Benign prostatic hyperplasia(BPH)is one of the major chronic complications of type 2 diabetes mellitus(T2DM),and sex steroid hormones are common risk factors for the occurrence of T2DM and BPH.The profiles of sex ster...Benign prostatic hyperplasia(BPH)is one of the major chronic complications of type 2 diabetes mellitus(T2DM),and sex steroid hormones are common risk factors for the occurrence of T2DM and BPH.The profiles of sex steroid hormones are simultaneously quantified by LC-MS/MS in the clinical serum of patients,including simple BPH patients,newly diagnosed T2DM patients,T2DM complicated with BPH patients and matched healthy individuals.The G protein-coupled estrogen receptor(GPER)inhibitor G15,GPER knockdown lentivirus,the YAP1 inhibitor verteporfin,YAP1 knockdown/overexpression lentivirus,targeted metabolomics analysis,and Co-IP assays are used to investigate the molecular mechanisms of the disrupted sex steroid hormones homeostasis in the pathological process of T2DM complicated with BPH.The homeostasis of sex steroid hormone is disrupted in the serum of patients,accompanying with the proliferated prostatic epithelial cells(PECs).The sex steroid hormone metabolic profiles of T2DM patients complicated with BPH have the greatest degrees of separation from those of healthy individuals.Elevated 17β-estradiol(E2)is the key contributor to the disrupted sex steroid hormone homeostasis,and is significantly positively related to the clinical characteristics of T2DM patients complicated with BPH.Activating GPER by E2 via Hippo-YAP1 signaling exacerbates high glucose(HG)-induced PECs proliferation through the formation of the YAP1-TEAD4 heterodimer.Knockdown or inhibition of GPER-mediated Hippo-YAP1 signaling suppresses PECs proliferation in HG and E2 co-treated BPH-1 cells.The anti-proliferative effects of verteporfin,an inhibitor of YAP1,are blocked by YAP1 overexpression in HG and E2 co-treated BPH-1 cells.Inactivating E2/GPER/Hippo/YAP1 signaling may be effective at delaying the progression of T2DM complicated with BPH by inhibiting PECs proliferation.展开更多
The abuse of plastic food packaging has brought about severe white pollution issues around the world.Developing green and sustainable biomass packaging is an effective way to solve this problem.Hence,a chitosan/sodium...The abuse of plastic food packaging has brought about severe white pollution issues around the world.Developing green and sustainable biomass packaging is an effective way to solve this problem.Hence,a chitosan/sodium alginate-based multilayer film is fabricated via a layer-by-layer(LBL)self-assembly method.With the help of superior interaction between the layers,the multilayer film possesses excellent mechanical properties(with a tensile strength of 50 MPa).Besides,the film displays outstanding water retention property(blocking moisture of 97.56%)and ultraviolet blocking property.Anthocyanin is introduced into the film to detect the food quality since it is one natural plant polyphenol that is sensitive to the pH changes ranging from 1 to 13 in food when spoilage occurs.It is noted that the film is also bacteriostatic which is desired for food packaging.This study describes a simple technique for the development of advanced multifunctional and fully biodegradable food packaging film and it is a sustainable alternative to plastic packaging.展开更多
Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of tw...Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of two different homozygous inbred lines has long been applied to increase crop yield.Doubled haploid(DH)technology greatly shortens the breeding process of homozygous lines by up to 3-5 years.In recent years,new haploid induction(HI)techniques based on genes such as DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(DMP)and CENTROMERIC HISTONE3(CENH3)were exciting progresses in generating DHs in a more efficient and simple way(Jiang et al.,2022;Qu et al.,2024).展开更多
The performance of complementary metal oxide semiconductor(CMOS)circuits is affected by electromagnetic interference(EMI),and the study of the circuit's ability to resist EMI will facilitate the design of circuits...The performance of complementary metal oxide semiconductor(CMOS)circuits is affected by electromagnetic interference(EMI),and the study of the circuit's ability to resist EMI will facilitate the design of circuits with better performance.Current-mode CMOS circuits have been continuously developed in recent years due to their advantages of high speed and low power consumption over conventional circuits under the deep submicron process;their EMI resistance performance deserves further study.This paper introduces three kinds of NOT gate circuits:conventional voltage-mode CMOS,MOS current-mode logic(MCML)with voltage signal of input and output,and current-mode CMOS with current signal of input and output.The effects of EMI on three NOT gate circuits are investigated using Cadence Virtuoso software simulation,and a disturbance level factor is defined to compare the effects of different interference terminals,interference signals'waveforms,and interference signals'frequencies on the circuits in the 65 nm process.The relationship between input resistance and circuit EMI resistance performance is investigated by varying the value of cascade resistance at the input of the current-mode CMOS circuits.Simulation results show that the current-mode CMOS circuits have better resistance performance to EMI at high operating frequencies,and the higher the operating frequency of the current-mode CMOS circuits,the better the resistance performance of the circuits to EMI.Additionally,the effects of different temperatures and different processes on the resistance performance of three circuits are also studied.In the temperature range of-40℃to 125℃,the higher the temperature,the weaker the resistance ability of voltage-mode CMOS and MCML circuits,and the stronger the resistance ability of current-mode CMOS circuits.In the 28 nm process,the current-mode CMOS circuit interference resistance ability is relatively stronger than that of the other two kinds of circuits.The relative interference resistance ability of voltage-mode CMOS and MCML circuits in the 28 nm process is similar to that of the 65 nm process,while the relative interference resistance ability of current-mode CMOS circuits in the 28 nm process is stronger than that of the 65 nm process.This study provides a basis for the design of current-mode CMOS circuits against EMI.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:82073906 and 82273987)Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions,and Postgraduate Research Practice Innovation Program of Jiangsu Province(Grant Nos.:KYCX22-2966 and KYCX23-2967).
文摘Benign prostatic hyperplasia(BPH)is one of the major chronic complications of type 2 diabetes mellitus(T2DM),and sex steroid hormones are common risk factors for the occurrence of T2DM and BPH.The profiles of sex steroid hormones are simultaneously quantified by LC-MS/MS in the clinical serum of patients,including simple BPH patients,newly diagnosed T2DM patients,T2DM complicated with BPH patients and matched healthy individuals.The G protein-coupled estrogen receptor(GPER)inhibitor G15,GPER knockdown lentivirus,the YAP1 inhibitor verteporfin,YAP1 knockdown/overexpression lentivirus,targeted metabolomics analysis,and Co-IP assays are used to investigate the molecular mechanisms of the disrupted sex steroid hormones homeostasis in the pathological process of T2DM complicated with BPH.The homeostasis of sex steroid hormone is disrupted in the serum of patients,accompanying with the proliferated prostatic epithelial cells(PECs).The sex steroid hormone metabolic profiles of T2DM patients complicated with BPH have the greatest degrees of separation from those of healthy individuals.Elevated 17β-estradiol(E2)is the key contributor to the disrupted sex steroid hormone homeostasis,and is significantly positively related to the clinical characteristics of T2DM patients complicated with BPH.Activating GPER by E2 via Hippo-YAP1 signaling exacerbates high glucose(HG)-induced PECs proliferation through the formation of the YAP1-TEAD4 heterodimer.Knockdown or inhibition of GPER-mediated Hippo-YAP1 signaling suppresses PECs proliferation in HG and E2 co-treated BPH-1 cells.The anti-proliferative effects of verteporfin,an inhibitor of YAP1,are blocked by YAP1 overexpression in HG and E2 co-treated BPH-1 cells.Inactivating E2/GPER/Hippo/YAP1 signaling may be effective at delaying the progression of T2DM complicated with BPH by inhibiting PECs proliferation.
基金National Undergraduate Training Program for Innovation and Entrepreneurship of China (Grant No.202210288027).
文摘The abuse of plastic food packaging has brought about severe white pollution issues around the world.Developing green and sustainable biomass packaging is an effective way to solve this problem.Hence,a chitosan/sodium alginate-based multilayer film is fabricated via a layer-by-layer(LBL)self-assembly method.With the help of superior interaction between the layers,the multilayer film possesses excellent mechanical properties(with a tensile strength of 50 MPa).Besides,the film displays outstanding water retention property(blocking moisture of 97.56%)and ultraviolet blocking property.Anthocyanin is introduced into the film to detect the food quality since it is one natural plant polyphenol that is sensitive to the pH changes ranging from 1 to 13 in food when spoilage occurs.It is noted that the film is also bacteriostatic which is desired for food packaging.This study describes a simple technique for the development of advanced multifunctional and fully biodegradable food packaging film and it is a sustainable alternative to plastic packaging.
基金supported by grants from the National Key R&D Program of China(2023YFD1201501)the Key Technology R&D Program of Jiangsu Province(BE2023366)+1 种基金the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)the China Agriculture Research System of MOF and MARA(CARS-23).
文摘Global food production is a major challenge currently facing humanity,and the primary way to secure global food security is to increase crop yields and enhance nutritional value.Heterosis utilization by crossing of two different homozygous inbred lines has long been applied to increase crop yield.Doubled haploid(DH)technology greatly shortens the breeding process of homozygous lines by up to 3-5 years.In recent years,new haploid induction(HI)techniques based on genes such as DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(DMP)and CENTROMERIC HISTONE3(CENH3)were exciting progresses in generating DHs in a more efficient and simple way(Jiang et al.,2022;Qu et al.,2024).
文摘The performance of complementary metal oxide semiconductor(CMOS)circuits is affected by electromagnetic interference(EMI),and the study of the circuit's ability to resist EMI will facilitate the design of circuits with better performance.Current-mode CMOS circuits have been continuously developed in recent years due to their advantages of high speed and low power consumption over conventional circuits under the deep submicron process;their EMI resistance performance deserves further study.This paper introduces three kinds of NOT gate circuits:conventional voltage-mode CMOS,MOS current-mode logic(MCML)with voltage signal of input and output,and current-mode CMOS with current signal of input and output.The effects of EMI on three NOT gate circuits are investigated using Cadence Virtuoso software simulation,and a disturbance level factor is defined to compare the effects of different interference terminals,interference signals'waveforms,and interference signals'frequencies on the circuits in the 65 nm process.The relationship between input resistance and circuit EMI resistance performance is investigated by varying the value of cascade resistance at the input of the current-mode CMOS circuits.Simulation results show that the current-mode CMOS circuits have better resistance performance to EMI at high operating frequencies,and the higher the operating frequency of the current-mode CMOS circuits,the better the resistance performance of the circuits to EMI.Additionally,the effects of different temperatures and different processes on the resistance performance of three circuits are also studied.In the temperature range of-40℃to 125℃,the higher the temperature,the weaker the resistance ability of voltage-mode CMOS and MCML circuits,and the stronger the resistance ability of current-mode CMOS circuits.In the 28 nm process,the current-mode CMOS circuit interference resistance ability is relatively stronger than that of the other two kinds of circuits.The relative interference resistance ability of voltage-mode CMOS and MCML circuits in the 28 nm process is similar to that of the 65 nm process,while the relative interference resistance ability of current-mode CMOS circuits in the 28 nm process is stronger than that of the 65 nm process.This study provides a basis for the design of current-mode CMOS circuits against EMI.
