Quantitative phase imaging(QPI)has emerged as a valuable tool for biomedical research thanks to its unique capabilities for quantifying optical thickness variation of living cells and tissues.Among many QPI methods,Fo...Quantitative phase imaging(QPI)has emerged as a valuable tool for biomedical research thanks to its unique capabilities for quantifying optical thickness variation of living cells and tissues.Among many QPI methods,Fourier ptychographic microscopy(FPM)allows long-term label-free observation and quantitative analysis of large cell populations without compromising spatial and temporal resolution.However,high spatio-temporal resolution imaging over a long-time scale(from hours to days)remains a critical challenge:optically inhomogeneous structure of biological specimens as well as mechanical perturbations and thermal fluctuations of the microscope body all result in time-varying aberration and focus drifts,significantly degrading the imaging performance for long-term study.Moreover,the aberrations are sample-and environmentdependent,and cannot be compensated by a fixed optical design,thus necessitating rapid dynamic correction in the imaging process.Here,we report an adaptive optical QPI method based on annular illumination FPM.In this method,the annular matched illumination configuration(i.e.,the illumination numerical aperture(NA)strictly equals to the objective NA),which is the key for recovering low-frequency phase information,is further utilized for the accurate imaging aberration characterization.By using only 6 low-resolution images captured with 6 different illumination angles matching the NA of a 10x,0.4 NA objective,we recover high-resolution quantitative phase images(synthetic NA of 0.8)and characterize the aberrations in real time,restoring the optimum resolution of the system adaptively.Applying our method to live-cell imaging,we achieve diffraction-limited performance(full-pitch resolution of 655 nm at a wavelength of 525 nm)across a wide field of view(1.77mm2)over an extended period of time.展开更多
Chiral enantiomers have different pharmacological and pharmacokinetic characteristics. It is important to strictly detect chiral component for avoiding being harmful to the human body due to side effects. Terahertz (T...Chiral enantiomers have different pharmacological and pharmacokinetic characteristics. It is important to strictly detect chiral component for avoiding being harmful to the human body due to side effects. Terahertz (THz) trace fingerprint detection is essential because the molecular vibrations of various biological substances such as chiral enantiomers are located in THz range. Recent reported enhanced trace fingerprint technologies have some drawbacks. For instance, multiplexing technology suffered from narrow operation range and limitation by frequency resolution of commercial THz time domain spectroscopy;Absorption induced transparency (AIT) identification for narrowband molecular oscillations suffered from random resonance frequency drift due to fabrication error. In this paper, we proposed frequency-selective fingerprint sensor (FSFS), which can experimentally achieve enhanced trace fingerprint detection by both broadband multiplexing technology and robust AIT identification. Such FSFS is based on polarization independent reconfiguration metasurfaces array. Broadband absorption lines of trace-amount chiral carnitine were boosted with absorption enhancement factors of about 7.3 times based on frequency-selective multiplexing at 0.95-2.0 THz. Enhanced trace narrowband α-lactose fingerprint sensing can be observed at several array structures with absorption enhancement factors of about 7 times based on AIT, exhibiting good robustness. The flexibility and versatility of proposed FSFS has potential applications for boosting trace chiral enantiomer detection as well as diversity of molecular fingerprints identification by both multiplexing and AIT.展开更多
The terahertz(THz)wave is at the intersection between photonics and electronics in the electromagnetic spectrum.Since the vibration mode of many biomedical molecules and the weak interaction mode inside the molecules ...The terahertz(THz)wave is at the intersection between photonics and electronics in the electromagnetic spectrum.Since the vibration mode of many biomedical molecules and the weak interaction mode inside the molecules fall in the THz regime,utilizing THz radiation as a signal source to operate substance information sensing has its unique advantages.Recently,the metamaterial sensor(metasensor)has greatly enhanced the interaction between signal and substances and spectral selectivity on the subwavelength scale.However,most past review articles have demonstrated the THz metasensor in terms of their structures,applications,or materials.Until recently,with the rapid development of metasensing technologies,the molecular information has paid much more attention to the platform of THz metasensors.In this review,we comprehensively introduce the THz metasensor for detecting not only the featureless refractive index but also the vibrational/chiral molecular information of analytes.The objectives of this review are to improve metasensing specificity either by chemical material-assisted analyte capture or by physical molecular information.Later,to boost THz absorption features in a certain frequency,the resonant responses of metasensors can be tuned to the molecular vibrational modes of target molecules,while frequency multiplexing techniques are reviewed to enhance broadband THz spectroscopic fingerprints.The chiral metasensors are also summarized to specific identification chiral molecules.Finally,the potential prospects of next generation THz metasensors are discussed.Compared to featureless refractive index metasensing,the specific metasensor platforms accelerated by material modification and molecular information will lead to greater impact in the advancement of trace detection of conformational dynamics of biomolecules in practical applications.展开更多
Ising machines based on analog systems have the potential to accelerate the solution of ubiquitous combinatorial optimization problems.Although some artificial spins to support large-scale Ising machines have been rep...Ising machines based on analog systems have the potential to accelerate the solution of ubiquitous combinatorial optimization problems.Although some artificial spins to support large-scale Ising machines have been reported,e.g.,superconducting qubits in quantum annealers and short optical pulses in coherent Ising machines,the spin stability is fragile due to the ultra-low equivalent temperature or optical phase sensitivity.In this paper,we propose to use short microwave pulses generated from an optoelectronic parametric oscillator as the spins to implement a large-scale Ising machine with high stability.The proposed machine supports 25,600 spins and can operate continuously and stably for hours.Moreover,the proposed Ising machine is highly compatible with high-speed electronic devices for programmability,paving a low-cost,accurate,and easy-to-implement way toward solving real-world optimization problems.展开更多
Correction: PhotoniX 4, 28 (2023) https://doi.org/10.1186/s43074-023-00108-1 Following publication of the original article [1], the authors reported an error in the spelling of the first author’s last name, the corre...Correction: PhotoniX 4, 28 (2023) https://doi.org/10.1186/s43074-023-00108-1 Following publication of the original article [1], the authors reported an error in the spelling of the first author’s last name, the correct author name is Jiaming Lyu. The original article [1] has been updated.展开更多
In the original publication of this article[1],the video in the additional file 2 was uploaded mistakenly due to a typesetting error,and needs to be updated with the correct one.The original article[1]was updated.
基金supported by the National Natural Science Foundation of China(61905115,62105151,62175109,U21B2033,62105156)Leading Technology of Jiangsu Basic Research Plan(BK20192003),Youth Foundation of Jiangsu Province(BK20190445,BK20210338)+1 种基金Fundamental Research Funds for the Central Universities(30920032101)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201).
文摘Quantitative phase imaging(QPI)has emerged as a valuable tool for biomedical research thanks to its unique capabilities for quantifying optical thickness variation of living cells and tissues.Among many QPI methods,Fourier ptychographic microscopy(FPM)allows long-term label-free observation and quantitative analysis of large cell populations without compromising spatial and temporal resolution.However,high spatio-temporal resolution imaging over a long-time scale(from hours to days)remains a critical challenge:optically inhomogeneous structure of biological specimens as well as mechanical perturbations and thermal fluctuations of the microscope body all result in time-varying aberration and focus drifts,significantly degrading the imaging performance for long-term study.Moreover,the aberrations are sample-and environmentdependent,and cannot be compensated by a fixed optical design,thus necessitating rapid dynamic correction in the imaging process.Here,we report an adaptive optical QPI method based on annular illumination FPM.In this method,the annular matched illumination configuration(i.e.,the illumination numerical aperture(NA)strictly equals to the objective NA),which is the key for recovering low-frequency phase information,is further utilized for the accurate imaging aberration characterization.By using only 6 low-resolution images captured with 6 different illumination angles matching the NA of a 10x,0.4 NA objective,we recover high-resolution quantitative phase images(synthetic NA of 0.8)and characterize the aberrations in real time,restoring the optimum resolution of the system adaptively.Applying our method to live-cell imaging,we achieve diffraction-limited performance(full-pitch resolution of 655 nm at a wavelength of 525 nm)across a wide field of view(1.77mm2)over an extended period of time.
基金Basic Science Center Project of the National Natural Science Foundation of China[Grant No.61988102]National Natural Science Foundation of China[Grant No.62275157]+1 种基金Shanghai Shuguang Program[Grant No.18SG44]111 Project[Grant No.D18014].
文摘Chiral enantiomers have different pharmacological and pharmacokinetic characteristics. It is important to strictly detect chiral component for avoiding being harmful to the human body due to side effects. Terahertz (THz) trace fingerprint detection is essential because the molecular vibrations of various biological substances such as chiral enantiomers are located in THz range. Recent reported enhanced trace fingerprint technologies have some drawbacks. For instance, multiplexing technology suffered from narrow operation range and limitation by frequency resolution of commercial THz time domain spectroscopy;Absorption induced transparency (AIT) identification for narrowband molecular oscillations suffered from random resonance frequency drift due to fabrication error. In this paper, we proposed frequency-selective fingerprint sensor (FSFS), which can experimentally achieve enhanced trace fingerprint detection by both broadband multiplexing technology and robust AIT identification. Such FSFS is based on polarization independent reconfiguration metasurfaces array. Broadband absorption lines of trace-amount chiral carnitine were boosted with absorption enhancement factors of about 7.3 times based on frequency-selective multiplexing at 0.95-2.0 THz. Enhanced trace narrowband α-lactose fingerprint sensing can be observed at several array structures with absorption enhancement factors of about 7 times based on AIT, exhibiting good robustness. The flexibility and versatility of proposed FSFS has potential applications for boosting trace chiral enantiomer detection as well as diversity of molecular fingerprints identification by both multiplexing and AIT.
基金Basic Science Center Project of the National Natural Science Foundation of China(61988102)National Natural Science Foundation of China(62275157)+1 种基金Shanghai Shuguang Program(18SG44)111 Project(D18014)。
文摘The terahertz(THz)wave is at the intersection between photonics and electronics in the electromagnetic spectrum.Since the vibration mode of many biomedical molecules and the weak interaction mode inside the molecules fall in the THz regime,utilizing THz radiation as a signal source to operate substance information sensing has its unique advantages.Recently,the metamaterial sensor(metasensor)has greatly enhanced the interaction between signal and substances and spectral selectivity on the subwavelength scale.However,most past review articles have demonstrated the THz metasensor in terms of their structures,applications,or materials.Until recently,with the rapid development of metasensing technologies,the molecular information has paid much more attention to the platform of THz metasensors.In this review,we comprehensively introduce the THz metasensor for detecting not only the featureless refractive index but also the vibrational/chiral molecular information of analytes.The objectives of this review are to improve metasensing specificity either by chemical material-assisted analyte capture or by physical molecular information.Later,to boost THz absorption features in a certain frequency,the resonant responses of metasensors can be tuned to the molecular vibrational modes of target molecules,while frequency multiplexing techniques are reviewed to enhance broadband THz spectroscopic fingerprints.The chiral metasensors are also summarized to specific identification chiral molecules.Finally,the potential prospects of next generation THz metasensors are discussed.Compared to featureless refractive index metasensing,the specific metasensor platforms accelerated by material modification and molecular information will lead to greater impact in the advancement of trace detection of conformational dynamics of biomolecules in practical applications.
基金supported by the National Key Research and Development Program of China(2021YFB2801804)the National Natural Science Foundation of China(62135014,61925505,62001043)the Key Research Program of the Chinese Academy of Sciences(ZDRW-XX-2022-3,ZDRW-XX-2022-3-1).
文摘Ising machines based on analog systems have the potential to accelerate the solution of ubiquitous combinatorial optimization problems.Although some artificial spins to support large-scale Ising machines have been reported,e.g.,superconducting qubits in quantum annealers and short optical pulses in coherent Ising machines,the spin stability is fragile due to the ultra-low equivalent temperature or optical phase sensitivity.In this paper,we propose to use short microwave pulses generated from an optoelectronic parametric oscillator as the spins to implement a large-scale Ising machine with high stability.The proposed machine supports 25,600 spins and can operate continuously and stably for hours.Moreover,the proposed Ising machine is highly compatible with high-speed electronic devices for programmability,paving a low-cost,accurate,and easy-to-implement way toward solving real-world optimization problems.
文摘Correction: PhotoniX 4, 28 (2023) https://doi.org/10.1186/s43074-023-00108-1 Following publication of the original article [1], the authors reported an error in the spelling of the first author’s last name, the correct author name is Jiaming Lyu. The original article [1] has been updated.
文摘In the original publication of this article[1],the video in the additional file 2 was uploaded mistakenly due to a typesetting error,and needs to be updated with the correct one.The original article[1]was updated.
