We report a low-noise, high-signal-to-noise-ratio(SNR) balanced homodyne detector based on the standard transimpedance amplifier circuit and the inductance and capacitance combination for the measurement of the bright...We report a low-noise, high-signal-to-noise-ratio(SNR) balanced homodyne detector based on the standard transimpedance amplifier circuit and the inductance and capacitance combination for the measurement of the bright squeezed state in the range from 1 kHz to 100 kHz. A capacitance is mounted at the input end of the AC branch to prevent the DC photocurrent from entering the AC branch and avoid AC branch saturation. By adding a switch at the DC branch,the DC branch can be flexibly turned on and off on different occasions. When the switch is on, the DC output provides a monitor signal for laser beam alignment. When the switch is off, the electronic noise of the AC branch is greatly reduced at audio-frequency band due to immunity to the impedance of the DC branch, hence the SNR of the AC branch is significantly improved. As a result, the electronic noise of the AC branch is close to -125 dBm, and the maximum SNR of the AC branch is 48 dB with the incident power of 8 mW in the range from 1 kHz to 100 kHz. The developed photodetector paves a path for measuring the bright squeezed state at audio-frequency band.展开更多
Objectives：To investigate the effects of adenovirus-mediated inducible nitric oxide synthase gene transfection on bladder transitional cell carcinoma T24 cells,and to provide novel insights and approaches to clinical...Objectives：To investigate the effects of adenovirus-mediated inducible nitric oxide synthase gene transfection on bladder transitional cell carcinoma T24 cells,and to provide novel insights and approaches to clinical therapies against bladder transitional cell carcinoma.Methods：Firstly,construct recombinant adenovirus vector pAd-iNOS of iNOS,followed by transfection of pAd-iNOS into HECK293 packaging cells.Thirdly,harvest recombinant adenovirus rAd-iNOS after amplification and purification procedures.Finally,transfect the recombinant adenovirus rAd-iNOS into human bladder carcinoma T24 cells and examine the effect of rAd-iNOS transfection on apoptosis of T24 and possible mechanism.Results：As shown by this study,the recombinant adenovirus rAd-iNOS was constructed successfully.The virus titer was 5.8×108 PFU/mL and recombinant was verified by PCR analysis.Transfection of adenovirus rAd-iNOS into T24 cells could induce secretion of high NO concentration,P53 protein expression upregulation,as well as promotion ofT24 cell apoptosis.Conclusions：The transfection of human bladder carcinoma T24 cells from recombinant adenovirus rAdiNOS was confirmed to induce intracellular iNOS over-expression,high production of NO,up-regulation of intracellular P53 expression and promotion of cell apoptosis.展开更多
We design and construct a resonant photodetector(RPD)with a Q factor of 320.83 at the resonant frequency of38.5 MHz on the basis of a theoretical analysis.Compared with the existing RPD under the same conditions,the s...We design and construct a resonant photodetector(RPD)with a Q factor of 320.83 at the resonant frequency of38.5 MHz on the basis of a theoretical analysis.Compared with the existing RPD under the same conditions,the signalto-noise-ratio of the error signal is increased by 15 dB and the minimum operation power is reduced from-55 dBm to-70 dBm.By comparing the standard deviations of the stability curves,we confirm that the RPD has a dramatic improvement on ultralow power extraction.In virtue of the RPD,we have completed the demonstration of channel multiplexing quantum communication.展开更多
We previously found that oxygen-glucose-serum deprivation/restoration（OGSD/R） induces apoptosis of spinal cord astrocytes, possibly via caspase-12 and the integrated stress response, which involves protein kinase R-...We previously found that oxygen-glucose-serum deprivation/restoration（OGSD/R） induces apoptosis of spinal cord astrocytes, possibly via caspase-12 and the integrated stress response, which involves protein kinase R-like endoplasmic reticulum kinase（PERK）, eukaryotic initiation factor 2-alpha（eIF2α） and activating transcription factor 4（ATF4）. We hypothesized that edaravone, a low molecular weight, lipophilic free radical scavenger, would reduce OGSD/R-induced apoptosis of spinal cord astrocytes. To test this, we established primary cultures of rat astrocytes, and exposed them to 8 hours/6 hours of OGSD/R with or without edaravone（0.1, 1, 10, 100 μM） treatment. We found that 100 μM of edaravone significantly suppressed astrocyte apoptosis and inhibited the release of reactive oxygen species. It also inhibited the activation of caspase-12 and caspase-3, and reduced the expression of homologous CCAAT/enhancer binding protein, phosphorylated（p）-PERK, p-eIF2α, and ATF4. These results point to a new use of an established drug in the prevention of OGSD/R-mediated spinal cord astrocyte apoptosis via the integrated stress response.展开更多
Using a unique adhesive locomotion system,the rock-climbing fish(Beaufortia kweichowensis)adheres to submerged surfaces and crawls both forwards and backwards in torrential streams.To emulate this mechanism,we present...Using a unique adhesive locomotion system,the rock-climbing fish(Beaufortia kweichowensis)adheres to submerged surfaces and crawls both forwards and backwards in torrential streams.To emulate this mechanism,we present a biomimetic robot inspired by the locomotion model of the rock-climbing fish.The prototype contains two anisotropic adhesive components with linkages connected to a linear actuator.Each anisotropic adhesive component consists of one commercial sucker and two retractable bioinspired fin components.The fin components mimic the abduction and adduction of pectoral and pelvic fins through the retractable part to move up and down.The robot prototype was tested on vertical and inverted surfaces,and worked successfully.These results demonstrate that this novel system represents a new locomotion solution for surface movement without detachment from the substrate.展开更多
Wheat （Triticum aestivum L.） yellow mosaic virus （WYMV） is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted pl...Wheat （Triticum aestivum L.） yellow mosaic virus （WYMV） is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted plants. In the present study, the amplified fragment length polymorphisms （AFLP） and simple sequence repeat （SSR） were used to identify the molecular linkages with the resistance gene against WYMV. Bulked segregant analysis was performed with an F2 population derived from the cross of cultivar Ningmai 9 （resistant） × cultivar Yangmai 10 （susceptible）. By screening among the resistant or susceptible parents, the F2 pools and the individuals in the F2 population with 64 combined selective AFLP primers （EcoRI/MseI） or 290 reported SSR primers, a polymorphic DNA segment （approximately 120 bp） was amplified using the primer pair E2/M5, and an SSR marker （approximately 180 bp） was located on wheat chromosome 2A using the primer Xgwm328. Analysis with MAPMAKER/Exp Version 3.0b （Whitehead institute for Biomedical Research, Cambridge, MA, USA） indicated that these two markers were dominantly associated with the resistance gene at distances of 5.4 cM or 17.6 cM, respectively. The resistance gene to WYMV derived from Ningmai 9, is temporarily named YmNM, and was mapped to wheat chromosome 2A.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11654002,61575114,11874250,and 11804207)the National Key Research and Development Program of China(Grant No.2016YFA0301401)+3 种基金the Program for Sanjin Scholar of Shanxi Province,Chinathe Program for Outstanding Innovative Teams of Higher Learning Institutions of Shanxi,Chinathe Fund for Shanxi“1331 Project”Key Subjects Construction,ChinaKey Research and Development Projects of Shanxi Province,China(Grant No.201903D111001)。
文摘We report a low-noise, high-signal-to-noise-ratio(SNR) balanced homodyne detector based on the standard transimpedance amplifier circuit and the inductance and capacitance combination for the measurement of the bright squeezed state in the range from 1 kHz to 100 kHz. A capacitance is mounted at the input end of the AC branch to prevent the DC photocurrent from entering the AC branch and avoid AC branch saturation. By adding a switch at the DC branch,the DC branch can be flexibly turned on and off on different occasions. When the switch is on, the DC output provides a monitor signal for laser beam alignment. When the switch is off, the electronic noise of the AC branch is greatly reduced at audio-frequency band due to immunity to the impedance of the DC branch, hence the SNR of the AC branch is significantly improved. As a result, the electronic noise of the AC branch is close to -125 dBm, and the maximum SNR of the AC branch is 48 dB with the incident power of 8 mW in the range from 1 kHz to 100 kHz. The developed photodetector paves a path for measuring the bright squeezed state at audio-frequency band.
基金No.B2010033 Scientific Research Foundation,Health Department,Hunan Province
文摘Objectives：To investigate the effects of adenovirus-mediated inducible nitric oxide synthase gene transfection on bladder transitional cell carcinoma T24 cells,and to provide novel insights and approaches to clinical therapies against bladder transitional cell carcinoma.Methods：Firstly,construct recombinant adenovirus vector pAd-iNOS of iNOS,followed by transfection of pAd-iNOS into HECK293 packaging cells.Thirdly,harvest recombinant adenovirus rAd-iNOS after amplification and purification procedures.Finally,transfect the recombinant adenovirus rAd-iNOS into human bladder carcinoma T24 cells and examine the effect of rAd-iNOS transfection on apoptosis of T24 and possible mechanism.Results：As shown by this study,the recombinant adenovirus rAd-iNOS was constructed successfully.The virus titer was 5.8×108 PFU/mL and recombinant was verified by PCR analysis.Transfection of adenovirus rAd-iNOS into T24 cells could induce secretion of high NO concentration,P53 protein expression upregulation,as well as promotion ofT24 cell apoptosis.Conclusions：The transfection of human bladder carcinoma T24 cells from recombinant adenovirus rAdiNOS was confirmed to induce intracellular iNOS over-expression,high production of NO,up-regulation of intracellular P53 expression and promotion of cell apoptosis.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.62027821,11654002,11874250,and 11804207)the National Key R&D Program of China(Grant No.2016YFA0301401)+3 种基金the Key R&D Program of Shanxi,China(Grant No.201903D111001)the Program for Sanjin Scholar of Shanxi Provincethe Program for Outstanding Innovative Teams of Higher Learning Institutions of Shanxi,Chinathe Fund for Shanxi“1331 Project”Key Subjects Construction,China。
文摘We design and construct a resonant photodetector(RPD)with a Q factor of 320.83 at the resonant frequency of38.5 MHz on the basis of a theoretical analysis.Compared with the existing RPD under the same conditions,the signalto-noise-ratio of the error signal is increased by 15 dB and the minimum operation power is reduced from-55 dBm to-70 dBm.By comparing the standard deviations of the stability curves,we confirm that the RPD has a dramatic improvement on ultralow power extraction.In virtue of the RPD,we have completed the demonstration of channel multiplexing quantum communication.
基金supported by a grant from the Science&Technology Bureau of Changzhou City of China,No.CJ20130029
文摘We previously found that oxygen-glucose-serum deprivation/restoration（OGSD/R） induces apoptosis of spinal cord astrocytes, possibly via caspase-12 and the integrated stress response, which involves protein kinase R-like endoplasmic reticulum kinase（PERK）, eukaryotic initiation factor 2-alpha（eIF2α） and activating transcription factor 4（ATF4）. We hypothesized that edaravone, a low molecular weight, lipophilic free radical scavenger, would reduce OGSD/R-induced apoptosis of spinal cord astrocytes. To test this, we established primary cultures of rat astrocytes, and exposed them to 8 hours/6 hours of OGSD/R with or without edaravone（0.1, 1, 10, 100 μM） treatment. We found that 100 μM of edaravone significantly suppressed astrocyte apoptosis and inhibited the release of reactive oxygen species. It also inhibited the activation of caspase-12 and caspase-3, and reduced the expression of homologous CCAAT/enhancer binding protein, phosphorylated（p）-PERK, p-eIF2α, and ATF4. These results point to a new use of an established drug in the prevention of OGSD/R-mediated spinal cord astrocyte apoptosis via the integrated stress response.
基金This work is supported by the China Postdoctoral Science Foundation(No.2020M681843)the National Natural Science Foundation of China(Nos.51875507,51821093,and 51890885)+1 种基金the Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars(No.LR15E050001)the Zhejiang Provincial Natural Science Foundation of China(No.LY18E050003).
文摘Using a unique adhesive locomotion system,the rock-climbing fish(Beaufortia kweichowensis)adheres to submerged surfaces and crawls both forwards and backwards in torrential streams.To emulate this mechanism,we present a biomimetic robot inspired by the locomotion model of the rock-climbing fish.The prototype contains two anisotropic adhesive components with linkages connected to a linear actuator.Each anisotropic adhesive component consists of one commercial sucker and two retractable bioinspired fin components.The fin components mimic the abduction and adduction of pectoral and pelvic fins through the retractable part to move up and down.The robot prototype was tested on vertical and inverted surfaces,and worked successfully.These results demonstrate that this novel system represents a new locomotion solution for surface movement without detachment from the substrate.
文摘Wheat （Triticum aestivum L.） yellow mosaic virus （WYMV） is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted plants. In the present study, the amplified fragment length polymorphisms （AFLP） and simple sequence repeat （SSR） were used to identify the molecular linkages with the resistance gene against WYMV. Bulked segregant analysis was performed with an F2 population derived from the cross of cultivar Ningmai 9 （resistant） × cultivar Yangmai 10 （susceptible）. By screening among the resistant or susceptible parents, the F2 pools and the individuals in the F2 population with 64 combined selective AFLP primers （EcoRI/MseI） or 290 reported SSR primers, a polymorphic DNA segment （approximately 120 bp） was amplified using the primer pair E2/M5, and an SSR marker （approximately 180 bp） was located on wheat chromosome 2A using the primer Xgwm328. Analysis with MAPMAKER/Exp Version 3.0b （Whitehead institute for Biomedical Research, Cambridge, MA, USA） indicated that these two markers were dominantly associated with the resistance gene at distances of 5.4 cM or 17.6 cM, respectively. The resistance gene to WYMV derived from Ningmai 9, is temporarily named YmNM, and was mapped to wheat chromosome 2A.
