This 60-day culture trial was conducted to investigate the effects of flavone on the growth performance and intestinal microbiota of Litopenaeus vannamei at high temperatures utilizing transcriptomics and metabolomics...This 60-day culture trial was conducted to investigate the effects of flavone on the growth performance and intestinal microbiota of Litopenaeus vannamei at high temperatures utilizing transcriptomics and metabolomics analysis.A total of 240 healthy shrimp(Average weight 2.62±0.06 g)were administered diets containing four different levels of flavone:0 g/kg(control),5 g/kg(FLV1),10 g/kg(FLV2)and 15 g/kg(FLV3).Intestine and hepatopancreas tissues from the control and treatment groups were analyzed for changes in microbiota,transcriptome and metabolome.Shrimps fed with an experimental diet exhibited a significant increase in growth performance indices compared to the control group.The most efficient feed conversion ratio(FCR)was recorded in the FLV2 group compared to other groups,proving the efficacy of flavone as an additive.Specifically,the thermal tolerance test revealed an increase in the survival rates of shrimps fed an enriched diet compared to the control group.The result also showed that the survival rate decreased as the temperature reached 40℃.The intestinal microbiome composition showed a significant increase in the abundance of Firmicutes and Actinobacteria.The transcriptomic analysis highlighted the up-regulation of Glutathione S-transferase and pancreatic lipase-related protein,while the macrophage mannose receptor and Maltase-glucoamylase expression was down-regulated.Metabolomic analysis identified 4393 significantly differing metabolites,of which 966 were upregulated and 1279 downregulated.The integrated transcriptomic and metabolomic analysis identified upregulated genes(gk,ar,dgk,gpat3,agpat-1,gplat1,pnpla2,and plrp2)in the glycerolipid metabolism pathway responsible for lipid metabolism.Dietary flavone played a significant role in growth performance,intestinal health and physiology of Litopenaeus vannamei.展开更多
基金supported by Korea Institute of Marine Science&Technology Promotion(KIMST)funded by the Ministry of Oceans and Fisheries(RS-2022-KS221673,Big data-based aquaculture productivity improvement technology)Zhou Finance and Agriculture[2024]No.15 Research and Demonstration on High-Efficiency Facility-Based Cultivation of Shrimps+3 种基金Zhejiang Province’s Science and Technology Special Envoy Team’Aquatic Products Science and Technology Service Team’Project(210380010020)Municipal public welfare technology projects(2023C31045)Zhoushan municipal special fund for modern fishery development Zhou Cainong[2022]No.15the project of science and technology of Zhoushan(2021C31003).
文摘This 60-day culture trial was conducted to investigate the effects of flavone on the growth performance and intestinal microbiota of Litopenaeus vannamei at high temperatures utilizing transcriptomics and metabolomics analysis.A total of 240 healthy shrimp(Average weight 2.62±0.06 g)were administered diets containing four different levels of flavone:0 g/kg(control),5 g/kg(FLV1),10 g/kg(FLV2)and 15 g/kg(FLV3).Intestine and hepatopancreas tissues from the control and treatment groups were analyzed for changes in microbiota,transcriptome and metabolome.Shrimps fed with an experimental diet exhibited a significant increase in growth performance indices compared to the control group.The most efficient feed conversion ratio(FCR)was recorded in the FLV2 group compared to other groups,proving the efficacy of flavone as an additive.Specifically,the thermal tolerance test revealed an increase in the survival rates of shrimps fed an enriched diet compared to the control group.The result also showed that the survival rate decreased as the temperature reached 40℃.The intestinal microbiome composition showed a significant increase in the abundance of Firmicutes and Actinobacteria.The transcriptomic analysis highlighted the up-regulation of Glutathione S-transferase and pancreatic lipase-related protein,while the macrophage mannose receptor and Maltase-glucoamylase expression was down-regulated.Metabolomic analysis identified 4393 significantly differing metabolites,of which 966 were upregulated and 1279 downregulated.The integrated transcriptomic and metabolomic analysis identified upregulated genes(gk,ar,dgk,gpat3,agpat-1,gplat1,pnpla2,and plrp2)in the glycerolipid metabolism pathway responsible for lipid metabolism.Dietary flavone played a significant role in growth performance,intestinal health and physiology of Litopenaeus vannamei.
