The broad trans-sectoral(humans,animals,environment)dissemination potential of OXA-48 carbapenemase-producing Escherichia coli(OXA-48-Ec)and OXA-48-harboring plasmids represent a major One-Health concern.A wholegenome...The broad trans-sectoral(humans,animals,environment)dissemination potential of OXA-48 carbapenemase-producing Escherichia coli(OXA-48-Ec)and OXA-48-harboring plasmids represent a major One-Health concern.A wholegenome sequence analysis was conducted with 75 OXA-48-Ec isolated between 2018 and 2022 from dogs(n=13)and cats(n=4)hospitalized in a veterinary clinic,the environment of another veterinary clinic(n=2),humans(n=55),and river water(n=1).Strain sequence types(ST),core-genome MLST-based complex type(CT),antimicrobial resistance genes,plasmid incompatibility(Inc)groups were determined by Illumina sequence analysis.Animal OXA-48-Ec and their respective plasmids as well as representative human strains harboring similar OXA-48 plasmids as those from animals were also sequenced using Oxford Nanopore Technologies for comparative analysis of complete plasmids.The 19 veterinary strains belonged to 13 STs,and the 55 human strains to 25 different STs.Only two animal strains shared the same ST as the human strains(ST58,ST405),but with different CTs.Strains carried bla_(OXA-48)mainly on IncL-type plasmids(n=35),except some non-veterinary strains,where the gene was located on Col156-like plasmids(human,n=14;water,n=1),on the chromosome(n=7)or onto yet-uncharacterized elements(n=18)(humans).Two ST58 animal strains harbored bla_(OXA-48)onto a novel 76-kb IncL-type plasmid also harboring the sul1 and blaDHA-1 resistance genes.Our analysis showed a high genetic diversity between OXA-48-Ec deriving from the human and small animal clinical settings.However,diverse strains from all isolation sources harbored highly similar bla_(OXA-48)-carrying IncL plasmids,underscoring the potential contribution of shared ecological niches in the spread of this hyperepidemic plasmid.展开更多
基金funded by Grant no.1.21.07 of the Swiss Federal Food Safety and Veterinary Office FSVO"Whole-genome-sequencing of cephalosporinaseand carbapenemase-producing Enterobacteriaceae from animals:a baseline for a One health molecular epidemiology"to V.P.
文摘The broad trans-sectoral(humans,animals,environment)dissemination potential of OXA-48 carbapenemase-producing Escherichia coli(OXA-48-Ec)and OXA-48-harboring plasmids represent a major One-Health concern.A wholegenome sequence analysis was conducted with 75 OXA-48-Ec isolated between 2018 and 2022 from dogs(n=13)and cats(n=4)hospitalized in a veterinary clinic,the environment of another veterinary clinic(n=2),humans(n=55),and river water(n=1).Strain sequence types(ST),core-genome MLST-based complex type(CT),antimicrobial resistance genes,plasmid incompatibility(Inc)groups were determined by Illumina sequence analysis.Animal OXA-48-Ec and their respective plasmids as well as representative human strains harboring similar OXA-48 plasmids as those from animals were also sequenced using Oxford Nanopore Technologies for comparative analysis of complete plasmids.The 19 veterinary strains belonged to 13 STs,and the 55 human strains to 25 different STs.Only two animal strains shared the same ST as the human strains(ST58,ST405),but with different CTs.Strains carried bla_(OXA-48)mainly on IncL-type plasmids(n=35),except some non-veterinary strains,where the gene was located on Col156-like plasmids(human,n=14;water,n=1),on the chromosome(n=7)or onto yet-uncharacterized elements(n=18)(humans).Two ST58 animal strains harbored bla_(OXA-48)onto a novel 76-kb IncL-type plasmid also harboring the sul1 and blaDHA-1 resistance genes.Our analysis showed a high genetic diversity between OXA-48-Ec deriving from the human and small animal clinical settings.However,diverse strains from all isolation sources harbored highly similar bla_(OXA-48)-carrying IncL plasmids,underscoring the potential contribution of shared ecological niches in the spread of this hyperepidemic plasmid.
