This review examines imaging-based nanophotonic biosensing and interferometric label-free imaging,with a particular focus on vesicle detection.It specifically compares dielectric and plasmonic metasurfaces for label-f...This review examines imaging-based nanophotonic biosensing and interferometric label-free imaging,with a particular focus on vesicle detection.It specifically compares dielectric and plasmonic metasurfaces for label-free protein and extracellular vesicle detection,highlighting their respective advantages and limitations.Key topics include:(ⅰ)refractometric sensing principles using resonant dielectric and plasmonic surfaces;(ⅱ)state-of-the-art developments in both plasmonic and dielectric nanostructured resonant surfaces;(ⅲ)a detailed comparison of resonance characteristics,including amplitude,quality factor,and evanescent field enhancement;and(ⅳ)the relationship between sensitivity,near-field enhancement,and analyte overlap in different sensing platforms.The review provides insights into the fundamental differences between plasmonic and dielectric platforms,discussing their fabrication,integration potential,and suitability for various analyte sizes.It aims to offer a unified,application-oriented perspective on the potential of these resonant surfaces for biosensing and imaging,aiming at addressing topics of interest for both photonics experts and potential users of these technologies.展开更多
Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferomet...Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferometry offers particularly high performance,but typically lacks the required operational simplicity and robustness.Here,we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability.The sensor exploits the simultaneous excitation of two orthogonally polarized modes,and detects the relative phase change caused by biomolecular binding on the sensor surface.The wide dynamic range of the sensor,which is essential for fabrication and angle tolerance,as well as versatility,is controlled by integrating multiple,tuned structures in the field of view.This approach circumvents the trade-off between sensitivity and dynamic range,typical of other phase-sensitive modalities,without increasing complexity.Our sensor enables the challenging label-free detection of procalcitonin,a small protein(13 kDa)and biomarker for infection,at the clinically relevant concentration of 1 pg mL^(−1),with a signal-to-noise ratio of 35.This result indicates the utility for an exemplary application in antibiotic guidance,and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.展开更多
In the continuous pursuit of enhancing the sensitivity of nanophotonic biosensors by leveraging phase phenomena,a recent development involved the engineering of an atomically thin Ge2Sb2Te5 layer on a silver nanofilm ...In the continuous pursuit of enhancing the sensitivity of nanophotonic biosensors by leveraging phase phenomena,a recent development involved the engineering of an atomically thin Ge2Sb2Te5 layer on a silver nanofilm to generate large Goos–Hänchen-shifts associated with phase singularities.The resulting detection limit reached~7×10^(-7)RIU.展开更多
基金supported in part by the National Institute of Health(NIH)U01CA279858,UO1CA284982,R01CA239078,RO1HL163513,R21CA267222,RO1CA264363.
文摘This review examines imaging-based nanophotonic biosensing and interferometric label-free imaging,with a particular focus on vesicle detection.It specifically compares dielectric and plasmonic metasurfaces for label-free protein and extracellular vesicle detection,highlighting their respective advantages and limitations.Key topics include:(ⅰ)refractometric sensing principles using resonant dielectric and plasmonic surfaces;(ⅱ)state-of-the-art developments in both plasmonic and dielectric nanostructured resonant surfaces;(ⅲ)a detailed comparison of resonance characteristics,including amplitude,quality factor,and evanescent field enhancement;and(ⅳ)the relationship between sensitivity,near-field enhancement,and analyte overlap in different sensing platforms.The review provides insights into the fundamental differences between plasmonic and dielectric platforms,discussing their fabrication,integration potential,and suitability for various analyte sizes.It aims to offer a unified,application-oriented perspective on the potential of these resonant surfaces for biosensing and imaging,aiming at addressing topics of interest for both photonics experts and potential users of these technologies.
基金financial support from the EPSRC of the United Kingdom(Grants EP/P02324X/1 and EP/P030017/1).Prof.Thomas F.Krauss acknowledges a Royal Society Wolfson Merit Award.Dr.Giampaolo Pitruzzello,Dr.Ben Coulson,Dr.Jose Juan-Colas,Dr.Mostafa Agour,Mr.Ben Kraus,Dr.Kezheng Li,and Dr.Stephen Thorpe contributed technical expertise and fruitful technical discussions.Dr.Ahmad Kenaan shared the PEG spacer part of the functionalization protocol.We also wish to acknowledge Thorlabs UK Ltd.for useful technical advice.
文摘Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferometry offers particularly high performance,but typically lacks the required operational simplicity and robustness.Here,we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability.The sensor exploits the simultaneous excitation of two orthogonally polarized modes,and detects the relative phase change caused by biomolecular binding on the sensor surface.The wide dynamic range of the sensor,which is essential for fabrication and angle tolerance,as well as versatility,is controlled by integrating multiple,tuned structures in the field of view.This approach circumvents the trade-off between sensitivity and dynamic range,typical of other phase-sensitive modalities,without increasing complexity.Our sensor enables the challenging label-free detection of procalcitonin,a small protein(13 kDa)and biomarker for infection,at the clinically relevant concentration of 1 pg mL^(−1),with a signal-to-noise ratio of 35.This result indicates the utility for an exemplary application in antibiotic guidance,and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.
文摘In the continuous pursuit of enhancing the sensitivity of nanophotonic biosensors by leveraging phase phenomena,a recent development involved the engineering of an atomically thin Ge2Sb2Te5 layer on a silver nanofilm to generate large Goos–Hänchen-shifts associated with phase singularities.The resulting detection limit reached~7×10^(-7)RIU.
