The effect of fermentation by Saccharomyces cerevisiae on biological properties of cinnamon(Cinnamomum cassia)was investigated.The study demonstrated that the extract of S.cerevisiae-fermented cinnamon(S.C.FC)has anti...The effect of fermentation by Saccharomyces cerevisiae on biological properties of cinnamon(Cinnamomum cassia)was investigated.The study demonstrated that the extract of S.cerevisiae-fermented cinnamon(S.C.FC)has antioxidants higher than non-fermented one.The optimum results for antioxidant yield were noted with 10^(7)CFU S.cerevisiae/10 g cinnamon and 70 mL of dH_(2)O at pH 6 and incubated for 3 d at 35℃.Under optimum conditions,ABTS,DPPH,and H_(2)O_(2)radical-scavenging activity increased by 43.8,61.5,and 71.9%,respectively.Additionally,the total phenols and flavonoids in S.C.FC were increased by 81.3 and 415%compared by non-fermented one.The fermented cinnamon had antimicrobial activity against L.monocytogenes,S.aureus,E.coli,S.typhi,and C.albicans.Also,the antiinflammatory properties were increased from 89 to 92%after fermentation.The lyophilized extract of S.C.FC showed positive effect against Huh7 cancer cells which decreased by 31%at the concentration of 700μg/mL.According to HPLC analysis,p-hydroxybenzoic acid,gentisic acid,catechin,chlorogenic acid,caffeic acid,and syringic acid were increased by 116,33.2,59.6,50.6,1.6,and 16.9%,respectively.Our findings suggest the applicability of cinnamon fermentation using S.cerevisiae as a useful tool for processing functional foods to increase their antioxidant and antiinflammatory content.展开更多
Polyester/cotton(PET/C)blended fabric wastes are produced daily in huge amounts,which constitutes an economic loss and an environmental threat if it is not reused appropriately.Modern textile waste recycling technolog...Polyester/cotton(PET/C)blended fabric wastes are produced daily in huge amounts,which constitutes an economic loss and an environmental threat if it is not reused appropriately.Modern textile waste recycling technologies put much effort into developing fabric materials with unique properties,such as bioactivity or new optical goods based on modern technologies,especially nano-biotechnology.In this study,zinc oxide nanoparticles(ZnO-NPs)were biosynthesized using the aqueous extract of Dunaliella sp.and immobilized on PET/C waste fabrics after enzymatically activated with cellulases.The produced Dunaliella-ZnO-NPs(10–20 nm with a spherical shape)were characterized by High-resolution transmission electron microscopy(HRTEM),Fourier-transform infrared spectroscopy(FTIR),X-Ray diffraction analysis(XRD),and Scanning electron microscopy-energy dispersive X-ray analyzer(SEM-EDAX),and some functional groups,such as CH,CO,NH,and CN(due to the presence of carboxyl,proteins and hydroxyl groups),were detected,revealing the biosynthesis of ZnO-NPs.The analysis showed that the resulting ZnO-NPS had potent antimicrobial effects,Ultraviolet(UV)protection capabilities,and no cytotoxic effects on the normal human fibroblast cell line(BJ1).On the other hand,enzymatic treatments of PET/C fabric waste with cellulases enhanced the immobilization of biosynthetic nanoparticles on their surface.Modified PET/C fabrics loaded with Dunaliella-ZnO-NPs showed antibacterial and UV protection capabilities making them an eco-friendly and cost-effective candidate for numerous applications.These applications can include the manufacture of active packaging devices,wastewater treatment units,and many other environmental applications.展开更多
基金funding provided by The Science,Technology&Innovation Funding Authority(STDF)in cooperation with The Egyptian Knowledge Bank(EKB)。
文摘The effect of fermentation by Saccharomyces cerevisiae on biological properties of cinnamon(Cinnamomum cassia)was investigated.The study demonstrated that the extract of S.cerevisiae-fermented cinnamon(S.C.FC)has antioxidants higher than non-fermented one.The optimum results for antioxidant yield were noted with 10^(7)CFU S.cerevisiae/10 g cinnamon and 70 mL of dH_(2)O at pH 6 and incubated for 3 d at 35℃.Under optimum conditions,ABTS,DPPH,and H_(2)O_(2)radical-scavenging activity increased by 43.8,61.5,and 71.9%,respectively.Additionally,the total phenols and flavonoids in S.C.FC were increased by 81.3 and 415%compared by non-fermented one.The fermented cinnamon had antimicrobial activity against L.monocytogenes,S.aureus,E.coli,S.typhi,and C.albicans.Also,the antiinflammatory properties were increased from 89 to 92%after fermentation.The lyophilized extract of S.C.FC showed positive effect against Huh7 cancer cells which decreased by 31%at the concentration of 700μg/mL.According to HPLC analysis,p-hydroxybenzoic acid,gentisic acid,catechin,chlorogenic acid,caffeic acid,and syringic acid were increased by 116,33.2,59.6,50.6,1.6,and 16.9%,respectively.Our findings suggest the applicability of cinnamon fermentation using S.cerevisiae as a useful tool for processing functional foods to increase their antioxidant and antiinflammatory content.
基金supported by the program of the science,technology and innovation funding authority(STDF),Egypt under Grant No.43447.
文摘Polyester/cotton(PET/C)blended fabric wastes are produced daily in huge amounts,which constitutes an economic loss and an environmental threat if it is not reused appropriately.Modern textile waste recycling technologies put much effort into developing fabric materials with unique properties,such as bioactivity or new optical goods based on modern technologies,especially nano-biotechnology.In this study,zinc oxide nanoparticles(ZnO-NPs)were biosynthesized using the aqueous extract of Dunaliella sp.and immobilized on PET/C waste fabrics after enzymatically activated with cellulases.The produced Dunaliella-ZnO-NPs(10–20 nm with a spherical shape)were characterized by High-resolution transmission electron microscopy(HRTEM),Fourier-transform infrared spectroscopy(FTIR),X-Ray diffraction analysis(XRD),and Scanning electron microscopy-energy dispersive X-ray analyzer(SEM-EDAX),and some functional groups,such as CH,CO,NH,and CN(due to the presence of carboxyl,proteins and hydroxyl groups),were detected,revealing the biosynthesis of ZnO-NPs.The analysis showed that the resulting ZnO-NPS had potent antimicrobial effects,Ultraviolet(UV)protection capabilities,and no cytotoxic effects on the normal human fibroblast cell line(BJ1).On the other hand,enzymatic treatments of PET/C fabric waste with cellulases enhanced the immobilization of biosynthetic nanoparticles on their surface.Modified PET/C fabrics loaded with Dunaliella-ZnO-NPs showed antibacterial and UV protection capabilities making them an eco-friendly and cost-effective candidate for numerous applications.These applications can include the manufacture of active packaging devices,wastewater treatment units,and many other environmental applications.
