Objective:Based on network pharmacology and molecular docking,we want to explore the pharmacological effects and mechanism of Danggui Sini Decoction in the treatment of lower extremity arteriosclerosis obliterans.Meth...Objective:Based on network pharmacology and molecular docking,we want to explore the pharmacological effects and mechanism of Danggui Sini Decoction in the treatment of lower extremity arteriosclerosis obliterans.Methods:Using TCMSP database to search the main active components of Danggui Sini Decoction(当归四逆汤,DSD)and their related targets,Cytoscape3.8.1 software was used to construct a"component-disease-target"interaction network.Meanwhile,DAVID database was used to enrich the key target genes with GO and KEGG functions.And we used Auto Dock Tools 1.5.6 for molecular docking.Results:A total of 45 candidate active molecules and 250 potential target proteins related to ASO were screened.Key genes such as TNF,IL6,VEGFA,MMP9,IL1B,CCL2,CXCL8,ICAM1,VCAM1,and IL10 are mainly through TNF signaling pathway,HIF-1 signaling pathway,cytokine-cytokine receptor interaction,Toll-like receptor signaling pathway,NF-kappa B signaling pathway,MAPK signaling pathway and other biological pathways exert their effects.The results of molecular docking showed that 5UUI-sesamin,5UUI-paeoniflorin,4XCT-sesamin,and 4XCT-sesamin have extremely strong binding ability.Conclusion:Danggui Sini Decoction(当归四逆汤,DSD)can synergistically exert pharmacological effects through multiple components,multiple targets,and multiple pathways.On the basis of regulating immunity and inhibiting smooth muscle proliferation,it can prevent the occurrence and development of ASO.展开更多
The black cubic boron nitride(cBN)single crystal is synthesized by using hBN-LiH and hBN-Li_(3)N-B as the raw materials at high temperature and high pressure(HTHP).The colors of the cBN crystal synthesized in an hBN-L...The black cubic boron nitride(cBN)single crystal is synthesized by using hBN-LiH and hBN-Li_(3)N-B as the raw materials at high temperature and high pressure(HTHP).The colors of the cBN crystal synthesized in an hBN-Li_(3)N-B system vary from transparent yellow,half-transparent and then opaque black with the increasing B content in the raw materials.It is worth noting that a trigonal shadow is presented at the center of the cBN crystal synthesized in the hBN-Li_(3)N-B system but can not be found in the hBN-LiH system.Analyzing the Raman spectrum,we find that the darkening and the trigonal shadow in the cBN crystal may be due to the presence of excess B atoms.The above-mentioned phenomenon can be determined by removing impurity capacity and growth environment of the cBN crystal.展开更多
基金supported by Shanxi Provincial Department of Science and Technology Applied Basic Research Project(201901D211537)Shanxi University of Traditional Chinese Medicine Science and Technology Innovation Capability Cultivation Project(2019PY-007)Shanxi Province Zheng Xuejun's famous traditional Chinese medicine expert inheritance work(SXSMZYGZS023).
文摘Objective:Based on network pharmacology and molecular docking,we want to explore the pharmacological effects and mechanism of Danggui Sini Decoction in the treatment of lower extremity arteriosclerosis obliterans.Methods:Using TCMSP database to search the main active components of Danggui Sini Decoction(当归四逆汤,DSD)and their related targets,Cytoscape3.8.1 software was used to construct a"component-disease-target"interaction network.Meanwhile,DAVID database was used to enrich the key target genes with GO and KEGG functions.And we used Auto Dock Tools 1.5.6 for molecular docking.Results:A total of 45 candidate active molecules and 250 potential target proteins related to ASO were screened.Key genes such as TNF,IL6,VEGFA,MMP9,IL1B,CCL2,CXCL8,ICAM1,VCAM1,and IL10 are mainly through TNF signaling pathway,HIF-1 signaling pathway,cytokine-cytokine receptor interaction,Toll-like receptor signaling pathway,NF-kappa B signaling pathway,MAPK signaling pathway and other biological pathways exert their effects.The results of molecular docking showed that 5UUI-sesamin,5UUI-paeoniflorin,4XCT-sesamin,and 4XCT-sesamin have extremely strong binding ability.Conclusion:Danggui Sini Decoction(当归四逆汤,DSD)can synergistically exert pharmacological effects through multiple components,multiple targets,and multiple pathways.On the basis of regulating immunity and inhibiting smooth muscle proliferation,it can prevent the occurrence and development of ASO.
文摘The black cubic boron nitride(cBN)single crystal is synthesized by using hBN-LiH and hBN-Li_(3)N-B as the raw materials at high temperature and high pressure(HTHP).The colors of the cBN crystal synthesized in an hBN-Li_(3)N-B system vary from transparent yellow,half-transparent and then opaque black with the increasing B content in the raw materials.It is worth noting that a trigonal shadow is presented at the center of the cBN crystal synthesized in the hBN-Li_(3)N-B system but can not be found in the hBN-LiH system.Analyzing the Raman spectrum,we find that the darkening and the trigonal shadow in the cBN crystal may be due to the presence of excess B atoms.The above-mentioned phenomenon can be determined by removing impurity capacity and growth environment of the cBN crystal.
