic value in OncomiR and StarBase.Targeted mRNAs of these key miRNAs were predicted in miRnet,and the resulting mRNAs were also analyzed for their prognostic values and expression patterns in GEPIA2 and UALCAN,respectively.Further expression correlation analysis was performed in StarBase.Subsequently,a new miRNA-mRNA network was built,of which each RNA pair showed negative expression correlation,opposite expression pattern,and prognostic value.Protein-protein interaction network was under construction for the mRNAs,and 19 hub genes were determined.Enrichment analysis showed that“Cell Cycle,Mitotic”was the most significantly enriched term.Then,a miRNA-hub gene sub-network was built.We selected and validated the regulatory relationship of some miRNA-hub pairs,including hsa-miR-1976/RFC2,hsa-let-7c-5p/RFC2,hsa-let-7c-5p/ESPL1,hsa-let-7c-5p/CDC25A,and hsa-miR-101-3p/KIF2C.Moreover,over-expression of hsa-miR-1976 and hsa-let-7c-5p resulted in significant cell cycle arrest.Conclusions Our results determined new prognosis-associated miRNA-mRNA pairs and might shed further light on the mechanism via which miRNA-mRNA network influences prognosis in LUAD.
基金Jiangxi Educational Science 13th Five-Year Plan Planning Project(20YB003)2022 Nanchang University Aesthetic Education Special Project(MY2217)。
文摘Costume is not only an important carrier of human civilization,but also reflects the development level of material and spiritual civilization.In modern costume design,we have incorporated colorful cultural elements,which are not only the embodiment of aesthetics but also the aesthetic expression based on culture.Printmaking art,as a kind of traditional art,has its unique style and characteristics.In teaching practice,we instruct students to integrate the creative spirit and formal elements of printmaking into costume design,to realize the inheritance and innovation of traditional culture.Under the background of globalization,we should be more committed to inheriting and carrying forward the excellent Chinese national culture and promoting the popularization and dissemination of traditional culture.Exploring the application of printmaking art in costume design through teaching practice aims to construct a new teaching mode,which provides a reference for the application of other traditional arts in modern design teaching.
基金the Shandong Provincial Natural Science Foundation(ZR2020MH119,ZR2020MH118)Distinguished Taishan Scholars in Climbing Plan(tspd20210321)+1 种基金National Nature Science Foundation of China(81400118,81700143)the horizontal subject of Shandong University(6010120072).
文摘A middle-aged man was diagnosed with myelodysplastic/myeloproliferative neoplasm-unclassifiable(MDS/MPN-U),with severe and extensive corticosteroid-resistant Sweet’s syndrome(SS)as the first manifestation,with evidence of clinical manifestations,pathological description,and laboratory evaluation.The skin lesions continued to spread despite treatment with systemic corticosteroids but were successfully treated with intravenous immunoglobulin(IVIG).
基金supported by grants from the Distinguished Taishan Scholars in Climbing Plan(tspd20210321)the Distinguished Taishan Scholars Plan(tstp20230653)the National Natural Science Foundation of China(82170182,82470153,82470180)+2 种基金the horizontal subject of Shandong University(6010123101)the Young Elite sponsorship Program of Shandong Provincial Medical Association(2023-LC-0336)the Innovative Team of Outstanding Youth in Colleges and Universities of Shandong Province(2023KJ022).
文摘Purpose The study aimed to integrate genes associated with endoplasmic reticulum stress and immune responses to create a validated prognostic model for DLBCL.The research identified novel prognostic model and provided insights for developing innovative immunotherapies and anti-tumor strategies for DLBCL.Methods The study utilized GEO data to identify prognostically significant genes via Cox regression and developed a Lasso-Cox-based prognostic model.Patients were divided into high-and low-risk groups,with survival differences analyzed using Kaplan-Meier.Independent prognostic factors were identified through univariate and multivariate Cox analyses,and model accuracy was evaluated with ROC curves.A nomogram was developed utilizing the"rms"R package.Immune infiltration and microenvironment differences were assessed with CIBERSORT,ssGSEA,and ESTIMATE.Results Our study developed a prognostic model using 10 genes that effectively categorized DLBCL patients into high-and low-risk cohorts,and the high-risk group revealed poorer outcomes(P<0.001).Predictive AUCs for survival at 1,3,and 5 years were 0.667,0.727,and 0.729.Significant differences were observed in DLBCL samples stratified by stage,ECOG score,and LDH level.In patients aged<65 years,with<2 extranodal sites,ECOG<2,GCB or non-GCB subtype,normal LDH levels,or stage III-IV,high-risk patients had worse survival outcomes(P<0.05).The risk score model outperformed other clinicopathological factors in predicting OS(P<0.0001,HR=3.373)and was an independent risk factor alongside age,ECOG score,and COO classification.Significant differences were observed in immune cell infiltration,immune functions,checkpoints,and tumor priming between risk groups.Conclusions In this study,we developed a risk scoring model based on endoplasmic reticulum stress and immunity to stratify DLBCL patients into low-and high-risk groups.Integrating this prognostic model with clinical parameters,we constructed a comprehensive nomogram.The identification of 10 key genes offers valuable prognostic targets for treatment and provides new perspectives for advancing therapeutic strategies in DLBCL.
基金the support from the National Natural Science Foundation of China(91750112,51801075)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX191591).D Rao gratefully acknowledges the support of Jiangsu Overseas Visiting Scholar Program for University Prominent Young and Mid-aged Teachers and Presidents.
文摘Developing cost-effective and high-performance oxygen evolution reaction(OER)electrocatalysts has become the intense research on pursuing emerging renewable energy conversion,in which exploring and investigating the intrinsic nature of efficient and stable Cu Co spinel catalysts toward OER in alkaline media is highly desirable.Herein,Cu1–xCo2+xO4oxy-spinel nanoflakes are fabricated by a facile hydrothermal method with the oxidation of ammonia water.In the same condition,Cu1–xCo2+xS4thio-spinel nanospheres are formed without oxidation.In OER process,the as-obtained Cu1–xCo2+xO4nanoflakes and Cu1–xCo2+xS4nanospheres possess the anodic overpotential of 267 and 297 m V in alkaline media to drive the current density of 10 m A/cm^2,respectively,outperforming the state-of-the-art noble metal catalyst of RuO2.X-ray photoelectron spectroscopy analysis exhibits the higher ratio value of Co(Ⅲ)/Co(Ⅱ)in Cu1–xCo2+xO4than that in Cu1–xCo2+xS4,suggesting that the stronglyelectronegative oxygen efficiently predominates in regulating valence states of Co active sites in spinel structures.Remarkably,density functional theory simulation further reveals that the increased valence state of Co could accelerate the electron exchange between catalysts and oxygen adsorbates during electrocatalysis,thus contributing to the higher OER activity of Cu1–xCo2+xO4catalysts.This work provides deep insight regarding the significance of non-metal element(O and S)in Cu Co spinel structure catalysts,as well as presents a promising approach to exploit higher performance and grasp the mechanism of various non-noblemetal spinel catalysts for water oxidation.
基金the Public Welfare Technology Application Research Project of Zhejiang Science and Technology Department(LGF22H180002)Key Research and Development Plan of Zhejiang Province(No.2022C03087).
文摘Background With the aggregation of clinical data and the evolution of computational resources,artificial intelligence-based methods have become possible to facilitate clinical diagnosis.For congenital heart disease(CHD)detection,recent deep learning-based methods tend to achieve classification with few views or even a single view.Due to the complexity of CHD,the input images for the deep learning model should cover as many anatomical structures of the heart as possible to enhance the accuracy and robustness of the algorithm.In this paper,we first propose a deep learning method based on seven views for CHD classification and then validate it with clinical data,the results of which show the competitiveness of our approach.Methods A total of 1411 children admitted to the Children’s Hospital of Zhejiang University School of Medicine were selected,and their echocardiographic videos were obtained.Then,seven standard views were selected from each video,which were used as the input to the deep learning model to obtain the final result after training,validation and testing.Results In the test set,when a reasonable type of image was input,the area under the curve(AUC)value could reach 0.91,and the accuracy could reach 92.3%.During the experiment,shear transformation was used as interference to test the infection resistance of our method.As long as appropriate data were input,the above experimental results would not fluctuate obviously even if artificial interference was applied.Conclusions These results indicate that the deep learning model based on the seven standard echocardiographic views can effectively detect CHD in children,and this approach has considerable value in practical application.
文摘Background High-dose cytarabine(HDAC)is commonly used for consolidation therapy in young acute myeloid leukemia(AML)patients,but the dosage of cytarabine is still controversial in the clinic due to its obvious post-chem-otherapy adverse effects.The aim of this study was to contrast the efficacy in different dose groups of cytarabine after consolidation therapy in Chinese AML patients.Methods AML patients treated with cytarabine consolidation at Qilu Hospital,Shandong University from January 2010 to September 2022 were retrospectively analyzed,from which 346 AML patients with relatively complete follow-up data were selected for this study.We compared the patients’overall survival(OS)rate,relapse-free survival(RFS)rate,and hematologic adverse events in terms of their general characteristics,cytarabine consolidation therapy dose,consolidation course,2022 European Leukemia Net(ELN)risk stratification,and transplantation.Results In AML patients under 60 years of age,the 5-year RFS rate with high-dose cytarabine consolidation therapy was superior to that of small-dose cytarabine(P=0.024),while the 5-year RFS rate was comparable in the high-dose and intermediate-dose groups,and there was no obvious difference among the three groups in the 5-year OS rate(P>0.05).OS and RFS of those given more than 3 courses of cytarabine consolidation therapy were better than those in the 1–2 courses group(P=0.060,P=0.040).OS and RFS were better in patients with cumulative dose of cytarabine≥36g than in patients with cumulative dose<36g(P<0.05),but cumulative dose≥54g was comparable in OS and RFS with≥36–<54g group(P>0.05).There was no significant difference in hematologic adverse effects among the three treatment groups.In the latest ELN risk stratification favorable-risk group,the cumulative dose of cytarabine≥36g had a better 5-year RFS rate than the<36g group(P=0.038),and in the intermediate-risk group the 5-year OS rate and RFS rate were better in the≥36g group than the<36g group(P=0.012,0.025).In addition,the prognosis of transplanted patients was better than that of non-transplanted patients,whereas in non-transplanted patients,consolidation therapy with≥36g cytarabine can effectively improve outcomes.Multivariate analysis indicated that age,fibrinogen(FIB)and the cumulative dose of cytarabine of≥36–<54g were predictors of OS,while age,white blood cell(WBC)and HDAC were predictors of RFS.Conclusion The results of the study showed that consolidation therapy with cytarabine up to a cumulative dose of≥36–<54g in AML patients who did not undergo transplantation significantly improved patient prognosis.In the latest ELN risk stratification,cumulative doses of cytarabine≥36g had a better prognosis in favorable and interme-diate-risk patients.
基金supported by grants from the Distinguished Taishan Scholars in Climbing Plan(tspd20210321)Taishan Scholars-Distinguished Experts(tstp20230653)+3 种基金the National Natural Science Foundation of China(8200016582370165)the Key Program of Natural Science Foundation of Shandong Province(ZR2021MH302)the Horizontal Subject of Shandong University(6010121004).
文摘Purpose The aim of this study was to explore the relationships between single-nucleotide polymorphisms(SNPs)of crucial molecules in the cGAS-STING signalling pathway and the susceptibility to,induction chemotherapy response of,and prognosis of acute myeloid leukaemia(AML)in Chinese patients.Methods Thirteen SNPs of crucial molecules in the cGAS-STING signalling pathway were genotyped in 262 AML patients using the Sequenom MassARRAY system.The associations of SNPs with susceptibility,and induction chemo-therapy response were analysed using the chi-square test or Fisher’s exact test and univariate binary logistic regres-sion,the connection of SNPs with prognosis of AML was analysed using the log-rank test,and Kaplan–Meier curves were applied for survival estimation.Results In our study,gene polymorphisms of cGAS-STING signalling pathway molecules could be vitally associated with AML.In the recessive model,the cGAS rs311678 gene polymorphism could be closely related to AML suscep-tibility(CC vs.TT+TC,odds ratio(OR)=0.480,95%confidence interval(CI)=0.260–0.889,p=0.020).Moreover,IKKA rs3808917 might be associated with the WBC count,cGAS rs311678 could be associated with the bone marrow(BM)blast percentage,and NF-κB rs1056890 under codominant and recessive models could be connected with the HGB level.Patients who were STING rs7380272 TT/CT carriers was likely to have higher insensitivity to induction chemo-therapy than CC carriers(TT+CT vs.CC,OR=2.917,95%CI=1.073–7.929,p=0.036).Survival analysis indicated that the IKKB rs3747811 TT genotype might be associated with decreased overall survival(OS)(p<0.05).Conclusions SNPs of molecules in the cGAS-STING signalling pathway could be significantly associated with AML.The cGAS rs311678 gene polymorphism could be associated with AML susceptibility,the STING rs7380272 variant might be related to induction chemotherapy response,and IKKB rs3747811 tended to be associated with AML overall survival.Moreover,IKKA rs3808917 could be associated with the WBC count,cGAS rs311678 could be associated with the BM blast percentage,and NF-κB rs1056890 might be related to the HGB level.
文摘Correction:Holist Integ Oncol 3,15(2024)https://doi.org/10.1007/s44178-024-00079-8 Following publication of the original article[1],the authors reported errors in Table 2,which have been corrected from.
