Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify se...Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.展开更多
RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pe...RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata.展开更多
Inherited bacterial symbionts are very common in arthropods, but infection frequency can vary widely among populations. Experiments and interpopulation comparisons suggest that host genetic background might be importa...Inherited bacterial symbionts are very common in arthropods, but infection frequency can vary widely among populations. Experiments and interpopulation comparisons suggest that host genetic background might be important in explaining this variation. Our extensive field investigation showed that the infection pattern of the facultative symbiont Cardinium was heterogeneous across geographical populations of the invasive whitefly Bemisia tabaci Mediterranean (MED) in China, with genetic nuclear differences evident in 2 of the populations: 1 with a low infection rate (SD line) and 1 with a high infection rate (HaN line). However, whether the heterogeneous frequency of Cardinium is associated with the host genetic background remains poorly understood. Here, we compared the fitness of the Cardinium-infected and uninfected sublines with similar nuclear genetic backgrounds from SD and HaN lines, respectively, and further determine whether host extranuclear or nuclear genotype influenced the Cardinium–host phenotype by performing 2 new introgression series of 6 generations between SD and HaN lines (i.e., Cardinium-infected females of SD were backcrossed with uninfected males of HaN, and vice versa). The results showed that Cardinium provides marginal fitness benefits in the SD line, whereas Cardinium provides strong fitness benefits in the HaN line. Further, both Cardinium and the Cardinium–host nuclear interaction influence the fecundity and pre-adult survival rate of B. tabaci, whereas the extranuclear genotype does not. In conclusion, our results provide evidence that Cardinium-mediated fitness effects were closely associated with the host genetic background, which provides a fundamental basis for understanding the mechanism underlying the heterogeneous distribution of Cardinium in B. tabaci MED populations across China.展开更多
基金the National Key R and DProgram of China(Grant No.2017YFD0200900)the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017).
文摘Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.
基金supported by the National Key R&D Program of China(2017YFD0200900)National Natural Science Foundation of China(31972269)GDUPS(2017).
文摘RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata.
基金supported by the National Key R&D Program of China(2022YFD1401200)Taishan Scholar Foundation of Shandong Province of China(tstp20221135)the National Nature Science Foun-dation of China(31872030 and 31572064).
文摘Inherited bacterial symbionts are very common in arthropods, but infection frequency can vary widely among populations. Experiments and interpopulation comparisons suggest that host genetic background might be important in explaining this variation. Our extensive field investigation showed that the infection pattern of the facultative symbiont Cardinium was heterogeneous across geographical populations of the invasive whitefly Bemisia tabaci Mediterranean (MED) in China, with genetic nuclear differences evident in 2 of the populations: 1 with a low infection rate (SD line) and 1 with a high infection rate (HaN line). However, whether the heterogeneous frequency of Cardinium is associated with the host genetic background remains poorly understood. Here, we compared the fitness of the Cardinium-infected and uninfected sublines with similar nuclear genetic backgrounds from SD and HaN lines, respectively, and further determine whether host extranuclear or nuclear genotype influenced the Cardinium–host phenotype by performing 2 new introgression series of 6 generations between SD and HaN lines (i.e., Cardinium-infected females of SD were backcrossed with uninfected males of HaN, and vice versa). The results showed that Cardinium provides marginal fitness benefits in the SD line, whereas Cardinium provides strong fitness benefits in the HaN line. Further, both Cardinium and the Cardinium–host nuclear interaction influence the fecundity and pre-adult survival rate of B. tabaci, whereas the extranuclear genotype does not. In conclusion, our results provide evidence that Cardinium-mediated fitness effects were closely associated with the host genetic background, which provides a fundamental basis for understanding the mechanism underlying the heterogeneous distribution of Cardinium in B. tabaci MED populations across China.
