Four new N-acylated aminoalkanoic acids,namely clonoroseins E−H(1−4),together with three previously identified analogs,clonoroseins A,B,and D(5−7),were identified from the endophytic fungus Clonostachys rosea strain 1...Four new N-acylated aminoalkanoic acids,namely clonoroseins E−H(1−4),together with three previously identified analogs,clonoroseins A,B,and D(5−7),were identified from the endophytic fungus Clonostachys rosea strain 15020(CR15020),using Feature-based Molecular Networking(FBMN).The elucidation of their chemical structures,including their absolute configurations,was achieved through spectroscopic analysis combined with quantum chemical calculations.Bioinformatics analyses suggested that an iterative type I HR-PKS(CrsE)generates the polyketide side chain of these clonoroseins.Furthermore,a downstream adenylate-forming enzyme of the PKS(CrsD)was suspected to function as an amide synthetase.CrsD potentially facilitates the transformation of the polyketide moiety into an acyl-AMP intermediate,followed by nucleophilic substitution with eitherβ-alanine orγ-aminobutyric acid to produce amide derivatives.These findings significantly expand our understanding of PKS-related products originating from C.rosea and also underscore the powerful application of FBMN analytical methods in characterization of new compounds.展开更多
The global market demand for natural astaxanthin is rapidly increasing owing to its safety,the potential health benefits,and the diverse applications in food and pharmaceutical industries.The major native producers of...The global market demand for natural astaxanthin is rapidly increasing owing to its safety,the potential health benefits,and the diverse applications in food and pharmaceutical industries.The major native producers of natural astaxanthin on industrial scale are the alga Haematococcus pluvialis and the yeast Xanthopyllomyces dendrorhous.However,the natural production via these native producers is facing challenges of limited yield and high cost of cultivation and extraction.Alternatively,astaxanthin production via metabolically engineered non-native microbial cell factories such as Escherichia coli,Saccharomyces cerevisiae and Yarrowia lipolytica is another promising strategy to overcome these limitations.In this review we summarize the recent scientific and biotechnological progresses on astaxanthin biosynthetic pathways,transcriptional regulations,the interrelation with lipid metabolism,engineering strategies as well as fermentation process control in major native and non-native astaxanthin producers.These progresses illuminate the prospects of producing astaxanthin by microbial cell factories on industrial scale.展开更多
Plant natural products have been widely used in medicine,food,and cosmetics.Of a particular note,many natural products from Cathar-anthus roseus have important biological activities and medicinal value^([1]),with vinb...Plant natural products have been widely used in medicine,food,and cosmetics.Of a particular note,many natural products from Cathar-anthus roseus have important biological activities and medicinal value^([1]),with vinblastine as one of the most effective drugs for cancer treatment^([2]).Due to difficulties in chemical synthesis of such complex structures,plant extraction is still the major method for large-scale preparation of plant natural products.展开更多
Excess amount of CO_(2) in the atmosphere is one of the major causes of the greenhouse effect and global warming[1].CO_(2),a promising feedstock for bio-refinery,its efficient utilization is challenging due to its sta...Excess amount of CO_(2) in the atmosphere is one of the major causes of the greenhouse effect and global warming[1].CO_(2),a promising feedstock for bio-refinery,its efficient utilization is challenging due to its stable structure and low energy state[2,3].However,small energetic compounds like methanol become a bridge and bond of efficient CO_(2) transformation[4,5].Therefore,methanol biotransformation via microbial cell factories will pave the way for sustainable production of value-added chemicals.展开更多
基金support from the National Key Research and Development Program of China(2019YFA0906200)the National Natural Science Foundation of China(21977029,81903529,21877038,31720103901,and 81573341)the Open Project Funding of the State Key Laboratory of Bioreactor Engineering,the 111 Project(B18022).
文摘Four new N-acylated aminoalkanoic acids,namely clonoroseins E−H(1−4),together with three previously identified analogs,clonoroseins A,B,and D(5−7),were identified from the endophytic fungus Clonostachys rosea strain 15020(CR15020),using Feature-based Molecular Networking(FBMN).The elucidation of their chemical structures,including their absolute configurations,was achieved through spectroscopic analysis combined with quantum chemical calculations.Bioinformatics analyses suggested that an iterative type I HR-PKS(CrsE)generates the polyketide side chain of these clonoroseins.Furthermore,a downstream adenylate-forming enzyme of the PKS(CrsD)was suspected to function as an amide synthetase.CrsD potentially facilitates the transformation of the polyketide moiety into an acyl-AMP intermediate,followed by nucleophilic substitution with eitherβ-alanine orγ-aminobutyric acid to produce amide derivatives.These findings significantly expand our understanding of PKS-related products originating from C.rosea and also underscore the powerful application of FBMN analytical methods in characterization of new compounds.
基金We gratefully acknowledge the financial support from the National Key Research and Development Program of China(2020YFA0907800)Shandong Jincheng Bio-Pharmaceutical Co.,Ltd.
文摘The global market demand for natural astaxanthin is rapidly increasing owing to its safety,the potential health benefits,and the diverse applications in food and pharmaceutical industries.The major native producers of natural astaxanthin on industrial scale are the alga Haematococcus pluvialis and the yeast Xanthopyllomyces dendrorhous.However,the natural production via these native producers is facing challenges of limited yield and high cost of cultivation and extraction.Alternatively,astaxanthin production via metabolically engineered non-native microbial cell factories such as Escherichia coli,Saccharomyces cerevisiae and Yarrowia lipolytica is another promising strategy to overcome these limitations.In this review we summarize the recent scientific and biotechnological progresses on astaxanthin biosynthetic pathways,transcriptional regulations,the interrelation with lipid metabolism,engineering strategies as well as fermentation process control in major native and non-native astaxanthin producers.These progresses illuminate the prospects of producing astaxanthin by microbial cell factories on industrial scale.
文摘Plant natural products have been widely used in medicine,food,and cosmetics.Of a particular note,many natural products from Cathar-anthus roseus have important biological activities and medicinal value^([1]),with vinblastine as one of the most effective drugs for cancer treatment^([2]).Due to difficulties in chemical synthesis of such complex structures,plant extraction is still the major method for large-scale preparation of plant natural products.
基金We gratefully acknowledge the financial support from the National Key Research and Development Program of China(2020YFA0907800)the National Natural Science Foundation of China(grants 31720103901)This work was also supported by the Open Project Funding of the State Key Laboratory of Bioreactor Engineering,and the 111 Project(B18022).
文摘Excess amount of CO_(2) in the atmosphere is one of the major causes of the greenhouse effect and global warming[1].CO_(2),a promising feedstock for bio-refinery,its efficient utilization is challenging due to its stable structure and low energy state[2,3].However,small energetic compounds like methanol become a bridge and bond of efficient CO_(2) transformation[4,5].Therefore,methanol biotransformation via microbial cell factories will pave the way for sustainable production of value-added chemicals.
