In a recent study published in Nature by Chen et al.,six novel cryo-EM structures of atypical chemokine receptor 3(ACKR3)complexes with Arrestin2(Arr2,also known asβ-arrestin1)and Arrestin3(Arr3,also known asβ-arres...In a recent study published in Nature by Chen et al.,six novel cryo-EM structures of atypical chemokine receptor 3(ACKR3)complexes with Arrestin2(Arr2,also known asβ-arrestin1)and Arrestin3(Arr3,also known asβ-arrestin2)were resolved using a novel nanobody,Fab7,which stabilizes active arrestin independent of the isoform,interacting receptor or its phosphorylation pattern.1 This work provides critical insights into G protein-coupled receptor(GPCR)–arrestin interactions under specific GPCR kinase(GRK)phosphorylation conditions,allowing an unprecedented direct comparison of these dynamic signaling complexes.展开更多
文摘In a recent study published in Nature by Chen et al.,six novel cryo-EM structures of atypical chemokine receptor 3(ACKR3)complexes with Arrestin2(Arr2,also known asβ-arrestin1)and Arrestin3(Arr3,also known asβ-arrestin2)were resolved using a novel nanobody,Fab7,which stabilizes active arrestin independent of the isoform,interacting receptor or its phosphorylation pattern.1 This work provides critical insights into G protein-coupled receptor(GPCR)–arrestin interactions under specific GPCR kinase(GRK)phosphorylation conditions,allowing an unprecedented direct comparison of these dynamic signaling complexes.
