BACKGROUND Liver cancer is one of the most common malignant tumors of the digestive system,and early detection and effective treatment are crucial for improving the prognosis.Microwave ablation(MWA)has shown promising...BACKGROUND Liver cancer is one of the most common malignant tumors of the digestive system,and early detection and effective treatment are crucial for improving the prognosis.Microwave ablation(MWA)has shown promising results as a local therapeutic method for liver cancer;however,further improvement of its efficacy remains a key focus of current research.AIM To evaluate the clinical efficacy of Linggui Zhugan decoction combined with MWA for the treatment of primary liver cancer.METHODS Data were collected from 164 patients with primary liver cancer who underwent MWA at our hospital between March 2019 and April 2021.Among them,79 patients(control group)received routine treatments and 85 patients(research group)received Linggui Zhugan decoction in addition to routine treatment.The clinical efficacy,incidence of adverse reactions,and levels of serum alphafetoprotein(AFP),des-γ-carboxy prothrombin(DCP),AFP-L3,total bilirubin(TBil),alanine aminotransferase(ALT),CD4 cell count,CD8 cell count,and CD4/CD8 ratio were compared between the two groups,before and after treatment.The three-year recurrence rates between the two groups were compared,and independent prognostic factors for recurrence were identified.RESULTS The study results revealed that the objective response rate(ORR)in the research group was significantly higher than that in the control group(P=0.005).After treatment,the CD4 cell count and CD4/CD8 ratio significantly increased,whereas the CD8 cell count and TBil,ALT,AFP,DCP,and AFP-L3 Levels were significantly lower in the research group than in the control group(P<0.001).The Cox regression analysis revealed that the treatment regimen(P=0.003),presence of cirrhosis(P=0.019),tumor diameter(P=0.037),Child-Pugh score(P=0.003),pretreatment AFP level(P=0.006),and AFP-L3 Level(P=0.002)were independent prognostic factors for disease-free survival.CONCLUSION The combination of Linggui Zhugan decoction with MWA significantly improved the clinical efficacy and longterm prognosis of patients with primary liver cancer.展开更多
Objective:A complex relationship exists between obstructive sleep apnea syndrome and diabetes mellitus(DM).Chronic intermittent hypoxia(CIH),which is a core pathological feature of obstructive sleep apnea syndrome,may...Objective:A complex relationship exists between obstructive sleep apnea syndrome and diabetes mellitus(DM).Chronic intermittent hypoxia(CIH),which is a core pathological feature of obstructive sleep apnea syndrome,may play an important role in the onset and development of DM-related atherosclerosis(DM-AS).This study aimed to investigate the mechanism of action of tetramethylpyrazine(TMP)in CIH-associated DM-AS.Methods:In vivo,a DM-AS mouse model was established by intraperitoneal injection of streptozotocin combined with a high-fat diet.They were exposed to CIH or normoxic conditions for 8 weeks and received different doses of TMP,rosuvastatin,toyocamycin,and purified water.Glycolipid metabolism,inflammation levels,degree of aortic AS,and expression levels of endoplasmic reticulum stress(ERS)and autophagy proteins were examined in mice.In vitro,human umbilical vein endothelial cells(HUVECs)were treated with high glucose and fat in combination with insulin to establish an insulin-resistant cell model(HUVEC-IR).After pretreatment with 4μ8C(IRE1 inhibitor)and different doses of TMP,intermittent hypoxic intervention was performed.Changes in cell morphology,proliferative activity,glucose consumption,and ability to migrate were observed,and the expression levels of ERS and autophagy proteins were detected.Results:In vivo experiments showed that CIH significantly increased blood glucose levels and Homeostasis Model Assessment of Insulin Resistance(HOMA-IR)(P<0.001 or P<0.05),low-density lipoprotein cholesterol(LDL-C)content(P<0.001),and the levels of three inflammatory factors[interleukin-1 beta(IL-1β),IL-6,and tumor necrosis factor-alpha(TNF-α)]in the mice(P<0.001)compared with those of the mice with DM alone;moreover,the aortic atherosclerotic(AS)plaque area in CIH mice was significantly enlarged(P<0.001).Western blotting results showed that the expressions of aortic IRE1α,XBP1s,Beclin1,and LC3A proteins were significantly increased in CIH mice compared with DM mice(P<0.05).After treatment with different doses of TMP,rosuvastatin calcium,and toyocamycin,serum inflammation and lipid levels and plaque area were significantly reduced in mice(P<0.001 and P<0.001,respectively).The expression levels of aortic XBP1s,Beclin1,and LC3A were reduced in TMP-and toyocamycin-treated mice(P<0.05).In the in vitro experiments,compared with normoxic cells,the cells treated with intermittent hypoxia(IH)showed a significant decrease in cell migration distance(P<0.05),a significant increase in apoptosis rate(P<0.001),a substantial increase in proliferation inhibition rate(P<0.001),a significant increase in the levels of XBP1s and LC3A proteins(P<0.05),and an increase in the number of autophagic vesicles/lysosomes,as observed under transmission electron microscopy.After treatment with different doses of TMP and 4μ8C,cell morphology was significantly restored,apoptosis rate significantly reduced(P<0.001),and XBP1s,Beclin1,and LC3A expressions significantly inhibited(P<0.05).Conclusion:CIH promoted the onset of DM-AS,whereas TMP attenuated ERS and excessive autophagy by modulating the IRE1α-XBP1 signaling pathway and inhibiting XBP1 splicing,thereby ameliorating DM-AS exacerbated by CIH.展开更多
[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided ...[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided into five groups:the blank control group(basic diet),the model group(high-energy and low-protein diet,HELPD),the low-dose group(HELPD+0.5 g ACH extract per hen,LACH),and the medium-dose group(HELPD+1 g ACH extract per hen,MACH),high dose group(HELPD+2 g ACH extract per hen,HACH).The ACH extract was administrated by drinking water for 48 d.[Results]Different doses of ACH could improve the pathological changes induced by high energy and low protein.ACH extract had no significant effect on blood routine indicators of laying hens(P>0.05).The contents of total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the model group were significant-ly higher than those in the control group(P<0.05),while the content of high density lipoprotein cholesterol(HDL-C)was significantly lower than that in the control group(P<0.05).There was no significant difference in blood lipid between LACH group and model group(P>0.05).In MACH and HACH groups,the contents of TC,TG and LDL-C were significantly lower than those in the model group(P<0.05),and the content of HDL-C was significantly higher than that in the model group(P<0.05).[Conclusions]The ACH extract can regulate theHELPD-induced dyslipidemia in laying hens.展开更多
At present, the feature extraction of protein sequences is the most basic issue to predict protein structural classes and is also the key problem to decide the quality of prediction. In order to predict protein struct...At present, the feature extraction of protein sequences is the most basic issue to predict protein structural classes and is also the key problem to decide the quality of prediction. In order to predict protein structural classes accurately, we construct a 14-dimensional feature vector based on protein secondary and super-secondary structure information to reflect the content and spatial ordering of the given protein sequences. Among the vector, seven features about -helix bundle, hairpin motifs, Rossman folds, -plaits and other super-secondary structure information are first proposed in our paper. Experiments show that our method improves overall accuracy of lower similarity datasets 1189 and 640 by 0.9% - 3.8% and 0.5% - 4.2% respectively compared with other methods and has a competitive advantage for predicting proteins in and classes.展开更多
文摘BACKGROUND Liver cancer is one of the most common malignant tumors of the digestive system,and early detection and effective treatment are crucial for improving the prognosis.Microwave ablation(MWA)has shown promising results as a local therapeutic method for liver cancer;however,further improvement of its efficacy remains a key focus of current research.AIM To evaluate the clinical efficacy of Linggui Zhugan decoction combined with MWA for the treatment of primary liver cancer.METHODS Data were collected from 164 patients with primary liver cancer who underwent MWA at our hospital between March 2019 and April 2021.Among them,79 patients(control group)received routine treatments and 85 patients(research group)received Linggui Zhugan decoction in addition to routine treatment.The clinical efficacy,incidence of adverse reactions,and levels of serum alphafetoprotein(AFP),des-γ-carboxy prothrombin(DCP),AFP-L3,total bilirubin(TBil),alanine aminotransferase(ALT),CD4 cell count,CD8 cell count,and CD4/CD8 ratio were compared between the two groups,before and after treatment.The three-year recurrence rates between the two groups were compared,and independent prognostic factors for recurrence were identified.RESULTS The study results revealed that the objective response rate(ORR)in the research group was significantly higher than that in the control group(P=0.005).After treatment,the CD4 cell count and CD4/CD8 ratio significantly increased,whereas the CD8 cell count and TBil,ALT,AFP,DCP,and AFP-L3 Levels were significantly lower in the research group than in the control group(P<0.001).The Cox regression analysis revealed that the treatment regimen(P=0.003),presence of cirrhosis(P=0.019),tumor diameter(P=0.037),Child-Pugh score(P=0.003),pretreatment AFP level(P=0.006),and AFP-L3 Level(P=0.002)were independent prognostic factors for disease-free survival.CONCLUSION The combination of Linggui Zhugan decoction with MWA significantly improved the clinical efficacy and longterm prognosis of patients with primary liver cancer.
基金the National Natural Science Foundation of China(82074264)。
文摘Objective:A complex relationship exists between obstructive sleep apnea syndrome and diabetes mellitus(DM).Chronic intermittent hypoxia(CIH),which is a core pathological feature of obstructive sleep apnea syndrome,may play an important role in the onset and development of DM-related atherosclerosis(DM-AS).This study aimed to investigate the mechanism of action of tetramethylpyrazine(TMP)in CIH-associated DM-AS.Methods:In vivo,a DM-AS mouse model was established by intraperitoneal injection of streptozotocin combined with a high-fat diet.They were exposed to CIH or normoxic conditions for 8 weeks and received different doses of TMP,rosuvastatin,toyocamycin,and purified water.Glycolipid metabolism,inflammation levels,degree of aortic AS,and expression levels of endoplasmic reticulum stress(ERS)and autophagy proteins were examined in mice.In vitro,human umbilical vein endothelial cells(HUVECs)were treated with high glucose and fat in combination with insulin to establish an insulin-resistant cell model(HUVEC-IR).After pretreatment with 4μ8C(IRE1 inhibitor)and different doses of TMP,intermittent hypoxic intervention was performed.Changes in cell morphology,proliferative activity,glucose consumption,and ability to migrate were observed,and the expression levels of ERS and autophagy proteins were detected.Results:In vivo experiments showed that CIH significantly increased blood glucose levels and Homeostasis Model Assessment of Insulin Resistance(HOMA-IR)(P<0.001 or P<0.05),low-density lipoprotein cholesterol(LDL-C)content(P<0.001),and the levels of three inflammatory factors[interleukin-1 beta(IL-1β),IL-6,and tumor necrosis factor-alpha(TNF-α)]in the mice(P<0.001)compared with those of the mice with DM alone;moreover,the aortic atherosclerotic(AS)plaque area in CIH mice was significantly enlarged(P<0.001).Western blotting results showed that the expressions of aortic IRE1α,XBP1s,Beclin1,and LC3A proteins were significantly increased in CIH mice compared with DM mice(P<0.05).After treatment with different doses of TMP,rosuvastatin calcium,and toyocamycin,serum inflammation and lipid levels and plaque area were significantly reduced in mice(P<0.001 and P<0.001,respectively).The expression levels of aortic XBP1s,Beclin1,and LC3A were reduced in TMP-and toyocamycin-treated mice(P<0.05).In the in vitro experiments,compared with normoxic cells,the cells treated with intermittent hypoxia(IH)showed a significant decrease in cell migration distance(P<0.05),a significant increase in apoptosis rate(P<0.001),a substantial increase in proliferation inhibition rate(P<0.001),a significant increase in the levels of XBP1s and LC3A proteins(P<0.05),and an increase in the number of autophagic vesicles/lysosomes,as observed under transmission electron microscopy.After treatment with different doses of TMP and 4μ8C,cell morphology was significantly restored,apoptosis rate significantly reduced(P<0.001),and XBP1s,Beclin1,and LC3A expressions significantly inhibited(P<0.05).Conclusion:CIH promoted the onset of DM-AS,whereas TMP attenuated ERS and excessive autophagy by modulating the IRE1α-XBP1 signaling pathway and inhibiting XBP1 splicing,thereby ameliorating DM-AS exacerbated by CIH.
基金Supported by Special Project of Technological Innovation of Hainan Provincial Research Institute(SQKY2022-0006)Key Task of the Institute of Animal Husbandry and Veterinary of Hainan Agricultural Academy(HNXM2024ZD01).
文摘[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided into five groups:the blank control group(basic diet),the model group(high-energy and low-protein diet,HELPD),the low-dose group(HELPD+0.5 g ACH extract per hen,LACH),and the medium-dose group(HELPD+1 g ACH extract per hen,MACH),high dose group(HELPD+2 g ACH extract per hen,HACH).The ACH extract was administrated by drinking water for 48 d.[Results]Different doses of ACH could improve the pathological changes induced by high energy and low protein.ACH extract had no significant effect on blood routine indicators of laying hens(P>0.05).The contents of total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the model group were significant-ly higher than those in the control group(P<0.05),while the content of high density lipoprotein cholesterol(HDL-C)was significantly lower than that in the control group(P<0.05).There was no significant difference in blood lipid between LACH group and model group(P>0.05).In MACH and HACH groups,the contents of TC,TG and LDL-C were significantly lower than those in the model group(P<0.05),and the content of HDL-C was significantly higher than that in the model group(P<0.05).[Conclusions]The ACH extract can regulate theHELPD-induced dyslipidemia in laying hens.
文摘At present, the feature extraction of protein sequences is the most basic issue to predict protein structural classes and is also the key problem to decide the quality of prediction. In order to predict protein structural classes accurately, we construct a 14-dimensional feature vector based on protein secondary and super-secondary structure information to reflect the content and spatial ordering of the given protein sequences. Among the vector, seven features about -helix bundle, hairpin motifs, Rossman folds, -plaits and other super-secondary structure information are first proposed in our paper. Experiments show that our method improves overall accuracy of lower similarity datasets 1189 and 640 by 0.9% - 3.8% and 0.5% - 4.2% respectively compared with other methods and has a competitive advantage for predicting proteins in and classes.
