Basal signal transducer and activator of transcription 3(STAT3)activation is well-documented in the tumor microenvironment(TME)due to its association with cancer prognosis[1].However,its presence and clinical relevanc...Basal signal transducer and activator of transcription 3(STAT3)activation is well-documented in the tumor microenvironment(TME)due to its association with cancer prognosis[1].However,its presence and clinical relevance in the bloodstream remain unexplored.Given that STAT3-inducing cytokines,such as interleukin-6(IL-6),are often elevated in the bloodstream of various cancer patients[2,3],we aimed to investigate basal STAT3 activation in blood by developing a methodology to assess ex vivo phosphorylation of STAT3(pSTAT3ex vivo)in circulating immune cells.展开更多
基金funded by National Research Foundation of Korea(2020R1A5A2031185,2020M3A9G3080281,2022R1A2C2009385,2020M3A9G3080330,and 2022R1A6A3A01086438).
文摘Basal signal transducer and activator of transcription 3(STAT3)activation is well-documented in the tumor microenvironment(TME)due to its association with cancer prognosis[1].However,its presence and clinical relevance in the bloodstream remain unexplored.Given that STAT3-inducing cytokines,such as interleukin-6(IL-6),are often elevated in the bloodstream of various cancer patients[2,3],we aimed to investigate basal STAT3 activation in blood by developing a methodology to assess ex vivo phosphorylation of STAT3(pSTAT3ex vivo)in circulating immune cells.
