Plant extracts contain various bioactive compounds,particularly phenolic compounds(PC),which are utilized for biofilm inhibition.The Zanthoxylum armatum plant is used in various traditional medicines to treat several ...Plant extracts contain various bioactive compounds,particularly phenolic compounds(PC),which are utilized for biofilm inhibition.The Zanthoxylum armatum plant is used in various traditional medicines to treat several conditions,including stomach problems,toothache,inflammation.Nowadays,non-thermal plasma technology is gaining significant attention in the medical field and in food industry for the inactivation of pathogens.Biofilms present a significant challenge in infection treatment since they are resistant to antibiotics and bacteria within biofilms are hard to kill and making them difficult to remove and necessitating the novel strategies for their control.In this research,the combined effect of plasma NO_(x)water with phenolic compounds is found to inhibit the biofilm quorum sensing(QS)under the formation NO_(x)(NO^(-),NO_(2)^(-),and NO_(3)-)species through hydroxide oxidation via the phenolic compounds.We investigated the combined effect of plasma NO_(x)water and PC on Escherichia coli and Staphylococcus aureus biofilm quorum sensing(QS)inhibition.In the presence of NO_(x)water with PC,the E.coli and S.aureus biofilm inhibition significantly increased by 79.23%and 72.59%compared to DI water with PC and NO_(x)water.Furthermore,in the presence of NO_(x)water with PC,the AI-2 and AIP-2 QS inhibition increased than only NO_(x)water.Additionally,the viability%,exo-polysaccharides(EPS),catalase(CAT)and superoxide dismutase(SOD)enzyme activities were measured,along with intracellular ROS evaluation.Overall,the proposed combined treatment was eco-friendly for biofilm QS inhibition via NO_(x)species processed hydroxides advanced coupled oxidation.展开更多
Lung cancer continues to be the second most common cancer diagnosed and the main cause of cancer-related death globally,which requires novel and effective treatment strategies.When considering treatment options,non-sm...Lung cancer continues to be the second most common cancer diagnosed and the main cause of cancer-related death globally,which requires novel and effective treatment strategies.When considering treatment options,non-small cell lung cancer(NSCLC)remained a challenge,seeking new therapeutic strategies.High-power microwave(HPM)progressions have facilitated the advancement of new technologies as well as improvements to those al-ready in use.The impact of HPM on NSCLC has not been investigated before.In this work,we uncovered the effect of pulsed HPM on NSCLC(H460 and A549)for the first time and the most likely underlying mechanisms.Two NSCLC(H460 and A549)cells and lung normal MRC5 were exposed to HPM(15,30,45,and 60)pulses(2.1 mJ/pulse).After exposure,the effects were observed at 12,24,48,and 72 h.HPM primarily increases the level of intracellular reactive species by a strong electric field of∼27 kV/cm,which altered NSCLC viability,mitochondrial activity,and death rates.A model for the production of intracellular reactive species by HPM was also presented.NSCLC is found to be affected by HPM through DNA damage(upregulation of ATR/ATM,Chk1/Chk2,and P53)and increased expression of apoptotic markers.NAC scavenger and CPTIO-inhibitor confirm that the reactive species are mainly accountable for cellular effects.In order to ensure suitability for real-world usage,the skin depth was calculated as 30 mm.ROS played a main role in inducing cellular effects,with NO species possibly play-ing a contributing role.These findings clarify the cellular mechanisms underlying HPM-induced cell death,poten-tially advancing therapeutic approaches for treating NSCLC,and a useful first step for future investigations in this area.Moreover,this technique has the potential to serve as an adjunct to non-surgical methods in cancer therapy.展开更多
基金supported from the NRF-Korea(NRF-RS-2021-NR060112)IRTC(IITP-2024-2020-0-01846)partially supported from KwangWoon University by the“Excellent researcher project”in 2025.
文摘Plant extracts contain various bioactive compounds,particularly phenolic compounds(PC),which are utilized for biofilm inhibition.The Zanthoxylum armatum plant is used in various traditional medicines to treat several conditions,including stomach problems,toothache,inflammation.Nowadays,non-thermal plasma technology is gaining significant attention in the medical field and in food industry for the inactivation of pathogens.Biofilms present a significant challenge in infection treatment since they are resistant to antibiotics and bacteria within biofilms are hard to kill and making them difficult to remove and necessitating the novel strategies for their control.In this research,the combined effect of plasma NO_(x)water with phenolic compounds is found to inhibit the biofilm quorum sensing(QS)under the formation NO_(x)(NO^(-),NO_(2)^(-),and NO_(3)-)species through hydroxide oxidation via the phenolic compounds.We investigated the combined effect of plasma NO_(x)water and PC on Escherichia coli and Staphylococcus aureus biofilm quorum sensing(QS)inhibition.In the presence of NO_(x)water with PC,the E.coli and S.aureus biofilm inhibition significantly increased by 79.23%and 72.59%compared to DI water with PC and NO_(x)water.Furthermore,in the presence of NO_(x)water with PC,the AI-2 and AIP-2 QS inhibition increased than only NO_(x)water.Additionally,the viability%,exo-polysaccharides(EPS),catalase(CAT)and superoxide dismutase(SOD)enzyme activities were measured,along with intracellular ROS evaluation.Overall,the proposed combined treatment was eco-friendly for biofilm QS inhibition via NO_(x)species processed hydroxides advanced coupled oxidation.
基金supported by the National Research Foundation of Korea(NRF)with grants funded by the Korean government(MIST)(NRF-2022R1A2C1004257,NRF-2021R1A6A1A03038785)Kwangwoon University,Seoul,Korea,2023.
文摘Lung cancer continues to be the second most common cancer diagnosed and the main cause of cancer-related death globally,which requires novel and effective treatment strategies.When considering treatment options,non-small cell lung cancer(NSCLC)remained a challenge,seeking new therapeutic strategies.High-power microwave(HPM)progressions have facilitated the advancement of new technologies as well as improvements to those al-ready in use.The impact of HPM on NSCLC has not been investigated before.In this work,we uncovered the effect of pulsed HPM on NSCLC(H460 and A549)for the first time and the most likely underlying mechanisms.Two NSCLC(H460 and A549)cells and lung normal MRC5 were exposed to HPM(15,30,45,and 60)pulses(2.1 mJ/pulse).After exposure,the effects were observed at 12,24,48,and 72 h.HPM primarily increases the level of intracellular reactive species by a strong electric field of∼27 kV/cm,which altered NSCLC viability,mitochondrial activity,and death rates.A model for the production of intracellular reactive species by HPM was also presented.NSCLC is found to be affected by HPM through DNA damage(upregulation of ATR/ATM,Chk1/Chk2,and P53)and increased expression of apoptotic markers.NAC scavenger and CPTIO-inhibitor confirm that the reactive species are mainly accountable for cellular effects.In order to ensure suitability for real-world usage,the skin depth was calculated as 30 mm.ROS played a main role in inducing cellular effects,with NO species possibly play-ing a contributing role.These findings clarify the cellular mechanisms underlying HPM-induced cell death,poten-tially advancing therapeutic approaches for treating NSCLC,and a useful first step for future investigations in this area.Moreover,this technique has the potential to serve as an adjunct to non-surgical methods in cancer therapy.
