Macrophage migration inhibitory factor(MIF),a multifunctional cytokine,is secreted by various cells and participates in inflammatory reactions,including innate and adaptive immunity.There are some evidences that MIF i...Macrophage migration inhibitory factor(MIF),a multifunctional cytokine,is secreted by various cells and participates in inflammatory reactions,including innate and adaptive immunity.There are some evidences that MIF is involved in many vitreoretinal diseases.For example,MIF can exacerbate many types of uveitis;measurements of MIF levels can be used to monitor the effectiveness of uveitis treatment.MIF also alleviates trauma-induced and glaucoma-induced optic nerve damage.Furthermore,MIF is critical for retinal/choroidal neovascularization,especially complex neovascularization.MIF exacerbates retinal degeneration;thus,anti-MIF therapy may help to mitigate retinal degeneration.MIF protects uveal melanoma from attacks by natural killer cells.The mechanism underlying the effects of MIF in these diseases has been demonstrated:it binds to cluster of differentiation 74,inhibits the c-Jun N-terminal kinase pathway,and triggers mitogen-activated protein kinases,extracellular signal-regulated kinase-1/2,and the phosphoinositide-3-kinase/Akt pathway.MIF also upregulates Toll-like receptor 4 and activates the nuclear factor kappa-B signaling pathway.This review focuses on the structure and function of MIF and its receptors,including the effects of MIF on uveal inflammation,retinal degeneration,optic neuropathy,retinal/choroidal neovascularization,and uveal melanoma.展开更多
[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the co...[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.展开更多
[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japon...[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.展开更多
[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm...[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.展开更多
[Objectives] To determine the content of main active flavonoids in the moxa roll and moxa cone from different producing areas and provide a reference for the quality control of finished moxa roll and moxa cone. [Metho...[Objectives] To determine the content of main active flavonoids in the moxa roll and moxa cone from different producing areas and provide a reference for the quality control of finished moxa roll and moxa cone. [Methods]Using HPLC,with kaempferol,jaceosidin and eupatilin as reference substance,there comparative analysis was done on the content of kaempferol,jaceosidin and eupatilin in the moxa roll and moxa cone from different producing areas. [Results]11 lots of samples contained kaempferol,jaceosidin and eupatilin,but the content differed significantly. [Conclusions] There was a large difference in the content of three kinds of flavonoids in the moxa roll and moxa cone from different producing areas,and the study provided a reference for the quality evaluation and quality control of moxa roll and moxa cone.展开更多
基金supported by the Key Program of Natural Science Foundation of Shaanxi Province,No.2021JZ-60(to HZ)。
文摘Macrophage migration inhibitory factor(MIF),a multifunctional cytokine,is secreted by various cells and participates in inflammatory reactions,including innate and adaptive immunity.There are some evidences that MIF is involved in many vitreoretinal diseases.For example,MIF can exacerbate many types of uveitis;measurements of MIF levels can be used to monitor the effectiveness of uveitis treatment.MIF also alleviates trauma-induced and glaucoma-induced optic nerve damage.Furthermore,MIF is critical for retinal/choroidal neovascularization,especially complex neovascularization.MIF exacerbates retinal degeneration;thus,anti-MIF therapy may help to mitigate retinal degeneration.MIF protects uveal melanoma from attacks by natural killer cells.The mechanism underlying the effects of MIF in these diseases has been demonstrated:it binds to cluster of differentiation 74,inhibits the c-Jun N-terminal kinase pathway,and triggers mitogen-activated protein kinases,extracellular signal-regulated kinase-1/2,and the phosphoinositide-3-kinase/Akt pathway.MIF also upregulates Toll-like receptor 4 and activates the nuclear factor kappa-B signaling pathway.This review focuses on the structure and function of MIF and its receptors,including the effects of MIF on uveal inflammation,retinal degeneration,optic neuropathy,retinal/choroidal neovascularization,and uveal melanoma.
文摘[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.
文摘[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.
文摘[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.
文摘[Objectives] To determine the content of main active flavonoids in the moxa roll and moxa cone from different producing areas and provide a reference for the quality control of finished moxa roll and moxa cone. [Methods]Using HPLC,with kaempferol,jaceosidin and eupatilin as reference substance,there comparative analysis was done on the content of kaempferol,jaceosidin and eupatilin in the moxa roll and moxa cone from different producing areas. [Results]11 lots of samples contained kaempferol,jaceosidin and eupatilin,but the content differed significantly. [Conclusions] There was a large difference in the content of three kinds of flavonoids in the moxa roll and moxa cone from different producing areas,and the study provided a reference for the quality evaluation and quality control of moxa roll and moxa cone.
