The bioactivity of carotenoids and tocols is limited by their instability,hydrophobicity,and low bioavailability.Hence,the study aimed to design microspheres from sea buckthorn juice by ionotropic gelation to increase...The bioactivity of carotenoids and tocols is limited by their instability,hydrophobicity,and low bioavailability.Hence,the study aimed to design microspheres from sea buckthorn juice by ionotropic gelation to increase stability,ensure high content of carotenes,xanthophylls,tocopherols and tocotrienols and their antidiabetic activity.The effects of biopolymers,cross-linking and storage on the carotenoid and tocol profile of microspheres(identified and quantified by LC-PDA-QTOF-ESI-MS and UPLC-FL methods)and their in vitro α-amylase and α-glucosidase inhibitory activity were investigated.Microspheres containing sodium alginate/κ-carrageenan(4:1)had significantly high content of carotenoids and tocols,and antihyperglycemic activity(p<0.05).The two-step cross-linking(1-calcium chloride,2-chitosan)provided a 17%higher carotenoid content.Cross-linking with CaCl_(2)promoted high content of tocopherols but stability protection was provided by sodium alginate and CaCl_(2)/chitosan(9:1)cross-linking(1.7-fold decrease in instability).The encapsulation using newly designed combinations of biopolymers and cross-linking could be an effective strategy to increase the stability and bioactivities of microspheres as nutraceuticals and smart food.展开更多
In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyz...In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyzed for physicochemical and biological properties.Fermentation and carrier type affected the powders’quality to a greater extent than drying techniques.Higher betalains content was noted for non-fermented juice powders,while ferulic and syringic acids’derivatives for their fermented-juice-based counterparts.Oligofructose induced hydroxymethyl-L-furfural formation,but also together with inulin resulted in products with the strongest antioxidant capacity.Nutriose®had the greatest in vitro antiproliferative activity towards human leukemia cell lines,unlike oligofructose which was shown to stimulate their growth.Overall,fermentation that led to beetroot matrix modification and carrier type affected powders’quality toward their improved potential functional properties.展开更多
基金funded in part by the National Science Centre,Poland(NCN)under the project Preludium 20,grant number UMO-2021/41/N/NZ9/02790The analysis of tocopherols and tocotrienols was performed in the framework of the Ph.D.research program Innowacyjny Doktorat,grant number N070/0016/20,Wrocław University of Environmental and Life Sciences,Poland+1 种基金The APC is financed by Wrocław University of Environmental and Life Sciences,Polandsupported by the Foundation for Polish Science(FNP).The publication is the result of the activity of the research group Plants4FOOD.
文摘The bioactivity of carotenoids and tocols is limited by their instability,hydrophobicity,and low bioavailability.Hence,the study aimed to design microspheres from sea buckthorn juice by ionotropic gelation to increase stability,ensure high content of carotenes,xanthophylls,tocopherols and tocotrienols and their antidiabetic activity.The effects of biopolymers,cross-linking and storage on the carotenoid and tocol profile of microspheres(identified and quantified by LC-PDA-QTOF-ESI-MS and UPLC-FL methods)and their in vitro α-amylase and α-glucosidase inhibitory activity were investigated.Microspheres containing sodium alginate/κ-carrageenan(4:1)had significantly high content of carotenoids and tocols,and antihyperglycemic activity(p<0.05).The two-step cross-linking(1-calcium chloride,2-chitosan)provided a 17%higher carotenoid content.Cross-linking with CaCl_(2)promoted high content of tocopherols but stability protection was provided by sodium alginate and CaCl_(2)/chitosan(9:1)cross-linking(1.7-fold decrease in instability).The encapsulation using newly designed combinations of biopolymers and cross-linking could be an effective strategy to increase the stability and bioactivities of microspheres as nutraceuticals and smart food.
基金supported by“UPWR 2.0:international and interdisciplinary programme of development of Wrocław University of Environmental and Life Sciences”co-financed by the European Social Fund under the Operational Program Knowledge Education Development,under contract No.POWR.03.05.00-00-Z062/18 of June 4,2019.
文摘In the present study freeze-and spray-dried powders from non-fermented and fermented beetroot juice with the addition of prebiotic carriers(inulin,oligofructose,Nutriose®)and maltodextrin were obtained and analyzed for physicochemical and biological properties.Fermentation and carrier type affected the powders’quality to a greater extent than drying techniques.Higher betalains content was noted for non-fermented juice powders,while ferulic and syringic acids’derivatives for their fermented-juice-based counterparts.Oligofructose induced hydroxymethyl-L-furfural formation,but also together with inulin resulted in products with the strongest antioxidant capacity.Nutriose®had the greatest in vitro antiproliferative activity towards human leukemia cell lines,unlike oligofructose which was shown to stimulate their growth.Overall,fermentation that led to beetroot matrix modification and carrier type affected powders’quality toward their improved potential functional properties.
