Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination,potentially leading to significantly impaired growth and production in rice.Gaining a comprehensive understandi...Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination,potentially leading to significantly impaired growth and production in rice.Gaining a comprehensive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount importance.In the present study,we identified two R3-MYB genes in rice,namely OsTCL1 and OsTCL2,and characterized their roles in regulating seed germination under salt and drought stress.Plants with tcl1 and tcl2 mutant alleles exhibited delayed seed germination,particularly under stress conditions.The tcl1 tcl2 double mutant showed an even more pronounced reduction in germination during initial stages of germination,thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses.Furthermore,we demonstrated that the transcript levels of several phospholipase D(PLD)genes were downregulated in the tcl1 tcl2 mutant,resulting in a decreased level of the phosphatidic acid(PA)product.Application of 1-butanol,a competitive substrate inhibitor of PLD-dependent production of PA,attenuated the stress response of the tcl1 tcl2 mutant.This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway.Moreover,there were alterations in the expression of genes involved in abscisic acid(ABA)biosynthesis,metabolism and signaling transduction in the double mutant.These changes affected the endogenous ABA level and ABA response,thereby influencing seed germination.Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate(Na2WO4)nearly eliminated the differences in stress response between wild type and the tcl1 tcl2 mutant.This indicates that OsTCL1 and OsTCL2 synergistically coordinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.展开更多
On entering sensory ganglia,herpes simplex viruses 1(HSV-1)establishes a latent infection with the synthesis of a latency associated transcript(LAT)or initiates productive infection with expression of a set of immedia...On entering sensory ganglia,herpes simplex viruses 1(HSV-1)establishes a latent infection with the synthesis of a latency associated transcript(LAT)or initiates productive infection with expression of a set of immediate early viral proteins.The precise mechanisms how expression of a genes is suppressed during the latency are unknown.One mechanism that has been proposed is illustrated in the case of ICP0,a key immediate early viral regulatory protein.Specifically,the 2 kb LAT intron is complementary to the 30 terminal portion of ICP0 m RNA.To test the hypothesis that accumulation of LAT negatively affects the accumulation of ICP0 m RNA,we inserted a DNA fragment encoding two poly(A)sequences into LAT to early terminate LAT transcript without interrupting the complementary sequence of ICP0 transcript(named as SR1603).Comparisons of the parent(SR1601)and mutant(SR1603)HSV-1 viruses showed the following:Neurons harboring latent SR1603 virus accumulated equivalent amounts of viral DNA but higher amounts of ICP0 m RNA and lower amounts of LAT,when compared to neurons harboring the SR1601 virus.One notable difference between the two viruses is that viral RNA accumulation in explanted ganglia harboring SR1603 virus initiated significantly sooner than that in neurons harboring SR1601 virus,suggesting that ICP0 may act as an activator of viral gene expression in permissive cells.Collectively,these data suggest that increased ICP0 m RNA by suppressed LAT did not affect the establishment of latency in latently infected murine ganglia.展开更多
The surrounding geological conditions and supporting structures of underground engineering are often updated during construction,and these updates require repeated numerical modeling.To improve the numerical modeling ...The surrounding geological conditions and supporting structures of underground engineering are often updated during construction,and these updates require repeated numerical modeling.To improve the numerical modeling efficiency of underground engineering,a modularized and parametric modeling cloud server is developed by using Python codes.The basic framework of the cloud server is as follows:input the modeling parameters into the web platform,implement Rhino software and FLAC3D software to model and run simulations in the cloud server,and return the simulation results to the web platform.The modeling program can automatically generate instructions that can run the modeling process in Rhino based on the input modeling parameters.The main modules of the modeling program include modeling the 3D geological structures,the underground engineering structures,and the supporting structures as well as meshing the geometric models.In particular,various cross-sections of underground caverns are crafted as parametricmodules in themodeling program.Themodularized and parametric modeling program is used for a finite element simulation of the underground powerhouse of the Shuangjiangkou Hydropower Station.This complicatedmodel is rapidly generated for the simulation,and the simulation results are reasonable.Thus,this modularized and parametric modeling program is applicable for three-dimensional finite element simulations and analyses.展开更多
For conjugated organic radical systems,the comprehension and regulation of spin interactions form the cornerstone of their application in electronic devices and magnetic materials.In this report,employing a dual strat...For conjugated organic radical systems,the comprehension and regulation of spin interactions form the cornerstone of their application in electronic devices and magnetic materials.In this report,employing a dual strategy of modulating substituents and reducing agents,we synthesize three naphthalenediimide(NDI)anion radical crystals with distinct stacking structures.They are classified as monomer,π-Dimer,andπ-Stack based on the form of NDI existence.For the latter two,comparative analysis with the corresponding neutral NDI crystals reveals that the NDI radicals exhibit closerπ–πdistances and largerπorbital overlap areas,unequivocally confirming the presence of spin(singly occupied molecular orbital)interactions.Furthermore,variations in the crystal environments of the spin centers lead to differences in spin–spin interactions,as demonstrated by solidstate absorption spectra and electron paramagnetic resonance(EPR)spectroscopy.The monomer crystal displays a single-molecule absorption spectrum akin to that in solution and undiminished EPR intensity,suggesting negligible spin exchange interaction;π-dimer andπ-stack exhibit significant blueshifts in visible light absorption and characteristic near-infrared absorption,attributed to the newly formed electronic structure of the aggregates.Additionally,their EPR integral intensity and temperature-dependent behavior indicate an antiferromagnetic ground state,corresponding to strong spin–spin pairing effects.展开更多
Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing the retention of the native extracellular matrix structure,mechanical in...Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing the retention of the native extracellular matrix structure,mechanical integrity and bioactivity.The decellularized tissue obtained through the tissue decellularization technique retains the structure and bioactive components of its native tissue;it not only exhibits comparatively strong mechanical prop-erties,low immunogenicity and good biocompatibility but also stimulates in situ neovascularization at the implantation site and regulates the polarization process of recruited macrophages,thereby promoting the regeneration of damaged tissue.Consequently,many commercial products have been developed as promising therapeutic strategies for the treatment of different tissue defects and lesions,such as wounds,dura,bone and cartilage defects,nerve injuries,myocardial infarction,urethral strictures,corneal blindness and other orthopedic applications.Recently,there has been a growing interest in the decellularization of fish tissues because of the abundance of sources,less religious constraints and risks of zoonosis transmission between mammals.In this review,we provide a complete overview of the state-of-the-art decellularization of fish tissues,including the organs and methods used to prepare acellular tissues.We enumerated common decellularized fish tissues from various fish organs,such as skin,scale,bladder,cartilage,heart and brain,and elaborated their different processing methods and tissue engineering applications.Furthermore,we presented the perspectives of(i)the future development direction of fish tissue decellulariza-tion technology,(ii)expanding the sources of decellularized tissue and(iii)innovating decellularized tissue bio-inks for 3D bioprinting to unleash the great potential of decellularized tissue in tissue engineering and regenerative medicine applications.展开更多
Hydrogen sulfide(H_(2)S)-based mitochondrial energy metabolism blockade is an attractive tumor therapeutic modality.However,it is limited owing to metabolic plasticity,which allows tumors to shift their metabolic phen...Hydrogen sulfide(H_(2)S)-based mitochondrial energy metabolism blockade is an attractive tumor therapeutic modality.However,it is limited owing to metabolic plasticity,which allows tumors to shift their metabolic phenotype between oxidative phosphorylation and glycolysis for energy compensation.Herein,a hollow-hierarchical H_(2)S-multistage blasting nanomedicine was designed for a dual-pathway strategy targeting the blockade of energy metabolism and the imbalance of redox homeostasis.The tetrasulfide bond-modified hollow-hierarchical structure presents in-situ H_(2)S long-term bursting under the intracellular overexpressed glutathione(GSH),which inhibits the expression of the electron transport chain complex cytochrome C(COX IV)for restraining mitochondrial bioenergy supply and causes the energy metabolism blockade.Meanwhile,the Prussian blue in the home position,with thermal-enhanced peroxidase enzymatic activity,could simultaneously generate highly toxic hydroxyl radicals and exacerbate the GSH depletion process,thus further disrupting intracellular redox homeostasis.Mainly,externally encapsulated calcium can induce intracellular acidification and calcium overload,which aggravates mitochondrial dysfunction.The loaded glucose oxidase competes for intracellular glycolytic substrates,generating endogenous H_(2)O_(2) while inhibiting COX IV activity and rapidly depleting intracellular adenosine in triphosphate,thus completely blocking the energy supply of tumor cells.This dual-pathway strategy utilizes H_(2)S gas-bloomed calcium overload to block energy metabolism and induce redox imbalance,providing new insights into exploring energy metabolism blockade as a therapeutic tool for tumor treatment.展开更多
Purpose–This paper aims to review the studies on intersection control with connected and automated vehicles(CAVs).Design/methodology/approach–The most seminal and recent research in this area is reviewed.This study ...Purpose–This paper aims to review the studies on intersection control with connected and automated vehicles(CAVs).Design/methodology/approach–The most seminal and recent research in this area is reviewed.This study specifically focuses on two categories:CAV trajectory planning and joint intersection and CAV control.Findings–It is found that there is a lack of widely recognized benchmarks in this area,which hinders the validation and demonstration of new studies.Originality/value–In this review,the authors focus on the methodological approaches taken to empower intersection control with CAVs.The authors hope the present review could shed light on the state-of-the-art methods,research gaps and future research directions.展开更多
1.Introduction With the advance of data collection,data processing,telecommunication and vehicular technologies,connected vehicles(CVs)have been emerging as a crucial branch of smart mobility(Olia et al.,2015;Li et al...1.Introduction With the advance of data collection,data processing,telecommunication and vehicular technologies,connected vehicles(CVs)have been emerging as a crucial branch of smart mobility(Olia et al.,2015;Li et al.,2021).Its basic idea is to realize real-time exchanges and processing of essential information,such as positions and destinations,among surrounding vehicles and infrastructures.展开更多
Purpose–This paper aims to address the longitudinal control problem for person-following robots(PFRs)for the implementation of this technology.Design/methodology/approach–Nine representative car-following models are...Purpose–This paper aims to address the longitudinal control problem for person-following robots(PFRs)for the implementation of this technology.Design/methodology/approach–Nine representative car-following models are analyzed from PFRs application and the linear model and optimal velocity model/full velocity difference model are qualified and selected in the PFR control.Findings–A lab PFR with the bar-laser-perception device is developed and tested in the field,and the results indicate that the proposed models perform well in normal person-following scenarios.Originality/value–This study fills a gap in the research on PRFs longitudinal control and provides a useful and practical reference on PFRs longitudinal control for the related research.展开更多
Purpose–This study aims to make full use of the advantages of connected and autonomous vehicles(CAVs)and dedicated CAV lanes to ensure all CAVs can pass intersections without stopping.Design/methodology/approach–The...Purpose–This study aims to make full use of the advantages of connected and autonomous vehicles(CAVs)and dedicated CAV lanes to ensure all CAVs can pass intersections without stopping.Design/methodology/approach–The authors developed a signal coordination model for arteries with dedicated CAV lanes by using mixed integer linear programming.CAV non-stop constraints are proposed to adapt to the characteristics of CAVs.As it is a continuous problem,various situations that CAVs arrive at intersections are analyzed.The rules are discovered to simplify the problem by discretization method.Findings–A case study is conducted via SUMO traffic simulation program.The results show that the efficiency of CAVs can be improved significantly both in high-volume scenario and medium-volume scenario with the plan optimized by the model proposed in this paper.At the same time,the progression efficiency of regular vehicles is not affected significantly.It is indicated that full-scale benefits of dedicated CAV lanes can only be achieved with signal coordination plans considering CAV characteristics.Originality/value–To the best of the authors’knowledge,this is the first research that develops a signal coordination model for arteries with dedicated CAV lanes.展开更多
Shared electric scooters(e-scooter)are booming across the world and widely regarded as a sustainable mobility service.An increasing number of studies have investigated the e-scooter trip patterns,safety risks,and envi...Shared electric scooters(e-scooter)are booming across the world and widely regarded as a sustainable mobility service.An increasing number of studies have investigated the e-scooter trip patterns,safety risks,and environmental impacts,but few considered the energy efficiency of e-scooters.In this research,we collected the operational data of e-scooters from a major provider in Gothenburg to shed light on the energy efficiency performance of e-scooters in real cases.We first develop a multiple logarithmic regression model to examine the energy consumption of single trips and influencing factors.With the regression model,a Monte Carlo simulation framework is proposed to estimate the fleet energy consumption in various scenarios,taking into account both trip-related energy usage and energy loss in idle status.The results indicate that 40%of e-scooter battery energy was wasted in idle status in the current practice,mainly due to the relatively low usage rate(0.83)of e-scooters.If the average usage rate drops below 0.5,the wasted energy could reach up to 53%.In the end,we present a field example to showcase how to optimally integrate public transport with e-scooters from the perspective of energy efficiency.We hope the findings of this study could help understand and resolve the current and future challenges regarding the ever-growing e-scooter services.展开更多
In this paper,we develop a profit-sharing-based optimal routing mechanism to incentivize horizontal collaboration among urban goods distributors.The core of this mechanism is based on exchanging goods at meet points,w...In this paper,we develop a profit-sharing-based optimal routing mechanism to incentivize horizontal collaboration among urban goods distributors.The core of this mechanism is based on exchanging goods at meet points,which is optimally planned en route.We propose a Collaborative Electric Vehicle Routing Problem with Meet Points(CoEVRPMP)considering constraints such as time windows,opportunity charging,and meet-point synchronization.The proposed CoEVRPMP is formulated as a mixed-integer nonlinear programming model.We present an exact method via branching and a matheuristic that combines adaptive large neighborhood search with linear programming.The viability and scalability of the collaborative method are demonstrated through numerical case studies,including a real-world case and a large-scale experiment with up to 500 customers.The findings underscore the significance of horizontal collaboration among delivery companies in attaining both higher individual profits and lower total costs.Moreover,collaboration helps to reduce the environmental footprint by decreasing travel distance.展开更多
Academic papers are the cornerstone of knowledge dissemination and crucial for researchers’career development.This is particularly true for rapidly evolving research domains such as transportation,as evidenced by the...Academic papers are the cornerstone of knowledge dissemination and crucial for researchers’career development.This is particularly true for rapidly evolving research domains such as transportation,as evidenced by the surge of journals and papers in the past decade.While abundant literature offers guidance on successful publication strategies,insights into the reasons for rejection are rare.This study fills in this gap by examining why papers are rejected in the area of transportation.We present concrete evidence based on data from over 5,000 rejected transport papers.Quantitative analyses are conducted to reveal the impacts of similarity rate,duplication submission rate,and topic on desk rejections.Additionally,we shed light on the distinct focus reviewers have when serving different journals.We hope the results could equip transport researchers with a deeper comprehension of publication criteria and a better awareness of common but avoidable mistakes.展开更多
基金the National Natural Science Foundation of China(31970603)Natural Science Foundation of Guangxi province(2019GXNSFDA185001)+1 种基金State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources(SKLCUSA-a202008 and SKLCUSA-a01)Guangxi University-Bama Research Fund(20220006).
文摘Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination,potentially leading to significantly impaired growth and production in rice.Gaining a comprehensive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount importance.In the present study,we identified two R3-MYB genes in rice,namely OsTCL1 and OsTCL2,and characterized their roles in regulating seed germination under salt and drought stress.Plants with tcl1 and tcl2 mutant alleles exhibited delayed seed germination,particularly under stress conditions.The tcl1 tcl2 double mutant showed an even more pronounced reduction in germination during initial stages of germination,thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses.Furthermore,we demonstrated that the transcript levels of several phospholipase D(PLD)genes were downregulated in the tcl1 tcl2 mutant,resulting in a decreased level of the phosphatidic acid(PA)product.Application of 1-butanol,a competitive substrate inhibitor of PLD-dependent production of PA,attenuated the stress response of the tcl1 tcl2 mutant.This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway.Moreover,there were alterations in the expression of genes involved in abscisic acid(ABA)biosynthesis,metabolism and signaling transduction in the double mutant.These changes affected the endogenous ABA level and ABA response,thereby influencing seed germination.Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate(Na2WO4)nearly eliminated the differences in stress response between wild type and the tcl1 tcl2 mutant.This indicates that OsTCL1 and OsTCL2 synergistically coordinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.
基金supported by grants from Shenzhen Overseas High-Caliber Peacock Foundation KQTD2015071414385495Shenzhen Science and Innovation Commission Project Grants JCYJ20180306173333907 to Shenzhen International Institute for Biomedical Research。
文摘On entering sensory ganglia,herpes simplex viruses 1(HSV-1)establishes a latent infection with the synthesis of a latency associated transcript(LAT)or initiates productive infection with expression of a set of immediate early viral proteins.The precise mechanisms how expression of a genes is suppressed during the latency are unknown.One mechanism that has been proposed is illustrated in the case of ICP0,a key immediate early viral regulatory protein.Specifically,the 2 kb LAT intron is complementary to the 30 terminal portion of ICP0 m RNA.To test the hypothesis that accumulation of LAT negatively affects the accumulation of ICP0 m RNA,we inserted a DNA fragment encoding two poly(A)sequences into LAT to early terminate LAT transcript without interrupting the complementary sequence of ICP0 transcript(named as SR1603).Comparisons of the parent(SR1601)and mutant(SR1603)HSV-1 viruses showed the following:Neurons harboring latent SR1603 virus accumulated equivalent amounts of viral DNA but higher amounts of ICP0 m RNA and lower amounts of LAT,when compared to neurons harboring the SR1601 virus.One notable difference between the two viruses is that viral RNA accumulation in explanted ganglia harboring SR1603 virus initiated significantly sooner than that in neurons harboring SR1601 virus,suggesting that ICP0 may act as an activator of viral gene expression in permissive cells.Collectively,these data suggest that increased ICP0 m RNA by suppressed LAT did not affect the establishment of latency in latently infected murine ganglia.
基金The Construction S&T Project of the Department of Transportation of Sichuan Province(Grant No.2023A02)the National Natural Science Foundation of China(No.52109135).
文摘The surrounding geological conditions and supporting structures of underground engineering are often updated during construction,and these updates require repeated numerical modeling.To improve the numerical modeling efficiency of underground engineering,a modularized and parametric modeling cloud server is developed by using Python codes.The basic framework of the cloud server is as follows:input the modeling parameters into the web platform,implement Rhino software and FLAC3D software to model and run simulations in the cloud server,and return the simulation results to the web platform.The modeling program can automatically generate instructions that can run the modeling process in Rhino based on the input modeling parameters.The main modules of the modeling program include modeling the 3D geological structures,the underground engineering structures,and the supporting structures as well as meshing the geometric models.In particular,various cross-sections of underground caverns are crafted as parametricmodules in themodeling program.Themodularized and parametric modeling program is used for a finite element simulation of the underground powerhouse of the Shuangjiangkou Hydropower Station.This complicatedmodel is rapidly generated for the simulation,and the simulation results are reasonable.Thus,this modularized and parametric modeling program is applicable for three-dimensional finite element simulations and analyses.
基金financially supported by the National Key R&D Program of China(grant no.2020YFA0714604)the Natural Science Foundation of China(grant nos.U20A6002 and 52203221)+1 种基金the Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates(grant no.2023B1212060003)the open research fund of Songshan Lake Materials Laboratory(grant no.2023SLABFK05).
文摘For conjugated organic radical systems,the comprehension and regulation of spin interactions form the cornerstone of their application in electronic devices and magnetic materials.In this report,employing a dual strategy of modulating substituents and reducing agents,we synthesize three naphthalenediimide(NDI)anion radical crystals with distinct stacking structures.They are classified as monomer,π-Dimer,andπ-Stack based on the form of NDI existence.For the latter two,comparative analysis with the corresponding neutral NDI crystals reveals that the NDI radicals exhibit closerπ–πdistances and largerπorbital overlap areas,unequivocally confirming the presence of spin(singly occupied molecular orbital)interactions.Furthermore,variations in the crystal environments of the spin centers lead to differences in spin–spin interactions,as demonstrated by solidstate absorption spectra and electron paramagnetic resonance(EPR)spectroscopy.The monomer crystal displays a single-molecule absorption spectrum akin to that in solution and undiminished EPR intensity,suggesting negligible spin exchange interaction;π-dimer andπ-stack exhibit significant blueshifts in visible light absorption and characteristic near-infrared absorption,attributed to the newly formed electronic structure of the aggregates.Additionally,their EPR integral intensity and temperature-dependent behavior indicate an antiferromagnetic ground state,corresponding to strong spin–spin pairing effects.
基金supported by the Taizhou Science and Technology Plan Project(22ywb146)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C04043)+1 种基金National Natural Science Foundation of China(22377021)the startup grants from Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2019002,WIUCASQD2021025).
文摘Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing the retention of the native extracellular matrix structure,mechanical integrity and bioactivity.The decellularized tissue obtained through the tissue decellularization technique retains the structure and bioactive components of its native tissue;it not only exhibits comparatively strong mechanical prop-erties,low immunogenicity and good biocompatibility but also stimulates in situ neovascularization at the implantation site and regulates the polarization process of recruited macrophages,thereby promoting the regeneration of damaged tissue.Consequently,many commercial products have been developed as promising therapeutic strategies for the treatment of different tissue defects and lesions,such as wounds,dura,bone and cartilage defects,nerve injuries,myocardial infarction,urethral strictures,corneal blindness and other orthopedic applications.Recently,there has been a growing interest in the decellularization of fish tissues because of the abundance of sources,less religious constraints and risks of zoonosis transmission between mammals.In this review,we provide a complete overview of the state-of-the-art decellularization of fish tissues,including the organs and methods used to prepare acellular tissues.We enumerated common decellularized fish tissues from various fish organs,such as skin,scale,bladder,cartilage,heart and brain,and elaborated their different processing methods and tissue engineering applications.Furthermore,we presented the perspectives of(i)the future development direction of fish tissue decellulariza-tion technology,(ii)expanding the sources of decellularized tissue and(iii)innovating decellularized tissue bio-inks for 3D bioprinting to unleash the great potential of decellularized tissue in tissue engineering and regenerative medicine applications.
基金supported by the National Natural Science Foundation of China(Nos.22205048 and U22A20347)the China Postdoctoral Science Foundation(No.2023T160154)+2 种基金the Heilongjiang Postdoctoral Science Foundation(No.LBH-TZ2406)the Young Elite Scientists Sponsorship Program of Heilongjiang(No.2024QNTJ001)the Fundamental Research Funds for the Central Universities.
文摘Hydrogen sulfide(H_(2)S)-based mitochondrial energy metabolism blockade is an attractive tumor therapeutic modality.However,it is limited owing to metabolic plasticity,which allows tumors to shift their metabolic phenotype between oxidative phosphorylation and glycolysis for energy compensation.Herein,a hollow-hierarchical H_(2)S-multistage blasting nanomedicine was designed for a dual-pathway strategy targeting the blockade of energy metabolism and the imbalance of redox homeostasis.The tetrasulfide bond-modified hollow-hierarchical structure presents in-situ H_(2)S long-term bursting under the intracellular overexpressed glutathione(GSH),which inhibits the expression of the electron transport chain complex cytochrome C(COX IV)for restraining mitochondrial bioenergy supply and causes the energy metabolism blockade.Meanwhile,the Prussian blue in the home position,with thermal-enhanced peroxidase enzymatic activity,could simultaneously generate highly toxic hydroxyl radicals and exacerbate the GSH depletion process,thus further disrupting intracellular redox homeostasis.Mainly,externally encapsulated calcium can induce intracellular acidification and calcium overload,which aggravates mitochondrial dysfunction.The loaded glucose oxidase competes for intracellular glycolytic substrates,generating endogenous H_(2)O_(2) while inhibiting COX IV activity and rapidly depleting intracellular adenosine in triphosphate,thus completely blocking the energy supply of tumor cells.This dual-pathway strategy utilizes H_(2)S gas-bloomed calcium overload to block energy metabolism and induce redox imbalance,providing new insights into exploring energy metabolism blockade as a therapeutic tool for tumor treatment.
基金supported by the National Natural Science Foundation of China(22278056)Liaoning Revitalization Talent Program(XLYC2008032)the Fundamental Research Funds for the Central Universities(DUT22LAB602)。
文摘Purpose–This paper aims to review the studies on intersection control with connected and automated vehicles(CAVs).Design/methodology/approach–The most seminal and recent research in this area is reviewed.This study specifically focuses on two categories:CAV trajectory planning and joint intersection and CAV control.Findings–It is found that there is a lack of widely recognized benchmarks in this area,which hinders the validation and demonstration of new studies.Originality/value–In this review,the authors focus on the methodological approaches taken to empower intersection control with CAVs.The authors hope the present review could shed light on the state-of-the-art methods,research gaps and future research directions.
文摘1.Introduction With the advance of data collection,data processing,telecommunication and vehicular technologies,connected vehicles(CVs)have been emerging as a crucial branch of smart mobility(Olia et al.,2015;Li et al.,2021).Its basic idea is to realize real-time exchanges and processing of essential information,such as positions and destinations,among surrounding vehicles and infrastructures.
基金supported by the Basal Research Fund of Central Public Research Institute of China(Grant No.20212702).
文摘Purpose–This paper aims to address the longitudinal control problem for person-following robots(PFRs)for the implementation of this technology.Design/methodology/approach–Nine representative car-following models are analyzed from PFRs application and the linear model and optimal velocity model/full velocity difference model are qualified and selected in the PFR control.Findings–A lab PFR with the bar-laser-perception device is developed and tested in the field,and the results indicate that the proposed models perform well in normal person-following scenarios.Originality/value–This study fills a gap in the research on PRFs longitudinal control and provides a useful and practical reference on PFRs longitudinal control for the related research.
基金supported by“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2022C01042),and Alibaba-Zhejiang University Joint Research Institute of Frontier Technologies.
文摘Purpose–This study aims to make full use of the advantages of connected and autonomous vehicles(CAVs)and dedicated CAV lanes to ensure all CAVs can pass intersections without stopping.Design/methodology/approach–The authors developed a signal coordination model for arteries with dedicated CAV lanes by using mixed integer linear programming.CAV non-stop constraints are proposed to adapt to the characteristics of CAVs.As it is a continuous problem,various situations that CAVs arrive at intersections are analyzed.The rules are discovered to simplify the problem by discretization method.Findings–A case study is conducted via SUMO traffic simulation program.The results show that the efficiency of CAVs can be improved significantly both in high-volume scenario and medium-volume scenario with the plan optimized by the model proposed in this paper.At the same time,the progression efficiency of regular vehicles is not affected significantly.It is indicated that full-scale benefits of dedicated CAV lanes can only be achieved with signal coordination plans considering CAV characteristics.Originality/value–To the best of the authors’knowledge,this is the first research that develops a signal coordination model for arteries with dedicated CAV lanes.
文摘Shared electric scooters(e-scooter)are booming across the world and widely regarded as a sustainable mobility service.An increasing number of studies have investigated the e-scooter trip patterns,safety risks,and environmental impacts,but few considered the energy efficiency of e-scooters.In this research,we collected the operational data of e-scooters from a major provider in Gothenburg to shed light on the energy efficiency performance of e-scooters in real cases.We first develop a multiple logarithmic regression model to examine the energy consumption of single trips and influencing factors.With the regression model,a Monte Carlo simulation framework is proposed to estimate the fleet energy consumption in various scenarios,taking into account both trip-related energy usage and energy loss in idle status.The results indicate that 40%of e-scooter battery energy was wasted in idle status in the current practice,mainly due to the relatively low usage rate(0.83)of e-scooters.If the average usage rate drops below 0.5,the wasted energy could reach up to 53%.In the end,we present a field example to showcase how to optimally integrate public transport with e-scooters from the perspective of energy efficiency.We hope the findings of this study could help understand and resolve the current and future challenges regarding the ever-growing e-scooter services.
基金Transport Area of Advanced within Chalmers University of Technology through the project COLLECT:Horizontal Cooperation in Urban Distribution Logistics-a Trusted-Cooperative Electric Vehicle Routing Method.This work was in part supported by the European Commission via the ERA-Net Urban Accessibility and Connectivity Sino-European program and the project E-LaaS:Energy optimal urban Logistics As A Service and by the Swedish Energy Agency(P2023-00021).
文摘In this paper,we develop a profit-sharing-based optimal routing mechanism to incentivize horizontal collaboration among urban goods distributors.The core of this mechanism is based on exchanging goods at meet points,which is optimally planned en route.We propose a Collaborative Electric Vehicle Routing Problem with Meet Points(CoEVRPMP)considering constraints such as time windows,opportunity charging,and meet-point synchronization.The proposed CoEVRPMP is formulated as a mixed-integer nonlinear programming model.We present an exact method via branching and a matheuristic that combines adaptive large neighborhood search with linear programming.The viability and scalability of the collaborative method are demonstrated through numerical case studies,including a real-world case and a large-scale experiment with up to 500 customers.The findings underscore the significance of horizontal collaboration among delivery companies in attaining both higher individual profits and lower total costs.Moreover,collaboration helps to reduce the environmental footprint by decreasing travel distance.
文摘Academic papers are the cornerstone of knowledge dissemination and crucial for researchers’career development.This is particularly true for rapidly evolving research domains such as transportation,as evidenced by the surge of journals and papers in the past decade.While abundant literature offers guidance on successful publication strategies,insights into the reasons for rejection are rare.This study fills in this gap by examining why papers are rejected in the area of transportation.We present concrete evidence based on data from over 5,000 rejected transport papers.Quantitative analyses are conducted to reveal the impacts of similarity rate,duplication submission rate,and topic on desk rejections.Additionally,we shed light on the distinct focus reviewers have when serving different journals.We hope the results could equip transport researchers with a deeper comprehension of publication criteria and a better awareness of common but avoidable mistakes.
