Neuronal cell death is a common outcome of multiple pathophysiological processes and a key factor in neurological dysfunction after subarachnoid hemorrhage.Neuronal ferroptosis in particular plays an important role in...Neuronal cell death is a common outcome of multiple pathophysiological processes and a key factor in neurological dysfunction after subarachnoid hemorrhage.Neuronal ferroptosis in particular plays an important role in early brain injury.Bromodomain-containing protein 4,a member of the bromo and extraterminal domain family of proteins,participated in multiple cell death pathways,but the mechanisms by which it regulates ferroptosis remain unclear.The primary aim of this study was to investigate how bromodomain-containing protein 4 affects neuronal ferroptosis following subarachnoid hemorrhage in vivo and in vitro.Our findings revealed that endogenous bromodomain-containing protein 4 co-localized with neurons,and its expression was decreased 48 hours after subarachnoid hemorrhage of the cerebral cortex in vivo.In addition,ferroptosis-related pathways were activated in vivo and in vitro after subarachnoid hemorrhage.Targeted inhibition of bromodomain-containing protein 4 in neurons increased lipid peroxidation and intracellular ferrous iron accumulation via ferritinophagy and ultimately led to neuronal ferroptosis.Using cleavage under targets and tagmentation analysis,we found that bromodomain-containing protein 4 enrichment in the Raf-1 promoter region decreased following oxyhemoglobin stimulation in vitro.Furthermore,treating bromodomain-containing protein 4-knockdown HT-22 cell lines with GW5074,a Raf-1 inhibitor,exacerbated neuronal ferroptosis by suppressing the Raf-1/ERK1/2 signaling pathway.Moreover,targeted inhibition of neuronal bromodomain-containing protein 4 exacerbated early and long-term neurological function deficits after subarachnoid hemorrhage.Our findings suggest that bromodomain-containing protein 4 may have neuroprotective effects after subarachnoid hemorrhage,and that inhibiting ferroptosis could help treat subarachnoid hemorrhage.展开更多
The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.H...The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.However,maintaining consistent forwarding states during these updates is challenging,particularly when rerouting multiple flows simultaneously.Existing approaches pay little attention to multi-flow update,where improper update sequences across data plane nodes may construct deadlock dependencies.Moreover,these methods typically involve excessive control-data plane interactions,incurring significant resource overhead and performance degradation.This paper presents P4LoF,an efficient loop-free update approach that enables the controller to reroute multiple flows through minimal interactions.P4LoF first utilizes a greedy-based algorithm to generate the shortest update dependency chain for the single-flow update.These chains are then dynamically merged into a dependency graph and resolved as a Shortest Common Super-sequence(SCS)problem to produce the update sequence of multi-flow update.To address deadlock dependencies in multi-flow updates,P4LoF builds a deadlock-fix forwarding model that leverages the flexible packet processing capabilities of the programmable data plane.Experimental results show that P4LoF reduces control-data plane interactions by at least 32.6%with modest overhead,while effectively guaranteeing loop-free consistency.展开更多
Autonomic disturbances often occur in patients with acute cerebrovascular disease due to damage of the central autonomic network. We summarize the structures of the central autonomic network and the clinical tests use...Autonomic disturbances often occur in patients with acute cerebrovascular disease due to damage of the central autonomic network. We summarize the structures of the central autonomic network and the clinical tests used to evaluate the functions of the autonomic nervous system.We review the clinical and experimental findings as well as management strategies of post-stroke autonomic disturbances including electrocardiographic changes, cardiac arrhythmias, myocardial damage, thermoregulatory dysfunction, gastrointestinal dysfunction, urinary incontinence, sexual disorders, and hyperglycemia. The occurrence of autonomic disturbances has been associated with poor outcomes in stroke patients. Autonomic nervous system modulation appears to be an emerging therapeutic strategy for stroke management in addition to treatments for sensorimotor dysfunction.展开更多
This review summarizes and analyzes the basic information of various types of road transport natural disaster emergencies,refers to various types of road transport emergency plans,and combines the actual needs of road...This review summarizes and analyzes the basic information of various types of road transport natural disaster emergencies,refers to various types of road transport emergency plans,and combines the actual needs of road transport emergency rescue with national emergency related laws.It also proposes the classification criteria and grading standard for the emergencies of road transport natural disasters based on the classification and grading standard of the regulations,which provide a basis to take reasonable and effective disposal measures in the emergency response of road transport emergencies under natural disaster conditions.展开更多
We collected high-quality teleseismic events recorded by 12 broadband seismographs deployed in the Anyuan Coal Mine and its adjacent areas in Pingxiang City,Jiangxi Province for nearly two years.The H-κ-c stacking me...We collected high-quality teleseismic events recorded by 12 broadband seismographs deployed in the Anyuan Coal Mine and its adjacent areas in Pingxiang City,Jiangxi Province for nearly two years.The H-κ-c stacking method was employed to obtain the crustal thickness and Poisson's ratio distribution,then the characteristics of crustal structure below the stations were obtained by using the time-domain linear inversion method.The crustal thickness in the Anyuan Coal Mine and its adjacent areas ranges from approximately 32~35 km,with an average thickness of 33 km,which is consistent with the crustal thickness results in South China from previous studies using the receiver function method.The average Poisson's ratio of the crustal bulk composition in the study area varies between 0.22 and 0.25,which is lower than the global value with a 0.27 average,indicating a predominantly intermediate-acidic or felsic crustal composition.There is a weak negative correlation between Poisson's ratio and crustal thickness estimates in the Anyuan Coal Mine and its adjacent areas,suggesting that the absence of mafic-ultramafic materials in the lower crust is associated with the process of crustal delamination.The velocity inversion results indicate that the crustal structure including three velocity discontinuity interfaces,with the first at a depth of approximately 1.5 km,the second at about 10~15 km,and the third being the Moho.The study also indicates that the results obtained by the H-κ-c stacking method are significantly better than those obtained by the H-κmethod,effectively reducing the standard deviation and dispersion of crustal thickness and vP/vSratio.展开更多
Polyether-ether-ketone(PEEK)is believed to be the next-generation biomedical material for orthopaedic implants that may replace metal materials because of its good biocompatibility,appropriate mechanical properties an...Polyether-ether-ketone(PEEK)is believed to be the next-generation biomedical material for orthopaedic implants that may replace metal materials because of its good biocompatibility,appropriate mechanical properties and radiolucency.Currently,some PEEK implants have been used successfully for many years.However,there is no customised PEEK orthopaedic implant made by additive manufacturing licensed for the market,although clinical trials have been increasingly reported.In this review article,design criteria,including geometric matching,functional restoration,strength safety,early fixation,long-term stability and manufacturing capability,are summarised,focusing on the clinical requirements.An integrated framework of design and manufacturing processes to create customised PEEK implants is presented,and several typical clinical applications such as cranioplasty patches,rib prostheses,mandibular prostheses,scapula prostheses and femoral prostheses are described.The main technical challenge faced by PEEK orthopaedic implants lies in the poor bonding with bone and soft tissue due to its biological inertness,which may be solved by adding bioactive fillers and manufacturing porous architecture.The lack of technical standards is also one of the major factors preventing additive-manufactured customised PEEK orthopaedic implants from clinical translation,and it is good to see that the abundance of standards in the field of additive-manufactured medical devices is helping them enter the clinical market.展开更多
SARS-CoV-2 has been demonstrated to be highly susceptible to neuron-and glial-like cells,1 but there is no consensus regarding how the virus affects neurons following neuroinvasion.It is therefore unknown if and how S...SARS-CoV-2 has been demonstrated to be highly susceptible to neuron-and glial-like cells,1 but there is no consensus regarding how the virus affects neurons following neuroinvasion.It is therefore unknown if and how SARS-CoV-2 maintains the homeostasis of neuronal cells.Herein,we revealed that the receptor-binding domain(RBD)of the SARS-CoV-2 spike protein acted as an extracellular remodeling component to undermine epigenetic regulation,perturbnascentRNA(nsRNA)transcription,promote mitochondrial biogenesis,and increase cellular viability in SH-SY5Y cells.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82371310(to YJ),82271306(to JP)the Sichuan Science and Technology Support Program,Nos.2023YFH0069(to JP),2023NSFSC0028(to YJ),2023NSFSC1559(to YJ),2022YFS0615(to JP),2022NSFSC1421(to JP)+1 种基金Scientific Research Project of Sichuan Provincial Health Commission,No.23LCYJ040(to YJ)Youth Foundation of Southwestern Medical University and Southwest Medical University Project,Nos.2020ZRQNA038(to JP),2021ZKZD013(to JP),2021LZXNYD-P01(to YJ),2023QN014(to JP).
文摘Neuronal cell death is a common outcome of multiple pathophysiological processes and a key factor in neurological dysfunction after subarachnoid hemorrhage.Neuronal ferroptosis in particular plays an important role in early brain injury.Bromodomain-containing protein 4,a member of the bromo and extraterminal domain family of proteins,participated in multiple cell death pathways,but the mechanisms by which it regulates ferroptosis remain unclear.The primary aim of this study was to investigate how bromodomain-containing protein 4 affects neuronal ferroptosis following subarachnoid hemorrhage in vivo and in vitro.Our findings revealed that endogenous bromodomain-containing protein 4 co-localized with neurons,and its expression was decreased 48 hours after subarachnoid hemorrhage of the cerebral cortex in vivo.In addition,ferroptosis-related pathways were activated in vivo and in vitro after subarachnoid hemorrhage.Targeted inhibition of bromodomain-containing protein 4 in neurons increased lipid peroxidation and intracellular ferrous iron accumulation via ferritinophagy and ultimately led to neuronal ferroptosis.Using cleavage under targets and tagmentation analysis,we found that bromodomain-containing protein 4 enrichment in the Raf-1 promoter region decreased following oxyhemoglobin stimulation in vitro.Furthermore,treating bromodomain-containing protein 4-knockdown HT-22 cell lines with GW5074,a Raf-1 inhibitor,exacerbated neuronal ferroptosis by suppressing the Raf-1/ERK1/2 signaling pathway.Moreover,targeted inhibition of neuronal bromodomain-containing protein 4 exacerbated early and long-term neurological function deficits after subarachnoid hemorrhage.Our findings suggest that bromodomain-containing protein 4 may have neuroprotective effects after subarachnoid hemorrhage,and that inhibiting ferroptosis could help treat subarachnoid hemorrhage.
基金supported by the National Key Research and Development Program of China under Grant 2022YFB2901501in part by the Science and Technology Innovation leading Talents Subsidy Project of Central Plains under Grant 244200510038.
文摘The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.However,maintaining consistent forwarding states during these updates is challenging,particularly when rerouting multiple flows simultaneously.Existing approaches pay little attention to multi-flow update,where improper update sequences across data plane nodes may construct deadlock dependencies.Moreover,these methods typically involve excessive control-data plane interactions,incurring significant resource overhead and performance degradation.This paper presents P4LoF,an efficient loop-free update approach that enables the controller to reroute multiple flows through minimal interactions.P4LoF first utilizes a greedy-based algorithm to generate the shortest update dependency chain for the single-flow update.These chains are then dynamically merged into a dependency graph and resolved as a Shortest Common Super-sequence(SCS)problem to produce the update sequence of multi-flow update.To address deadlock dependencies in multi-flow updates,P4LoF builds a deadlock-fix forwarding model that leverages the flexible packet processing capabilities of the programmable data plane.Experimental results show that P4LoF reduces control-data plane interactions by at least 32.6%with modest overhead,while effectively guaranteeing loop-free consistency.
基金supported partly by grants from the National Institutes of Health,USA(NS081740 and NS082184)
文摘Autonomic disturbances often occur in patients with acute cerebrovascular disease due to damage of the central autonomic network. We summarize the structures of the central autonomic network and the clinical tests used to evaluate the functions of the autonomic nervous system.We review the clinical and experimental findings as well as management strategies of post-stroke autonomic disturbances including electrocardiographic changes, cardiac arrhythmias, myocardial damage, thermoregulatory dysfunction, gastrointestinal dysfunction, urinary incontinence, sexual disorders, and hyperglycemia. The occurrence of autonomic disturbances has been associated with poor outcomes in stroke patients. Autonomic nervous system modulation appears to be an emerging therapeutic strategy for stroke management in addition to treatments for sensorimotor dysfunction.
文摘This review summarizes and analyzes the basic information of various types of road transport natural disaster emergencies,refers to various types of road transport emergency plans,and combines the actual needs of road transport emergency rescue with national emergency related laws.It also proposes the classification criteria and grading standard for the emergencies of road transport natural disasters based on the classification and grading standard of the regulations,which provide a basis to take reasonable and effective disposal measures in the emergency response of road transport emergencies under natural disaster conditions.
基金supported by the Spark Program of Earthquake Technology of CEA,China(XH20032)open Research Project from the State Key Laboratory of Nuclear Resources and Environment,East China University of Technology(2022NRE17)+1 种基金open Research Project from the State Key Laboratory of Geological Processes,Mineral Resources,China University of Geosciences(GPMR202114)the Graduate Student Innovation Fund of East China University of Technology(YC2022-s628)。
文摘We collected high-quality teleseismic events recorded by 12 broadband seismographs deployed in the Anyuan Coal Mine and its adjacent areas in Pingxiang City,Jiangxi Province for nearly two years.The H-κ-c stacking method was employed to obtain the crustal thickness and Poisson's ratio distribution,then the characteristics of crustal structure below the stations were obtained by using the time-domain linear inversion method.The crustal thickness in the Anyuan Coal Mine and its adjacent areas ranges from approximately 32~35 km,with an average thickness of 33 km,which is consistent with the crustal thickness results in South China from previous studies using the receiver function method.The average Poisson's ratio of the crustal bulk composition in the study area varies between 0.22 and 0.25,which is lower than the global value with a 0.27 average,indicating a predominantly intermediate-acidic or felsic crustal composition.There is a weak negative correlation between Poisson's ratio and crustal thickness estimates in the Anyuan Coal Mine and its adjacent areas,suggesting that the absence of mafic-ultramafic materials in the lower crust is associated with the process of crustal delamination.The velocity inversion results indicate that the crustal structure including three velocity discontinuity interfaces,with the first at a depth of approximately 1.5 km,the second at about 10~15 km,and the third being the Moho.The study also indicates that the results obtained by the H-κ-c stacking method are significantly better than those obtained by the H-κmethod,effectively reducing the standard deviation and dispersion of crustal thickness and vP/vSratio.
基金The work was supported by the National Key R&D Program of China(No.2018YFE0207900)the National Natural Science Foundation of China(No.51835010)+6 种基金the Key R&D Program of Guangdong Province,China(No.2018B090906001)China Postdoctoral Science Foundation(No.2020M683458)the Natural Science Basic Research Program of ShaanXi Province,China(No.2022JQ-378)the Engineering and Physical Science Research Council(EPSRC)via DTP Case Programme(No.EP/T517793/1)the Fundamental Research Funds for the Central Universities(No.XZY012021007)the Laboratory Construction Project for Jihua Laboratory(No.X200031TM200),Open Project(No.BHJ17C019)the Youth Innovation Team of Shaanxi Universities.
文摘Polyether-ether-ketone(PEEK)is believed to be the next-generation biomedical material for orthopaedic implants that may replace metal materials because of its good biocompatibility,appropriate mechanical properties and radiolucency.Currently,some PEEK implants have been used successfully for many years.However,there is no customised PEEK orthopaedic implant made by additive manufacturing licensed for the market,although clinical trials have been increasingly reported.In this review article,design criteria,including geometric matching,functional restoration,strength safety,early fixation,long-term stability and manufacturing capability,are summarised,focusing on the clinical requirements.An integrated framework of design and manufacturing processes to create customised PEEK implants is presented,and several typical clinical applications such as cranioplasty patches,rib prostheses,mandibular prostheses,scapula prostheses and femoral prostheses are described.The main technical challenge faced by PEEK orthopaedic implants lies in the poor bonding with bone and soft tissue due to its biological inertness,which may be solved by adding bioactive fillers and manufacturing porous architecture.The lack of technical standards is also one of the major factors preventing additive-manufactured customised PEEK orthopaedic implants from clinical translation,and it is good to see that the abundance of standards in the field of additive-manufactured medical devices is helping them enter the clinical market.
基金supported by grants from The National Natural Science Foundation of China(No.81971132,82271306)the Sichuan Science and Technology Program(China)(No.2021ZYD0106,2021ZYD0091,22ZDYF3783,2022YFS0615,2023YFH0069,2023NSFSC0028,2023NSFSC1559)+1 种基金Project of Luzhou Science and Technology Bureau(No.2021LZXNYDP01,2021LZXNYD-Z05)Luzhou Government-Southwest Medical University Strategic Cooperation Project and Southwest Medical University Project(Sichuan,China)(No.2021ZKQN058,2020ZRQNB072,2021ZKZD013,2021ZKQN041).
文摘SARS-CoV-2 has been demonstrated to be highly susceptible to neuron-and glial-like cells,1 but there is no consensus regarding how the virus affects neurons following neuroinvasion.It is therefore unknown if and how SARS-CoV-2 maintains the homeostasis of neuronal cells.Herein,we revealed that the receptor-binding domain(RBD)of the SARS-CoV-2 spike protein acted as an extracellular remodeling component to undermine epigenetic regulation,perturbnascentRNA(nsRNA)transcription,promote mitochondrial biogenesis,and increase cellular viability in SH-SY5Y cells.
