Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight ...Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight junctions.Since renal pathophysiology is associated with gut barrier integrity,we hypothesized that A.muciniphila may have preventive effects on AKI.We established a lipopolysaccharide(LPS)-induced AKI mouse model to evaluate the effects of A.muciniphila.Our findings showed that pretreatment with A.muciniphila significantly attenuated kidney injury,as evidenced by reduced serum creatinine and urea nitrogen levels,alongside decreased tubular necrosis and apoptosis.A.muciniphila preserved intestinal barrier integrity and induced marked shifts in gut microbial ecology and the metabolome.A.muciniphila notably induced an increase in the relative abundance of the phylum Proteobacteria while decreasing in that of the phylum Bacteroidetes.At the genus level,Prevotella,Faecalibaculum,Moraxella,and Lactobacillus were more abundant in A.muciniphilapretreated mice.Metabolomic analysis revealed that A.muciniphila altered the gut metabolome,with changes involving pathways such as tyrosine metabolism,alanine/aspartate/glutamate homeostasis,cancer-related carbon flux,and GABAergic synaptic signaling.In conclusion,our findings indicate that A.muciniphila exerts renoprotective effects by modulating the gut-kidney axis,thereby establishing a foundation for future studies to explore the connection between gut microbiota and AKI.展开更多
In this study,we examine the problem of sliced inverse regression(SIR),a widely used method for sufficient dimension reduction(SDR).It was designed to find reduced-dimensional versions of multivariate predictors by re...In this study,we examine the problem of sliced inverse regression(SIR),a widely used method for sufficient dimension reduction(SDR).It was designed to find reduced-dimensional versions of multivariate predictors by replacing them with a minimally adequate collection of their linear combinations without loss of information.Recently,regularization methods have been proposed in SIR to incorporate a sparse structure of predictors for better interpretability.However,existing methods consider convex relaxation to bypass the sparsity constraint,which may not lead to the best subset,and particularly tends to include irrelevant variables when predictors are correlated.In this study,we approach sparse SIR as a nonconvex optimization problem and directly tackle the sparsity constraint by establishing the optimal conditions and iteratively solving them by means of the splicing technique.Without employing convex relaxation on the sparsity constraint and the orthogonal constraint,our algorithm exhibits superior empirical merits,as evidenced by extensive numerical studies.Computationally,our algorithm is much faster than the relaxed approach for the natural sparse SIR estimator.Statistically,our algorithm surpasses existing methods in terms of accuracy for central subspace estimation and best subset selection and sustains high performance even with correlated predictors.展开更多
Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii), possessing significant aesthetic value and playing a crucial role in the species' reproduction and fitness. Despit...Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii), possessing significant aesthetic value and playing a crucial role in the species' reproduction and fitness. Despite years of research, deciphering the molecular mechanisms underlying blotch formation remains challenging. As is well known, floral pigmentation is frequently associated with the familiar R2R3-MYB transcription factors. The key MYB anthocyanin activators of P. rockii ‘Shu Sheng Peng Mo' were previously reported in our preceding study. In this study, we identified and characterized three R2R3-MYBs, Pr MYBi1, Pr MYBi2, and Pr MYBi3, which belong to subgroup 4(SG4) and play repressor roles in anthocyanin biosynthesis. A quantitative real-time PCR(q RT-PCR) assay indicated that the expression of Pr MYBi1 and Pr MYBi3 gradually increased during flowering development and was substantially up-regulated in non-blotch compared to blotch. Yeast one-hybrid and dualluciferase assays demonstrated that Pr MYBi(1-3) directly target the anthocyanin structural genes and repress their transcription. The genetic transformation of tobacco demonstrated that the overexpression of Pr MYBi(1-3) decreased anthocyanin accumulation in flowers, with Pr MYBi1 serving as the most effective repressor. Our results revealed that SG4 R2R3-MYBs negatively regulate the anthocyanin pathway in P.rockii conservatively, and we provide the definite members. These findings will advance future research to unravel the mystery of blotch pattern formation.展开更多
Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transport...Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transportation capabilities,allowing them tomove in the opposite directionwithout turning around,which helps reduce transportation time.This paper aims at the problem of AGV scheduling and bidirectional conflict-free routing with battery swapping in automated terminals.A bi-level mixed integer programming(MIP)model is proposed,taking into account task assignment,bidirectional conflict-free routing,and battery swapping.The upper model focuses on container task assignment and AGV battery swapping planning,while the lower model ensures conflict-free movement of AGVs.A double-threshold battery swapping strategy is introduced,allowing AGVs to utilize waiting time for loading for battery swapping.An improved differential evolution variable neighborhood search(IDE-VNS)algorithm is developed to solve the bi-level MIP model,aiming to minimize the completion time of all jobs.Experimental results demonstrate that compared to the differential evolution(DE)algorithm and the genetic algorithm(GA),the IDEVNS algorithmreduces fitness values by 44.49% and 45.22%,though it does increase computation time by 56.28% and 62.03%,respectively.Bidirectional transportation reduces the fitness value by an average of 10.97% when the container scale is small.As the container scale increases,the fitness value of bidirectional transportation gradually approaches that of unidirectional transportation.The results further show that the double-threshold battery swapping strategy enhances AGV utilization and reduces the fitness value.展开更多
目的探讨保留直肠盲端的腹腔镜辅助肛门成形术(LAARP)治疗高位先天性肛门直肠畸形(ARM)的学龄期疗效。方法回顾性分析2013年1月至2020年11月在合肥医科大学附属医院小儿外科诊断的87例高位ARM。按治疗方式不同分为完整保留直肠组(n=51)...目的探讨保留直肠盲端的腹腔镜辅助肛门成形术(LAARP)治疗高位先天性肛门直肠畸形(ARM)的学龄期疗效。方法回顾性分析2013年1月至2020年11月在合肥医科大学附属医院小儿外科诊断的87例高位ARM。按治疗方式不同分为完整保留直肠组(n=51)和切除直肠组(n=36)。根据Rintala评分评价每组患儿学龄期的排便功能;对每组患儿进行肛门直肠测压及肛管内超声检查,统计术后肛管静息压,肛管收缩压,收缩高压带长度,直肠肛门抑制反射(RAIR),肛门内括约肌(IAS)及肛门外括约肌(EAS)的最大厚度;对排便功能障碍患儿实施肠道管理策略治疗并评估其疗效。结果保留直肠组的Rintala评分中便意、排便频次、污粪、大便失禁、社会问题均高于切除直肠组,差异均有统计学意义(P<0.05);便秘评分低于切除直肠组,差异具有统计学意义(P<0.05);保留直肠组控便能力、Rintala评分总分、Rintala评分优良率与切除直肠组比较,差异均无统计学意义(P>0.05);保留直肠组IAS最大厚度(2.73±0.59 VS 2.21±0.37)mm、EAS最大厚度(5.90±1.36 VS 5.49±10.89)mm、肛管静息压(38.56±8.45 VS 27.85±5.27)mmHg、收缩高压带长度(3.28±0.53 VS 3.00±0.45)cm均高于切除直肠组,差异均有统计学意义(P<0.05);保留直肠组肛管收缩压(82.37±16.24 VS 80.62±13.03)mmHg、RAIR阳性率(27.5%VS 13.9%)比较,差异均无统计学意义(P>0.05);87例患儿排便功能障碍发生率为39.1%(34/87),经肠道管理策略治疗后1年随访排便功能障碍发生率11.5%(10/87)。结论保留直肠的LAARP在学龄期高位ARM中能获得较好的排便功能;规范的肠道管理策略可改善ARM患儿的术后排便功能,提高远期生活质量。展开更多
基金funded by the National Natural Science Foundation of China(Grant No.82470766 to H.M.)the Jiangsu Provincial Medical Key Discipline(Laboratory)Cultivation Unit(Grant No.JSDW202206 to C.X.)the First Affiliated Hospital of Nanjing Medical University Clinical Capacity Enhancement Project(Grant No.JSPH-MC-2022-18 to C.X.).
文摘Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight junctions.Since renal pathophysiology is associated with gut barrier integrity,we hypothesized that A.muciniphila may have preventive effects on AKI.We established a lipopolysaccharide(LPS)-induced AKI mouse model to evaluate the effects of A.muciniphila.Our findings showed that pretreatment with A.muciniphila significantly attenuated kidney injury,as evidenced by reduced serum creatinine and urea nitrogen levels,alongside decreased tubular necrosis and apoptosis.A.muciniphila preserved intestinal barrier integrity and induced marked shifts in gut microbial ecology and the metabolome.A.muciniphila notably induced an increase in the relative abundance of the phylum Proteobacteria while decreasing in that of the phylum Bacteroidetes.At the genus level,Prevotella,Faecalibaculum,Moraxella,and Lactobacillus were more abundant in A.muciniphilapretreated mice.Metabolomic analysis revealed that A.muciniphila altered the gut metabolome,with changes involving pathways such as tyrosine metabolism,alanine/aspartate/glutamate homeostasis,cancer-related carbon flux,and GABAergic synaptic signaling.In conclusion,our findings indicate that A.muciniphila exerts renoprotective effects by modulating the gut-kidney axis,thereby establishing a foundation for future studies to explore the connection between gut microbiota and AKI.
文摘In this study,we examine the problem of sliced inverse regression(SIR),a widely used method for sufficient dimension reduction(SDR).It was designed to find reduced-dimensional versions of multivariate predictors by replacing them with a minimally adequate collection of their linear combinations without loss of information.Recently,regularization methods have been proposed in SIR to incorporate a sparse structure of predictors for better interpretability.However,existing methods consider convex relaxation to bypass the sparsity constraint,which may not lead to the best subset,and particularly tends to include irrelevant variables when predictors are correlated.In this study,we approach sparse SIR as a nonconvex optimization problem and directly tackle the sparsity constraint by establishing the optimal conditions and iteratively solving them by means of the splicing technique.Without employing convex relaxation on the sparsity constraint and the orthogonal constraint,our algorithm exhibits superior empirical merits,as evidenced by extensive numerical studies.Computationally,our algorithm is much faster than the relaxed approach for the natural sparse SIR estimator.Statistically,our algorithm surpasses existing methods in terms of accuracy for central subspace estimation and best subset selection and sustains high performance even with correlated predictors.
基金supported by the National Natural Science Foundation of China(Grant No.32030095)the Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302).
文摘Petal blotch is a prevalent pigmentation pattern observed in the Xibei tree peony(Paeonia rockii), possessing significant aesthetic value and playing a crucial role in the species' reproduction and fitness. Despite years of research, deciphering the molecular mechanisms underlying blotch formation remains challenging. As is well known, floral pigmentation is frequently associated with the familiar R2R3-MYB transcription factors. The key MYB anthocyanin activators of P. rockii ‘Shu Sheng Peng Mo' were previously reported in our preceding study. In this study, we identified and characterized three R2R3-MYBs, Pr MYBi1, Pr MYBi2, and Pr MYBi3, which belong to subgroup 4(SG4) and play repressor roles in anthocyanin biosynthesis. A quantitative real-time PCR(q RT-PCR) assay indicated that the expression of Pr MYBi1 and Pr MYBi3 gradually increased during flowering development and was substantially up-regulated in non-blotch compared to blotch. Yeast one-hybrid and dualluciferase assays demonstrated that Pr MYBi(1-3) directly target the anthocyanin structural genes and repress their transcription. The genetic transformation of tobacco demonstrated that the overexpression of Pr MYBi(1-3) decreased anthocyanin accumulation in flowers, with Pr MYBi1 serving as the most effective repressor. Our results revealed that SG4 R2R3-MYBs negatively regulate the anthocyanin pathway in P.rockii conservatively, and we provide the definite members. These findings will advance future research to unravel the mystery of blotch pattern formation.
基金supported by National Natural Science Foundation of China(No.62073212)Shanghai Science and Technology Commission(No.23ZR1426600).
文摘Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transportation capabilities,allowing them tomove in the opposite directionwithout turning around,which helps reduce transportation time.This paper aims at the problem of AGV scheduling and bidirectional conflict-free routing with battery swapping in automated terminals.A bi-level mixed integer programming(MIP)model is proposed,taking into account task assignment,bidirectional conflict-free routing,and battery swapping.The upper model focuses on container task assignment and AGV battery swapping planning,while the lower model ensures conflict-free movement of AGVs.A double-threshold battery swapping strategy is introduced,allowing AGVs to utilize waiting time for loading for battery swapping.An improved differential evolution variable neighborhood search(IDE-VNS)algorithm is developed to solve the bi-level MIP model,aiming to minimize the completion time of all jobs.Experimental results demonstrate that compared to the differential evolution(DE)algorithm and the genetic algorithm(GA),the IDEVNS algorithmreduces fitness values by 44.49% and 45.22%,though it does increase computation time by 56.28% and 62.03%,respectively.Bidirectional transportation reduces the fitness value by an average of 10.97% when the container scale is small.As the container scale increases,the fitness value of bidirectional transportation gradually approaches that of unidirectional transportation.The results further show that the double-threshold battery swapping strategy enhances AGV utilization and reduces the fitness value.
文摘目的探讨保留直肠盲端的腹腔镜辅助肛门成形术(LAARP)治疗高位先天性肛门直肠畸形(ARM)的学龄期疗效。方法回顾性分析2013年1月至2020年11月在合肥医科大学附属医院小儿外科诊断的87例高位ARM。按治疗方式不同分为完整保留直肠组(n=51)和切除直肠组(n=36)。根据Rintala评分评价每组患儿学龄期的排便功能;对每组患儿进行肛门直肠测压及肛管内超声检查,统计术后肛管静息压,肛管收缩压,收缩高压带长度,直肠肛门抑制反射(RAIR),肛门内括约肌(IAS)及肛门外括约肌(EAS)的最大厚度;对排便功能障碍患儿实施肠道管理策略治疗并评估其疗效。结果保留直肠组的Rintala评分中便意、排便频次、污粪、大便失禁、社会问题均高于切除直肠组,差异均有统计学意义(P<0.05);便秘评分低于切除直肠组,差异具有统计学意义(P<0.05);保留直肠组控便能力、Rintala评分总分、Rintala评分优良率与切除直肠组比较,差异均无统计学意义(P>0.05);保留直肠组IAS最大厚度(2.73±0.59 VS 2.21±0.37)mm、EAS最大厚度(5.90±1.36 VS 5.49±10.89)mm、肛管静息压(38.56±8.45 VS 27.85±5.27)mmHg、收缩高压带长度(3.28±0.53 VS 3.00±0.45)cm均高于切除直肠组,差异均有统计学意义(P<0.05);保留直肠组肛管收缩压(82.37±16.24 VS 80.62±13.03)mmHg、RAIR阳性率(27.5%VS 13.9%)比较,差异均无统计学意义(P>0.05);87例患儿排便功能障碍发生率为39.1%(34/87),经肠道管理策略治疗后1年随访排便功能障碍发生率11.5%(10/87)。结论保留直肠的LAARP在学龄期高位ARM中能获得较好的排便功能;规范的肠道管理策略可改善ARM患儿的术后排便功能,提高远期生活质量。
