Biomaterials are widely used as orthopaedic implants and bone graft substitutes.We aimed to develop a rapid osteogenic assessment method using a murine tibial periosteal ossification model to evaluate the bone forma-t...Biomaterials are widely used as orthopaedic implants and bone graft substitutes.We aimed to develop a rapid osteogenic assessment method using a murine tibial periosteal ossification model to evaluate the bone forma-tion/remodelling potential of a biomaterial within 2-4 weeks.A novel hydroxyapatite/aragonite(HAA)biomaterial was implanted into C57BL/6 mice juxtaskeletally between the tibia and tibialis anterior muscle.Rapid intramembranous bone formation was observed at 14 days,with 4-to 8-fold increases in bone thickness and callus volume in comparison with sham-operated animals(p<0.0001),followed by bone remodelling and a new layer of cortical bone formation by 28 days after implantation.The addition of zoledronate,a clinically-utilised bisphosphonate,to HAA,promoted significantly more new bone formation than HAA alone over 28 days(p<0.01).The osteogenic potential of HAA was further confirmed by implanting into a 3.5 mm diameter femoral cancellous bone defect in rats and a 5 mm diameter femoral cortical bone defect in minipigs.To un-derstand the biodegradation and the cellular activity at the cell/biomaterial interfaces,non-decalcified speci-mens were resin embedded and sections subjected to combined scanning electron microscopy(SEM)/electron backscatter diffraction(EBSD)/energy dispersive X-ray spectrometry(EDS)analysis.We conclude that murine tibial periosteal ossification is a novel method for rapid assessment of the interaction of bioactive materials with osteogenic tissues.This study also highlights that combining calcium carbonate with hydroxyapatite enhances biodegradation and osteogenesis.展开更多
基金supported by Royal Society International Exchanges Grant 2021(IES\R1\211128)Qu´ebec-Wales Collaboration Grant 2021 and Wales Innovation Network(WIN)Small Grants Fund(SG6-RIR1058-107).
文摘Biomaterials are widely used as orthopaedic implants and bone graft substitutes.We aimed to develop a rapid osteogenic assessment method using a murine tibial periosteal ossification model to evaluate the bone forma-tion/remodelling potential of a biomaterial within 2-4 weeks.A novel hydroxyapatite/aragonite(HAA)biomaterial was implanted into C57BL/6 mice juxtaskeletally between the tibia and tibialis anterior muscle.Rapid intramembranous bone formation was observed at 14 days,with 4-to 8-fold increases in bone thickness and callus volume in comparison with sham-operated animals(p<0.0001),followed by bone remodelling and a new layer of cortical bone formation by 28 days after implantation.The addition of zoledronate,a clinically-utilised bisphosphonate,to HAA,promoted significantly more new bone formation than HAA alone over 28 days(p<0.01).The osteogenic potential of HAA was further confirmed by implanting into a 3.5 mm diameter femoral cancellous bone defect in rats and a 5 mm diameter femoral cortical bone defect in minipigs.To un-derstand the biodegradation and the cellular activity at the cell/biomaterial interfaces,non-decalcified speci-mens were resin embedded and sections subjected to combined scanning electron microscopy(SEM)/electron backscatter diffraction(EBSD)/energy dispersive X-ray spectrometry(EDS)analysis.We conclude that murine tibial periosteal ossification is a novel method for rapid assessment of the interaction of bioactive materials with osteogenic tissues.This study also highlights that combining calcium carbonate with hydroxyapatite enhances biodegradation and osteogenesis.
