Forster resonance energy transfer(FRET)is a widely used spectroscopic technique.The development of donor-acceptor combinations is essential to advance this technique.To make the energy transfer efficiency independent ...Forster resonance energy transfer(FRET)is a widely used spectroscopic technique.The development of donor-acceptor combinations is essential to advance this technique.To make the energy transfer efficiency independent of the external environment,we explored the potential of constructing donor—acceptor combinations with lanthanide ion-doped nanoparticles(LNPs),in which the 4f electrons of the lanthanide ions are shielded by the 5s and 6p electrons.Non-radiative energy transfer between LNPs was demonstrated in self-assembled nanostructures and core-shell structure of LNPs was used to control the distance between donor and acceptor in self-assembled nanostructures.The emission intensity ratio of donor and acceptor illustrates that the energy transfer efficiency decreases significantly with increasing distance and the effective energy transfer distance is 8.5 nm,showing the potential of using LNPs as donor-acceptor pairs in the construction of distance-dependent energy transfer system.展开更多
Luminescence nanothermometry makes non-invasive and real-time temperature readings possible in living animals.However,the spectral fluctuation in tissues and fluids,as well as the interaction between fluorophores and ...Luminescence nanothermometry makes non-invasive and real-time temperature readings possible in living animals.However,the spectral fluctuation in tissues and fluids,as well as the interaction between fluorophores and environment hinders accuracy of the thermometry.Here,we report a luminescence lifetime-based nanothermometry which specifically addresses this problem.A temporal based calibration(lifetime sensing)in the NIR range,an endogenous thermal response as well as a polymer encapsulation evading environmental factors,altogether help to pinpoint temperature in vivo.Thanks to the highly condensed NdYb ions in a well-protected tiny core-shell nanocrystal(overall 11 nm),a temperature sensitivity about 2.07%K^(-1)(with 5%Yb^(3+)doped nanoparticles)and an accuracy of 0.27 K(with 25%Yb^(3+)doped nanoparticles)in biological fluids are achieved.Hopefully,combining thermally activated energy transfer nanothermometer with anti-interference lifetime thermometry would provide a more accurate temperature measurement for biological and preclinical studies.展开更多
基金Project supported by the National Natural Science Foundation of China(22171054)Shanghai Sci.Tech.Comm.(22XD1420300,21ZR1403900)Innovative Research Team of High-level Local Universities in Shanghai and a key laboratory programof the Education Commission of Shanghai Municipality(ZDSYS14005)。
文摘Forster resonance energy transfer(FRET)is a widely used spectroscopic technique.The development of donor-acceptor combinations is essential to advance this technique.To make the energy transfer efficiency independent of the external environment,we explored the potential of constructing donor—acceptor combinations with lanthanide ion-doped nanoparticles(LNPs),in which the 4f electrons of the lanthanide ions are shielded by the 5s and 6p electrons.Non-radiative energy transfer between LNPs was demonstrated in self-assembled nanostructures and core-shell structure of LNPs was used to control the distance between donor and acceptor in self-assembled nanostructures.The emission intensity ratio of donor and acceptor illustrates that the energy transfer efficiency decreases significantly with increasing distance and the effective energy transfer distance is 8.5 nm,showing the potential of using LNPs as donor-acceptor pairs in the construction of distance-dependent energy transfer system.
基金This work was supported by the National Key R&D Program of China(2017YFA0205100)the National Natural Science Foundation of China(21937003,21527801,21722101).
文摘Luminescence nanothermometry makes non-invasive and real-time temperature readings possible in living animals.However,the spectral fluctuation in tissues and fluids,as well as the interaction between fluorophores and environment hinders accuracy of the thermometry.Here,we report a luminescence lifetime-based nanothermometry which specifically addresses this problem.A temporal based calibration(lifetime sensing)in the NIR range,an endogenous thermal response as well as a polymer encapsulation evading environmental factors,altogether help to pinpoint temperature in vivo.Thanks to the highly condensed NdYb ions in a well-protected tiny core-shell nanocrystal(overall 11 nm),a temperature sensitivity about 2.07%K^(-1)(with 5%Yb^(3+)doped nanoparticles)and an accuracy of 0.27 K(with 25%Yb^(3+)doped nanoparticles)in biological fluids are achieved.Hopefully,combining thermally activated energy transfer nanothermometer with anti-interference lifetime thermometry would provide a more accurate temperature measurement for biological and preclinical studies.
