Isolated micmspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of aetivatod carbon on embryogenesis, and...Isolated micmspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of aetivatod carbon on embryogenesis, and the concentration of NAA on induction of roots. The results showed that the embryogenesis on medi- um subjected to filter sterilization is better than medium subjected to autoclaved sterilization. The embryos cultured for 15 d had the highest plantlat regenexafion rate （52%）. NLN-13 liquid medium including 0. 1mg/L 6-BA and 0.2 g/L activated carbon significantly improved the planflet regeneration rate. 1/2 MS inclu- ding 0.3 mg/L NAA had the highest plantlet regeneration rate （ 100% ）. Key words Brassica juncea ; Isolated micmspore culture ; Embryogenesis ; Planflet regeneration展开更多
In this study, using Japanese radish cytoplasmic sterile line ＂Half Green Cabbage＂ （HGC） and clubfoot-resistant South Korean cabbage variety ＂ CR Cabbage King＂ （CCK） as experimental materials, serf-incompatibl...In this study, using Japanese radish cytoplasmic sterile line ＂Half Green Cabbage＂ （HGC） and clubfoot-resistant South Korean cabbage variety ＂ CR Cabbage King＂ （CCK） as experimental materials, serf-incompatible DH lines were obtained by mierosporo culture. Through five gnerations of backeross, CCR11239, a clubroot-resistant radish cytoplasmic sterile line, was obtained. Through six generations of serf-crossing, CCR11240, the maintainer line of CCR11239, was bred. After the cross of CCK and Luchunbai 1 （83 - 1 ） and five generations of self-crossing, serf-compatible line CCRl1241 was obtained. Through crossing CCR11240 with CCRl1241, the new cabbage variety CCRl1242 was obtained. After variety comparison test, provincial regional test and production demonstration test, the new variety was registered in 2012. The average yield of CCR11242 reached 60 975 kg/hm2, which was improved by 142% compared with Luchunbai 1 （83 - 1 ）. The disease index of CCR11242 was 5.63, which was 88.13 lower than the control, indicating high resistance （HR） to clubfoot.展开更多
Clubroot of Chinese cabbage(Brassica rapa L.ssp.pekinensis),caused by the obligate parasite Plasmodiophora brassicae,accounts for serious yield losses.The aim of our study was to explore the phytohormone levels and me...Clubroot of Chinese cabbage(Brassica rapa L.ssp.pekinensis),caused by the obligate parasite Plasmodiophora brassicae,accounts for serious yield losses.The aim of our study was to explore the phytohormone levels and metabolome changes in the roots of resistant and susceptible B.rapa genotypes at a late stage of infection,i.e.,28 days post-infection.Both genotypes showed decreased auxin levels after P.brassicae infection except for indole-3-acetic acid.Overall,the susceptible genotype had higher auxin and cytokinin levels after infection,with the exception of trans-zeatin and 3-indolebutyric acid as compared to the resistant genotype.Jasmonic acid levels declined after infection regardless of the genotype.Resistance against clubroot was evident with the increased levels of salicylic acid in the resistant genotype.The susceptible genotype had a higher number of differentially accumulated metabolites(DAMs)(262)than the resistant genotype(238)after infection.Interestingly,132 DAMs were commonly detected in both genotypes when infected with the pathogen,belonging to metabolite classes such as phenolic acids,amino acids,and derivatives,glucosinolates,organic acids,flavonoids,nucleotides and derivatives,and fatty acids.The differential metabolite analysis revealed that metabolites related to amino acid biosynthesis,fatty acid biosynthesis and elongation,glutathione metabolism,and glucosinolate metabolism were highly accumulated in the resistant genotype,suggesting their essential roles in resistance against P.brassicae infection.展开更多
Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the fu...Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the future. Genomic DNA was isolated from Chinese cabbage by CTAB method. The design of two pairs of specific primers was performed on conserved flanking region of orf138 gene in the GenBank. PCR was performed with genomic DNA of the nine Chinese cabbage materials. The bands were sequenced. The homologous comparison was conducted in NCBI, and finally, the type of sterile cytoplasm was determined. The results showed that the bands were amplified only in four Chinese cabbage male sterile materials with two pairs of specific primers PUPIl and PIII/PIV, while the other five materials did not obtain the relative bands. The result was consistent with the field sterility identification. And then four molecular markers of Chinese cabbage Ogura cytoplasmic male sterility （CMS） were obtained. After conducting a homologous comparative analysis with BLAST in GenBank, it was found that the homologous degree was 100% in specific segments of tbe tbree sterility materials （L1-CI, L3-CI and L3- F1 ） and Ogu orf138 gene （GenBank accession No. ： HQ149728） of the reported broccoli Ogu CMS. The homologous degree of L1-F1 was 99% with a variation point. The type of cytoplasmic male sterility of the other five materials needed further research. Four materials of the nine were identified as the radish cytoplasmic male sterility materials and four molecular markers were obtained.展开更多
基金Supported by Key New Product Project of Science and Technology Department of Yunnan Province(2015BB007,2012BB017)International Cooperation Program of Science and Technology Department of Yunnan Province(2014IA016)+2 种基金Science and Technology Specific Project for Enriching People and Strengthening County Economy of Science and Technology Department of Yunnan Province(2014EB033)National Large Vegetable Industry Technology System(CARS-25-G-45)New Vegetable Variety Collaborative Project of Agriculture Department of Yunnan Province[YCN(2012)58]
文摘Isolated micmspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of aetivatod carbon on embryogenesis, and the concentration of NAA on induction of roots. The results showed that the embryogenesis on medi- um subjected to filter sterilization is better than medium subjected to autoclaved sterilization. The embryos cultured for 15 d had the highest plantlat regenexafion rate （52%）. NLN-13 liquid medium including 0. 1mg/L 6-BA and 0.2 g/L activated carbon significantly improved the planflet regeneration rate. 1/2 MS inclu- ding 0.3 mg/L NAA had the highest plantlet regeneration rate （ 100% ）. Key words Brassica juncea ; Isolated micmspore culture ; Embryogenesis ; Planflet regeneration
基金Supported by Major Project of New Product Development of Yunnan Provincial Science and Technology Department(2015BB007,2012BB017)International Cooperation Project of Yunnan Provincial Science and Technology Department(2014IA016)+2 种基金Project of Science and Technology to Strengthen the County and Enrich the People of Yunnan Provincial Science and Technology Department(2014EB033)National Bulk Vegetable Industry Technology System of China(CARS-25-G-45)New Vegetable Variety Cooperation Project of Yunnan Provincial Department of Agriculture[YCN(2012)No.58]
文摘In this study, using Japanese radish cytoplasmic sterile line ＂Half Green Cabbage＂ （HGC） and clubfoot-resistant South Korean cabbage variety ＂ CR Cabbage King＂ （CCK） as experimental materials, serf-incompatible DH lines were obtained by mierosporo culture. Through five gnerations of backeross, CCR11239, a clubroot-resistant radish cytoplasmic sterile line, was obtained. Through six generations of serf-crossing, CCR11240, the maintainer line of CCR11239, was bred. After the cross of CCK and Luchunbai 1 （83 - 1 ） and five generations of self-crossing, serf-compatible line CCRl1241 was obtained. Through crossing CCR11240 with CCRl1241, the new cabbage variety CCRl1242 was obtained. After variety comparison test, provincial regional test and production demonstration test, the new variety was registered in 2012. The average yield of CCR11242 reached 60 975 kg/hm2, which was improved by 142% compared with Luchunbai 1 （83 - 1 ）. The disease index of CCR11242 was 5.63, which was 88.13 lower than the control, indicating high resistance （HR） to clubfoot.
基金the National Bulk Vegetable Industry Technology System of China(CARS-23-G37)Research and integrated demonstration of green key technologies of main export vegetables in Yunnan Province(2019ZG001)Yunnan Province“ten thousand talents plan”Yunling industrial technology leading talents,Special support of Modern Agricultural Technology Department of vegetables in Yunnan Province.The funder has no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Clubroot of Chinese cabbage(Brassica rapa L.ssp.pekinensis),caused by the obligate parasite Plasmodiophora brassicae,accounts for serious yield losses.The aim of our study was to explore the phytohormone levels and metabolome changes in the roots of resistant and susceptible B.rapa genotypes at a late stage of infection,i.e.,28 days post-infection.Both genotypes showed decreased auxin levels after P.brassicae infection except for indole-3-acetic acid.Overall,the susceptible genotype had higher auxin and cytokinin levels after infection,with the exception of trans-zeatin and 3-indolebutyric acid as compared to the resistant genotype.Jasmonic acid levels declined after infection regardless of the genotype.Resistance against clubroot was evident with the increased levels of salicylic acid in the resistant genotype.The susceptible genotype had a higher number of differentially accumulated metabolites(DAMs)(262)than the resistant genotype(238)after infection.Interestingly,132 DAMs were commonly detected in both genotypes when infected with the pathogen,belonging to metabolite classes such as phenolic acids,amino acids,and derivatives,glucosinolates,organic acids,flavonoids,nucleotides and derivatives,and fatty acids.The differential metabolite analysis revealed that metabolites related to amino acid biosynthesis,fatty acid biosynthesis and elongation,glutathione metabolism,and glucosinolate metabolism were highly accumulated in the resistant genotype,suggesting their essential roles in resistance against P.brassicae infection.
基金Supported by National Staple Vegetable Industrial Technology System(CARS-23-G37)Breeding and Industrialization Demonstration of New Varieties of Cruciferae Vegetables(Brassica pekinensis Rupr.,Raphanus sativus L.,Brassica oleracea L.)in Yunnan Province(2015BB007)Basic Research Project of Yunnan Science and Technology Plan(2017FD200)
文摘Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the future. Genomic DNA was isolated from Chinese cabbage by CTAB method. The design of two pairs of specific primers was performed on conserved flanking region of orf138 gene in the GenBank. PCR was performed with genomic DNA of the nine Chinese cabbage materials. The bands were sequenced. The homologous comparison was conducted in NCBI, and finally, the type of sterile cytoplasm was determined. The results showed that the bands were amplified only in four Chinese cabbage male sterile materials with two pairs of specific primers PUPIl and PIII/PIV, while the other five materials did not obtain the relative bands. The result was consistent with the field sterility identification. And then four molecular markers of Chinese cabbage Ogura cytoplasmic male sterility （CMS） were obtained. After conducting a homologous comparative analysis with BLAST in GenBank, it was found that the homologous degree was 100% in specific segments of tbe tbree sterility materials （L1-CI, L3-CI and L3- F1 ） and Ogu orf138 gene （GenBank accession No. ： HQ149728） of the reported broccoli Ogu CMS. The homologous degree of L1-F1 was 99% with a variation point. The type of cytoplasmic male sterility of the other five materials needed further research. Four materials of the nine were identified as the radish cytoplasmic male sterility materials and four molecular markers were obtained.
