Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators...Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators of cortical astrocyte maturation.We demonstrate that Olig1 and Olig2 work synergistically to regulate cortical astrocyte maturation by modulating Bmp7 expression.Genetic ablation of both Olig1 and Olig2 results in defective astrocyte morphology,including reduced process complexity and an immature gene expression profile.Single-cell RNA sequencing reveals a shift towards a less mature astrocyte state,marked by elevated levels of HOPX and GFAP,resembling human astrocytes.Mechanistically,Olig1 and Olig2 bind directly to the Bmp7 enhancer,repressing its expression to promote astrocyte maturation.Overexpression of Bmp7 in vivo replicates the astrocyte defects seen in Olig1/2 double mutants,confirming the critical role of BMP7 signaling in this process.These findings provide insights into the transcriptional and signaling pathways regulating astrocyte development and highlight Olig1 and Olig2 as key regulators of cortical astrocyte maturation,with potential implications for understanding glial dysfunction in neurological diseases.展开更多
Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediati...Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediation by the acclimated complex microorganisms of a typical black-odorous stream. After the treatment, the total phosphorus and ammonia contents decreased by 74.0% and 76.3% and the concentrations of dissolved oxygen increased from 1.65 to 4.90 mg/L, indicating the effectiveness of the acclimated composite microorganisms. The proportion of Bacteroidetes decreased significantly by 48.1% and that of Firmicutes increased by 2.23% on average, and the microbial diversity index first increased and then tended to be uniform. Redundancy analysis demonstrated that the pH,dissolved oxygen, and oxidation-reduction potential together determined the composition of the microbial communities(p < 0.05). These findings showed that the acclimated composite microorganisms can effectively remediate the black odor.展开更多
Privacy protection is the key to maintaining the Internet of Things(IoT)communication strategy.Steganography is an important way to achieve covert communication that protects user data privacy.Steganalysis technology ...Privacy protection is the key to maintaining the Internet of Things(IoT)communication strategy.Steganography is an important way to achieve covert communication that protects user data privacy.Steganalysis technology is the key to checking steganography security,and its ultimate goal is to extract embedded messages.Existing methods cannot extract under known cover images.To this end,this paper proposes a method of extracting embedded messages under known cover images.First,the syndrome-trellis encoding process is analyzed.Second,a decoding path in the syndrome trellis is obtained by using the stego sequence and a certain parity-check matrix,while the embedding process is simulated using the cover sequence and parity-check matrix.Since the decoding path obtained by the stego sequence and the correct parity-check matrix is optimal and has the least distortion,comparing the path consistency can quickly filter the coding parameters to determine the correct matrices,and embedded messages can be extracted correctly.The proposed method does not need to embed all possible messages for the second time,improving coding parameter recognition significantly.The experimental results show that the proposed method can identify syndrome-trellis coding parameters in stego images embedded by adaptive steganography quickly to realize embedded message extraction.展开更多
Currently, modeling studies tend to significantly underestimate observed space-based glyoxal （CHOCHO） vertical column densities （VCDs）, implying the existence of missing sources of giyoxal. Several recent studies ...Currently, modeling studies tend to significantly underestimate observed space-based glyoxal （CHOCHO） vertical column densities （VCDs）, implying the existence of missing sources of giyoxal. Several recent studies suggest that the emissions of aromatic compounds and molar yields of glyoxal in the chemical mechanisms may both be underestimated, which can affect the simulated glyoxal concentrations. In this study, the influences of these two factors on glyoxal amounts over China were investigated using the RAMS-CMAQ modeling system for January and July 2014. Four sensitivity simulations were performed, and the results were compared to satellite observations. These results demonstrated significant impacts on glyoxal concentrations from these two factors. In case 1, where the emissions of aromatic compounds were increased three-fold, improvements to glyoxal VCDs were seen in high anthropogenic emissions regions. In case 2, where molar yields of glyoxal from isoprene were increased five-fold, the resulted concentrations in July were B-B-fold higher, achieving closer agreement between the modeled and measured glyoxal VCDs. The combined changes from both cases 1 and 2 were applied in case B, and the model succeeded in further reducing the underestimations of glyoxal VCDs. However, the results over most of the regions with pronounced anthropogenic emissions were still underestimated. So the molar yields of giyoxal from anthropogenic precursors were considered in case 4. With these additional mole yield changes （a two-fold increase）, the improved concentrations agreed better with the measurements in regions of the lower reaches of the Yangtze River and Yellow River in January but not in July.展开更多
The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between...The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between the proton conductivity and the mechanical performance of these materials. Here,we report an effective strategy to fabricate robust high-temperature PEMs based on the in situ electrostatic crosslinking of polyoxometalates and polymers. A comb copolymer poly(ether-ether-ketone)-grafted-poly(2-ethyl-2-oxazoline)(PGE) with transformable side chains was synthesized and complexed with H_(3)PW_(12)O_(40)(PW) by electrostatic self-assembly, forming PGE/PW nanocomposite membranes with bicontinuous nanostructures. After a subsequent PA-treatment of these membranes, high-temperature PEMs of PGE/PW/PA ternary nanocomposites were obtained, in which the in situ electrostatic crosslinking effect between PW and PGE side chains was generated in the hydrophilic domains of the bicontinuous structures. The microphase separation structure and the electrostatic crosslinking feature endow the PGE/PW/PA membranes with excellent anhydrous proton conductive ability while retaining high mechanical performance. The membranes show a high proton conductivity of 42.5 m S/cm at 150 ℃ and a high tensile strength of 13 MPa. Our strategy can pave a new route based on electrostatic control to design nanostructured polymer electrolytes.展开更多
Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model...Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated.The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR.The serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western blot.Results:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model.WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD model.WZR also significantly reduced the expression of pPLC/PLC,pPKC/PKC and CX43.Targeting the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions.Conclusion:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.展开更多
In the present study, we developed and validated a simple and sensitive gradient elution liquid chromatography tandem mass spectrometry （LC-MS/MS） method for quantification of doxorubicin in rabbit plasma. Daunorubi...In the present study, we developed and validated a simple and sensitive gradient elution liquid chromatography tandem mass spectrometry （LC-MS/MS） method for quantification of doxorubicin in rabbit plasma. Daunorubicin was used as an internal standard （IS）. The doxorubicin and IS were extracted with ethyl acetate from plasma samples. The chromatographic separations were achieved on a C18 column （2.1 mm×50 mm, 2.5μm） configured with a C18 guard column （2.1 mm×10 mm, 2.5 μm）. The mobile phase of 0.1% formic acid-water solution and acetonitrile was delivered using a gradient elution program at a flow rate of 0.4 mL/min. The temperature for column was maintained at 40 ℃. The electrospray ionization （ESI） source was operated in the positive ion mode, and the quantification was conducted using multiple reaction monitoring （MRM） of the transitions m/z 544.07→396.96 and m/z 528.06→321.05 for doxorubicin and IS, respectively. The calibration curve of doxorubicin was linear （r 〉 0.999） within the range of 2-600 ng/mL. The lower limit of quantification was 2 ng/mL. The relative errors of intra-day and inter-day accuracies ranged from -2.48% to 0.18% and from -3.78% to 1.94%, respectively. The relative standard deviations of intra-day and inter-day precisions were less than 8.65% and 6.41%, respectively. The method exhibited satisfactory results in terms of specificity, sensitivity, matrix effect, recovery and stability. The newly developed LC-MS/MS method was reliable to monitor doxorubicin concentrations in rabbit plasma.展开更多
The financial market is the core of national economic development,and stocks play an important role in the financial market.Analyzing stock prices has become the focus of investors,analysts,and people in related field...The financial market is the core of national economic development,and stocks play an important role in the financial market.Analyzing stock prices has become the focus of investors,analysts,and people in related fields.This paper evaluates the volatility of Apple Inc.(AAPL)returns using five generalized autoregressive conditional heteroskedasticity(GARCH)models:sGARCH with constant mean,GARCH with sstd,GJR-GARCH,AR(1)GJR-GARCH,and GJR-GARCH in mean.The distribution of AAPL’s closing price and earnings data was analyzed,and skewed student t-distribution(sstd)and normal distribution(norm)were used to further compare the data distribution of the five models and capture the shape,skewness,and loglikelihood in Model 4-AR(1)GJR-GARCH.Through further analysis,the results showed that Model 4,AR(1)GJR-GARCH,is the optimal model to describe the volatility of the return series of AAPL.The analysis of the research process is both,a process of exploration and reflection.By analyzing the stock price of AAPL,we reflect on the shortcomings of previous analysis methods,clarify the purpose of the experiment,and identify the optimal analysis model.展开更多
Magnetic reconnection driving a two-sided-loop jet is typically associated with interactions between an emerging bipole and the overlying horizontal magnetic field,or between filaments from separate magnetic systems.L...Magnetic reconnection driving a two-sided-loop jet is typically associated with interactions between an emerging bipole and the overlying horizontal magnetic field,or between filaments from separate magnetic systems.Leveraging high temporal and spatial resolution observations from ground-based and space-borne instruments,we have identified a two-sided-loop jet originating from magnetic reconnection between threads within a single filament.Our observations show that as two initially crossing filamentary threads within the filament converge,reconnection takes place at their intersection.In the Doppler images,distinct redshift and blueshift signals are observed at the locations where the filament threads intersected.This process generates a two-sided-loop jet with outflow speeds of 22.2 and 62.5 km s^(-1).Following reconnection,the original crossing threads transform into two parallel threads that subsequently separate at speeds of 2.8 and 8.3kms^(-1).This observation offers a new perspective on the mechanisms responsible for jet formation.展开更多
The supply of electricity to remote regions is a significant challenge owing to the pivotal transition in the global energy landscape.To address this issue,an off-grid microgrid solution integrated with energy storage...The supply of electricity to remote regions is a significant challenge owing to the pivotal transition in the global energy landscape.To address this issue,an off-grid microgrid solution integrated with energy storage systems is proposed in this study.Off-grid microgrids are self-sufficient electrical networks that are capable of effectively resolving electricity access problems in remote areas by providing stable and reliable power to local residents.A comprehensive review of the design,control strategies,energy management,and optimization of off-grid microgrids based on domestic and international research is presented in this study.It also explores the critical role of energy stor-age systems in enhancing microgrid stability and economic efficiency.Additionally,the capacity configurations of energy storage systems within off-grid networks are analyzed.Energy storage systems not only mitigate the intermittency and volatility of renewable energy gen-eration but also supply power support during peak demand periods,thereby improving grid stability and reliability.By comparing different energy storage technologies,such as lithium-ion batteries,pumped hydro storage,and compressed air energy storage,the optimal energy storage capacity configurations tailored to various application scenarios are proposed in this study.Finally,using a typical micro-grid as a case study,an empirical analysis of off-grid microgrids and energy storage integration has been conducted.The optimal con-figuration of energy storage systems is determined,and the impact of wind and solar power integration under various scenarios on grid balance is explored.It has been found that a rational configuration of energy storage systems can significantly enhance the utilization rate of renewable energy,reduce system operating costs,and strengthen grid resilience under extreme conditions.This study provides essential theoretical support and practical guidance for the design and implementation of off-grid microgrids in remote areas.展开更多
Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer sc...Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer scale.However,the divergence of the excited Gaussian beam limits the depth-of-field of the system to less than 100μm,which hinders accurate three-dimensional imaging of living tissues and restrictsits applicability in biological research.Therefore,there is an urgent need for an effective method to enhance the depth-of-field without altering the hardware configuration.This paper presents a photoacoustic microscopy depth-of-field extension method and system based on three-dimensional continuity and sparsity deconvolution.This method utilizes a depth-varying point spread function and incorporates continuity and sparsity con-straints into the deconvolution process to mitigate the effect of background noise,enhancing the stability and accuracy of the depth-of-field extension.Experimental results using tungsten wire phantoms suggest that the depth-of-field of system can be extended to 650 pm,which is 7.2 times greater than conventional system,while improving the resolution of the defocused region by an average factor of 3.5.Furthermore,experiments on zebrafish and nude mouse ears with irregular topologies demonstrate that the proposed method successfully overcomes image blurring and the loss of structural information due to limited depth-of-field.All the results suggest that the system with higher lateral resolution and enhanced depth-of-field has significant potential for a wide range of practical biomedical applications.展开更多
In aerospace industry,gears are the most common parts of a mechanical transmission system.Gear pitting faults could cause the transmission system to crash and give rise to safety disaster.It is always a challenging pr...In aerospace industry,gears are the most common parts of a mechanical transmission system.Gear pitting faults could cause the transmission system to crash and give rise to safety disaster.It is always a challenging problem to diagnose the gear pitting condition directly through the raw signal of vibration.In this paper,a novel method named augmented deep sparse autoencoder(ADSAE)is proposed.The method can be used to diagnose the gear pitting fault with relatively few raw vibration signal data.This method is mainly based on the theory of pitting fault diagnosis and creatively combines with both data augmentation ideology and the deep sparse autoencoder algorithm for the fault diagnosis of gear wear.The effectiveness of the proposed method is validated by experiments of six types of gear pitting conditions.The results show that the ADSAE method can effectively increase the network generalization ability and robustness with very high accuracy.This method can effectively diagnose different gear pitting conditions and show the obvious trend according to the severity of gear wear faults.The results obtained by the ADSAE method proposed in this paper are compared with those obtained by other common deep learning methods.This paper provides an important insight into the field of gear fault diagnosis based on deep learning and has a potential practical application value.展开更多
Carrying out green energy transformation,implementing clean energy power replacement and supply,and developing a new power system are some primary driving forces needed to fulfill China’s carbon-peak and carbon-neutr...Carrying out green energy transformation,implementing clean energy power replacement and supply,and developing a new power system are some primary driving forces needed to fulfill China’s carbon-peak and carbon-neutral strategic goals.The construction of new power systems in China’s provinces and cities is developing rapidly,and the lack of a typical model promotes the application.The new power system path design should be based on the actual development of the power grid in different regions,energy use characteristics,and other actual needs to carry out the differentiated path design.In this context,this study analyzes the characteristics of the new domestic power system based on the policy background of the new domestic power system,constructs a new model for power system development stage identification,and proposes the overall design of the new power system development path from the power supply,transmission and distribution,and load sides.It also uses the Hebei South Network as an example to explore the development stage of the Hebei South Grid based on actual development needs.Finally,this study designs a novel power system development path for the entire supply and demand chain for the Hebei South Grid to propose ideas for constructing a new power system in China and to help green energy transformation.展开更多
Although many material designs or strategic methods have been proposed for treating oil spills and oily wastewater,the complex oily state,dealing with the harsh operating conditions of oil–water separation(such as th...Although many material designs or strategic methods have been proposed for treating oil spills and oily wastewater,the complex oily state,dealing with the harsh operating conditions of oil–water separation(such as the recovery of viscous spilled crude oil,bacteria-containing oily wastewater,and removal of spilled oil under fire),and the autorecycling of oil and absorption materials remain a great challenge.This work proposed an ingenious design strategy of“several birds with one stone”to prepare p H/thermoresponsive flame-retardant/photothermal bactericidal P-Fe_(3)O_(4)-polydopamine(PDA)@melamine–formaldehyde(MF)foams.This design makes the foams remarkably effective in the recovery of spilled viscous crude oil as well as in the separation of bacteria-containing oily emulsions,particularly for instant fire extinguishing by magnetically controlled oil absorption as well as for fire alarms.The photothermal effect and p H response induce a change in the surface wettability of the foams,facilitating excellent autoadsorption/desorption of the spilled oil.The photothermal bactericidal activity and fouling resistance of the foam are beneficial to the separation of bacteria-containing oily wastewater.Outstanding flame-retardant properties and maneuverable magnetic control enable the foam to rapidly recover the spilled oil in a large range of fires,extinguish fires instantly,and facilitate early fire warning.The proposed strategy is expected to inspire further research on treating oil spills under complex conditions.展开更多
Flavonols and proanthocyanidins(PAs)are the main pigments in the black spines of cucumber(Cucumis sativus)fruit,and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs.However,in cucumber,the tissue di...Flavonols and proanthocyanidins(PAs)are the main pigments in the black spines of cucumber(Cucumis sativus)fruit,and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs.However,in cucumber,the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by CsMYB60 remain unclear.In this study,we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber.CsMYB60 activated CsFLS and CsLAR by binding to their promoters and directly or indirectly promoted the expression of CsbHLH42,CsMYC1,CsWD40,and CsTATA-box binding protein,resulting in the formation of complexes of these four proteins to increase the expression of Cs4CL and interact with CsTATA-box binding protein to regulate the expression of CsCHS,thereby regulating the biosynthesis of flavonols and PAs in cucumber.Our data provide new insights into the molecular mechanism of flavonoid biosynthesis,which will facilitate molecular breeding to improve fruit quality in cucumber.展开更多
Synechococcus is one of the most abundant picocyanobacteria in marine ecosystem,and the absence of Prochlorococcus would make it indispensable as a primary producer in the Bohai Sea,North China.However,the abundance d...Synechococcus is one of the most abundant picocyanobacteria in marine ecosystem,and the absence of Prochlorococcus would make it indispensable as a primary producer in the Bohai Sea,North China.However,the abundance distribution and genetic diversity of Synechococcus in this region have rarely been reported.In this study,the distribution pattern of Synechococcus abundance was investigated during four cruises in April,June,August,and November from 2018 to 2019,moreover,its phenogenetic diversity was studied based on high-throughput sequencing of the cpe BA operon.The results demonstrate that phycoerythrin-containing Synechococcus was most abundant in August when temperature was high and oxygen saturation was low.During this period,Synechococcus pigment type(PT)2 was abundant in the Bohai Bay and Laizhou Bay under conditions of high nutrient concentration,temperature,and turbidity.In comparison,PT3,especially those clusters characterized with high or variable ratio of phycourobilin and phycoerythrobilin,was predominant in the Bohai Strait and Liaodong Bay under conditions of high salinity,pH,and oxygen saturation.Furthermore,co-occurrence correlations using network analysis revealed that Synechococcus PTs were related to 15.37%-43.48%of the prokaryotic genera.Synechococcus PT3c/PT3d and PT2 were the most important PTs in the network.The hierarchical clustering revealed that taxa co-occurred with Synechococcus PTs diff ered among samples.It could be attributed to the substance exchange and the environmental impact,which calls for more studies in the future.展开更多
This work presents a moving morphable component(MMC)-based framework for solving topology optimization problems considering both single-frequency and band-frequency steady-state structural dynamic responses.In this wo...This work presents a moving morphable component(MMC)-based framework for solving topology optimization problems considering both single-frequency and band-frequency steady-state structural dynamic responses.In this work,a set of morphable components are introduced as the basic building blocks for topology optimization,and the optimized structural layout can be found by optimizing the parameters characterizing the locations and geometries of the components explicitly.The degree of freedom(DOF)elimination technique is also employed to delete unnecessary DOFs at each iteration.Since the proposed approach solves the corresponding optimization problems in an explicit way,some challenging issues(e.g.,the large computational burden related to finite element analysis and sensitivity analysis,the localized eigenmodes in low material density regions,and the impact of excitation frequency on the optimization process)associated with the traditional approaches can be circumvented naturally.Numerical results show that the proposed approach is effective for solving topology optimization problems involving structural dynamic behaviors,especially when high-frequency responses are considered.展开更多
Self-rooted apple stock is widely used for apple production.However,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of China.This is because of the...Self-rooted apple stock is widely used for apple production.However,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of China.This is because of the considerable difference in the development of a gravitropic set-point angle(GSA)between self-rooted apple stock and seedling rootstock.Therefore,it is crucial to study the molecular mechanism of adventitious root GSA in self-rooted apple stock for breeding self-rooted and deep-rooted apple rootstock cultivars.An apple auxin response factor MdARF19 functioned to establish the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.MdARF19 bound directly to the MdPIN7 promoter,activating its transcriptional expression and thus regulating the formation of the adventitious root GSA in 12-2 self-rooted apple stock.However,MdARF19 influenced the expression of auxin efflux carriers(MdPIN3 and MdPIN10)and the establishment of adventitious root GSA of self-rooted apple stock in response to gravity signals by direct activation of MdFLP.Our findings provide new information on the transcriptional regulation of MdPIN7 by auxin response factor MdARF19 in the regulation of the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.展开更多
基金supported by the National Natural Science Foundation of China(82271197,81974175,31820103006,32070971,32200792,32200776).
文摘Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators of cortical astrocyte maturation.We demonstrate that Olig1 and Olig2 work synergistically to regulate cortical astrocyte maturation by modulating Bmp7 expression.Genetic ablation of both Olig1 and Olig2 results in defective astrocyte morphology,including reduced process complexity and an immature gene expression profile.Single-cell RNA sequencing reveals a shift towards a less mature astrocyte state,marked by elevated levels of HOPX and GFAP,resembling human astrocytes.Mechanistically,Olig1 and Olig2 bind directly to the Bmp7 enhancer,repressing its expression to promote astrocyte maturation.Overexpression of Bmp7 in vivo replicates the astrocyte defects seen in Olig1/2 double mutants,confirming the critical role of BMP7 signaling in this process.These findings provide insights into the transcriptional and signaling pathways regulating astrocyte development and highlight Olig1 and Olig2 as key regulators of cortical astrocyte maturation,with potential implications for understanding glial dysfunction in neurological diseases.
基金supported by the Science and Technology program of Yantai (No. 2017ZH095)。
文摘Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediation by the acclimated complex microorganisms of a typical black-odorous stream. After the treatment, the total phosphorus and ammonia contents decreased by 74.0% and 76.3% and the concentrations of dissolved oxygen increased from 1.65 to 4.90 mg/L, indicating the effectiveness of the acclimated composite microorganisms. The proportion of Bacteroidetes decreased significantly by 48.1% and that of Firmicutes increased by 2.23% on average, and the microbial diversity index first increased and then tended to be uniform. Redundancy analysis demonstrated that the pH,dissolved oxygen, and oxidation-reduction potential together determined the composition of the microbial communities(p < 0.05). These findings showed that the acclimated composite microorganisms can effectively remediate the black odor.
基金upported by the National Natural Science Foundation of China(No.U1804263,61772549,62172435)the Zhongyuan Science and Technology Innovation Leading Talent Project(No.214200510019)Thanks to the recommendation of SPDE2020,which gives us the opportunity to publish an expanded and full version of this paper.
文摘Privacy protection is the key to maintaining the Internet of Things(IoT)communication strategy.Steganography is an important way to achieve covert communication that protects user data privacy.Steganalysis technology is the key to checking steganography security,and its ultimate goal is to extract embedded messages.Existing methods cannot extract under known cover images.To this end,this paper proposes a method of extracting embedded messages under known cover images.First,the syndrome-trellis encoding process is analyzed.Second,a decoding path in the syndrome trellis is obtained by using the stego sequence and a certain parity-check matrix,while the embedding process is simulated using the cover sequence and parity-check matrix.Since the decoding path obtained by the stego sequence and the correct parity-check matrix is optimal and has the least distortion,comparing the path consistency can quickly filter the coding parameters to determine the correct matrices,and embedded messages can be extracted correctly.The proposed method does not need to embed all possible messages for the second time,improving coding parameter recognition significantly.The experimental results show that the proposed method can identify syndrome-trellis coding parameters in stego images embedded by adaptive steganography quickly to realize embedded message extraction.
基金supported by the National Natural Science Foundation of China (No. 91544221)the National Key R&D Programs of China (Nos. 2017YFC0209803, 2017YFC0210000)the CAS Strategic Priority Research Program Grant (No. XDB05020000)
文摘Currently, modeling studies tend to significantly underestimate observed space-based glyoxal （CHOCHO） vertical column densities （VCDs）, implying the existence of missing sources of giyoxal. Several recent studies suggest that the emissions of aromatic compounds and molar yields of glyoxal in the chemical mechanisms may both be underestimated, which can affect the simulated glyoxal concentrations. In this study, the influences of these two factors on glyoxal amounts over China were investigated using the RAMS-CMAQ modeling system for January and July 2014. Four sensitivity simulations were performed, and the results were compared to satellite observations. These results demonstrated significant impacts on glyoxal concentrations from these two factors. In case 1, where the emissions of aromatic compounds were increased three-fold, improvements to glyoxal VCDs were seen in high anthropogenic emissions regions. In case 2, where molar yields of glyoxal from isoprene were increased five-fold, the resulted concentrations in July were B-B-fold higher, achieving closer agreement between the modeled and measured glyoxal VCDs. The combined changes from both cases 1 and 2 were applied in case B, and the model succeeded in further reducing the underestimations of glyoxal VCDs. However, the results over most of the regions with pronounced anthropogenic emissions were still underestimated. So the molar yields of giyoxal from anthropogenic precursors were considered in case 4. With these additional mole yield changes （a two-fold increase）, the improved concentrations agreed better with the measurements in regions of the lower reaches of the Yangtze River and Yellow River in January but not in July.
基金financial support from the National Natural Science Foundation of China (No. 22075097)the Program for JLU Science and Technology Innovative Research Team (No. 2017TD-10)the Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (No. 2020–09)。
文摘The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between the proton conductivity and the mechanical performance of these materials. Here,we report an effective strategy to fabricate robust high-temperature PEMs based on the in situ electrostatic crosslinking of polyoxometalates and polymers. A comb copolymer poly(ether-ether-ketone)-grafted-poly(2-ethyl-2-oxazoline)(PGE) with transformable side chains was synthesized and complexed with H_(3)PW_(12)O_(40)(PW) by electrostatic self-assembly, forming PGE/PW nanocomposite membranes with bicontinuous nanostructures. After a subsequent PA-treatment of these membranes, high-temperature PEMs of PGE/PW/PA ternary nanocomposites were obtained, in which the in situ electrostatic crosslinking effect between PW and PGE side chains was generated in the hydrophilic domains of the bicontinuous structures. The microphase separation structure and the electrostatic crosslinking feature endow the PGE/PW/PA membranes with excellent anhydrous proton conductive ability while retaining high mechanical performance. The membranes show a high proton conductivity of 42.5 m S/cm at 150 ℃ and a high tensile strength of 13 MPa. Our strategy can pave a new route based on electrostatic control to design nanostructured polymer electrolytes.
基金supported by Beijing University of Chinese Medicine(1000061224003)National Natural Science Foundation of China(81903766,81900603,82104440)。
文摘Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated.The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR.The serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western blot.Results:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model.WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD model.WZR also significantly reduced the expression of pPLC/PLC,pPKC/PKC and CX43.Targeting the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions.Conclusion:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.
基金National Natural Science Foundation of China（ Grant No.81571779）.
文摘In the present study, we developed and validated a simple and sensitive gradient elution liquid chromatography tandem mass spectrometry （LC-MS/MS） method for quantification of doxorubicin in rabbit plasma. Daunorubicin was used as an internal standard （IS）. The doxorubicin and IS were extracted with ethyl acetate from plasma samples. The chromatographic separations were achieved on a C18 column （2.1 mm×50 mm, 2.5μm） configured with a C18 guard column （2.1 mm×10 mm, 2.5 μm）. The mobile phase of 0.1% formic acid-water solution and acetonitrile was delivered using a gradient elution program at a flow rate of 0.4 mL/min. The temperature for column was maintained at 40 ℃. The electrospray ionization （ESI） source was operated in the positive ion mode, and the quantification was conducted using multiple reaction monitoring （MRM） of the transitions m/z 544.07→396.96 and m/z 528.06→321.05 for doxorubicin and IS, respectively. The calibration curve of doxorubicin was linear （r 〉 0.999） within the range of 2-600 ng/mL. The lower limit of quantification was 2 ng/mL. The relative errors of intra-day and inter-day accuracies ranged from -2.48% to 0.18% and from -3.78% to 1.94%, respectively. The relative standard deviations of intra-day and inter-day precisions were less than 8.65% and 6.41%, respectively. The method exhibited satisfactory results in terms of specificity, sensitivity, matrix effect, recovery and stability. The newly developed LC-MS/MS method was reliable to monitor doxorubicin concentrations in rabbit plasma.
文摘The financial market is the core of national economic development,and stocks play an important role in the financial market.Analyzing stock prices has become the focus of investors,analysts,and people in related fields.This paper evaluates the volatility of Apple Inc.(AAPL)returns using five generalized autoregressive conditional heteroskedasticity(GARCH)models:sGARCH with constant mean,GARCH with sstd,GJR-GARCH,AR(1)GJR-GARCH,and GJR-GARCH in mean.The distribution of AAPL’s closing price and earnings data was analyzed,and skewed student t-distribution(sstd)and normal distribution(norm)were used to further compare the data distribution of the five models and capture the shape,skewness,and loglikelihood in Model 4-AR(1)GJR-GARCH.Through further analysis,the results showed that Model 4,AR(1)GJR-GARCH,is the optimal model to describe the volatility of the return series of AAPL.The analysis of the research process is both,a process of exploration and reflection.By analyzing the stock price of AAPL,we reflect on the shortcomings of previous analysis methods,clarify the purpose of the experiment,and identify the optimal analysis model.
基金supported by the National Natural Science Foundation of China(NSFC,grant Nos.12303062 and 12203043)the Sichuan Science and Technology Program(2023NSFSC1351,2025ZNSFSC0315)+1 种基金Key Laboratory of Detection and Application of Space Effect in Southwest Sichuan at Leshan Normal University,Education Department of Sichuan Province(No.ZDXM20241002)Project Supported by the Specialized Research Fund for State Key Laboratory of Solar Activity and Space Weather。
文摘Magnetic reconnection driving a two-sided-loop jet is typically associated with interactions between an emerging bipole and the overlying horizontal magnetic field,or between filaments from separate magnetic systems.Leveraging high temporal and spatial resolution observations from ground-based and space-borne instruments,we have identified a two-sided-loop jet originating from magnetic reconnection between threads within a single filament.Our observations show that as two initially crossing filamentary threads within the filament converge,reconnection takes place at their intersection.In the Doppler images,distinct redshift and blueshift signals are observed at the locations where the filament threads intersected.This process generates a two-sided-loop jet with outflow speeds of 22.2 and 62.5 km s^(-1).Following reconnection,the original crossing threads transform into two parallel threads that subsequently separate at speeds of 2.8 and 8.3kms^(-1).This observation offers a new perspective on the mechanisms responsible for jet formation.
基金funded by Humanities and Social Sciences of Ministry of Education Planning Fund of China(21YJA790009)National Natural Science Foundation of China(72140001).
文摘The supply of electricity to remote regions is a significant challenge owing to the pivotal transition in the global energy landscape.To address this issue,an off-grid microgrid solution integrated with energy storage systems is proposed in this study.Off-grid microgrids are self-sufficient electrical networks that are capable of effectively resolving electricity access problems in remote areas by providing stable and reliable power to local residents.A comprehensive review of the design,control strategies,energy management,and optimization of off-grid microgrids based on domestic and international research is presented in this study.It also explores the critical role of energy stor-age systems in enhancing microgrid stability and economic efficiency.Additionally,the capacity configurations of energy storage systems within off-grid networks are analyzed.Energy storage systems not only mitigate the intermittency and volatility of renewable energy gen-eration but also supply power support during peak demand periods,thereby improving grid stability and reliability.By comparing different energy storage technologies,such as lithium-ion batteries,pumped hydro storage,and compressed air energy storage,the optimal energy storage capacity configurations tailored to various application scenarios are proposed in this study.Finally,using a typical micro-grid as a case study,an empirical analysis of off-grid microgrids and energy storage integration has been conducted.The optimal con-figuration of energy storage systems is determined,and the impact of wind and solar power integration under various scenarios on grid balance is explored.It has been found that a rational configuration of energy storage systems can significantly enhance the utilization rate of renewable energy,reduce system operating costs,and strengthen grid resilience under extreme conditions.This study provides essential theoretical support and practical guidance for the design and implementation of off-grid microgrids in remote areas.
基金supported by the National Key R&D Program of China[Grant No.2022YFC2402400]the National Natural Science Foundation of China[Grant No.62275062]+2 种基金Project of Shandong Innovation and Startup Community of High-end Medical Apparatus and Instruments[Grant Nos.2023-SGTTXM-002 and 2024-SGTTXM-005]the Shandong Province Technology Innovation Guidance Plan(Central Leading Local Science and Technology Development Fund)[Grant No.YDZX2023115]the Taishan Scholar Special Funding Project of Shandong Province,and the Shandong Laboratory of Advanced Biomaterials and Medical Devices in Weihai[Grant No.ZL202402].
文摘Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer scale.However,the divergence of the excited Gaussian beam limits the depth-of-field of the system to less than 100μm,which hinders accurate three-dimensional imaging of living tissues and restrictsits applicability in biological research.Therefore,there is an urgent need for an effective method to enhance the depth-of-field without altering the hardware configuration.This paper presents a photoacoustic microscopy depth-of-field extension method and system based on three-dimensional continuity and sparsity deconvolution.This method utilizes a depth-varying point spread function and incorporates continuity and sparsity con-straints into the deconvolution process to mitigate the effect of background noise,enhancing the stability and accuracy of the depth-of-field extension.Experimental results using tungsten wire phantoms suggest that the depth-of-field of system can be extended to 650 pm,which is 7.2 times greater than conventional system,while improving the resolution of the defocused region by an average factor of 3.5.Furthermore,experiments on zebrafish and nude mouse ears with irregular topologies demonstrate that the proposed method successfully overcomes image blurring and the loss of structural information due to limited depth-of-field.All the results suggest that the system with higher lateral resolution and enhanced depth-of-field has significant potential for a wide range of practical biomedical applications.
基金supported by the Natural Science Foundation of China(No.51675089).
文摘In aerospace industry,gears are the most common parts of a mechanical transmission system.Gear pitting faults could cause the transmission system to crash and give rise to safety disaster.It is always a challenging problem to diagnose the gear pitting condition directly through the raw signal of vibration.In this paper,a novel method named augmented deep sparse autoencoder(ADSAE)is proposed.The method can be used to diagnose the gear pitting fault with relatively few raw vibration signal data.This method is mainly based on the theory of pitting fault diagnosis and creatively combines with both data augmentation ideology and the deep sparse autoencoder algorithm for the fault diagnosis of gear wear.The effectiveness of the proposed method is validated by experiments of six types of gear pitting conditions.The results show that the ADSAE method can effectively increase the network generalization ability and robustness with very high accuracy.This method can effectively diagnose different gear pitting conditions and show the obvious trend according to the severity of gear wear faults.The results obtained by the ADSAE method proposed in this paper are compared with those obtained by other common deep learning methods.This paper provides an important insight into the field of gear fault diagnosis based on deep learning and has a potential practical application value.
基金funded by the State Grid Hebei Electric Power Co.,Ltd projectthe National Natural Science Foundation of China’s major project,“Research on the Construction of China’s Economic Transformation Mode for Carbon Neutrality(72140001)This study is titled“Research on Novel Power System Development Path”。
文摘Carrying out green energy transformation,implementing clean energy power replacement and supply,and developing a new power system are some primary driving forces needed to fulfill China’s carbon-peak and carbon-neutral strategic goals.The construction of new power systems in China’s provinces and cities is developing rapidly,and the lack of a typical model promotes the application.The new power system path design should be based on the actual development of the power grid in different regions,energy use characteristics,and other actual needs to carry out the differentiated path design.In this context,this study analyzes the characteristics of the new domestic power system based on the policy background of the new domestic power system,constructs a new model for power system development stage identification,and proposes the overall design of the new power system development path from the power supply,transmission and distribution,and load sides.It also uses the Hebei South Network as an example to explore the development stage of the Hebei South Grid based on actual development needs.Finally,this study designs a novel power system development path for the entire supply and demand chain for the Hebei South Grid to propose ideas for constructing a new power system in China and to help green energy transformation.
基金financially supported by the National Natural Science Foundation of China(No.22078077)the National Science Foundation of Guangdong Province(No.2021A1515010078)financial support of Taif University Researchers Supporting Project(No.TURSP-2020/14),Taif University,Taif,Saudi Arabia。
文摘Although many material designs or strategic methods have been proposed for treating oil spills and oily wastewater,the complex oily state,dealing with the harsh operating conditions of oil–water separation(such as the recovery of viscous spilled crude oil,bacteria-containing oily wastewater,and removal of spilled oil under fire),and the autorecycling of oil and absorption materials remain a great challenge.This work proposed an ingenious design strategy of“several birds with one stone”to prepare p H/thermoresponsive flame-retardant/photothermal bactericidal P-Fe_(3)O_(4)-polydopamine(PDA)@melamine–formaldehyde(MF)foams.This design makes the foams remarkably effective in the recovery of spilled viscous crude oil as well as in the separation of bacteria-containing oily emulsions,particularly for instant fire extinguishing by magnetically controlled oil absorption as well as for fire alarms.The photothermal effect and p H response induce a change in the surface wettability of the foams,facilitating excellent autoadsorption/desorption of the spilled oil.The photothermal bactericidal activity and fouling resistance of the foam are beneficial to the separation of bacteria-containing oily wastewater.Outstanding flame-retardant properties and maneuverable magnetic control enable the foam to rapidly recover the spilled oil in a large range of fires,extinguish fires instantly,and facilitate early fire warning.The proposed strategy is expected to inspire further research on treating oil spills under complex conditions.
基金supported by fundings from the National Natural Science Foundation of China(31672170 and 31872950)the Natural Science Foundation of Shandong Province(JQ201309)+1 种基金the Shandong“Double Tops”Program(SYL2017YSTD06)the“Taishan Scholar”Foundation of the People’s Government of Shandong Province(ts20130932).
文摘Flavonols and proanthocyanidins(PAs)are the main pigments in the black spines of cucumber(Cucumis sativus)fruit,and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs.However,in cucumber,the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by CsMYB60 remain unclear.In this study,we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber.CsMYB60 activated CsFLS and CsLAR by binding to their promoters and directly or indirectly promoted the expression of CsbHLH42,CsMYC1,CsWD40,and CsTATA-box binding protein,resulting in the formation of complexes of these four proteins to increase the expression of Cs4CL and interact with CsTATA-box binding protein to regulate the expression of CsCHS,thereby regulating the biosynthesis of flavonols and PAs in cucumber.Our data provide new insights into the molecular mechanism of flavonoid biosynthesis,which will facilitate molecular breeding to improve fruit quality in cucumber.
基金Supported by the Key Deployment Project of Centre for Ocean Mega-Research of Science,Chinese Academy of Sciences(No.COMS2020Q09)the National Key Research and Development Program of China(No.2018YFD0901102)the Science and Technology program of Yantai(No.2017ZH095)。
文摘Synechococcus is one of the most abundant picocyanobacteria in marine ecosystem,and the absence of Prochlorococcus would make it indispensable as a primary producer in the Bohai Sea,North China.However,the abundance distribution and genetic diversity of Synechococcus in this region have rarely been reported.In this study,the distribution pattern of Synechococcus abundance was investigated during four cruises in April,June,August,and November from 2018 to 2019,moreover,its phenogenetic diversity was studied based on high-throughput sequencing of the cpe BA operon.The results demonstrate that phycoerythrin-containing Synechococcus was most abundant in August when temperature was high and oxygen saturation was low.During this period,Synechococcus pigment type(PT)2 was abundant in the Bohai Bay and Laizhou Bay under conditions of high nutrient concentration,temperature,and turbidity.In comparison,PT3,especially those clusters characterized with high or variable ratio of phycourobilin and phycoerythrobilin,was predominant in the Bohai Strait and Liaodong Bay under conditions of high salinity,pH,and oxygen saturation.Furthermore,co-occurrence correlations using network analysis revealed that Synechococcus PTs were related to 15.37%-43.48%of the prokaryotic genera.Synechococcus PT3c/PT3d and PT2 were the most important PTs in the network.The hierarchical clustering revealed that taxa co-occurred with Synechococcus PTs diff ered among samples.It could be attributed to the substance exchange and the environmental impact,which calls for more studies in the future.
基金Financial support from the National Natural Science Foundation of China (11821202,11872141,11922204,12002073)the National Key Research and Development Plan (2020YFB1709401)+1 种基金the Fundamental Research Funds for the Central Universities[DUT20RC (3)020]the 111 Project (B14013)is gratefully acknowledged.
文摘This work presents a moving morphable component(MMC)-based framework for solving topology optimization problems considering both single-frequency and band-frequency steady-state structural dynamic responses.In this work,a set of morphable components are introduced as the basic building blocks for topology optimization,and the optimized structural layout can be found by optimizing the parameters characterizing the locations and geometries of the components explicitly.The degree of freedom(DOF)elimination technique is also employed to delete unnecessary DOFs at each iteration.Since the proposed approach solves the corresponding optimization problems in an explicit way,some challenging issues(e.g.,the large computational burden related to finite element analysis and sensitivity analysis,the localized eigenmodes in low material density regions,and the impact of excitation frequency on the optimization process)associated with the traditional approaches can be circumvented naturally.Numerical results show that the proposed approach is effective for solving topology optimization problems involving structural dynamic behaviors,especially when high-frequency responses are considered.
基金the National Natural Science Foundation of China(Grant Nos.32102310,32202484,and 32072520)the Shandong Key Research and Development Program,China(Grant Nos.2021LZGC007 and 2022TZXD009).
文摘Self-rooted apple stock is widely used for apple production.However,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of China.This is because of the considerable difference in the development of a gravitropic set-point angle(GSA)between self-rooted apple stock and seedling rootstock.Therefore,it is crucial to study the molecular mechanism of adventitious root GSA in self-rooted apple stock for breeding self-rooted and deep-rooted apple rootstock cultivars.An apple auxin response factor MdARF19 functioned to establish the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.MdARF19 bound directly to the MdPIN7 promoter,activating its transcriptional expression and thus regulating the formation of the adventitious root GSA in 12-2 self-rooted apple stock.However,MdARF19 influenced the expression of auxin efflux carriers(MdPIN3 and MdPIN10)and the establishment of adventitious root GSA of self-rooted apple stock in response to gravity signals by direct activation of MdFLP.Our findings provide new information on the transcriptional regulation of MdPIN7 by auxin response factor MdARF19 in the regulation of the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.
