In the present study,volatiles from Pinus ponderosa resin extracted by conventional hydrodistillation HD and microwave hydrodistillation MHD at different powers with cryogenic CG and simple grinding SG were investigat...In the present study,volatiles from Pinus ponderosa resin extracted by conventional hydrodistillation HD and microwave hydrodistillation MHD at different powers with cryogenic CG and simple grinding SG were investigated.The gas chromatography-mass spectrometry GC-MS kinetic study showed a significant effect of heating time as a function of the various parameters,revealing variations in the qualitative and quantitative aromatic profile,often leading to the formation of oxygenated degradation compounds at the end of the extraction process,such as terpinen-4-ol,α-terpineol and trans-pinocarveol fromα-terpinene,cis-sabinene hydrate andβ-pinene respectively.Extraction time has emerged as a critical factor affecting the chemical composition of volatile oils and thus influencing their biological activities.On the other hand,the Pinus resin volatile oil exhibited a high antiinflammatory property by in vitro bovine serum albumin(BSA)denaturation assay(IC50:339.12±3.15μg/mL),in vivo test(53.11%for 400 mg/kg of body weight of rats)and in silico docking with COX-2 and MAPK2 proteins where the activity was specially enhanced by sabinene,estragole and limonene.Likewise,anti-Alzheimer activity of volatiles mainly rich in sabinene,limonene andβ-pinene was confirmed by this last technique taking.Antibacterial tests revealed interesting results compared to amoxicillin according the grinding mode studied with a high content inβ-pinene and limonene.Furthermore,this oleoresin revealed significant insecticidal activity against the fruit fly,Ceratitis capitata which can be suggested as an alternative bioinsecticide for fruits instead of chemical pesticides.展开更多
文摘In the present study,volatiles from Pinus ponderosa resin extracted by conventional hydrodistillation HD and microwave hydrodistillation MHD at different powers with cryogenic CG and simple grinding SG were investigated.The gas chromatography-mass spectrometry GC-MS kinetic study showed a significant effect of heating time as a function of the various parameters,revealing variations in the qualitative and quantitative aromatic profile,often leading to the formation of oxygenated degradation compounds at the end of the extraction process,such as terpinen-4-ol,α-terpineol and trans-pinocarveol fromα-terpinene,cis-sabinene hydrate andβ-pinene respectively.Extraction time has emerged as a critical factor affecting the chemical composition of volatile oils and thus influencing their biological activities.On the other hand,the Pinus resin volatile oil exhibited a high antiinflammatory property by in vitro bovine serum albumin(BSA)denaturation assay(IC50:339.12±3.15μg/mL),in vivo test(53.11%for 400 mg/kg of body weight of rats)and in silico docking with COX-2 and MAPK2 proteins where the activity was specially enhanced by sabinene,estragole and limonene.Likewise,anti-Alzheimer activity of volatiles mainly rich in sabinene,limonene andβ-pinene was confirmed by this last technique taking.Antibacterial tests revealed interesting results compared to amoxicillin according the grinding mode studied with a high content inβ-pinene and limonene.Furthermore,this oleoresin revealed significant insecticidal activity against the fruit fly,Ceratitis capitata which can be suggested as an alternative bioinsecticide for fruits instead of chemical pesticides.
