In agriculture,fragrant and medicinal plants offer significant untapped potential for generating biomass-derived materials,particularly from wastewater by-products.In this context,the present study investigates the ch...In agriculture,fragrant and medicinal plants offer significant untapped potential for generating biomass-derived materials,particularly from wastewater by-products.In this context,the present study investigates the chemical compositions,antioxidant capacities,antimicrobial properties,acute oral toxicity,and diabetes management potential of wastewater by-products and essential oil from R.tournefortii de Noe.Utilizing advanced analytical techniques,including ATR-FTIR,GC/MS,and HPLC-DAD,were employed to conduct the analyses.ATR-FTIR analysis revealed intricate molecular compositions in both the essential oil and wastewater,confirming terpenoids,phenolic acids,and other functional groups.GC/MS and HPLC-DAD identified dominant compounds in the essential oil,notably camphor(25.49%)and 1,8-cineole(18.03%),while the wastewater contained significant levels of hydroxycinnamic acids such as caffeic acid(23.51%)and rosmarinic acid(21.15%).In terms of bioactivity,the essential oil demonstrated robust antimicrobial effects,with inhibition zones up to 17.1 mm,whereas the wastewater exhibited moderate activity,with zones up to 15.15 mm.Additionally,antioxidant assessments revealed exceptional potency of the wastewater,with IC50 values of 0.046±0.007 mg/mL for DPPH,0.082±0.013 mg/mL for ABTS,and 2.45±0.71 mg/mL for the beta-carotene/linoleate model system,surpassing the essential oil.Furthermore,both the essential oil and wastewater showed significant inhibitory effects on pancreaticα-amylase,crucial for diabetes management,with wastewater demonstrating remarkable inhibition(IC50=0.48±0.021 mg/mL).Acute oral toxicity assessment confirmed the safety of these components,alleviating concerns about potential adverse effects.In summary,these findings highlight the untapped potential and environmental significance of wastewater by-products as valuable resources,positioning R.tournefortii de Noe’s essential oil and wastewater as promising agents for sustainable healthcare and environmental sustainability.展开更多
The present work brought to light a new detailed study on the drying kinetics,also the impact of air-drying temperatures on the yield,the chemical composition,total phenol content,antioxidant,antidiabetic and antimicr...The present work brought to light a new detailed study on the drying kinetics,also the impact of air-drying temperatures on the yield,the chemical composition,total phenol content,antioxidant,antidiabetic and antimicrobials activities of Pistacia lentiscus leaf essential oil.In this study,the drying kinetics were studied over a wide temperature range of 30-70℃,to optimize drying parameters.The chemical profile revealed over 29 components of the essential oil via GC/Ms,with the major ones beingα-pinene,β-pinene,and D-limonene.Additionally,β-myrcene andβ-cymene were found in abundance.The tested biologicals activities,demonstrated notable differences in IC50 values between fresh and dried samples,especially the(IC50)values of the DPPH test which were 53.2043±0.110μg/mL and 41.2887±0.250μg/mL for the fresh and dried samples at 70℃ respectively.Reversibility was observed between the two activities,the antioxidant activity improving with higher air-drying temperatures,while antidiabetic activity was negatively impacted.The investigated fungi and bacteria appear to be sensitive to all oil tested,particularly oil extracted from the leaf dried at 40℃,with a zone of inhibition reached 32±0.3,9.8±0.3,18.02±0.1,13.5±0.3 and 16.1±0.1 mm respectively for Rhodotorula glutinis,Saccharomyces cerevisiae,Geotrichum candidum,Escherichia coli and Listeria monocytogenes.The drying process was mathematically modeled to clarify its behavior at different temperature ranges.The Midilli model effectively captured the drying curves within the temperature spectrum of 30-50℃.However,as the temperature rises to 60 and 70℃,the Two-Terms model emerged as a more fitting representation for the kinetic drying curves.The novelty of this study lies in the integration of drying kinetics,chemical composition,bioactivity and mathematical modelling to understand the impact of air-drying temperature on the Pistacia lentiscus leaf essential oil,which is essential for optimizing its therapeutic and industrial applications.展开更多
基金the International Society of Engineering Science and Technology(ISEST)UK for its financial assistancethe Researchers Supporting Project number(RSP2024R169),King Saud University,Riyadh,Saudi Arabia for the financial support.
文摘In agriculture,fragrant and medicinal plants offer significant untapped potential for generating biomass-derived materials,particularly from wastewater by-products.In this context,the present study investigates the chemical compositions,antioxidant capacities,antimicrobial properties,acute oral toxicity,and diabetes management potential of wastewater by-products and essential oil from R.tournefortii de Noe.Utilizing advanced analytical techniques,including ATR-FTIR,GC/MS,and HPLC-DAD,were employed to conduct the analyses.ATR-FTIR analysis revealed intricate molecular compositions in both the essential oil and wastewater,confirming terpenoids,phenolic acids,and other functional groups.GC/MS and HPLC-DAD identified dominant compounds in the essential oil,notably camphor(25.49%)and 1,8-cineole(18.03%),while the wastewater contained significant levels of hydroxycinnamic acids such as caffeic acid(23.51%)and rosmarinic acid(21.15%).In terms of bioactivity,the essential oil demonstrated robust antimicrobial effects,with inhibition zones up to 17.1 mm,whereas the wastewater exhibited moderate activity,with zones up to 15.15 mm.Additionally,antioxidant assessments revealed exceptional potency of the wastewater,with IC50 values of 0.046±0.007 mg/mL for DPPH,0.082±0.013 mg/mL for ABTS,and 2.45±0.71 mg/mL for the beta-carotene/linoleate model system,surpassing the essential oil.Furthermore,both the essential oil and wastewater showed significant inhibitory effects on pancreaticα-amylase,crucial for diabetes management,with wastewater demonstrating remarkable inhibition(IC50=0.48±0.021 mg/mL).Acute oral toxicity assessment confirmed the safety of these components,alleviating concerns about potential adverse effects.In summary,these findings highlight the untapped potential and environmental significance of wastewater by-products as valuable resources,positioning R.tournefortii de Noe’s essential oil and wastewater as promising agents for sustainable healthcare and environmental sustainability.
文摘The present work brought to light a new detailed study on the drying kinetics,also the impact of air-drying temperatures on the yield,the chemical composition,total phenol content,antioxidant,antidiabetic and antimicrobials activities of Pistacia lentiscus leaf essential oil.In this study,the drying kinetics were studied over a wide temperature range of 30-70℃,to optimize drying parameters.The chemical profile revealed over 29 components of the essential oil via GC/Ms,with the major ones beingα-pinene,β-pinene,and D-limonene.Additionally,β-myrcene andβ-cymene were found in abundance.The tested biologicals activities,demonstrated notable differences in IC50 values between fresh and dried samples,especially the(IC50)values of the DPPH test which were 53.2043±0.110μg/mL and 41.2887±0.250μg/mL for the fresh and dried samples at 70℃ respectively.Reversibility was observed between the two activities,the antioxidant activity improving with higher air-drying temperatures,while antidiabetic activity was negatively impacted.The investigated fungi and bacteria appear to be sensitive to all oil tested,particularly oil extracted from the leaf dried at 40℃,with a zone of inhibition reached 32±0.3,9.8±0.3,18.02±0.1,13.5±0.3 and 16.1±0.1 mm respectively for Rhodotorula glutinis,Saccharomyces cerevisiae,Geotrichum candidum,Escherichia coli and Listeria monocytogenes.The drying process was mathematically modeled to clarify its behavior at different temperature ranges.The Midilli model effectively captured the drying curves within the temperature spectrum of 30-50℃.However,as the temperature rises to 60 and 70℃,the Two-Terms model emerged as a more fitting representation for the kinetic drying curves.The novelty of this study lies in the integration of drying kinetics,chemical composition,bioactivity and mathematical modelling to understand the impact of air-drying temperature on the Pistacia lentiscus leaf essential oil,which is essential for optimizing its therapeutic and industrial applications.
