BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA prog...BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA progression through the ERK1/2 signaling cascade.AIM To determine the mechanism of the IL-22/IL-22R1 pathway in CCA and provide new directions for its clinical treatment.METHODS IL-22R1 expression was assessed in human and rat CCA tissues utilizing immunohistochemical techniques,Western blot analysis,and quantitative reverse transcription PCR.The impact of IL-22 on CCA cells was assessed in vitro via tests for proliferation,migration,invasion,and apoptosis assays.The rat models of thioacetamide-induced CCA and subcutaneous xenografts in nude mice were used to assess the in vivo effects.ERK1/2 inhibitors were applied to elucidate the mechanistic role of the pathway.RESULTS IL-22R1 was overexpressed in CCA cell lines and tissues.IL-22 treatment increased the phosphorylation of ERK1/2,promoting tumor cell proliferation,migration,invasion,and resistance to apoptosis.ERK1/2 inhibition considerably reversed these effects both in vitro and in vivo.CONCLUSION The IL-22/IL-22R1 axis promotes CCA progression by activating ERK1/2 signaling.Targeting this pathway with ERK1/2 inhibitors offers potential therapeutic strategies for CCA.展开更多
The Tarim Basin has revealed numerous tight sandstone oil and gas reservoirs.The tidal fl at zone in the Shunbei area is currently in the detailed exploration stage,requiring a comprehensive description of the sand bo...The Tarim Basin has revealed numerous tight sandstone oil and gas reservoirs.The tidal fl at zone in the Shunbei area is currently in the detailed exploration stage,requiring a comprehensive description of the sand body distribution characteristics for rational exploration well deployment.However,using a single method for sand body prediction has yielded poor results.Seismic facies analysis can eff ectively predict the macro-development characteristics of sedimentary sand bodies but lacks the resolution to capture fine details.In contrast,single-well sedimentary facies analysis can describe detailed sand body development but struggles to reveal broader trends.Therefore,this study proposes a method that combines seismic facies analysis with single-well sedimentary microfacies analysis,using the lower section of the Kepingtage Formation in the Shunbei area as a case study.First,seismic facies were obtained through unsupervised vector quantization to control the macro-distribution characteristics of sand bodies,while principal component analysis(PCA)was applied to improve the depiction of fine sand body details from seismic attributes.Based on 3D seismic data,well-logging data,and geological interpretation results,a detailed structural interpretation was performed to establish a high-precision stratigraphic framework,thereby enhancing the accuracy of sand body prediction.Seismic facies analysis was then conducted to obtain the macro-distribution characteristics of the sand bodies.Subsequently,core data and logging curves from individual wells were used to clarify the vertical development characteristics of tidal channels and sandbars.Next,PCA was employed to select the seismic attributes most sensitive to sand bodies in diff erent sedimentary facies.Results indicate that RMS amplitude in the subtidal zone and instantaneous phase in the intertidal zone are the most sensitive to sand bodies.A comparative analysis of individual seismic attributes for sand body characterization revealed that facies-based delineation improved the accuracy of sand body identification,eff ectively capturing their contours and shapes.This method,which integrates seismic facies,single-well sedimentary microfacies,and machine learning techniques,enhances the precision of sand body characterization and off ers a novel approach to sand body prediction.展开更多
BACKGROUND Several recent studies have confirmed that intratumoural microorganisms can affect the occurrence and development of hepatocellular carcinoma(HCC);however,their role in tumor progression remains unclear.Hen...BACKGROUND Several recent studies have confirmed that intratumoural microorganisms can affect the occurrence and development of hepatocellular carcinoma(HCC);however,their role in tumor progression remains unclear.Hence,there is a need for further research on the role of intratumoural microorganisms in HCC.AIM To investigate the changes in intratumoural microorganisms in HCC and the effect of Propionibacterium on HCC progression.METHODS HCC and normal liver tissue specimens were subjected to fluorescence in situ hybridization(FISH).After performing 16S rRNA sequencing on HCC and peritumoral tissues to analyze the differences between the two groups.Propionibacterium was cocultured with HCC cells in vitro.Changes in cell proliferation and migration capacity were evaluated.The expression of NF-κB pathway related proteins in tumor cells was compared.The orthotopic liver implantation model and the subcutaneous xenograft model were constructed.liver tissues and subcutaneous tumors were collected 2 weeks later.RESULTS FISH demonstrated the presence of microorganisms in HCC and normal liver tissues.16S rRNA sequencing revealed an abundance of Lysobacter,Lachnospiraceae,Pseudomonas,and Lactobacillus in HCC tissues.The distribution and abundance of Propionibacterium showed differences between HCC and peritumoral tissues(P<0.05).In vitro studies demonstrated that Propionibacterium and its metabolite propionic acid(PA)inhibited the proliferation and migration of HCC cells(P<0.05).The expression of the proteins in NF-κB signaling pathway also decreased in HCC cells(P<0.05).CONCLUSION Microorganisms in HCC and normal liver tissues displayed significant disparities.The PA-producing bacterium Propionibacterium in HCC exerts an effect on the NF-κB pathway,thereby affecting the biological behavior of HCC.展开更多
基金National Natural Science Foundation of China,No.82372194.
文摘BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA progression through the ERK1/2 signaling cascade.AIM To determine the mechanism of the IL-22/IL-22R1 pathway in CCA and provide new directions for its clinical treatment.METHODS IL-22R1 expression was assessed in human and rat CCA tissues utilizing immunohistochemical techniques,Western blot analysis,and quantitative reverse transcription PCR.The impact of IL-22 on CCA cells was assessed in vitro via tests for proliferation,migration,invasion,and apoptosis assays.The rat models of thioacetamide-induced CCA and subcutaneous xenografts in nude mice were used to assess the in vivo effects.ERK1/2 inhibitors were applied to elucidate the mechanistic role of the pathway.RESULTS IL-22R1 was overexpressed in CCA cell lines and tissues.IL-22 treatment increased the phosphorylation of ERK1/2,promoting tumor cell proliferation,migration,invasion,and resistance to apoptosis.ERK1/2 inhibition considerably reversed these effects both in vitro and in vivo.CONCLUSION The IL-22/IL-22R1 axis promotes CCA progression by activating ERK1/2 signaling.Targeting this pathway with ERK1/2 inhibitors offers potential therapeutic strategies for CCA.
基金Collaborative Project Grant from the Exploration and Development Research Institute of SINOPEC Northwest Oilfi eld Company(Grant No.KY2021-S-104).
文摘The Tarim Basin has revealed numerous tight sandstone oil and gas reservoirs.The tidal fl at zone in the Shunbei area is currently in the detailed exploration stage,requiring a comprehensive description of the sand body distribution characteristics for rational exploration well deployment.However,using a single method for sand body prediction has yielded poor results.Seismic facies analysis can eff ectively predict the macro-development characteristics of sedimentary sand bodies but lacks the resolution to capture fine details.In contrast,single-well sedimentary facies analysis can describe detailed sand body development but struggles to reveal broader trends.Therefore,this study proposes a method that combines seismic facies analysis with single-well sedimentary microfacies analysis,using the lower section of the Kepingtage Formation in the Shunbei area as a case study.First,seismic facies were obtained through unsupervised vector quantization to control the macro-distribution characteristics of sand bodies,while principal component analysis(PCA)was applied to improve the depiction of fine sand body details from seismic attributes.Based on 3D seismic data,well-logging data,and geological interpretation results,a detailed structural interpretation was performed to establish a high-precision stratigraphic framework,thereby enhancing the accuracy of sand body prediction.Seismic facies analysis was then conducted to obtain the macro-distribution characteristics of the sand bodies.Subsequently,core data and logging curves from individual wells were used to clarify the vertical development characteristics of tidal channels and sandbars.Next,PCA was employed to select the seismic attributes most sensitive to sand bodies in diff erent sedimentary facies.Results indicate that RMS amplitude in the subtidal zone and instantaneous phase in the intertidal zone are the most sensitive to sand bodies.A comparative analysis of individual seismic attributes for sand body characterization revealed that facies-based delineation improved the accuracy of sand body identification,eff ectively capturing their contours and shapes.This method,which integrates seismic facies,single-well sedimentary microfacies,and machine learning techniques,enhances the precision of sand body characterization and off ers a novel approach to sand body prediction.
基金Supported by the Tianjin Health Science and Technology Project Surface Project,No.TJWJ2023MS012.
文摘BACKGROUND Several recent studies have confirmed that intratumoural microorganisms can affect the occurrence and development of hepatocellular carcinoma(HCC);however,their role in tumor progression remains unclear.Hence,there is a need for further research on the role of intratumoural microorganisms in HCC.AIM To investigate the changes in intratumoural microorganisms in HCC and the effect of Propionibacterium on HCC progression.METHODS HCC and normal liver tissue specimens were subjected to fluorescence in situ hybridization(FISH).After performing 16S rRNA sequencing on HCC and peritumoral tissues to analyze the differences between the two groups.Propionibacterium was cocultured with HCC cells in vitro.Changes in cell proliferation and migration capacity were evaluated.The expression of NF-κB pathway related proteins in tumor cells was compared.The orthotopic liver implantation model and the subcutaneous xenograft model were constructed.liver tissues and subcutaneous tumors were collected 2 weeks later.RESULTS FISH demonstrated the presence of microorganisms in HCC and normal liver tissues.16S rRNA sequencing revealed an abundance of Lysobacter,Lachnospiraceae,Pseudomonas,and Lactobacillus in HCC tissues.The distribution and abundance of Propionibacterium showed differences between HCC and peritumoral tissues(P<0.05).In vitro studies demonstrated that Propionibacterium and its metabolite propionic acid(PA)inhibited the proliferation and migration of HCC cells(P<0.05).The expression of the proteins in NF-κB signaling pathway also decreased in HCC cells(P<0.05).CONCLUSION Microorganisms in HCC and normal liver tissues displayed significant disparities.The PA-producing bacterium Propionibacterium in HCC exerts an effect on the NF-κB pathway,thereby affecting the biological behavior of HCC.
