The use of constructed wetlands(CWs)is one of the best options to treat wastewater.In CWs,microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients,surfactant,and aer...The use of constructed wetlands(CWs)is one of the best options to treat wastewater.In CWs,microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients,surfactant,and aeration(NSA)in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms.In the present investigation,the influence of the addition of NSA on the persistence of the augmented bacteria,copy of(alkane hydroxylase gene)alkB gene,and its expression level in the water,soil,and plants of CWs were evaluated.The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium(Pseudomonas putida TYRI39,Acinetobacter junii TYRH47,Acinetobacter sp.CYRH17,Pseudomonas sp.CYSI27,and Pseudomonas sp.TYRH42).The mesocosms were provided with nutrients(20 mg l−1 N,2.6 mg l−1 P,and 16.4 mg l−1 K)in liquid form,surfactant Tween-20(0.2%,v/v)in liquid form,and aeration(≥7.0±1 mg l−1 DO)using aeration pump.The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water,rhizospheric soil,and plants.The maximum hydrocarbon removal(97%)was observed in the water treated by CWs having C.laevigatus,bacteria,and NSA,and it is correlated with the copy numbers of alkB and its expression level.The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.展开更多
基金the Researchers Supporting Project number(RSP2024R437)King Saud University,Riyadh,Saudi ArabiaThe authors are thankful to the Higher Education Commission(HEC)for the grant of IRSIP Fellowship to the lead author for research at Aarhus University Denmark and ALP Pakistan Agriculture Research Council(PARC)grant No.NR-086.
文摘The use of constructed wetlands(CWs)is one of the best options to treat wastewater.In CWs,microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients,surfactant,and aeration(NSA)in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms.In the present investigation,the influence of the addition of NSA on the persistence of the augmented bacteria,copy of(alkane hydroxylase gene)alkB gene,and its expression level in the water,soil,and plants of CWs were evaluated.The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium(Pseudomonas putida TYRI39,Acinetobacter junii TYRH47,Acinetobacter sp.CYRH17,Pseudomonas sp.CYSI27,and Pseudomonas sp.TYRH42).The mesocosms were provided with nutrients(20 mg l−1 N,2.6 mg l−1 P,and 16.4 mg l−1 K)in liquid form,surfactant Tween-20(0.2%,v/v)in liquid form,and aeration(≥7.0±1 mg l−1 DO)using aeration pump.The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water,rhizospheric soil,and plants.The maximum hydrocarbon removal(97%)was observed in the water treated by CWs having C.laevigatus,bacteria,and NSA,and it is correlated with the copy numbers of alkB and its expression level.The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.
