Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertilit...Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertility.However,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive.Here,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female infertility.We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic transition.We next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused infertility.We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring.Moreover,those pups grow to adulthood and show normal fertility.Therefore,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation.展开更多
The development of a method for the quantitative determination of the sericin content ratio(SCR)is urgently needed for silk refining and the purification of silk fibroin for biomedical applications.In this work,a seri...The development of a method for the quantitative determination of the sericin content ratio(SCR)is urgently needed for silk refining and the purification of silk fibroin for biomedical applications.In this work,a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk.Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin,and several linear relation-ships of SCR associated with the content ratios of Ser/Ala,Asp/Ala,Lys/Ala,Asp/Gly and Ser/Gly were estab-lished by amino acid analysis.Subsequently,the linear equation expressing SCR(%)as a function of the Asp to Ala content ratio X(%)was established as SCR=2.5634X−12.5587(R 2=0.9972).The results indicated that the SCR of degummed silks calculated by the equation is more objective and effective than the results obtained by the traditional weight loss method.Our study provides a novel approach for the sensitive and quantitative detection of the sericin content within the detection limit in unknown silks,which can contribute to quality control in the silk production process.展开更多
Estuaries are often a significant source of atmospheric CO_(2).However,studies of carbonate systems have predominantly focused on large estuaries,while smaller estuaries have scarcely been documented.In this study,we ...Estuaries are often a significant source of atmospheric CO_(2).However,studies of carbonate systems have predominantly focused on large estuaries,while smaller estuaries have scarcely been documented.In this study,we collected surface and bottom seawater carbonate samples in the subtropical Jiulong River Estuary across different tidal levels from 2019 to 2021.The results showed that estuarine mixing of freshwater from the river with seawater was the dominant factor influencing the estuarine carbonate system.Moreover,estuarine mixing is concomitantly impacted by the net metabolism of biological production and decomposition,groundwater input,release of CO_(2)from the estuary,and precipitation or dissolution of calcium carbonate.The estuarine partial pressure of CO_(2)(pCO_(2))varied from 530μatm to 7715μatm,which represents a strong source of atmospheric CO_(2).The mean annual air-sea CO_(2)flux estimated from three different parameterized equations was approximately(25.63±10.25)mol/(m2·a).Furthermore,the annual emission to the atmosphere was approximately(0.031±0.012)Tg C,which accounts for a mere 0.0077%−0.015%of global estuarine emissions.Dissolved inorganic carbon(DIC),total alkalinity(TA)and the pCO_(2)exhibited high variability throughout the tidal cycle across all cruises.Specifically,the disparities observed between DIC and TA during low and high tides at identical stations during all cruises ranged from approximately 15%to 30%.The variance in the pCO_(2)was even more pronounced,ranging from approximately 30%to 40%.Thus,tidal discrepancies may need to be taken into consideration to estimate the CO_(2)flux from estuarine systems more accurately.展开更多
Due to the proliferation of underwater vehicles and sensors,underwater wireless optical communication(UWOC)is a key enabler for ocean exploration with a strong reliance on short-range bandwidth-intensive communication...Due to the proliferation of underwater vehicles and sensors,underwater wireless optical communication(UWOC)is a key enabler for ocean exploration with a strong reliance on short-range bandwidth-intensive communications.A stable optical link is of primary importance for UWOC.A compact,low-power,and low-cost acquisition,pointing,and tracking(APT)system is proposed and experimentally demonstrated to realign the optical link within 0.04 s,even when the UWOC transmitter and receiver are in relative motion.The system successfully achieves rapid auto-alignment through a 4 m tap water channel with a relatively large number of bubbles.Furthermore,the required minimum illumination value is measured to be as low as7.1 lx,implying that the proposed APT scheme is robust to dim underwater environments.Meanwhile,mobility experiments are performed to verify the performance of the APT system.The proposed system can rapidly and automatically align moving targets in complex and unstable underwater environments,which can potentially boost the practical applications of UWOC.展开更多
基金primarily supported by the Ministry of Science and Technology of the People’s Republic of China(2017YFA0102602,2016YFA0100400)supported by the National Natural Science Foundation of China(81630035,31871448,31721003)+3 种基金the Shanghai Subject Chief Scientist Program(15XD1503500)Supporting Project of Medical Guidance(Western Medicine)of Science and Technology Commission of Shanghai Municipality(15411964600)Merck Serono China Research Fund for Fertility Experts,the Shanghai municipal medical and health discipline construction projects(2017ZZ02015)the Fundamental Research Funds for the Central Universities(1515219049)。
文摘Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertility.However,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive.Here,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female infertility.We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic transition.We next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused infertility.We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring.Moreover,those pups grow to adulthood and show normal fertility.Therefore,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation.
基金supported by the National Key Research and Development Program of China(2017YFC1103602).
文摘The development of a method for the quantitative determination of the sericin content ratio(SCR)is urgently needed for silk refining and the purification of silk fibroin for biomedical applications.In this work,a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk.Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin,and several linear relation-ships of SCR associated with the content ratios of Ser/Ala,Asp/Ala,Lys/Ala,Asp/Gly and Ser/Gly were estab-lished by amino acid analysis.Subsequently,the linear equation expressing SCR(%)as a function of the Asp to Ala content ratio X(%)was established as SCR=2.5634X−12.5587(R 2=0.9972).The results indicated that the SCR of degummed silks calculated by the equation is more objective and effective than the results obtained by the traditional weight loss method.Our study provides a novel approach for the sensitive and quantitative detection of the sericin content within the detection limit in unknown silks,which can contribute to quality control in the silk production process.
基金The Scientific Research Foundation of Third Institute of Oceanography,MNR under contract Nos.2022001,2020017,2023008 and 2019018the Natural Science Foundation of Fujian Province of China under contract No.2023J01209+1 种基金the National Natural Science Foundation of China under contract No.4237061213the Fujian Science and Technology Innovation Leader Project.
文摘Estuaries are often a significant source of atmospheric CO_(2).However,studies of carbonate systems have predominantly focused on large estuaries,while smaller estuaries have scarcely been documented.In this study,we collected surface and bottom seawater carbonate samples in the subtropical Jiulong River Estuary across different tidal levels from 2019 to 2021.The results showed that estuarine mixing of freshwater from the river with seawater was the dominant factor influencing the estuarine carbonate system.Moreover,estuarine mixing is concomitantly impacted by the net metabolism of biological production and decomposition,groundwater input,release of CO_(2)from the estuary,and precipitation or dissolution of calcium carbonate.The estuarine partial pressure of CO_(2)(pCO_(2))varied from 530μatm to 7715μatm,which represents a strong source of atmospheric CO_(2).The mean annual air-sea CO_(2)flux estimated from three different parameterized equations was approximately(25.63±10.25)mol/(m2·a).Furthermore,the annual emission to the atmosphere was approximately(0.031±0.012)Tg C,which accounts for a mere 0.0077%−0.015%of global estuarine emissions.Dissolved inorganic carbon(DIC),total alkalinity(TA)and the pCO_(2)exhibited high variability throughout the tidal cycle across all cruises.Specifically,the disparities observed between DIC and TA during low and high tides at identical stations during all cruises ranged from approximately 15%to 30%.The variance in the pCO_(2)was even more pronounced,ranging from approximately 30%to 40%.Thus,tidal discrepancies may need to be taken into consideration to estimate the CO_(2)flux from estuarine systems more accurately.
基金the National Natural Science Foundation of China(NSFC)(Nos.61971378 and 61671409)National Key Research and DevelopmentProgram of China(Nos.2016YFC1401202,2017YFC0306601,and2017YFC0306100)Strategic Priority Research Program of the ChineseAcademy of Sciences(No.XDA22030208)。
文摘Due to the proliferation of underwater vehicles and sensors,underwater wireless optical communication(UWOC)is a key enabler for ocean exploration with a strong reliance on short-range bandwidth-intensive communications.A stable optical link is of primary importance for UWOC.A compact,low-power,and low-cost acquisition,pointing,and tracking(APT)system is proposed and experimentally demonstrated to realign the optical link within 0.04 s,even when the UWOC transmitter and receiver are in relative motion.The system successfully achieves rapid auto-alignment through a 4 m tap water channel with a relatively large number of bubbles.Furthermore,the required minimum illumination value is measured to be as low as7.1 lx,implying that the proposed APT scheme is robust to dim underwater environments.Meanwhile,mobility experiments are performed to verify the performance of the APT system.The proposed system can rapidly and automatically align moving targets in complex and unstable underwater environments,which can potentially boost the practical applications of UWOC.
