Shield tail grouting is an important measure to control tunnelling-induced ground deformation by injecting prepared grouting mate-rials to fill the tail gap.The working performance of grout is usually invisible and ha...Shield tail grouting is an important measure to control tunnelling-induced ground deformation by injecting prepared grouting mate-rials to fill the tail gap.The working performance of grout is usually invisible and hard to obtain in construction.This paper carries out an experimental study to investigate the tail grout behavior in ground.In the current research,a testing device is developed to explore the grout behavior in varying soils.The grout working performance is evaluated not only by the liquid grout properties such as fluidity,consistency,bleeding rate,stone rate and compressed deformation but also solid grout properties such as unconfined compressive strength and permeability.Three typical grouts are chosen and their behaviors in the various soils are observed.To take an insight on the behaviors,scanning electron microscopy and mercury intrusion porosimetry analysis are employed.The microstructure of solid grout is a sign of its working performance.The observation shows that the solid grout micro-structure is influenced by grout proportions,pressure,and ground permeabilities.The experimental results are applied in the case of Beijing Metro Line 12 for validation and as a result,the ground movement is inhibited due to high performance of tail grout.展开更多
Damage to the epithelial barrier is among key processes contributing to initiation and chronic inflammation in inflammatory bowel diseases(IBD).Only management therapy exists for IBD(e.g.,anti-inflammatory and immunom...Damage to the epithelial barrier is among key processes contributing to initiation and chronic inflammation in inflammatory bowel diseases(IBD).Only management therapy exists for IBD(e.g.,anti-inflammatory and immunomodulatory agents,JAK/STAT inhibitors),and while novel therapeutic approaches have shown great potential,issues remain including route of administration,development of resistance to therapy and toxicity.Thus,novel small molecule inhibitors which can alleviate colonic inflammation and restore intestinal barrier functions are needed.Our previous study identified a new quinazolinone derivative MR2938,inspired by marine natural product penipanoid C,displaying impressive anti-inflammatory effects.In vivo efficacy study indicated that MR2938 had a dose-dependent effect on improving colitis symptoms,gut-barrier disruption,and colonic inflammation in an acute dextran sulfate sodium(DSS)-induced murine colitis as a model of epi-thelial injury relevant to IBD.Evaluation of potential mechanism involved in MR2938 efficacy demonstrated that MR2938 inhibited NF-κB-mediated inflammatory responses,and attenuated intestinal epithelial tight junction damage by restoring the expression of Occludin and ZO-1.Taken together,these data suggest that MR2938 is a promising lead compound for the treatment of IBD.展开更多
The gingiva is a key oral barrier that protects oral tissues from various stimuli.A loss of gingival tissue homeostasis causes periodontitis,one of the most prevalent inflammatory diseases in humans.The human gingiva ...The gingiva is a key oral barrier that protects oral tissues from various stimuli.A loss of gingival tissue homeostasis causes periodontitis,one of the most prevalent inflammatory diseases in humans.The human gingiva exists as a complex cell network comprising specialized structures.To understand the tissue-specific pathophysiology of the gingiva,we applied a recently developed spatial enhanced resolution omics-sequencing(Stereo-seq)technique to obtain a spatial transcriptome(ST)atlas of the gingiva in healthy individuals and periodontitis patients.By utilizing Stereo-seq,we identified the major cell types present in the gingiva,which included epithelial cells,fibroblasts,endothelial cells,and immune cells,as well as subgroups of epithelial cells and immune cells.We further observed that inflammation-related signalling pathways,such as the JAK-STAT and NF-κB signalling pathways,were significantly upregulated in the endothelial cells of the gingiva of periodontitis patients compared with those of healthy individuals.Additionally,we characterized the spatial distribution of periodontitis risk genes in the gingiva and found that the expression of IFI16 was significantly increased in endothelial cells of inflamed gingiva.In conclusion,our Stereo-seq findings may facilitate the development of innovative therapeutic strategies for periodontitis by mapping periodontitis-relevant genes and pathways and effector cells.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.51978430)Natural Science Foundation of Jiangsu Province,China(Grant No.BK20231318)+3 种基金Natural Science Foundation of Zhejiang Province,China(Grant Nos.LY21E080004 and LQ24F020039)Hangzhou City University,China(Grant No.J-202211)Open fund project of Key Laboratory of Safe Construction and Intelligent Maintenance for Urban Shield Tunnels of Zhejiang Province(Grant No.ZUCC-UST-22-12)Open Research Fund of Key Laboratory of Coastal Urban Resilient Infrastructures(Shenzhen University),Ministry of Education(Grant No.CURI-G202304).
文摘Shield tail grouting is an important measure to control tunnelling-induced ground deformation by injecting prepared grouting mate-rials to fill the tail gap.The working performance of grout is usually invisible and hard to obtain in construction.This paper carries out an experimental study to investigate the tail grout behavior in ground.In the current research,a testing device is developed to explore the grout behavior in varying soils.The grout working performance is evaluated not only by the liquid grout properties such as fluidity,consistency,bleeding rate,stone rate and compressed deformation but also solid grout properties such as unconfined compressive strength and permeability.Three typical grouts are chosen and their behaviors in the various soils are observed.To take an insight on the behaviors,scanning electron microscopy and mercury intrusion porosimetry analysis are employed.The microstructure of solid grout is a sign of its working performance.The observation shows that the solid grout micro-structure is influenced by grout proportions,pressure,and ground permeabilities.The experimental results are applied in the case of Beijing Metro Line 12 for validation and as a result,the ground movement is inhibited due to high performance of tail grout.
基金supported by National Natural Science Foundation of China(Grant No.42176109,41830535)Fun-damental Research Funds for the Central Universities of China(Grant No.202241008,202212019)Shanghai Frontiers Science Center of Drug Target Identification and Delivery,School of Pharmaceutical Sciences,Shanghai Jiao Tong University,Shanghai 200240,China(Grant No.ZXWH2170101).
文摘Damage to the epithelial barrier is among key processes contributing to initiation and chronic inflammation in inflammatory bowel diseases(IBD).Only management therapy exists for IBD(e.g.,anti-inflammatory and immunomodulatory agents,JAK/STAT inhibitors),and while novel therapeutic approaches have shown great potential,issues remain including route of administration,development of resistance to therapy and toxicity.Thus,novel small molecule inhibitors which can alleviate colonic inflammation and restore intestinal barrier functions are needed.Our previous study identified a new quinazolinone derivative MR2938,inspired by marine natural product penipanoid C,displaying impressive anti-inflammatory effects.In vivo efficacy study indicated that MR2938 had a dose-dependent effect on improving colitis symptoms,gut-barrier disruption,and colonic inflammation in an acute dextran sulfate sodium(DSS)-induced murine colitis as a model of epi-thelial injury relevant to IBD.Evaluation of potential mechanism involved in MR2938 efficacy demonstrated that MR2938 inhibited NF-κB-mediated inflammatory responses,and attenuated intestinal epithelial tight junction damage by restoring the expression of Occludin and ZO-1.Taken together,these data suggest that MR2938 is a promising lead compound for the treatment of IBD.
基金supported by the National Natural Science Foundation of China(82201011,82030031,92149301 and 82270945)the Beijing Municipal Government grant(Beijing Laboratory of Oral Health,PXM2021-014226-000041)+2 种基金the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2019-I2M-5-038)the Science and Technology Project of Guangzhou,China(202206080009)the Postdoctoral Foundation of China(2021M703695 and 2021TQ0308)。
文摘The gingiva is a key oral barrier that protects oral tissues from various stimuli.A loss of gingival tissue homeostasis causes periodontitis,one of the most prevalent inflammatory diseases in humans.The human gingiva exists as a complex cell network comprising specialized structures.To understand the tissue-specific pathophysiology of the gingiva,we applied a recently developed spatial enhanced resolution omics-sequencing(Stereo-seq)technique to obtain a spatial transcriptome(ST)atlas of the gingiva in healthy individuals and periodontitis patients.By utilizing Stereo-seq,we identified the major cell types present in the gingiva,which included epithelial cells,fibroblasts,endothelial cells,and immune cells,as well as subgroups of epithelial cells and immune cells.We further observed that inflammation-related signalling pathways,such as the JAK-STAT and NF-κB signalling pathways,were significantly upregulated in the endothelial cells of the gingiva of periodontitis patients compared with those of healthy individuals.Additionally,we characterized the spatial distribution of periodontitis risk genes in the gingiva and found that the expression of IFI16 was significantly increased in endothelial cells of inflamed gingiva.In conclusion,our Stereo-seq findings may facilitate the development of innovative therapeutic strategies for periodontitis by mapping periodontitis-relevant genes and pathways and effector cells.
