Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabo...Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabolite genipin.This study explored the role of gut microbiota in alleviating geniposide hepatotoxicity with isoflavones in soy products.Metabolic profiling using ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q/TOF-MS)revealed two metabolic pathways and six main forms of geniposides in vivo.Enzyme inhibitor experiments have shown that isoflavones alter geniposide metabolism by mediating specific enzymes,includingβ-glucosidase(β-GC)and sulfotransferase(SULT),in an established pseudo-sterile rat model.Isoflavones pretreatment by gavage for three weeks optimized the structure of the gut microbiota was linked to the regulation of key metabolic enzymes.Furthermore,experiments involving fecal microbiota transplantation(FMT)established the direct contribution of the gut microbiota to the regulation of enzyme activities and geniposide metabolism.This study demonstrated that isoflavones in soy products regulated the metabolic enzymes of geniposode dependent on gut microbiota,especially Lactobacillus spp.,which was further verified in our clinical trials analyzed using 16S ribosomal RNA(rRNA)and metagenomic sequencing,thus regulating geniposide metabolism.Furthermore,as dominant beneficial bacterium,Lactobacillus spp.were discovered to be promising microbial targets for the better management of geniposide hepatotoxicity.These findings provide valuable insights for the prevention and intervention of drug-induced liver injury.展开更多
Although organic compounds are considered to be promising electrode materials with their remarkable characteristics such as diverse structures,design controllability,and environmental friendliness,their low charge-tra...Although organic compounds are considered to be promising electrode materials with their remarkable characteristics such as diverse structures,design controllability,and environmental friendliness,their low charge-transfer capability and limited cycling durability hinder their application in aqueous proton batteries.Herein,we prepared a noncovalent phenazine-based graphene aerogel(H/G)composite for aqueous proton storage,which is realized by redox-active Hexaazatrinaphthalene(HATN)organic compound combined with conductive reduced graphene oxide(rGO).The integration of rGO into HATN not only effectively optimizes the electronic structure of the H/G composite to enhance its electrochemical activity,but also the favorable noncovalent π-π interaction existed between HATN and rGO provides a stable structure for fast electron transportation.The obvious electron transfer in the aerogel composite promotes fast and reversible redox reactions occurred with the imino-active HATN in the composite electrode for proton uptake/removal in an aqueous acidic electrolyte,which are demonstrated by in-situ Fourier transform infrared(FTIR)investigation,theoretical calculations and experimental measurements.Therefore,it can deliver a fast,stable and efficient aqueous proton storage behavior with a large specific capacity of 274 mA·h·g^(-1) and considerable calendar life with~100%capacity retention after 3000 cycles,surpassing previously reported proton-based organic electrodes in aqueous acidic electrolytes.Furthermore,an outstanding soft-package aqueous proton(APB)has been fabricated with considerable long-term cycling stability.展开更多
Carbon monoxide(CO)oxidation is crucial for pollutant removal and hydrogen purification.In recent years,copper–cerium(Cu–Ce)-mixed oxide catalysts have attracted significant attention due to their excellent activity a...Carbon monoxide(CO)oxidation is crucial for pollutant removal and hydrogen purification.In recent years,copper–cerium(Cu–Ce)-mixed oxide catalysts have attracted significant attention due to their excellent activity and stability in CO oxida-tion.This study presents an innovative,environmentally friendly electrosynthesis method for producing stable,structured Cu–Ce catalysts in mesh form.This approach addresses the limitations of traditional pellet catalysts,such as fragility and poor thermal conductivity.The results demonstrated that incorporating cerium(Ce)enhanced the catalytic activity for CO oxidation threefold.A series of in situ characterizations revealed that the introduction of Ce led to the formation of a Cu–Ce mixed oxide solid solution,which significantly improved catalytic performance.Furthermore,higher pretreatment tem-peratures facilitated the decomposition of Ce compounds(nitrate and hydroxide),which promotes the formation of Cu–Ce solid solutions and increases the concentration of active intermediate species(Cu^(+)-CO)during the reaction.This process ultimately enhanced the catalyst’s activity.展开更多
With the iteration and upgrading of medical technology and the continuous growth of public health demands,the quality of nursing services has become a core indicator for measuring the effectiveness of the medical syst...With the iteration and upgrading of medical technology and the continuous growth of public health demands,the quality of nursing services has become a core indicator for measuring the effectiveness of the medical system.The clinical practice ability of nursing staff is directly related to the safety of patient diagnosis and treatment and the rehabilitation process.However,the current clinical nursing talent training model is facing bottlenecks such as limited practical scenarios and fragmented case cognition.This study focuses on the teaching application of augmented reality(AR)technology in hospital Settings and systematically reviews the research progress on the improvement of clinical practice ability of trainee nurses based on the AR immersive teaching model.By constructing a clinical teaching scenario that integrates virtual and real,AR technology can dynamically simulate complex case handling processes and enhance nursing students’three-dimensional cognition of condition assessment,operation norms,and emergency plans.Hospitals,as the core base for practical teaching,can effectively shorten the connection cycle between theoretical teaching and clinical practice by integrating AR technology,improve the clinical practice level of trainee nurses,and provide an innovative model for optimizing the path of clinical nursing talent cultivation.展开更多
We report the successful application of a surgical and medical co‐management(SMC)strategy in an 82‐year‐old man with hemophilia A(HA)undergoing pancreaticoduodenectomy for pancreatic head carcinoma.No major complic...We report the successful application of a surgical and medical co‐management(SMC)strategy in an 82‐year‐old man with hemophilia A(HA)undergoing pancreaticoduodenectomy for pancreatic head carcinoma.No major complications or perioperative bleeding occurred.Optimal management of HA patients undergoing major surgery requires multidisciplinary coordination to avoid postoperative complications.The SMC team integrates internists(who assess chronic disease status,adjust medications,and determine best hemostatic therapies)and surgeons(who evaluate the surgical feasibility of procedures and rely on advanced surgical skills)to improve perioperative planning to minimize complications and promote recovery.This case illustrates the utility of a shift from passive and conservative treatment to active and preventive treatment and highlights the value of SMC in many complex clinical situations.展开更多
The efficacy and safety of bevacizumab with modified irinotecan,leucovorin bolus,and 5-fluorouracil intravenous infusion(mIFL) in the first-line treatment of metastatic colorectal cancer(mCRC) has not been well evalua...The efficacy and safety of bevacizumab with modified irinotecan,leucovorin bolus,and 5-fluorouracil intravenous infusion(mIFL) in the first-line treatment of metastatic colorectal cancer(mCRC) has not been well evaluated in randomized clinical trials in Chinese patients.We conducted a phrase Ⅲ trial in which patients with previously untreated mCRC were randomized 2:1 to the mIFL [irinotecan(125 mg/m2),leucovorin(20 mg/m2) bolus,and 5-fluorouracil intravenous infusion(500 mg/m2) weekly for four weeks every six weeks] plus bevacizumab(5 mg/kg every two weeks) group and the mIFL group,respectively.Co-primary objectives were progression-free survival(PFS) and 6-month PFS rate.In total,214 patients were enrolled.Our results showed that addition of bevacizumab to mIFL significantly improved median PFS(4.2 months in the mIFL group vs.8.3 months in the bevacizumab plus mIFL group,P < 0.001),6-month PFS rate(25.0% vs.62.6%,P < 0.001),median overall survival(13.4 months vs.18.7 months,P = 0.014),and response rate(17% vs.35%,P = 0.013).Grades 3 and 4 adverse events included diarrhea(21% in the mIFL group and 26% in the bevacizumab plus mIFL group) and neutropenia(19% in the mIFL group and 33% in the bevacizumab plus mIFL group).No wound-healing complications or congestive heart failure occurred.Our results suggested that bevacizumab plus mIFL is effective and well tolerated as first-line treatment for Chinese patients with mCRC.Clinical benefit and safety profiles were consistent with those observed in pivotal phase Ⅲ trials with mainly Caucasian patients.展开更多
Background Fall and serious fall injuries have become a major health concern for elders. Many factors including blood pressure and anti-hypertensive medication application were reported as hazards of fall. The purpose...Background Fall and serious fall injuries have become a major health concern for elders. Many factors including blood pressure and anti-hypertensive medication application were reported as hazards of fall. The purpose of this study was to determine if age related systemic functional decline related with increased fall risks in elderly patients with hypertension. Methods A total of 342 elderly hypertension patients （age 79.5 ＋ 6.7 years, male 63.8%） were recruited to the study. Comprehensive geriatric assessment （CGA）, including measurements about activity of daily living （ADL）, nutrition, cognition, depression, numbers of prescription medication and number of clinical diagnosis, was conducted to evaluate the physical and mental status of each participants. Fall risk was evaluated by Morse fall scale, Tinetti perform- ance oriented mobility assessment （POMA） and history of fall in the recent years. Participants were grouped into tertiles according to CGA score. Correlation between CGA and fall risk was analyzed through SPSS 18.0. Results Participants with higher CGA score were likely to be older, had a lower body mass index （BMI）, and a higher prevalence of cardiovascular disease, chronic obstructive pulmonary disease （COPD）, cerebrovascular disease and osteoarthropathia. Participants in higher tertile of CGA score got increased prevalence of fall risk than those in lower tertile （P 〈 0.01 T3 vs. T1, P 〈 0.01 T3 vs. T2）. Correlation analysis and regression analysis showed significant association between CGA and Morse fall scale （P 〈 0.001）, as well as CGA and POMA （P 〈 0.001）. Meanwhile, CGA components also showed co-relationships with increase fall risks. After adjusting age, BMI, benzodiazepine use, cardiovascular disease, cerebrovascular disease, COPD and osteoarthropathia, both history of fall in the recent year and rising Morse fall scale were significantly associated with ADL im- pairment （OR： 2.748, 95%CI： 1.598-4.725）, （OR： 3.310, 95%CI： 1.893-5.788）. Decreased Tinetti POMA score was associated with Mini-Mental State Examination （MMSE） （OR： 4.035, 95%CI： 2.100-7.751）, ADL （OR： 2.380, 95%CI： 1.357-4.175） and shortened MNA form （MNA-SF） impairment （OR： 2.692, 95%CI： 1.147-6.319）. Conclusions In elderly adults with hypertension, impaired physical and mental function is associated with increased fall risk. Further study is required to investigate possible mediators for the association and effec- tive interventions.展开更多
Levodopa is the gold-standard treatment for Parkinson's disease.However,although it alleviates the clinical symptoms,it cannot delay the progressive apoptosis of dopaminergic neurons or prevent motor complications...Levodopa is the gold-standard treatment for Parkinson's disease.However,although it alleviates the clinical symptoms,it cannot delay the progressive apoptosis of dopaminergic neurons or prevent motor complications in the long term.In the present study,we investigated the effect of Shudipingchan granule on neuronal apoptosis in a rat model of Parkinson's disease,established by injecting 6-hydroxydopamine into the substantia nigra pars compacta and ventral tegmental area.We then administered levodopa(20 mg/kg intraperitoneally,twice daily)with or without Shudipingchan granule(7.5 mL/kg intragastrically,twice daily),for 4 weeks.The long-term use of levodopa accel-erated apoptosis of nigral cells and worsened behavioral symptoms by activating the extracellular signal-regulated kinase pathway and downstream apoptotic factors.However,administration of Shudipingchan granule with levodopa reduced expression of phosphorylated extracellular signal-regulated kinase 1/2 and Bax,increased tyrosine hydroxylase and Bcl-2,reduced apoptosis in the substantia nigra,and markedly improved dyskinesia.These findings suggest that Shudipingchan granule suppresses neuronal apoptosis by inhibiting the hyper-phosphorylation of extracellular signal-regulated kinase and downregulating expression of anti-apoptotic genes.Shudipingchan granule,used in combination with levodopa,can effectively reduce the symptoms of Parkinson's disease.展开更多
Santanghu Coalfield is the largest integrated coalfield exploration area in China. The major coal seams developing in Xishanyao Formation (Middle Jurassic) are the high-quality steam coals characterized by large thick...Santanghu Coalfield is the largest integrated coalfield exploration area in China. The major coal seams developing in Xishanyao Formation (Middle Jurassic) are the high-quality steam coals characterized by large thickness, favorable horizontal continuity and high coal quality. In this paper, twenty-two samples were collected from the three typical boreholes in Hanshuiquan disirict, representing the 11 coal seam sequences (7#,8#,9#,13#, 14#,15#,17#,18#,19#,20#,22#), respectively. The petrographic characteristics of the coal-bearing sequence in Xishanyao Formation were firstly summarized systematicly, and then the coal-forming swamp characteristics and succession mechanism of the coal seam in Xishanyao Formation were defined by analyzing the samples. The maceral composition, structure, geochemical and geophysical characteristics of coal are in eluded in original genetic criteria of coal-forming swamp an alysis. And the composition of coal petrography, maceral and microlithotype are the most frequently used parameters. Coal is composed of microscopic constituents and inorganic substances. The Xishanyao Formation maceral mainly consists of vitrinite (65.74%-97.01 %), inert i nite (1.93%-34%), and the exinite shows the mode of regular change. The coal-forming swamp in Xishanyao Formation possesses the characteristics of mainly marsh, wet forest swamp facies, and shallow water covered forest swamp facies, and a few of coal seams distribute in the deep water covered forest swamp facies. In addition, the sporopollens in Xishanyao Formation are mainly Pinaceae evergreen broad leaf and needle-leaved plants. Osmundaceae, Cyatheaceae and Lygodiaceae, indicating that the warm and humid tropic-subtropical climate conductive to the persistent growth of coal-forming plants in the Middle Jurassic. The coal-forming swamp shows the characteristics of vertically upward fluctuation through the periodic transition. It indicates a shallow-deep-shallow change process of the water covered depth in the swamp. This is the prin ci pal factor for the formation of the high-quality and continuous coal seam in Hanshuiquan district, Santanghu Coalfield.展开更多
Oxidative desulfurization(ODS)has been proved to be an efficient strategy for the production of clean fuel oil.Numerous metal-based materials have been employed as excellent ODS catalysts,but being hindered by their h...Oxidative desulfurization(ODS)has been proved to be an efficient strategy for the production of clean fuel oil.Numerous metal-based materials have been employed as excellent ODS catalysts,but being hindered by their high-cost and potential secondary pollution.In this work,we employed graphene analogous hexagonal boron nitride(h-BN)as a metal-free catalyst for ODS with hydrogen peroxide(H2O2)as the oxidant.The h-BN catalyst was characterized and proved to be a few-layered structure with relatively high specific surface areas.The h-BN catalyst showed a 99.4%of sulfur removal in fuel oil under the optimized reaction conditions.Besides,the h-BN can be recycled for 8 times without significant decrease in the catalytic performance.Detailed mechanism analysis found that it is the boron radicals in h-BN activated H2O2 to generate·OH species,which can readily oxidize sulfides to corresponding sulfones for separation.This work would provide another choice in choosing metal-free catalysts for ODS.展开更多
Construction of catalysts with integral structure for oxidative reaction process is an essential promotion to catalysts in industrial application.In this work,a 3D printing method was employed to prepare 3D printed sp...Construction of catalysts with integral structure for oxidative reaction process is an essential promotion to catalysts in industrial application.In this work,a 3D printing method was employed to prepare 3D printed spheres(3D-PSs),followed by carbonization to form 3D carbon spheres(3D-CSs).Then,a 3D-CSs supported phosphotungstic acid(HPW/3D-CSs)was prepared for deep oxidative desulfurization.Compared with traditional powder catalysts,the as-prepared catalyst is easy to be operated and separated from oil products.The supported catalyst possesses excellent catalytic performance and the removal of DBT,4-MDBT and 4,6-DMDBT in fuel oil,reaching^100%of sulfur removal.The effects of various experimental parameters on desulfurization efficiency were considered to optimize reaction conditions.Moreover,the catalyst shows excellent thermal and chemical stability,with no obvious decrease in desulfurization activity after 5 cycles.GC–MS analysis indicates DBT sulfone was the solely oxidized product of DBT.展开更多
The synergy effect between different components has attracted widespread attentions because of improved activity, selectivity and stability than single component. In this paper, we fabricated mesoporous hybrid dual-me...The synergy effect between different components has attracted widespread attentions because of improved activity, selectivity and stability than single component. In this paper, we fabricated mesoporous hybrid dual-metal Co and Fe containing metallic organic framework（Co/Fe-MOF）, Fe-MOF,and Co-MOF in the ionic liquid（IL）/supercritical CO2（SC）/surfactant emulsion system, and then studied the electrochemical properties of the three MOFs systematically. Experiment results indicate that, by taking advantages of coexistence of double metal, hybrid bi-metal Co/Fe-MOF exhibits the highest specific capacitance and the best cycling stability, with specific capacitance to 319.5 F/g at 1 A/g, 1.4 and 4 times for single Co-MOF and Fe-MOF, respectively.展开更多
Polymorphisms in DNA repair genes may alter DNA repair capacity and,consequently,lead to genetic instability and carcinogenesis.Several studies have investigated the association of the Asp312 Asn and Lys751 Gln polymo...Polymorphisms in DNA repair genes may alter DNA repair capacity and,consequently,lead to genetic instability and carcinogenesis.Several studies have investigated the association of the Asp312 Asn and Lys751 Gln polymorphisms in the xeroderma pigmentosum complementation group D {XPD) gene with the risk of non-Hodgkin's lymphoma(NHL),but the conclusions have been inconsistent.Therefore,we performed this meta-analysis to more precisely estimate these relationships.A systematic literature search was performed using the PubMed,Embase,and Chinese Biomedical(CBM) databases.Ultimately,6 studies of Asp312 Asn,comprising 3,095 cases and 3,306 controls,and 7studies of Lys751 Gln,consisting of 3,249 cases and 3,676 controls,were included.Pooled odds ratios(ORs) and 95%confidence intervals(CIs) were calculated to assess the strength of each association.Overall,no association was observed between the Asp312 Asn polymorphism and NHL risk(homozygous:OR = 1.11,95%CI = 0.94-1.32;heterozygous:OR = 1.00,95%CI = 0.89-1.11;recessive:OR = 1.12,95%CI = 0.95-1.31;dominant:OR = 1.02,95%CI = 0.92-1.13;and allele comparison:OR = 1.04,95%CI = 0.96-1.12) or between the Lys751 Gln polymorphism and NHL risk(homozygous:OR = 0.97,95%CI = 0.83-1.15;heterozygous:OR = 0.96,95%CI = 0.86-1.06;recessive:OR = 1.00,95%CI = 0.86-1.16;dominant:OR = 0.96,95%CI = 0.87-1.06;and allele comparison:OR = 0.98,95%CI = 0.91-1.05).Furthermore,subgroup analyses did not reveal any association between these polymorphisms and ethnicity,the source of the controls,or the NHL subtype.These results indicated that neither the Asp312 Asn nor Lys751 Gln XPD polymorphism was related to NHL risk.Large and well-designed prospective studies are required to confirm this finding.展开更多
AIM： To explore the influence of angiostatin up-regulation on the biologic behavior of gallbladder carcinoma cells in vitro and in vitro, and the potential value of angiostatin gene therapy for gallbladder carcinoma....AIM： To explore the influence of angiostatin up-regulation on the biologic behavior of gallbladder carcinoma cells in vitro and in vitro, and the potential value of angiostatin gene therapy for gallbladder carcinoma. METHODS： A eukaryotic expression vector of pcDNA3.1（＋） containing murine angiostatin was constructed and identified by restriction endonuclease digestion and sequencing. The recombinant vector pcDNA3.1-angiostatin was transfected into human gallbladder carcinoma cell line GBC-SD with Upofectamine 2000, and paralleled with the vector and mock control. The resistant clone was screened by G418 filtration. Angiostatin transcription and protein expression were examined by RT-PCR, immunofluorescence and Western-blot. The supernatant was collected to treat endothelial cells. Cell proliferation and growth in vitro were observed under microscope. RESULTS： Murine angiostatin cDNA was successfully cloned into the eukaryotic expression vector pcDNA3.1 （＋）. AFter 14 d of transfection and selection with G418, macroscopic resistant cell cloning was formed in the experimental group transfected with pcDNA 3.1（＋）-angiostatin and vector control. But untreated cells died in the mock control. Angiostatin was detected by RT-PCR and protein expression was detected in the experimental group by immunofluorescence and Western-blot. Cell proliferation and growth in v/tro in the three groups were observed respectively under microscope. No significant difference was observed in the growth speed of GBC- SD cells between groups that were transfected with and without angiostatin. After treatment with supernatant, significant differences were observed in endothelial cell （ECV-304） growth in vitro. The cell proliferation and growth were inhibited. CONCLUSION： Angiostatin does not directly inhibit human gallbladder carcinoma cell proliferation and growth in vitro, but the secretion of angiostatin inhabits endothelial cell proliferation and growth.展开更多
Oleanolic acid(OA)is a pentacyclic triterpenoid compound with extensive biological effects,such as anti-inflammatory and anticancer activities.However,the application of OA in chemotherapy is hampered by its poor solu...Oleanolic acid(OA)is a pentacyclic triterpenoid compound with extensive biological effects,such as anti-inflammatory and anticancer activities.However,the application of OA in chemotherapy is hampered by its poor solubility and severe adverse effects.To solve the problems,we developed a self-assembled nanoparticle platform based on amphiphilic oleanolic acid polyprodrug,poly[oligo(ethylene glycol)methyl ether methacrylate]-b-poly(oleanolic acid methacrylate)(POEGMA-b-POAMA),encapsulating 10-hydroxycamptothecin(HCPT)to achieve efficient cancer therapy.The polyprodrug was prepared via reversible addition-fragmentation chain transfer polymerization(RAFT),and could selfassemble to prepare POEGMA-b-POAMA/HCPT nanoparticles(NPs).The obtained nanoparticles exhibited appropriate particle size,excellent drug stability,good drug loading capacity,and high drug loading efficiency.In vitro drug release indicated that the drug release was prolonged to 132 h.The POEGMA-b-POAMA/HCPT NPs enhanced cell cytotoxicity in 4T1 cells and MCF-7 cells and could be efficiently uptaken by 4T1 cells.Furthermore,in vivo antitumor efficiency showed that the POEGMA-b-POAMA/HCPT NPs had great antitumor efficiency with considerably low adverse effects in the treatment of the 4T1 mouse breast tumor xenograft tumor.Therefore,POEGMA-b-POAMA/HCPT NPs provide great potential as a platform for drug delivery applications.展开更多
Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173...Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173 eyes) were studied. The PhNR was elicited using a white stimuli on a white background..The mean deviation (MD) and pattern standard deviation (PSD) of the visual field were measured using standard automated perimetry (SAP)..Spectral domain optical coherence tomography (SD-OCT).was used to measure the mean thickness of the retinal nerve fiber layer (RNFL). Results:.In the glaucoma group,.as compared to the normal group,.the amplitudes of a-waves,.b-waves and PhNR were significantly smaller (P<0.001), and the PhNR implicit time was significantly longer.(P=0.004). The MD, PSD and mean thickness of the RNFL were significantly correlated with the amplitude of the PhNR (P<0.001). The area under the receiver operating characteristic curve (AUCs) for the amplitudes of a-waves,.b-waves and PhNR were 0.853,.0.830 and 0.918, respectively. When the specificity was ≥95%, the sensitivities were 60.4%, 54.2% and 85.4% respectively. Conclusion:The PhNR amplitude was reduced even when the loss in visual field sensitivity was mild, which suggests that PhNR might be a useful indicator of early glaucoma disease.展开更多
A metal-free N-hydroxyphthalimide/hexagonal boron nitride(NHPI/h-BN)catalytic system was developed for deep oxidative desulfurization(ODS)of fuel oils.Detailed experiments find that the heterogenization process of loa...A metal-free N-hydroxyphthalimide/hexagonal boron nitride(NHPI/h-BN)catalytic system was developed for deep oxidative desulfurization(ODS)of fuel oils.Detailed experiments find that the heterogenization process of loading NHPI on h-BN not only benefits to the dispersion and utilization of NHPI,but also can significantly promote the catalytic performance.By employing NHPI/h-BN as the catalyst,azodiisobutyronitrile(AIBN)as the metal-free initiator,a 95%conversion of dibenzothiophene(DBT)can be acquired under the reaction conditions of 120°C and atmospheric pressure with molecular oxygen(O_(2))as oxidant.Moreover,the heterogenization is convenient for the regeneration of the catalyst with>94%DBT conversion after being recycled seven times.Characterizations illustrate that the promoted catalytic activity along with the regenerability originate from the interactions between NHPI and h-BN.The catalytic mechanism study shows that molecular oxygen is readily activated by the NHPI/h-BN to form a superoxide radical(O_(2)^(·-)),which oxidize DBT to DBTO2 for desulfurization.展开更多
Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to ev...Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to evaluate the relationship between NER gene polymorphisms and the risk of hepatoblastoma in Eastern Chinese Han children.Methods:In this five-center case-control study,we enrolled 966 subjects from East China(193 hepatoblastoma patients and 773 healthy controls).The TaqMan method was used to genotype 19 single nucleotide polymorphisms(SNPs)in NER pathway genes,including ERCC1,XPA,XPC,XPD,XPF,and XPG.Then,multivariate logistic regression analysis was performed,and odds ratios(ORs)and 95%confidence intervals(95%CIs)were utilized to assess the strength of associations.Results:Three SNPs were related to hepatoblastoma risk.XPC rs2229090 and XPD rs3810366 significantly contributed to hepatoblastoma risk according to the dominant model(adjusted OR=1.49,95%CI=1.07−2.08,P=0.019;adjusted OR=1.66,95%CI=1.12−2.45,P=0.012,respectively).However,XPD rs238406 conferred a significantly decreased risk of hepatoblastoma under the dominant model(adjusted OR=0.68,95%CI=0.49−0.95;P=0.024).Stratified analysis demonstrated that these significant associations were more prominent in certain subgroups.Moreover,there was evidence of functional implications of these significant SNPs suggested by online expression quantitative trait loci(eQTLs)and splicing quantitative trait loci(sQTLs)analysis.Conclusions:In summary,NER pathway gene polymorphisms(XPC rs2229090,XPD rs3810366,and XPD rs238406)are significantly associated with hepatoblastoma risk,and further research is required to verify these findings.展开更多
Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers fro...Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers from interference,which causes serious problems,such as wasting energy and degrading throughput.In reality,not all of the sensors in WBAN need to be active at the same time.Therefore,they can be divided into different groups so that each group works in turn to avoid interference.In this paper,a Nest-Based WBAN Scheduling(NBWS)algorithm is proposed to cluster sensors of the same types in a single or multiple WBANs into different groups to avoid interference.Particularly,we borrow the graph coloring theory to schedule all groups to work using a Time Division for Multimodal Sensor(TDMS)group scheduling model.Both theoretical analysis and experimental results demonstrate that the proposed NBWS algorithm performs better in terms of frequency of collisions,transmission delay,system throughput,and energy consumption compared to the counterpart methods.展开更多
Prolactin(PRL)is a polypeptide hormone that is mainly synthesized and secreted by the lactotroph cells of the pituitary.There are two main isoforms of PRL:23-kDa PRL(named full-l ength PRL)and vasoinhibins(including ...Prolactin(PRL)is a polypeptide hormone that is mainly synthesized and secreted by the lactotroph cells of the pituitary.There are two main isoforms of PRL:23-kDa PRL(named full-l ength PRL)and vasoinhibins(including 5.6–18 kDa fragments).Both act as circulating hormones and cytokines to stimulate or inhibit vascular formation at different stages and neovascularization,including endothelial cell proliferation and migration,protease production,and apoptosis.However,their effects on vascular function and cardiovascular diseases are different or even contrary.In addition to the structure,secretion regulation,and signal transduction of PRL/vasoinhibins,this review focuses on the pathological mechanism and clinical significance of PRL/vasoinhibins in cardiovascular diseases.展开更多
基金supported by the National Natural Science Foundation of China(82461160264,82474056,and 82104124)the grant from the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0502605)+2 种基金the Science and Technology Development Fund,Macao SAR(FDCT 0025/2021/A1)the Shanghai Municipal Health Commission(2022XD037)the Shanghai Magnolia Talent Plan Pujiang Project(23PJD113)。
文摘Geniposide,the principal active iridoid glucoside ingredient in Fructus gardeniae used in numerous traditional Chinese clinical prescriptions,has been shown to cause herbal hepatotoxicity because of its glycone metabolite genipin.This study explored the role of gut microbiota in alleviating geniposide hepatotoxicity with isoflavones in soy products.Metabolic profiling using ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q/TOF-MS)revealed two metabolic pathways and six main forms of geniposides in vivo.Enzyme inhibitor experiments have shown that isoflavones alter geniposide metabolism by mediating specific enzymes,includingβ-glucosidase(β-GC)and sulfotransferase(SULT),in an established pseudo-sterile rat model.Isoflavones pretreatment by gavage for three weeks optimized the structure of the gut microbiota was linked to the regulation of key metabolic enzymes.Furthermore,experiments involving fecal microbiota transplantation(FMT)established the direct contribution of the gut microbiota to the regulation of enzyme activities and geniposide metabolism.This study demonstrated that isoflavones in soy products regulated the metabolic enzymes of geniposode dependent on gut microbiota,especially Lactobacillus spp.,which was further verified in our clinical trials analyzed using 16S ribosomal RNA(rRNA)and metagenomic sequencing,thus regulating geniposide metabolism.Furthermore,as dominant beneficial bacterium,Lactobacillus spp.were discovered to be promising microbial targets for the better management of geniposide hepatotoxicity.These findings provide valuable insights for the prevention and intervention of drug-induced liver injury.
基金financially supported by the National Natural Science Foundation of China(52002157).
文摘Although organic compounds are considered to be promising electrode materials with their remarkable characteristics such as diverse structures,design controllability,and environmental friendliness,their low charge-transfer capability and limited cycling durability hinder their application in aqueous proton batteries.Herein,we prepared a noncovalent phenazine-based graphene aerogel(H/G)composite for aqueous proton storage,which is realized by redox-active Hexaazatrinaphthalene(HATN)organic compound combined with conductive reduced graphene oxide(rGO).The integration of rGO into HATN not only effectively optimizes the electronic structure of the H/G composite to enhance its electrochemical activity,but also the favorable noncovalent π-π interaction existed between HATN and rGO provides a stable structure for fast electron transportation.The obvious electron transfer in the aerogel composite promotes fast and reversible redox reactions occurred with the imino-active HATN in the composite electrode for proton uptake/removal in an aqueous acidic electrolyte,which are demonstrated by in-situ Fourier transform infrared(FTIR)investigation,theoretical calculations and experimental measurements.Therefore,it can deliver a fast,stable and efficient aqueous proton storage behavior with a large specific capacity of 274 mA·h·g^(-1) and considerable calendar life with~100%capacity retention after 3000 cycles,surpassing previously reported proton-based organic electrodes in aqueous acidic electrolytes.Furthermore,an outstanding soft-package aqueous proton(APB)has been fabricated with considerable long-term cycling stability.
基金supported by the National Key R&D Program of China(No.2022YFB3805504)the National Natu-ral Science Foundation of China(No.22078089)+2 种基金the Shanghai Pilot Program for Basic Research(No.22TQ1400100-7)the Basic Research Program of Science and Technology Commission of Shanghai Munici-pality(No.22JC1400600)the Fundamental Research Funds for the Central Universities.
文摘Carbon monoxide(CO)oxidation is crucial for pollutant removal and hydrogen purification.In recent years,copper–cerium(Cu–Ce)-mixed oxide catalysts have attracted significant attention due to their excellent activity and stability in CO oxida-tion.This study presents an innovative,environmentally friendly electrosynthesis method for producing stable,structured Cu–Ce catalysts in mesh form.This approach addresses the limitations of traditional pellet catalysts,such as fragility and poor thermal conductivity.The results demonstrated that incorporating cerium(Ce)enhanced the catalytic activity for CO oxidation threefold.A series of in situ characterizations revealed that the introduction of Ce led to the formation of a Cu–Ce mixed oxide solid solution,which significantly improved catalytic performance.Furthermore,higher pretreatment tem-peratures facilitated the decomposition of Ce compounds(nitrate and hydroxide),which promotes the formation of Cu–Ce solid solutions and increases the concentration of active intermediate species(Cu^(+)-CO)during the reaction.This process ultimately enhanced the catalyst’s activity.
文摘With the iteration and upgrading of medical technology and the continuous growth of public health demands,the quality of nursing services has become a core indicator for measuring the effectiveness of the medical system.The clinical practice ability of nursing staff is directly related to the safety of patient diagnosis and treatment and the rehabilitation process.However,the current clinical nursing talent training model is facing bottlenecks such as limited practical scenarios and fragmented case cognition.This study focuses on the teaching application of augmented reality(AR)technology in hospital Settings and systematically reviews the research progress on the improvement of clinical practice ability of trainee nurses based on the AR immersive teaching model.By constructing a clinical teaching scenario that integrates virtual and real,AR technology can dynamically simulate complex case handling processes and enhance nursing students’three-dimensional cognition of condition assessment,operation norms,and emergency plans.Hospitals,as the core base for practical teaching,can effectively shorten the connection cycle between theoretical teaching and clinical practice by integrating AR technology,improve the clinical practice level of trainee nurses,and provide an innovative model for optimizing the path of clinical nursing talent cultivation.
文摘We report the successful application of a surgical and medical co‐management(SMC)strategy in an 82‐year‐old man with hemophilia A(HA)undergoing pancreaticoduodenectomy for pancreatic head carcinoma.No major complications or perioperative bleeding occurred.Optimal management of HA patients undergoing major surgery requires multidisciplinary coordination to avoid postoperative complications.The SMC team integrates internists(who assess chronic disease status,adjust medications,and determine best hemostatic therapies)and surgeons(who evaluate the surgical feasibility of procedures and rely on advanced surgical skills)to improve perioperative planning to minimize complications and promote recovery.This case illustrates the utility of a shift from passive and conservative treatment to active and preventive treatment and highlights the value of SMC in many complex clinical situations.
文摘The efficacy and safety of bevacizumab with modified irinotecan,leucovorin bolus,and 5-fluorouracil intravenous infusion(mIFL) in the first-line treatment of metastatic colorectal cancer(mCRC) has not been well evaluated in randomized clinical trials in Chinese patients.We conducted a phrase Ⅲ trial in which patients with previously untreated mCRC were randomized 2:1 to the mIFL [irinotecan(125 mg/m2),leucovorin(20 mg/m2) bolus,and 5-fluorouracil intravenous infusion(500 mg/m2) weekly for four weeks every six weeks] plus bevacizumab(5 mg/kg every two weeks) group and the mIFL group,respectively.Co-primary objectives were progression-free survival(PFS) and 6-month PFS rate.In total,214 patients were enrolled.Our results showed that addition of bevacizumab to mIFL significantly improved median PFS(4.2 months in the mIFL group vs.8.3 months in the bevacizumab plus mIFL group,P < 0.001),6-month PFS rate(25.0% vs.62.6%,P < 0.001),median overall survival(13.4 months vs.18.7 months,P = 0.014),and response rate(17% vs.35%,P = 0.013).Grades 3 and 4 adverse events included diarrhea(21% in the mIFL group and 26% in the bevacizumab plus mIFL group) and neutropenia(19% in the mIFL group and 33% in the bevacizumab plus mIFL group).No wound-healing complications or congestive heart failure occurred.Our results suggested that bevacizumab plus mIFL is effective and well tolerated as first-line treatment for Chinese patients with mCRC.Clinical benefit and safety profiles were consistent with those observed in pivotal phase Ⅲ trials with mainly Caucasian patients.
文摘Background Fall and serious fall injuries have become a major health concern for elders. Many factors including blood pressure and anti-hypertensive medication application were reported as hazards of fall. The purpose of this study was to determine if age related systemic functional decline related with increased fall risks in elderly patients with hypertension. Methods A total of 342 elderly hypertension patients （age 79.5 ＋ 6.7 years, male 63.8%） were recruited to the study. Comprehensive geriatric assessment （CGA）, including measurements about activity of daily living （ADL）, nutrition, cognition, depression, numbers of prescription medication and number of clinical diagnosis, was conducted to evaluate the physical and mental status of each participants. Fall risk was evaluated by Morse fall scale, Tinetti perform- ance oriented mobility assessment （POMA） and history of fall in the recent years. Participants were grouped into tertiles according to CGA score. Correlation between CGA and fall risk was analyzed through SPSS 18.0. Results Participants with higher CGA score were likely to be older, had a lower body mass index （BMI）, and a higher prevalence of cardiovascular disease, chronic obstructive pulmonary disease （COPD）, cerebrovascular disease and osteoarthropathia. Participants in higher tertile of CGA score got increased prevalence of fall risk than those in lower tertile （P 〈 0.01 T3 vs. T1, P 〈 0.01 T3 vs. T2）. Correlation analysis and regression analysis showed significant association between CGA and Morse fall scale （P 〈 0.001）, as well as CGA and POMA （P 〈 0.001）. Meanwhile, CGA components also showed co-relationships with increase fall risks. After adjusting age, BMI, benzodiazepine use, cardiovascular disease, cerebrovascular disease, COPD and osteoarthropathia, both history of fall in the recent year and rising Morse fall scale were significantly associated with ADL im- pairment （OR： 2.748, 95%CI： 1.598-4.725）, （OR： 3.310, 95%CI： 1.893-5.788）. Decreased Tinetti POMA score was associated with Mini-Mental State Examination （MMSE） （OR： 4.035, 95%CI： 2.100-7.751）, ADL （OR： 2.380, 95%CI： 1.357-4.175） and shortened MNA form （MNA-SF） impairment （OR： 2.692, 95%CI： 1.147-6.319）. Conclusions In elderly adults with hypertension, impaired physical and mental function is associated with increased fall risk. Further study is required to investigate possible mediators for the association and effec- tive interventions.
基金financially supported by the National Natural Science Foundation of China,No.81302926,30472207the Major Project of Shanghai Science and Technology Commission of China,No.15401970100the Shanghai Municipal Commission of Health and Family Planning of China,No.ZY3-RCPY-2-2005
文摘Levodopa is the gold-standard treatment for Parkinson's disease.However,although it alleviates the clinical symptoms,it cannot delay the progressive apoptosis of dopaminergic neurons or prevent motor complications in the long term.In the present study,we investigated the effect of Shudipingchan granule on neuronal apoptosis in a rat model of Parkinson's disease,established by injecting 6-hydroxydopamine into the substantia nigra pars compacta and ventral tegmental area.We then administered levodopa(20 mg/kg intraperitoneally,twice daily)with or without Shudipingchan granule(7.5 mL/kg intragastrically,twice daily),for 4 weeks.The long-term use of levodopa accel-erated apoptosis of nigral cells and worsened behavioral symptoms by activating the extracellular signal-regulated kinase pathway and downstream apoptotic factors.However,administration of Shudipingchan granule with levodopa reduced expression of phosphorylated extracellular signal-regulated kinase 1/2 and Bax,increased tyrosine hydroxylase and Bcl-2,reduced apoptosis in the substantia nigra,and markedly improved dyskinesia.These findings suggest that Shudipingchan granule suppresses neuronal apoptosis by inhibiting the hyper-phosphorylation of extracellular signal-regulated kinase and downregulating expression of anti-apoptotic genes.Shudipingchan granule,used in combination with levodopa,can effectively reduce the symptoms of Parkinson's disease.
基金National Natural Science Foundation Project (Grant No. 41662010)Xinjiang Uygur Autonomous Region University Scientific Research Program For Young Teachers Research And Cultivation Fund Project (Grant No. XJEDU20I6S038).
文摘Santanghu Coalfield is the largest integrated coalfield exploration area in China. The major coal seams developing in Xishanyao Formation (Middle Jurassic) are the high-quality steam coals characterized by large thickness, favorable horizontal continuity and high coal quality. In this paper, twenty-two samples were collected from the three typical boreholes in Hanshuiquan disirict, representing the 11 coal seam sequences (7#,8#,9#,13#, 14#,15#,17#,18#,19#,20#,22#), respectively. The petrographic characteristics of the coal-bearing sequence in Xishanyao Formation were firstly summarized systematicly, and then the coal-forming swamp characteristics and succession mechanism of the coal seam in Xishanyao Formation were defined by analyzing the samples. The maceral composition, structure, geochemical and geophysical characteristics of coal are in eluded in original genetic criteria of coal-forming swamp an alysis. And the composition of coal petrography, maceral and microlithotype are the most frequently used parameters. Coal is composed of microscopic constituents and inorganic substances. The Xishanyao Formation maceral mainly consists of vitrinite (65.74%-97.01 %), inert i nite (1.93%-34%), and the exinite shows the mode of regular change. The coal-forming swamp in Xishanyao Formation possesses the characteristics of mainly marsh, wet forest swamp facies, and shallow water covered forest swamp facies, and a few of coal seams distribute in the deep water covered forest swamp facies. In addition, the sporopollens in Xishanyao Formation are mainly Pinaceae evergreen broad leaf and needle-leaved plants. Osmundaceae, Cyatheaceae and Lygodiaceae, indicating that the warm and humid tropic-subtropical climate conductive to the persistent growth of coal-forming plants in the Middle Jurassic. The coal-forming swamp shows the characteristics of vertically upward fluctuation through the periodic transition. It indicates a shallow-deep-shallow change process of the water covered depth in the swamp. This is the prin ci pal factor for the formation of the high-quality and continuous coal seam in Hanshuiquan district, Santanghu Coalfield.
基金All authors appreciate the financial support from the National Key R&D Program of China(2017YFB0306504)the National Natural Science Foundation of China(No.21722604,21878133 and 21908082)+2 种基金China Postdoctoral Science Foundation(No.2019M651743)Natural Science Foundation of Jiangsu Province(BK20190852,BK20190854)Natural Science Foundation for Jiangsu Colleges and Universities(19KJB530005).
文摘Oxidative desulfurization(ODS)has been proved to be an efficient strategy for the production of clean fuel oil.Numerous metal-based materials have been employed as excellent ODS catalysts,but being hindered by their high-cost and potential secondary pollution.In this work,we employed graphene analogous hexagonal boron nitride(h-BN)as a metal-free catalyst for ODS with hydrogen peroxide(H2O2)as the oxidant.The h-BN catalyst was characterized and proved to be a few-layered structure with relatively high specific surface areas.The h-BN catalyst showed a 99.4%of sulfur removal in fuel oil under the optimized reaction conditions.Besides,the h-BN can be recycled for 8 times without significant decrease in the catalytic performance.Detailed mechanism analysis found that it is the boron radicals in h-BN activated H2O2 to generate·OH species,which can readily oxidize sulfides to corresponding sulfones for separation.This work would provide another choice in choosing metal-free catalysts for ODS.
基金financially supported by the National Natural Science Foundation of China(Nos.21722604,21576122,21878133)China Postdoctoral Science Foundation(No.2019M651743)。
文摘Construction of catalysts with integral structure for oxidative reaction process is an essential promotion to catalysts in industrial application.In this work,a 3D printing method was employed to prepare 3D printed spheres(3D-PSs),followed by carbonization to form 3D carbon spheres(3D-CSs).Then,a 3D-CSs supported phosphotungstic acid(HPW/3D-CSs)was prepared for deep oxidative desulfurization.Compared with traditional powder catalysts,the as-prepared catalyst is easy to be operated and separated from oil products.The supported catalyst possesses excellent catalytic performance and the removal of DBT,4-MDBT and 4,6-DMDBT in fuel oil,reaching^100%of sulfur removal.The effects of various experimental parameters on desulfurization efficiency were considered to optimize reaction conditions.Moreover,the catalyst shows excellent thermal and chemical stability,with no obvious decrease in desulfurization activity after 5 cycles.GC–MS analysis indicates DBT sulfone was the solely oxidized product of DBT.
基金financially supported by the National Natural Science Foundation of China (Nos. 21571157, U1604123 and 51173170)outstanding Young Talent Research Fund of Zhengzhou University (No. 1521320001)the Open Project Foundation of Key Laboratory of Advanced Energy Materials Chemistry of Nankai University
文摘The synergy effect between different components has attracted widespread attentions because of improved activity, selectivity and stability than single component. In this paper, we fabricated mesoporous hybrid dual-metal Co and Fe containing metallic organic framework（Co/Fe-MOF）, Fe-MOF,and Co-MOF in the ionic liquid（IL）/supercritical CO2（SC）/surfactant emulsion system, and then studied the electrochemical properties of the three MOFs systematically. Experiment results indicate that, by taking advantages of coexistence of double metal, hybrid bi-metal Co/Fe-MOF exhibits the highest specific capacitance and the best cycling stability, with specific capacitance to 319.5 F/g at 1 A/g, 1.4 and 4 times for single Co-MOF and Fe-MOF, respectively.
基金supported by grants from the National Science Fund for Distinguished Young Scholars(No.81325018)the key project for International Cooperation and Exchange of the National Natural Science Foundation of China(No.81220108022)Special Financial Grant from the China Postdoctoral Science Foundation(No.2014 T70836)
文摘Polymorphisms in DNA repair genes may alter DNA repair capacity and,consequently,lead to genetic instability and carcinogenesis.Several studies have investigated the association of the Asp312 Asn and Lys751 Gln polymorphisms in the xeroderma pigmentosum complementation group D {XPD) gene with the risk of non-Hodgkin's lymphoma(NHL),but the conclusions have been inconsistent.Therefore,we performed this meta-analysis to more precisely estimate these relationships.A systematic literature search was performed using the PubMed,Embase,and Chinese Biomedical(CBM) databases.Ultimately,6 studies of Asp312 Asn,comprising 3,095 cases and 3,306 controls,and 7studies of Lys751 Gln,consisting of 3,249 cases and 3,676 controls,were included.Pooled odds ratios(ORs) and 95%confidence intervals(CIs) were calculated to assess the strength of each association.Overall,no association was observed between the Asp312 Asn polymorphism and NHL risk(homozygous:OR = 1.11,95%CI = 0.94-1.32;heterozygous:OR = 1.00,95%CI = 0.89-1.11;recessive:OR = 1.12,95%CI = 0.95-1.31;dominant:OR = 1.02,95%CI = 0.92-1.13;and allele comparison:OR = 1.04,95%CI = 0.96-1.12) or between the Lys751 Gln polymorphism and NHL risk(homozygous:OR = 0.97,95%CI = 0.83-1.15;heterozygous:OR = 0.96,95%CI = 0.86-1.06;recessive:OR = 1.00,95%CI = 0.86-1.16;dominant:OR = 0.96,95%CI = 0.87-1.06;and allele comparison:OR = 0.98,95%CI = 0.91-1.05).Furthermore,subgroup analyses did not reveal any association between these polymorphisms and ethnicity,the source of the controls,or the NHL subtype.These results indicated that neither the Asp312 Asn nor Lys751 Gln XPD polymorphism was related to NHL risk.Large and well-designed prospective studies are required to confirm this finding.
文摘AIM： To explore the influence of angiostatin up-regulation on the biologic behavior of gallbladder carcinoma cells in vitro and in vitro, and the potential value of angiostatin gene therapy for gallbladder carcinoma. METHODS： A eukaryotic expression vector of pcDNA3.1（＋） containing murine angiostatin was constructed and identified by restriction endonuclease digestion and sequencing. The recombinant vector pcDNA3.1-angiostatin was transfected into human gallbladder carcinoma cell line GBC-SD with Upofectamine 2000, and paralleled with the vector and mock control. The resistant clone was screened by G418 filtration. Angiostatin transcription and protein expression were examined by RT-PCR, immunofluorescence and Western-blot. The supernatant was collected to treat endothelial cells. Cell proliferation and growth in vitro were observed under microscope. RESULTS： Murine angiostatin cDNA was successfully cloned into the eukaryotic expression vector pcDNA3.1 （＋）. AFter 14 d of transfection and selection with G418, macroscopic resistant cell cloning was formed in the experimental group transfected with pcDNA 3.1（＋）-angiostatin and vector control. But untreated cells died in the mock control. Angiostatin was detected by RT-PCR and protein expression was detected in the experimental group by immunofluorescence and Western-blot. Cell proliferation and growth in v/tro in the three groups were observed respectively under microscope. No significant difference was observed in the growth speed of GBC- SD cells between groups that were transfected with and without angiostatin. After treatment with supernatant, significant differences were observed in endothelial cell （ECV-304） growth in vitro. The cell proliferation and growth were inhibited. CONCLUSION： Angiostatin does not directly inhibit human gallbladder carcinoma cell proliferation and growth in vitro, but the secretion of angiostatin inhabits endothelial cell proliferation and growth.
基金This work was financially supported by the National Natural Science Foundation of China(No.21576029)National Key R&D Program of China(No.2017YFD0601205).
文摘Oleanolic acid(OA)is a pentacyclic triterpenoid compound with extensive biological effects,such as anti-inflammatory and anticancer activities.However,the application of OA in chemotherapy is hampered by its poor solubility and severe adverse effects.To solve the problems,we developed a self-assembled nanoparticle platform based on amphiphilic oleanolic acid polyprodrug,poly[oligo(ethylene glycol)methyl ether methacrylate]-b-poly(oleanolic acid methacrylate)(POEGMA-b-POAMA),encapsulating 10-hydroxycamptothecin(HCPT)to achieve efficient cancer therapy.The polyprodrug was prepared via reversible addition-fragmentation chain transfer polymerization(RAFT),and could selfassemble to prepare POEGMA-b-POAMA/HCPT nanoparticles(NPs).The obtained nanoparticles exhibited appropriate particle size,excellent drug stability,good drug loading capacity,and high drug loading efficiency.In vitro drug release indicated that the drug release was prolonged to 132 h.The POEGMA-b-POAMA/HCPT NPs enhanced cell cytotoxicity in 4T1 cells and MCF-7 cells and could be efficiently uptaken by 4T1 cells.Furthermore,in vivo antitumor efficiency showed that the POEGMA-b-POAMA/HCPT NPs had great antitumor efficiency with considerably low adverse effects in the treatment of the 4T1 mouse breast tumor xenograft tumor.Therefore,POEGMA-b-POAMA/HCPT NPs provide great potential as a platform for drug delivery applications.
文摘Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173 eyes) were studied. The PhNR was elicited using a white stimuli on a white background..The mean deviation (MD) and pattern standard deviation (PSD) of the visual field were measured using standard automated perimetry (SAP)..Spectral domain optical coherence tomography (SD-OCT).was used to measure the mean thickness of the retinal nerve fiber layer (RNFL). Results:.In the glaucoma group,.as compared to the normal group,.the amplitudes of a-waves,.b-waves and PhNR were significantly smaller (P<0.001), and the PhNR implicit time was significantly longer.(P=0.004). The MD, PSD and mean thickness of the RNFL were significantly correlated with the amplitude of the PhNR (P<0.001). The area under the receiver operating characteristic curve (AUCs) for the amplitudes of a-waves,.b-waves and PhNR were 0.853,.0.830 and 0.918, respectively. When the specificity was ≥95%, the sensitivities were 60.4%, 54.2% and 85.4% respectively. Conclusion:The PhNR amplitude was reduced even when the loss in visual field sensitivity was mild, which suggests that PhNR might be a useful indicator of early glaucoma disease.
基金the financial support from the National Key R&D Program of China(No.2017YFB0306504)National Natural Science Foundation of China(No.22008094,22178154 and 21878133)+2 种基金Chinese Postdoctoral Science Foundation(No.2019M651743,2020M671364 and 2020M673039)Natural Science Foundation of Jiangsu Province(No.BK20190852)Natural Science Foundation for Jiangsu Colleges and Universities(No.19KJB530005)
文摘A metal-free N-hydroxyphthalimide/hexagonal boron nitride(NHPI/h-BN)catalytic system was developed for deep oxidative desulfurization(ODS)of fuel oils.Detailed experiments find that the heterogenization process of loading NHPI on h-BN not only benefits to the dispersion and utilization of NHPI,but also can significantly promote the catalytic performance.By employing NHPI/h-BN as the catalyst,azodiisobutyronitrile(AIBN)as the metal-free initiator,a 95%conversion of dibenzothiophene(DBT)can be acquired under the reaction conditions of 120°C and atmospheric pressure with molecular oxygen(O_(2))as oxidant.Moreover,the heterogenization is convenient for the regeneration of the catalyst with>94%DBT conversion after being recycled seven times.Characterizations illustrate that the promoted catalytic activity along with the regenerability originate from the interactions between NHPI and h-BN.The catalytic mechanism study shows that molecular oxygen is readily activated by the NHPI/h-BN to form a superoxide radical(O_(2)^(·-)),which oxidize DBT to DBTO2 for desulfurization.
基金supported by grants from the Innovation and Cultivation Fund Project of the Seventh Medical Center,PLA General Hospital(No.QZX-2023-7)Postdoctoral Science Foundation of China(No.2021M691649)Postdoctoral Science Foundation of Jiangsu Province(No.2021K524C).
文摘Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to evaluate the relationship between NER gene polymorphisms and the risk of hepatoblastoma in Eastern Chinese Han children.Methods:In this five-center case-control study,we enrolled 966 subjects from East China(193 hepatoblastoma patients and 773 healthy controls).The TaqMan method was used to genotype 19 single nucleotide polymorphisms(SNPs)in NER pathway genes,including ERCC1,XPA,XPC,XPD,XPF,and XPG.Then,multivariate logistic regression analysis was performed,and odds ratios(ORs)and 95%confidence intervals(95%CIs)were utilized to assess the strength of associations.Results:Three SNPs were related to hepatoblastoma risk.XPC rs2229090 and XPD rs3810366 significantly contributed to hepatoblastoma risk according to the dominant model(adjusted OR=1.49,95%CI=1.07−2.08,P=0.019;adjusted OR=1.66,95%CI=1.12−2.45,P=0.012,respectively).However,XPD rs238406 conferred a significantly decreased risk of hepatoblastoma under the dominant model(adjusted OR=0.68,95%CI=0.49−0.95;P=0.024).Stratified analysis demonstrated that these significant associations were more prominent in certain subgroups.Moreover,there was evidence of functional implications of these significant SNPs suggested by online expression quantitative trait loci(eQTLs)and splicing quantitative trait loci(sQTLs)analysis.Conclusions:In summary,NER pathway gene polymorphisms(XPC rs2229090,XPD rs3810366,and XPD rs238406)are significantly associated with hepatoblastoma risk,and further research is required to verify these findings.
基金the Ningbo International Science and Technology Cooperation Programme(2016D10008)the Ningbo Key Science and Technology plan(2025)projects(2018B10075,2019B10125,2019B10028)+2 种基金the Marine Biotechnology and Marine Engineering Discipline Group(422004582)the Project of Research and Development of Intelligent Resource Allocation and Sharing Platform for Marine Electronic Information Industry(2017GY116)the Key science and technology projects of Zhejiang Province(2020C03064).
文摘Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers from interference,which causes serious problems,such as wasting energy and degrading throughput.In reality,not all of the sensors in WBAN need to be active at the same time.Therefore,they can be divided into different groups so that each group works in turn to avoid interference.In this paper,a Nest-Based WBAN Scheduling(NBWS)algorithm is proposed to cluster sensors of the same types in a single or multiple WBANs into different groups to avoid interference.Particularly,we borrow the graph coloring theory to schedule all groups to work using a Time Division for Multimodal Sensor(TDMS)group scheduling model.Both theoretical analysis and experimental results demonstrate that the proposed NBWS algorithm performs better in terms of frequency of collisions,transmission delay,system throughput,and energy consumption compared to the counterpart methods.
基金National Natural Science Foundation of China,Grant/Award Number:81700045,81870042 and 81900050Natural Science Foundation of Shanghai,Grant/Award Number:22ZR1452400+1 种基金Shanghai Municipal Commission of Health,Grant/Award Number:20204Y0384Shanghai Pulmonary Hospital,Grant/Award Number:fk18003 and fkyq1605。
文摘Prolactin(PRL)is a polypeptide hormone that is mainly synthesized and secreted by the lactotroph cells of the pituitary.There are two main isoforms of PRL:23-kDa PRL(named full-l ength PRL)and vasoinhibins(including 5.6–18 kDa fragments).Both act as circulating hormones and cytokines to stimulate or inhibit vascular formation at different stages and neovascularization,including endothelial cell proliferation and migration,protease production,and apoptosis.However,their effects on vascular function and cardiovascular diseases are different or even contrary.In addition to the structure,secretion regulation,and signal transduction of PRL/vasoinhibins,this review focuses on the pathological mechanism and clinical significance of PRL/vasoinhibins in cardiovascular diseases.
