The expanding consumer interest in functional foods with amazing health benefits beyond basic nutrition has made endophytic bacteria attractive candidates for novel probiotics.Recent research has demonstrated that end...The expanding consumer interest in functional foods with amazing health benefits beyond basic nutrition has made endophytic bacteria attractive candidates for novel probiotics.Recent research has demonstrated that endophytic bacteria possess human health-promoting properties,altering gut flora and enhancing immunity.Functional food products are gaining an advantage by using these bacteria as an alternative to probiotics while providing vegan and vegetarian consumers with adequate options.Several obstacles persist,including safety evaluations,product stability during processing and effective gastrointestinal bacterial survival.The full adop-tion of endophytic bacteria depends heavily on proper regulations alongside public education.The use of endophytic bacteria helps promote green agricultural practices by reducing farmers’reliance on synthetic ad-ditives and agrochemical products.Food and agricultural applications of endophytic bacteria have the potential to reshape both eco-friendly behavior and enhance health-oriented consumer choices.The commercialization of endophytic probiotics is constrained by high production expenses,strict regulations and insufficient large-scale clinical tests.Exploiting the full potential of endophytic bacteria requires additional interdisciplinary research that combines microbiologists with food scientists and regulatory bodies.The implementation of these endo-phytic bacteria represents a breakthrough opportunity for the functional food industry to create novel probiotics that combine personalized nutrient profiles with sustainable environmental policies and global food security initiatives.This review provides details on endophytic bacteria from plants,their potential health benefits,the mechanism of action and emerging applications in functional foods.展开更多
Wheat grass,one of the members of Poaceae family,has been examined for therapeutic drugs efficiently.Wheat grass juice has become a popular beverage in various countries.Wheat grass is low in calories but high in nutr...Wheat grass,one of the members of Poaceae family,has been examined for therapeutic drugs efficiently.Wheat grass juice has become a popular beverage in various countries.Wheat grass is low in calories but high in nutrients,including antioxidants such as glutathione,vitamin C,and vitamin E.The juice is extracted from shoots of tender wheat grass and is an intensive source of minerals,vitamins,chlorophylls,antioxidants,polyphenols and active enzymes.The present investigation deals with the isolation of endophytic bacteria from wheat grass and development of probiotic formulation.A total of 25 endophytic bacteria were isolated from wheat grass and screened for pigment and hydrolytic enzyme production.The selected bacterial strains were screened for antimicrobial activity against pathogenic organisms Bacillus cereus(MTCC-430),E.coli(MTCC-1687)and Yersinia sp.(MTCC-4912).The efficient bacterial strain EU-WG-01 was identified using 16S rRNA gene sequencing as Bacillus subtilis.Bacillus subtilis EU-WG-01 was inoculated into mango juice to prepare a probiotic formulation.Bacillus subtilis EU-WG-01 at concentration of 15%was used to ferment the probiotic drink.The physiochemical(Total soluble sugars,reducing/non-reducing sugars,titratable acidity,antioxidant activity,protein content,color attributes and ascorbic acid),biomass and cell viable count were analysed.Among the physiochemical parameters a significant decrease was observed in total soluble sugar from 15.68◦brix to 16.51°◦brix,total sugars from 13.30%to 7.50%,non-reducing sugars reduced from 10.20%to 1.30%,ascorbic acid from 21.60/100 mg mL^(-1)to 14.90/100 mg mL^(-1).The antioxidant activity from 6.55%to 3.56%except for titratable acidity and reducing sugars increased significantly during 30 days storage under refrigeration.Bacillus subtilis EU-WG-01 viability was found to remain well above 2 log CFU/mL1 for 30 days.Bacillus subtilis EU-WG-01 could be used for probiotic formulation for human health.展开更多
文摘The expanding consumer interest in functional foods with amazing health benefits beyond basic nutrition has made endophytic bacteria attractive candidates for novel probiotics.Recent research has demonstrated that endophytic bacteria possess human health-promoting properties,altering gut flora and enhancing immunity.Functional food products are gaining an advantage by using these bacteria as an alternative to probiotics while providing vegan and vegetarian consumers with adequate options.Several obstacles persist,including safety evaluations,product stability during processing and effective gastrointestinal bacterial survival.The full adop-tion of endophytic bacteria depends heavily on proper regulations alongside public education.The use of endophytic bacteria helps promote green agricultural practices by reducing farmers’reliance on synthetic ad-ditives and agrochemical products.Food and agricultural applications of endophytic bacteria have the potential to reshape both eco-friendly behavior and enhance health-oriented consumer choices.The commercialization of endophytic probiotics is constrained by high production expenses,strict regulations and insufficient large-scale clinical tests.Exploiting the full potential of endophytic bacteria requires additional interdisciplinary research that combines microbiologists with food scientists and regulatory bodies.The implementation of these endo-phytic bacteria represents a breakthrough opportunity for the functional food industry to create novel probiotics that combine personalized nutrient profiles with sustainable environmental policies and global food security initiatives.This review provides details on endophytic bacteria from plants,their potential health benefits,the mechanism of action and emerging applications in functional foods.
文摘Wheat grass,one of the members of Poaceae family,has been examined for therapeutic drugs efficiently.Wheat grass juice has become a popular beverage in various countries.Wheat grass is low in calories but high in nutrients,including antioxidants such as glutathione,vitamin C,and vitamin E.The juice is extracted from shoots of tender wheat grass and is an intensive source of minerals,vitamins,chlorophylls,antioxidants,polyphenols and active enzymes.The present investigation deals with the isolation of endophytic bacteria from wheat grass and development of probiotic formulation.A total of 25 endophytic bacteria were isolated from wheat grass and screened for pigment and hydrolytic enzyme production.The selected bacterial strains were screened for antimicrobial activity against pathogenic organisms Bacillus cereus(MTCC-430),E.coli(MTCC-1687)and Yersinia sp.(MTCC-4912).The efficient bacterial strain EU-WG-01 was identified using 16S rRNA gene sequencing as Bacillus subtilis.Bacillus subtilis EU-WG-01 was inoculated into mango juice to prepare a probiotic formulation.Bacillus subtilis EU-WG-01 at concentration of 15%was used to ferment the probiotic drink.The physiochemical(Total soluble sugars,reducing/non-reducing sugars,titratable acidity,antioxidant activity,protein content,color attributes and ascorbic acid),biomass and cell viable count were analysed.Among the physiochemical parameters a significant decrease was observed in total soluble sugar from 15.68◦brix to 16.51°◦brix,total sugars from 13.30%to 7.50%,non-reducing sugars reduced from 10.20%to 1.30%,ascorbic acid from 21.60/100 mg mL^(-1)to 14.90/100 mg mL^(-1).The antioxidant activity from 6.55%to 3.56%except for titratable acidity and reducing sugars increased significantly during 30 days storage under refrigeration.Bacillus subtilis EU-WG-01 viability was found to remain well above 2 log CFU/mL1 for 30 days.Bacillus subtilis EU-WG-01 could be used for probiotic formulation for human health.
