Oxidative catalysis enables lignin depolymerization to yield carbonyl-containing aromatic chemicals for sustainable lignocellulose valorization.The oxidative depolymerization of lignin requires high oxygen pressure an...Oxidative catalysis enables lignin depolymerization to yield carbonyl-containing aromatic chemicals for sustainable lignocellulose valorization.The oxidative depolymerization of lignin requires high oxygen pressure and harsh conditions to trade off lignin’s structural complexity and limited solubility.Herein,we developed an oxidation system for lignin depolymerization using a single phosphomolybdic acid(H_(3)PMo_(12)O_(40))catalyst in acetic acid solvent to address the aforementioned issues.The entire catalytic system was operated under only 0.1 MPa O_(2) pressure,providing over 20 wt% of aromatic compounds containing aldehydes and carboxylic acids.Theoretical calculations combined with experimental analyses reveal structural transformations and redox behavior driven by the synergistic interaction between H_(3)PMo_(12)O_(40) and acetic acid.Mechanistic studies detected superoxide radicals,confirming the joint role of catalyst and solvent in oxygen activation,radicals stabilization,and enhanced reaction efficiency.A low-cost,commercially available catalyst with minimal oxygen demand offers a promising route to industrial-scale biomass refining.展开更多
The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate thi...The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate this issue,we developed a myristoylation-deficient virus and reporter(luciferase)pseudovirus with a Gly-to-Ala mutation(G2A)on EV71 VP4.When transfecting the EV71-G2 A genome encoding plasmid in cells,the loss of myristoylation on VP4 did not affect the expression of viral proteins and the virus morphology,however,it did significantly influence viral infectivity.Further,in myristoylation-deficient reporter pseudovirus-infected cells,the luciferase activity and viral genome RNA decreased significantly as compared to that of wild type virus;however,cytopathic effect and viral capsid proteins were not detected in myristoylation-deficient virus-infected cells.Also,although myristoylation-deficient viral RNA and proteins were detected in the second blind passage of infection,they were much fewer in number compared to that of the wild type virus.The replication of genomic RNA and negative-strand viral RNA were both blocked in myristoylation-deficient viruses,suggesting that myristoylation affects viral genome RNA release from capsid to cytoplasm.Besides,loss of myristoylation on VP4 altered the distribution of VP4-green fluorescent protein protein,which disappeared from the membrane structure fraction.Finally,a liposome leakage assay showed that EV71 myristoylation mediates the permeability of the model membrane.Hence,the amino-terminal myristoylation of VP4 is pivotal to EV71 infection and capsidmembrane structure interaction.This study provides novel molecular mechanisms regarding EV71 infection and potential molecular targets for antiviral drug design.展开更多
Fault diagnosis of 5G networks faces the challenges of heavy reliance on human experience and insufficient fault samples and relevant monitoring data.The digital twin technology can realize the interaction between vir...Fault diagnosis of 5G networks faces the challenges of heavy reliance on human experience and insufficient fault samples and relevant monitoring data.The digital twin technology can realize the interaction between virtual space and physical space through the fusion of model and data,providing a new paradigm for fault diagnosis.In this paper,we first propose a network digital twin model and apply it to 5G network diagnosis.We then use an improved Average Wasserstein GAN with Gradient Penalty(AWGAN-GP)method to discover and predict failures in the twin network.Finally,we use XGBoost algorithm to locate the faults in physical network in real time.Extensive simulation results show that the proposed approach can significantly increase fault prediction and diagnosis accuracy in the case of a small number of labeled failure samples in 5G networks.展开更多
Three-dimensional reconstruction nerve models are classically obtained from two-dimensional images of ＂visible human＂ frozen sections. However, because of the flexibility of nerve tissues and small color differences...Three-dimensional reconstruction nerve models are classically obtained from two-dimensional images of ＂visible human＂ frozen sections. However, because of the flexibility of nerve tissues and small color differences compared with surrounding tissues, the integrity and validity of nerve tissues can be impaired during milling. Thus, in the present study, we obtained two-dimensional data from a healthy volunteer based on continuous CT angiography and magnetic resonance myelography. Semi-automatic segmentation and reconstruction were then conducted at different thresholds in different tissues using Mimics software. Small anatomical structures such as muscles and cervical nerves were reconstructed using the medical computer aided design module. Three-dimensional digital models of the cervical nerves and their surrounding structures were successfully developed, which allowed visualization of the spatial relation of anatomical structures with a strong three-dimensional effect, distinct appearance, clear distribution, and good continuity, precision, and integrality. These results indicate the validity of a three-dimensional digital visualization model of healthy human cervical nerves, which overcomes the disadvantages of milling, avoids data loss, and exhibits a realistic appearance and three-dimensional image.展开更多
Objective To evaluate the efficacy of early and non-early non-invasive mechanical ventilation(NIV) for acute left heart failure and respiratory failure. Methods 29 cases of patients with acute left heart failure and r...Objective To evaluate the efficacy of early and non-early non-invasive mechanical ventilation(NIV) for acute left heart failure and respiratory failure. Methods 29 cases of patients with acute left heart failure and respiratory failure treated by NIV were selected from our department from August 2016 to March 2019. According to the time of initiation of noninvasive mechanical ventilation, the patients were divided into the early treatment group(group A, treatment with NIV immediately after admission, n=15) and the non-early treatment group(group B, treatment with NIV for 2 h after admission, n=14), the improvement time, mechanical ventilation time, effective rate, intubation rate and fatality rate were compared between the two groups. Results The improvement time of patients in group A was(4.8±2.5) hours, the time of mechanical ventilation was(9.6±3.2) hours, the improvement time of group B was(6.8±2.6) hours, and the time of mechanical ventilation(12.8±4.4) hours. There were significant differences between the two groups(P<0.05). In group A, 13 patients were cured, 2 patients were intubated, 1 patient died, 10 patients in group B were cured, 4 patients were intubated, and 2 patients died. The difference in cure rate, intubation rate and mortality was statistically significant(P<0.05). Conclusion The treatment of acute left heart failure and respiratory failure is effective, and early application can improve the cure rate, reduce intubation and mortality.展开更多
The static coarsening behavior of laser powder directed energy deposited Ti-6.5Al-3.5Mo-1.5Zr-0.3Si titanium alloy and its effects on the alloy’s tensile properties were investigated.The static coarsening kinetics of...The static coarsening behavior of laser powder directed energy deposited Ti-6.5Al-3.5Mo-1.5Zr-0.3Si titanium alloy and its effects on the alloy’s tensile properties were investigated.The static coarsening kinetics of the crab-like primary α(α_(P))and secondary α(α_(s))were satisfied by the Lifshitz-Slyozov-Wagner relationships.For the coarsening of the crab-like𝛼P,the coarsening efficiency(n)was satisfied at 0.25-0.35 for 910℃,whereas it was satisfied at 0.45-0.50 for 950 and 990℃.For the coarsening of the α_(s),the n was satisfied at 0.45-0.50 for 950℃ and 990℃.Compared to the as-built sample,the samples heat-treated at 910℃/2 h/air cooled and 950℃/2 h/air cooled exhibited good yield strength(930-1005.5 MPa),the highest elongation(14.5%-16.6%),and the lowest anisotropic ductility(0.6%)among all samples.The crab-like α_(P) and α_(s) increased the crack growth resistance and decreased the fracture difference in two different directions in the samples.展开更多
Seasonal variations and common mode errors affect the precision of the Global Positioning System(GPS)time series.In this paper,we explore to improve the precision of coordinate time series,thereby providing a better d...Seasonal variations and common mode errors affect the precision of the Global Positioning System(GPS)time series.In this paper,we explore to improve the precision of coordinate time series,thereby providing a better detection of weak or transient deformation signals,particularly co-seismic signals.Based on 97 GPS stations,including the campaign and continuous GPS stations in Nepal and southern Tibet,we first consider seasonal variations and common errors,then obtain co-seismic deformation of the 2015 Gorkha earthquake in Nepal and southern Tibet.Our co-seismic rupture model is characterized by a shallow ramp and a deeper detachment fault,in agreement with the relocated aftershock sequence.Our results indicate that the earthquake rupture is mainly distributed in the upper-crustal fault,and the maximum slip is up to 8.0 m at~15.0 km depth located in the approximate-80 km east of the epicenter.The average slip is more than 5 m,and the total modelled magnitude is M_(W)7.84,consistent with the observed seismic moment.Our rupture model for the 2015 Gorkha earthquake suggests that the rupture zone is not only in the upper crustal Main Himalayan Thrust(MHT),but also spreads to the northern segment of the MHT.展开更多
Low back pain,mainly caused by intervertebral disc degeneration(IVDD),is a common health problem;however,current surgical treatments are less than satisfactory.Thus,it is essential to develop novel non-invasive surgic...Low back pain,mainly caused by intervertebral disc degeneration(IVDD),is a common health problem;however,current surgical treatments are less than satisfactory.Thus,it is essential to develop novel non-invasive surgical methods for IVDD treatment.Here,we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage(ECM-Gels)that are loaded with cartilage endplate stem cells(CESCs).After loaded with CESCs overexpressing Sphk2(Lenti-Sphk2-CESCs)and injected near the cartilage endplate(CEP)of rats in vivo,ECM-Gels produced Sphk2-engineered exosomes(Lenti-Sphk2-Exos).These exosomes penetrated the annulus fibrosus(AF)and transported Sphk2 into the nucleus pulposus cells(NPCs).Sphk2 activated the phosphatidylinositol 3-kinase(PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs,ultimately ameliorating IVDD.This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.展开更多
文摘Oxidative catalysis enables lignin depolymerization to yield carbonyl-containing aromatic chemicals for sustainable lignocellulose valorization.The oxidative depolymerization of lignin requires high oxygen pressure and harsh conditions to trade off lignin’s structural complexity and limited solubility.Herein,we developed an oxidation system for lignin depolymerization using a single phosphomolybdic acid(H_(3)PMo_(12)O_(40))catalyst in acetic acid solvent to address the aforementioned issues.The entire catalytic system was operated under only 0.1 MPa O_(2) pressure,providing over 20 wt% of aromatic compounds containing aldehydes and carboxylic acids.Theoretical calculations combined with experimental analyses reveal structural transformations and redox behavior driven by the synergistic interaction between H_(3)PMo_(12)O_(40) and acetic acid.Mechanistic studies detected superoxide radicals,confirming the joint role of catalyst and solvent in oxygen activation,radicals stabilization,and enhanced reaction efficiency.A low-cost,commercially available catalyst with minimal oxygen demand offers a promising route to industrial-scale biomass refining.
基金supported by the National Natural Science Foundation of China(Grant No.31770184)
文摘The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate this issue,we developed a myristoylation-deficient virus and reporter(luciferase)pseudovirus with a Gly-to-Ala mutation(G2A)on EV71 VP4.When transfecting the EV71-G2 A genome encoding plasmid in cells,the loss of myristoylation on VP4 did not affect the expression of viral proteins and the virus morphology,however,it did significantly influence viral infectivity.Further,in myristoylation-deficient reporter pseudovirus-infected cells,the luciferase activity and viral genome RNA decreased significantly as compared to that of wild type virus;however,cytopathic effect and viral capsid proteins were not detected in myristoylation-deficient virus-infected cells.Also,although myristoylation-deficient viral RNA and proteins were detected in the second blind passage of infection,they were much fewer in number compared to that of the wild type virus.The replication of genomic RNA and negative-strand viral RNA were both blocked in myristoylation-deficient viruses,suggesting that myristoylation affects viral genome RNA release from capsid to cytoplasm.Besides,loss of myristoylation on VP4 altered the distribution of VP4-green fluorescent protein protein,which disappeared from the membrane structure fraction.Finally,a liposome leakage assay showed that EV71 myristoylation mediates the permeability of the model membrane.Hence,the amino-terminal myristoylation of VP4 is pivotal to EV71 infection and capsidmembrane structure interaction.This study provides novel molecular mechanisms regarding EV71 infection and potential molecular targets for antiviral drug design.
基金supported by Natural Science Foundation of China(61871237,92067101)Program to Cultivate Middle-aged and Young Science Leaders of Universities of Jiangsu Province+1 种基金Key R&D plan of Jiangsu Province(BE2021013-3)。
文摘Fault diagnosis of 5G networks faces the challenges of heavy reliance on human experience and insufficient fault samples and relevant monitoring data.The digital twin technology can realize the interaction between virtual space and physical space through the fusion of model and data,providing a new paradigm for fault diagnosis.In this paper,we first propose a network digital twin model and apply it to 5G network diagnosis.We then use an improved Average Wasserstein GAN with Gradient Penalty(AWGAN-GP)method to discover and predict failures in the twin network.Finally,we use XGBoost algorithm to locate the faults in physical network in real time.Extensive simulation results show that the proposed approach can significantly increase fault prediction and diagnosis accuracy in the case of a small number of labeled failure samples in 5G networks.
基金funded by the General Project(class A)of Medical Technology Innovation of Nanjing Military Area in 2011,No.11MA007
文摘Three-dimensional reconstruction nerve models are classically obtained from two-dimensional images of ＂visible human＂ frozen sections. However, because of the flexibility of nerve tissues and small color differences compared with surrounding tissues, the integrity and validity of nerve tissues can be impaired during milling. Thus, in the present study, we obtained two-dimensional data from a healthy volunteer based on continuous CT angiography and magnetic resonance myelography. Semi-automatic segmentation and reconstruction were then conducted at different thresholds in different tissues using Mimics software. Small anatomical structures such as muscles and cervical nerves were reconstructed using the medical computer aided design module. Three-dimensional digital models of the cervical nerves and their surrounding structures were successfully developed, which allowed visualization of the spatial relation of anatomical structures with a strong three-dimensional effect, distinct appearance, clear distribution, and good continuity, precision, and integrality. These results indicate the validity of a three-dimensional digital visualization model of healthy human cervical nerves, which overcomes the disadvantages of milling, avoids data loss, and exhibits a realistic appearance and three-dimensional image.
文摘Objective To evaluate the efficacy of early and non-early non-invasive mechanical ventilation(NIV) for acute left heart failure and respiratory failure. Methods 29 cases of patients with acute left heart failure and respiratory failure treated by NIV were selected from our department from August 2016 to March 2019. According to the time of initiation of noninvasive mechanical ventilation, the patients were divided into the early treatment group(group A, treatment with NIV immediately after admission, n=15) and the non-early treatment group(group B, treatment with NIV for 2 h after admission, n=14), the improvement time, mechanical ventilation time, effective rate, intubation rate and fatality rate were compared between the two groups. Results The improvement time of patients in group A was(4.8±2.5) hours, the time of mechanical ventilation was(9.6±3.2) hours, the improvement time of group B was(6.8±2.6) hours, and the time of mechanical ventilation(12.8±4.4) hours. There were significant differences between the two groups(P<0.05). In group A, 13 patients were cured, 2 patients were intubated, 1 patient died, 10 patients in group B were cured, 4 patients were intubated, and 2 patients died. The difference in cure rate, intubation rate and mortality was statistically significant(P<0.05). Conclusion The treatment of acute left heart failure and respiratory failure is effective, and early application can improve the cure rate, reduce intubation and mortality.
基金supported by National Key Research and Development Program of China(Grant No.2022YFB4602205).
文摘The static coarsening behavior of laser powder directed energy deposited Ti-6.5Al-3.5Mo-1.5Zr-0.3Si titanium alloy and its effects on the alloy’s tensile properties were investigated.The static coarsening kinetics of the crab-like primary α(α_(P))and secondary α(α_(s))were satisfied by the Lifshitz-Slyozov-Wagner relationships.For the coarsening of the crab-like𝛼P,the coarsening efficiency(n)was satisfied at 0.25-0.35 for 910℃,whereas it was satisfied at 0.45-0.50 for 950 and 990℃.For the coarsening of the α_(s),the n was satisfied at 0.45-0.50 for 950℃ and 990℃.Compared to the as-built sample,the samples heat-treated at 910℃/2 h/air cooled and 950℃/2 h/air cooled exhibited good yield strength(930-1005.5 MPa),the highest elongation(14.5%-16.6%),and the lowest anisotropic ductility(0.6%)among all samples.The crab-like α_(P) and α_(s) increased the crack growth resistance and decreased the fracture difference in two different directions in the samples.
基金funded by the Open Fund of Wuhan,Gravitation and Solid Earth Tides,National Observation and Research Station(grant no.WHYWZ202212)the CMONOC project
文摘Seasonal variations and common mode errors affect the precision of the Global Positioning System(GPS)time series.In this paper,we explore to improve the precision of coordinate time series,thereby providing a better detection of weak or transient deformation signals,particularly co-seismic signals.Based on 97 GPS stations,including the campaign and continuous GPS stations in Nepal and southern Tibet,we first consider seasonal variations and common errors,then obtain co-seismic deformation of the 2015 Gorkha earthquake in Nepal and southern Tibet.Our co-seismic rupture model is characterized by a shallow ramp and a deeper detachment fault,in agreement with the relocated aftershock sequence.Our results indicate that the earthquake rupture is mainly distributed in the upper-crustal fault,and the maximum slip is up to 8.0 m at~15.0 km depth located in the approximate-80 km east of the epicenter.The average slip is more than 5 m,and the total modelled magnitude is M_(W)7.84,consistent with the observed seismic moment.Our rupture model for the 2015 Gorkha earthquake suggests that the rupture zone is not only in the upper crustal Main Himalayan Thrust(MHT),but also spreads to the northern segment of the MHT.
基金supported by the National Natural Science Foundation of China(Grant Number:81874028,81702182)the Research Program of Foundation Science and Application Technology of Chongqing(Grant Number:cstc2018jcyjAX0598)Basic Medical College Foundation of Army Medical University(2019JCZX10).
文摘Low back pain,mainly caused by intervertebral disc degeneration(IVDD),is a common health problem;however,current surgical treatments are less than satisfactory.Thus,it is essential to develop novel non-invasive surgical methods for IVDD treatment.Here,we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage(ECM-Gels)that are loaded with cartilage endplate stem cells(CESCs).After loaded with CESCs overexpressing Sphk2(Lenti-Sphk2-CESCs)and injected near the cartilage endplate(CEP)of rats in vivo,ECM-Gels produced Sphk2-engineered exosomes(Lenti-Sphk2-Exos).These exosomes penetrated the annulus fibrosus(AF)and transported Sphk2 into the nucleus pulposus cells(NPCs).Sphk2 activated the phosphatidylinositol 3-kinase(PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs,ultimately ameliorating IVDD.This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.
