Areca nut is the basic ingredient of betel quid,which is chewed by hundreds of millions of people in South-Eastern Asia.Chewing of areca nut has been associated with oral cancers potentially due to its specific alkalo...Areca nut is the basic ingredient of betel quid,which is chewed by hundreds of millions of people in South-Eastern Asia.Chewing of areca nut has been associated with oral cancers potentially due to its specific alkaloids,among which arecoline constitutes about 90%of total fraction.Being the world’s fourth most commonly used psychoactive substance,arecoline evokes stimulation,addiction,and other direct neurological effects,while its misuse correlates to neurotoxic effects.However,what might underlie its neurotoxic mechanisms has been poorly documented.The brain is encoded by a complex network of neuronal and glial cell types,and neurotoxicity of hazardous compounds present transcriptional heterogeneity.Recently,the eusocial bumblebee has been used as a model for studying brain effects,with sophisticated cognitive capability and precisely measured brain architecture.Here,we decipher cell-type-specific mental risks to arecoline using bumblebees.Arecoline induced locomotor hyperactivity and cognitive impairment.Single nucleus RNA sequencing(snRNA-seq)unearthed arecoline-induced cell-specific responses,primarily targeted on Kenyon cells(KC).Moreover,high-dose arecoline induced distinctive cell responses among KC subtypes,particularly class I large Kenyon cell(lKC),leading to DNA damage,excitatory/inhibitory(E/I)imbalance,and calcium dyshomeostasis,which potentially resulted in cognitive impairment.Given arecoline’s popularity and growing exposure risks to humans,neurological health risks of areca nut warrant serious consideration.展开更多
Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropria...Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropriate handling of CFR not only waste valuable bioresources,but may also lead to the cyclosporin A and associated resistance genes into the natural environment,posing a significant threat to ecological system and human health.Land application was an effective way to resource recovery of CFR after aerobic composting(CAC).This study investigated the impact of CAC on soil fertility and environmental safety.The results indicated that CAC could improve soil nutrient contents and enhance enzyme activities.CAC altered the diversity and community composition of soil bacteria,resulting in an increase in the abundance of relevant bacteria beneficial for organic matter decomposition and cyclosporin A degradation.The introduced cyclosporin A(71.69μg/kg)completely degraded within 20 days due to soil biodegradation.The significantly increased abundance of intIl,mdr3,pgp,TSR and pmra in the soil cultivation early stage were restored to the soil background level within 90 days,indicating a reduced risk of antimicrobial resistance.The results demonstrated that reasonable land application of CAC could improve soil fertility without antimicrobial resistance risk,which is helpful for evaluating the resource utilization value and environmental risks of antibiotic fermentation residue after aerobic composting.展开更多
Due to the complex natures of dietary food components,it is difficult to elucidate how the compounds affect host health.Dietary food often selectively presents its mechanism of action on different cell types,and parti...Due to the complex natures of dietary food components,it is difficult to elucidate how the compounds affect host health.Dietary food often selectively presents its mechanism of action on different cell types,and participates in the modulation of targeted cells and their microenvironments within organs.However,the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased influences.Single-cell RNA sequencing(sc RNA-seq)has emerged as an indispensable methodology to decompose tissues into different cell types for the demonstration of transcriptional profiles of individual cells.Sc RNA-seq applications has been summarized on three typical organs(brain,liver,kidney),and two representative immune-and tumor related health problems.The everincreasing role of sc RNA-seq in dietary food research with further improvement can provide sub-cellular information and the coupling between other cellular modalities.In this review,we propose utilizing sc RNAseq to more effectively capture the subtle and complex effects of food chemicals,and how they may lead to health problems at single-cell resolution.This novel technique will be valuable to elucidate the underlying mechanism of both the health benefits of food nutrients and the detrimental consequences food toxicants at the cellular level.展开更多
Tri(2-chloropropyl)phosphate(TCPP)was an emerging contaminant of global concern because of its frequent occurrence,potential toxic effects,and persistence in the environment.Microbial degradation might be an efficient...Tri(2-chloropropyl)phosphate(TCPP)was an emerging contaminant of global concern because of its frequent occurrence,potential toxic effects,and persistence in the environment.Microbial degradation might be an efficient and safe removal method,but limited information was available.In this study,Providencia rettgeri was isolated from contaminated sediment and showed it could use TCPP as unique phosphorus source to promote growth,and decompose 34.7%of TCPP(1 mg/L)within 5 days.The microbial inoculation and the initial concentration of TCPP could affect the biodegradation efficient.Further study results indicated that TCPP decomposition by Providencia rettgeri was mainly via phosphoester bond hydrolysis,evidenced by the production of bis(2-chloropropyl)phosphate(C_(6)H_(13)Cl_(2)PO_(4))and mono-chloropropyl phosphate(C_(3)H_(8)ClPO_(4)).Both intracellular and extracellular enzymes could degrade TCPP,but intracellular degradation was dominant in the later reaction stage,and the presence of Cu^(2+) ions had a promoting effect.These findings developed novel insights into the potential mechanism of TCPP microbial degradation.展开更多
Metal-organic frameworks(MOFs)functionalized with open metal sites(OMSs)have received widespread attention in various applications due to their fascinating electronic properties and unique interactions with vip mole...Metal-organic frameworks(MOFs)functionalized with open metal sites(OMSs)have received widespread attention in various applications due to their fascinating electronic properties and unique interactions with vip molecules.However,rational tailoring of the coordination environment of metal nodes dur-ing the synthesis of MOFs remains a great challenge due to their tendency of saturated coordination with linkers.Herein,we reported the construction of three new MOFs featuring unsaturated Cu(Ⅱ)sites,namely[Cu(HCOO)(pzta)]_(n)(HL-1),{[Cu(PTA)0.5(pzta)(H_(2)O)]·2H_(2)O}_(n)(HL-2)and[Cu(NA)0.5(pzta)]_(n)(HL-3)(Hpzta=3-pyrazinyl-1,2,4-triazole;PTA=terephthalic acid;NA=1,4-naphthalene dicarboxylic acid),based on the mixed-linker strategy via specific selection of versatile Hpzta ligand and carboxylate ligands.Re-markably,the obtained MOFs exhibited excellent activity and good recyclability for the catalytic reduction of nitroaromatics under mild conditions(25℃and 1 atm).In particular,the complete conversion of 4-nitrophenol(4-NP)took only 30 s on HL-2,reaching a record-high TOF value compared with previously reported metal catalysts.The combined experimental and theoretical studies on HL-2 revealed that the open Cu site with positive-charged nature could improve the adsorption and subsequent electron trans-port between the substrates,and was responsible for the outstanding performance.This work shined lights on the further enhancement of performance for MOFs through rational OMSs construction.展开更多
Policymakers and managers have increasingly adopted green bonds as a direct financing tool to address environmental degradation and climate change in emerging economies;however,the increasing green washing sentiments ...Policymakers and managers have increasingly adopted green bonds as a direct financing tool to address environmental degradation and climate change in emerging economies;however,the increasing green washing sentiments in the green bond market raise questions on whether green bonds can nudge polluting businesses to achieve green transformation.Therefore,this study joins the controversial debate by investigating the impact of green bond issuance on corporate environmental responsibilities and the potential impact mechanisms and economic consequences.Using the data of Chinese listed enterprises from 2011 to 2020 and the staggered issuance of green bonds as plausibly exogenous shocks,we determine that the enterprises in the experimental group that issued green bonds increased their environmental performance compared to their counterparts.Furthermore,this positive link is maintains after a series of robustness tests.Moreover,we identify that green bond issuance plausibly enhances environmental responsibility engagements through two governance channels,namely,internal management and external supervision.This beneficial effect appears more pronounced for subsamples of firms in low-polluting industries,without environmental subsidies and with higher managerial abilities.Furthermore,economic consequences indicate that the issuance of green bonds primarily motivates speculative shareholder benefits,as evidenced by short-term increases in stock yields but with limited impact on the short-run financial performance.Overall,these findings offer new evidence supporting that green financing tools could play a helpful hand toward environmental sustainability.展开更多
Objective: To explore the correlation between muscle strength, muscle mass and bone mineral density (BMD) in Zhuang female population, body composition analysis and grip strength, and to analyze the possible influenci...Objective: To explore the correlation between muscle strength, muscle mass and bone mineral density (BMD) in Zhuang female population, body composition analysis and grip strength, and to analyze the possible influencing factors of BMD. Methods: 182 postmenopausal women were selected from Guangxi Province of China. Broadband ultrasound attenuation (BUA) was used to evaluate BMD. Grip dynamometer to assess muscle strength. Height, weight and muscle mass of each part were measured by body composition measuring instrument. Body mass index (BMI), skeletal muscle mass index (SMI) and limb skeletal muscle mass (SM) were calculated according to the measurement results. Results: BUA, grip strength and SMI in postmenopausal women of Zhuang nationality showed a decreasing trend with age (p p p r = 0.305, p Conclusion: With the increase of age, the decline rate of muscle strength of postmenopausal Zhuang women in Guangxi is slower than that of BMD and muscle mass. SM can better reflect the BMD level of the body than SMI, and the LSM is the main influencing factor of BMD.展开更多
Combinations of graphene(Gr)and carbon black(C)were employed as binary carbon supports to fabricate Pd‐based electrocatalysts via one‐pot co‐reduction with Pd2+.The electrocatalytic performance of the resulting Pd...Combinations of graphene(Gr)and carbon black(C)were employed as binary carbon supports to fabricate Pd‐based electrocatalysts via one‐pot co‐reduction with Pd2+.The electrocatalytic performance of the resulting Pd/Gr‐C catalysts during the electrooxidation of formic acid was assessed.A Pd/Gr0.3C0.7(Gr oxide:C=3:7,based on the precursor mass ratio)electrocatalyst exhibited better catalytic performance than both Pd/C and Pd/Gr catalysts.The current density generated by the Pd/Gr0.3C0.7catalyst was as high as102.14mA mgPd?1,a value that is approximately3times that obtained from the Pd/C(34.40mA mgPd?1)and2.6times that of the Pd/Gr material(38.50mA mgPd?1).The anodic peak potential of the Pd/Gr0.3C0.7was120mV more negative than that of the Pd/C and70mV more negative than that of the Pd/Gr.Scanning electron microscopy images indicated that the spherical C particles accumulated on the wrinkled graphene surfaces to form C cluster/Gr hybrids having three‐dimensional nanostructures.X‐ray photoelectron spectroscopy data confirmed the interaction between the Pd metal and the binary Gr‐C support.The Pd/Gr0.3C0.7also exhibited high stability,and so is a promising candidate for the fabrication of anodes for direct formic acid fuel cells.This work demonstrates a simple and cost‐effective method for improving the performance of Pd‐based electrocatalysts,which should have potential industrial applications.展开更多
Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influe...Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast(OB, bone forming cells) responses was investigated. Nanocrystalline diamond(NCD) and submicron crystalline diamond(SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition(MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay(ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates(each containing 30–50 cells) on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.展开更多
Abstract Spinal cord injury （SCI） remains an unsolved human health challenge. To alleviate the impairments of SCI, we studied the therapeutic effect of nimodipine （an L-type Ca^2＋ channel antagonist） on functiona...Abstract Spinal cord injury （SCI） remains an unsolved human health challenge. To alleviate the impairments of SCI, we studied the therapeutic effect of nimodipine （an L-type Ca^2＋ channel antagonist） on functional recovery from SCI using Nystrom＇s method in a mouse model. Eighty-four mice were divided into three groups： control group in which only vertebral plates were cut off without causing any spinal injuries; SCI; and SCI with nimodipine treatment. We assessed the histopathology, apoptosis detection, cell cycle, mitochondrial transmembrane potential, bcl-2/bax and caspase-3 levels of tissue 8 h, 1 d, 3 d and 4 d after trauma to evaluate rehabilitation. Behavioral performances were also assessed before and after nimodipine treatment. Results from inclined plane tests, motor score assessment and histological observations indicated that mice in the nimodipine-treated group rehabilitated better than those in the SCI group. The ratio of apoptosis, caspase-3 and bax expression in the nimodip- ine-treated group were significantly lower than those in the SCI group. The mitochondrial membrane potential and bcl-2 expression were up-regulated in the nimodipine-treated group. Taken together, our results indicate that the inhibition of calcium flux by nimodipine could reduce apoptosis processes and tissue damage through a mitochondrial pathway after spinal cord trauma [Current Zoology 57 （3）： 340-349, 2011].展开更多
Two isostructu ral Ni(Ⅱ)/Co(Ⅱ)-based metal-organic frameworks(MOFs),namely {[M_(3)(L)_(2)(bpb)_(3)(H_(2) O)_(4)]·2 DMF·2 H_(2)O}_(n) [M=Ni(HL-5,HL is short for Hui-Ling Liu);M=Co(HL-6);H_(3) L=2',6'...Two isostructu ral Ni(Ⅱ)/Co(Ⅱ)-based metal-organic frameworks(MOFs),namely {[M_(3)(L)_(2)(bpb)_(3)(H_(2) O)_(4)]·2 DMF·2 H_(2)O}_(n) [M=Ni(HL-5,HL is short for Hui-Ling Liu);M=Co(HL-6);H_(3) L=2',6'-dimethyl-[1,1'-biphenyl]-3,4',5-tricarboxylic acid;bpb=1,4-bis(pyrid-4-yl)benzene],have been hydrothermally synthesized and structu rally characterized.Both HL-5 and HL-6,which have the same three-interpenetrated3 D pillared-layer framework with sqc306 type topology,present good selective methyl orange(MO)adsorption over rhodamine B(RhB).Moreover,the catalytic CO_(2) cycloaddition properties with epoxides of the two MOFs have also been studied at ambient pressure and temperature.展开更多
The development of high-performance and cost-effective electrocatalysts towards oxygen reduction reaction(ORR) is of significant importance,but still challenging for the practical applications in related energy system...The development of high-performance and cost-effective electrocatalysts towards oxygen reduction reaction(ORR) is of significant importance,but still challenging for the practical applications in related energy systems.ORR process typically suffers from sluggish kinetics,the exploration of ORR electrocatalyst thus requires elaborate design.Herein,an effective strategy is developed for growing Co/N-doped carbon nanotube arrays on 2D MOFs-derived matrix via the pyrolysis of Co/Zn metalorganic-framework(MOF) nanosheets.The Co/Zn-MOF nanosheets serve as both the self-template for the 2D carbonized framework morphology and C/N source for the in-situ growth of 1D N-doped carbon nanotubes.The constructed hie rarchical architecture effectively integrates the OD/1D Co nanoparticle/Ndoped carbon nanotube interface and 1D(nanotubes)/2D(nanosheets) junction into frameworks with highly exposed active surface,enhanced mass-transport kinetics and electrical conductivity.As a result,the designed composite exhibits superior ORR activity and durability in alkaline media as compared to commercial Pt/C.Particularly,it shows promising ORR performance with a half-wave potential of 0.78 V versus reversible hydrogen electrode and negligible activity attenuation after 5000 potential cycles in acidic electrolyte.The designed strategy can be extended to construct other MOFs-derived carbon matrixes with diverse hierarchical structures and provide an efficient avenue for searching highperformance electrocatalysts.展开更多
Synthetic conditions and ligands are the key structural defining factors of metal-organic frameworks(MOFs).Therefore,reasonable optimization of these aspects is considered to be an effective means for designing materi...Synthetic conditions and ligands are the key structural defining factors of metal-organic frameworks(MOFs).Therefore,reasonable optimization of these aspects is considered to be an effective means for designing materials with novel structures and target functions.Herein,two novel Co(Ⅱ)-based MOFs,namely[Co(HL)(dibp)]_(n)(HL-8) and{[Co_(2)(L)(OH)(dibp)]·DMA}_n(HL-9)(H_(3)L=2',6'-dimethyl-[1,1'-biphenyl]-3,4',5-tricarboxylic acid;dibp=4,4'-di(1H-imidazol-1-yl)-1,1-biphenyl]),have been hydrothermally synthesized and structurally characterized.HL-8 crystallizes in the orthorhombic system (Pna2_(1)) with a grid layer structure,while HL-9 crystallizes in the monoclinic P2_(1)/n space group assembled through Co_(4)(OH)_(2)clusters with organic ligands.Remarkably,benefiting from the finite cage-like structure,HL-9 exhibited enhanced performance in carbon dioxide(CO_(2)) adsorption/catalytic transformation and excellent size selectivity during dye molecular adsorption process.展开更多
Pd-MnO2/TiO2 nanotube arrays（NTAs） photo-electrodes were successfully fabricated via anodization and electro deposition subsequently; the obtained Pd-MnO2/TiO2 NTAs photo electrodes were analyzed by scanning electro...Pd-MnO2/TiO2 nanotube arrays（NTAs） photo-electrodes were successfully fabricated via anodization and electro deposition subsequently; the obtained Pd-MnO2/TiO2 NTAs photo electrodes were analyzed by scanning electron microscopy（SEM）, X-ray diffraction（XRD） and characterized accordingly. Moreover, the light harvesting and absorption properties were investigated via ultraviolet–visible diffuse reflectance spectrum（DRS）; photo degradation efficiency was investigated via analyzing the photo catalytic degradation of Rhodamine B under visible illumination（xenon light）. The performed analyses illustrated that Pd-MnO2 codoped particles were successfully deposited onto the surface of the TiO2 nanotube arrays;DRS results showed significant improvement in visible light absorption which was between400 and 700 nm. Finally, the photo catalytic degradation efficiency results of the designated organic pollutant（Rhodamine B） illustrated a superior photocatalytic（PC） efficiency of approximately 95% compared to the bare TiO2 NTAs, which only exhibited a photo catalytic degradation efficiency of approximately 61%, thus it indicated the significant enhancement of the light absorption properties of fabricated photo electrodes and their yield of UOH radicals.展开更多
Inhaled drug-containing nanocarriers have been well applied as an important strategy in pulmonary dis-eases.Acute lung injury(ALI)is characterized by abnormal lung tension and complex pathogenesis,with high mortality ...Inhaled drug-containing nanocarriers have been well applied as an important strategy in pulmonary dis-eases.Acute lung injury(ALI)is characterized by abnormal lung tension and complex pathogenesis,with high mortality because of the limitations of targeted intervention.Accordingly,in the present study,we first identified that the mechanosensitive ion channel Piezo1 participated in the ALI-associated processes induced by lipopolysaccharide(LPS)in vitro and in vivo.Then,chitosan-stabilized bovine serum albu-min nanoparticles(NCs)emulsified with the Piezo1 inhibitor GsMTx4(NC-GsMTx4)were generated and exhibited excellent biocompatibility and biological function.Through aerosol inhalation,NC-GsMTx4 im-proved lung injury and inhibited cell apoptosis in LPS-stressed ALI mice and alleviated pulmonary fibrosis during the later stage of ALI.Mechanistically,GsMTx4 could regulate inflammation and apoptosis in lung epithelial cells via NF-κB and ERK1/2 signaling.In summary,our findings provide new insights into the pathological mechanisms of Piezo1 in ALI progression,and nebulized inhalation of NC-GsMTx4 offers a prospective platform for targeting Piezo1 to treat ALI efficiently and conveniently.展开更多
As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduate...As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.展开更多
Authors of papers to proceedings have to type these in a form suitable for direct photographic reproduction by the publisher. In order to ensure uniform style throughout the volume, all the papers have to be prepared ...Authors of papers to proceedings have to type these in a form suitable for direct photographic reproduction by the publisher. In order to ensure uniform style throughout the volume, all the papers have to be prepared strictly according to the instructions set below. A laser printer should be used to print the text. The publisher will reduce the camera-ready copy to 75% and print it in black only. For the convenience of the authors template files for MS Word 6.0 （and higher） are provided. In recent years, computer-assisted language learning （CALL） is widely used in College English teaching, it has played a positive role in improving the promotion of the quality of teaching. In this paper, itdiscussed CALL in terms of the specific application of curricular and extra-curricular.展开更多
Objective:To strengthen preoperative preparation management and reduce preoperative preparation defects.Methods:We set up a special medical care management team to investigate the preoperative preparation in surgical ...Objective:To strengthen preoperative preparation management and reduce preoperative preparation defects.Methods:We set up a special medical care management team to investigate the preoperative preparation in surgical departments,analyze the causes of the shortcomings in preoperative preparation,and propose countermeasures.The medical staff jointly formulated plans related to preoperative preparation management including clear outline of responsibilities,strengthening of training,integrated medical care,timely feedback,and continuous improvement.Besides,the preoperative preparation of patients before and after the improvement measures were compared using statistical analysis.Results:The rate of inadequate preoperative preparation was 11.14 per thousand before the integrated management model was implemented.After the implementation of the integrated management model,the rate of inadequate preoperative preparation decreased to 2.99 per thousand,and the difference was statistically(P<0.05).Conclusion:The integrated management of preoperative preparation can help reduce the rate of preoperative complications and ensure the safety of surgery.展开更多
基金supported by the National Natural Science Foundation of China Project(32200387)the Emergency Project for Risk Assessment of Areca Nut(Key Project of the Department of Agriculture and Rural Affairs of Hainan Province&Wanning Municipal People’s Government).
文摘Areca nut is the basic ingredient of betel quid,which is chewed by hundreds of millions of people in South-Eastern Asia.Chewing of areca nut has been associated with oral cancers potentially due to its specific alkaloids,among which arecoline constitutes about 90%of total fraction.Being the world’s fourth most commonly used psychoactive substance,arecoline evokes stimulation,addiction,and other direct neurological effects,while its misuse correlates to neurotoxic effects.However,what might underlie its neurotoxic mechanisms has been poorly documented.The brain is encoded by a complex network of neuronal and glial cell types,and neurotoxicity of hazardous compounds present transcriptional heterogeneity.Recently,the eusocial bumblebee has been used as a model for studying brain effects,with sophisticated cognitive capability and precisely measured brain architecture.Here,we decipher cell-type-specific mental risks to arecoline using bumblebees.Arecoline induced locomotor hyperactivity and cognitive impairment.Single nucleus RNA sequencing(snRNA-seq)unearthed arecoline-induced cell-specific responses,primarily targeted on Kenyon cells(KC).Moreover,high-dose arecoline induced distinctive cell responses among KC subtypes,particularly class I large Kenyon cell(lKC),leading to DNA damage,excitatory/inhibitory(E/I)imbalance,and calcium dyshomeostasis,which potentially resulted in cognitive impairment.Given arecoline’s popularity and growing exposure risks to humans,neurological health risks of areca nut warrant serious consideration.
基金supported by the National Natural Science Foundation of China(No.52170138)the National Key Research and Development Program of China(No.2023YFD1900305)+3 种基金Qingdao Agricultural University Doctoral Start-Up Fund(No.6631122003)the Project of Talent IntroductionEducation Program of Youth Innovation Teams in Universities of Shandong Province(2021–05)Shandong Provincial College Youth Innovation Team Program(No.2023KJ169).
文摘Cyclosporin A fermentation residue(CFR)is a type of organic waste generated during the production of cyclosporin A,which are abundant in nutrients including organic matter,phosphorus,nitrogen and potassium.Inappropriate handling of CFR not only waste valuable bioresources,but may also lead to the cyclosporin A and associated resistance genes into the natural environment,posing a significant threat to ecological system and human health.Land application was an effective way to resource recovery of CFR after aerobic composting(CAC).This study investigated the impact of CAC on soil fertility and environmental safety.The results indicated that CAC could improve soil nutrient contents and enhance enzyme activities.CAC altered the diversity and community composition of soil bacteria,resulting in an increase in the abundance of relevant bacteria beneficial for organic matter decomposition and cyclosporin A degradation.The introduced cyclosporin A(71.69μg/kg)completely degraded within 20 days due to soil biodegradation.The significantly increased abundance of intIl,mdr3,pgp,TSR and pmra in the soil cultivation early stage were restored to the soil background level within 90 days,indicating a reduced risk of antimicrobial resistance.The results demonstrated that reasonable land application of CAC could improve soil fertility without antimicrobial resistance risk,which is helpful for evaluating the resource utilization value and environmental risks of antibiotic fermentation residue after aerobic composting.
基金funded by the National Natural Science Foundation of China(32170495)the Emergency Project for Risk Assessment of Areca Nut(Key Project of Department of Agriculture and Rural Affairs of Hainan Province&Wanning Municipal People’s Government)。
文摘Due to the complex natures of dietary food components,it is difficult to elucidate how the compounds affect host health.Dietary food often selectively presents its mechanism of action on different cell types,and participates in the modulation of targeted cells and their microenvironments within organs.However,the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased influences.Single-cell RNA sequencing(sc RNA-seq)has emerged as an indispensable methodology to decompose tissues into different cell types for the demonstration of transcriptional profiles of individual cells.Sc RNA-seq applications has been summarized on three typical organs(brain,liver,kidney),and two representative immune-and tumor related health problems.The everincreasing role of sc RNA-seq in dietary food research with further improvement can provide sub-cellular information and the coupling between other cellular modalities.In this review,we propose utilizing sc RNAseq to more effectively capture the subtle and complex effects of food chemicals,and how they may lead to health problems at single-cell resolution.This novel technique will be valuable to elucidate the underlying mechanism of both the health benefits of food nutrients and the detrimental consequences food toxicants at the cellular level.
基金This work was supported by the Natural Science Foundation of Guangdong Province(No.2023A1515030284)the Key Realm Research and Development Program of Guangdong Province(No.2020B0202080001)+1 种基金the Guangdong Laboratory for Lingnan Modern Agriculture Project(No.NT2021010)the Science and Technology Planning Project of Guangdong Province(No.2021B1212040008).
文摘Tri(2-chloropropyl)phosphate(TCPP)was an emerging contaminant of global concern because of its frequent occurrence,potential toxic effects,and persistence in the environment.Microbial degradation might be an efficient and safe removal method,but limited information was available.In this study,Providencia rettgeri was isolated from contaminated sediment and showed it could use TCPP as unique phosphorus source to promote growth,and decompose 34.7%of TCPP(1 mg/L)within 5 days.The microbial inoculation and the initial concentration of TCPP could affect the biodegradation efficient.Further study results indicated that TCPP decomposition by Providencia rettgeri was mainly via phosphoester bond hydrolysis,evidenced by the production of bis(2-chloropropyl)phosphate(C_(6)H_(13)Cl_(2)PO_(4))and mono-chloropropyl phosphate(C_(3)H_(8)ClPO_(4)).Both intracellular and extracellular enzymes could degrade TCPP,but intracellular degradation was dominant in the later reaction stage,and the presence of Cu^(2+) ions had a promoting effect.These findings developed novel insights into the potential mechanism of TCPP microbial degradation.
基金supported by the National Natural Science Foundation of China(Nos.21902022,81903501,21601028)Qingchuang Science and Technology Plan of Shandong Province(No.2021KJ054)+1 种基金the Natural Science Foundation of Shandong Province(Nos.ZR2018LB018,ZR2019QB026,ZR2022QB058,ZR2020KB014)Scientific Research Foundation of Dezhou University(Nos.30101905,30102708,30102701).
文摘Metal-organic frameworks(MOFs)functionalized with open metal sites(OMSs)have received widespread attention in various applications due to their fascinating electronic properties and unique interactions with vip molecules.However,rational tailoring of the coordination environment of metal nodes dur-ing the synthesis of MOFs remains a great challenge due to their tendency of saturated coordination with linkers.Herein,we reported the construction of three new MOFs featuring unsaturated Cu(Ⅱ)sites,namely[Cu(HCOO)(pzta)]_(n)(HL-1),{[Cu(PTA)0.5(pzta)(H_(2)O)]·2H_(2)O}_(n)(HL-2)and[Cu(NA)0.5(pzta)]_(n)(HL-3)(Hpzta=3-pyrazinyl-1,2,4-triazole;PTA=terephthalic acid;NA=1,4-naphthalene dicarboxylic acid),based on the mixed-linker strategy via specific selection of versatile Hpzta ligand and carboxylate ligands.Re-markably,the obtained MOFs exhibited excellent activity and good recyclability for the catalytic reduction of nitroaromatics under mild conditions(25℃and 1 atm).In particular,the complete conversion of 4-nitrophenol(4-NP)took only 30 s on HL-2,reaching a record-high TOF value compared with previously reported metal catalysts.The combined experimental and theoretical studies on HL-2 revealed that the open Cu site with positive-charged nature could improve the adsorption and subsequent electron trans-port between the substrates,and was responsible for the outstanding performance.This work shined lights on the further enhancement of performance for MOFs through rational OMSs construction.
基金supported by the National Social Science Fund of China(Grant No.22BJY188)the National Natural Science Foundation of China(Grant Nos.71934001,71573159,41771568,71533004,71974001)+4 种基金the National Key Research and Development Program of China(Grant No.2016YFA0602500)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA23070400)the Natural Science Foundation of Shandong Province(Grant No.ZR2021MG011)the Social Science Foundation of Shandong Province(Grant Nos.21CJJJ09,2020-NDJJ-07)the Major Project of Humanities and Social Sciences Research of Shandong University(Grant No.19RWZD05).
文摘Policymakers and managers have increasingly adopted green bonds as a direct financing tool to address environmental degradation and climate change in emerging economies;however,the increasing green washing sentiments in the green bond market raise questions on whether green bonds can nudge polluting businesses to achieve green transformation.Therefore,this study joins the controversial debate by investigating the impact of green bond issuance on corporate environmental responsibilities and the potential impact mechanisms and economic consequences.Using the data of Chinese listed enterprises from 2011 to 2020 and the staggered issuance of green bonds as plausibly exogenous shocks,we determine that the enterprises in the experimental group that issued green bonds increased their environmental performance compared to their counterparts.Furthermore,this positive link is maintains after a series of robustness tests.Moreover,we identify that green bond issuance plausibly enhances environmental responsibility engagements through two governance channels,namely,internal management and external supervision.This beneficial effect appears more pronounced for subsamples of firms in low-polluting industries,without environmental subsidies and with higher managerial abilities.Furthermore,economic consequences indicate that the issuance of green bonds primarily motivates speculative shareholder benefits,as evidenced by short-term increases in stock yields but with limited impact on the short-run financial performance.Overall,these findings offer new evidence supporting that green financing tools could play a helpful hand toward environmental sustainability.
文摘Objective: To explore the correlation between muscle strength, muscle mass and bone mineral density (BMD) in Zhuang female population, body composition analysis and grip strength, and to analyze the possible influencing factors of BMD. Methods: 182 postmenopausal women were selected from Guangxi Province of China. Broadband ultrasound attenuation (BUA) was used to evaluate BMD. Grip dynamometer to assess muscle strength. Height, weight and muscle mass of each part were measured by body composition measuring instrument. Body mass index (BMI), skeletal muscle mass index (SMI) and limb skeletal muscle mass (SM) were calculated according to the measurement results. Results: BUA, grip strength and SMI in postmenopausal women of Zhuang nationality showed a decreasing trend with age (p p p r = 0.305, p Conclusion: With the increase of age, the decline rate of muscle strength of postmenopausal Zhuang women in Guangxi is slower than that of BMD and muscle mass. SM can better reflect the BMD level of the body than SMI, and the LSM is the main influencing factor of BMD.
基金supported by the Natural Science Foundation of Shandong Province(ZR2016BM31)the Science and Technology Foundation of Jinan City(201311035)~~
文摘Combinations of graphene(Gr)and carbon black(C)were employed as binary carbon supports to fabricate Pd‐based electrocatalysts via one‐pot co‐reduction with Pd2+.The electrocatalytic performance of the resulting Pd/Gr‐C catalysts during the electrooxidation of formic acid was assessed.A Pd/Gr0.3C0.7(Gr oxide:C=3:7,based on the precursor mass ratio)electrocatalyst exhibited better catalytic performance than both Pd/C and Pd/Gr catalysts.The current density generated by the Pd/Gr0.3C0.7catalyst was as high as102.14mA mgPd?1,a value that is approximately3times that obtained from the Pd/C(34.40mA mgPd?1)and2.6times that of the Pd/Gr material(38.50mA mgPd?1).The anodic peak potential of the Pd/Gr0.3C0.7was120mV more negative than that of the Pd/C and70mV more negative than that of the Pd/Gr.Scanning electron microscopy images indicated that the spherical C particles accumulated on the wrinkled graphene surfaces to form C cluster/Gr hybrids having three‐dimensional nanostructures.X‐ray photoelectron spectroscopy data confirmed the interaction between the Pd metal and the binary Gr‐C support.The Pd/Gr0.3C0.7also exhibited high stability,and so is a promising candidate for the fabrication of anodes for direct formic acid fuel cells.This work demonstrates a simple and cost‐effective method for improving the performance of Pd‐based electrocatalysts,which should have potential industrial applications.
基金the National Natural Science Foundation of China (Nos. 81622032 and 51672184)the Jiangsu Innovation and Entrepreneurship Program+1 种基金the Natural Science Research of Jiangsu Higher Education Institutions (No. 17KJA180011)the US Hermann Foundation and the National Science Foundation (award DMR-0805172) for financial supports
文摘Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast(OB, bone forming cells) responses was investigated. Nanocrystalline diamond(NCD) and submicron crystalline diamond(SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition(MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay(ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates(each containing 30–50 cells) on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.
文摘Abstract Spinal cord injury （SCI） remains an unsolved human health challenge. To alleviate the impairments of SCI, we studied the therapeutic effect of nimodipine （an L-type Ca^2＋ channel antagonist） on functional recovery from SCI using Nystrom＇s method in a mouse model. Eighty-four mice were divided into three groups： control group in which only vertebral plates were cut off without causing any spinal injuries; SCI; and SCI with nimodipine treatment. We assessed the histopathology, apoptosis detection, cell cycle, mitochondrial transmembrane potential, bcl-2/bax and caspase-3 levels of tissue 8 h, 1 d, 3 d and 4 d after trauma to evaluate rehabilitation. Behavioral performances were also assessed before and after nimodipine treatment. Results from inclined plane tests, motor score assessment and histological observations indicated that mice in the nimodipine-treated group rehabilitated better than those in the SCI group. The ratio of apoptosis, caspase-3 and bax expression in the nimodip- ine-treated group were significantly lower than those in the SCI group. The mitochondrial membrane potential and bcl-2 expression were up-regulated in the nimodipine-treated group. Taken together, our results indicate that the inhibition of calcium flux by nimodipine could reduce apoptosis processes and tissue damage through a mitochondrial pathway after spinal cord trauma [Current Zoology 57 （3）： 340-349, 2011].
基金financially supported by the National Natural Science Foundation of China(NSFC,Nos.21601028,21902022 and 21371028)the NSF of Shandong Province(Nos.ZR2019QB026,ZR2018LB018,ZR2016BM26 and ZR2016BL04)。
文摘Two isostructu ral Ni(Ⅱ)/Co(Ⅱ)-based metal-organic frameworks(MOFs),namely {[M_(3)(L)_(2)(bpb)_(3)(H_(2) O)_(4)]·2 DMF·2 H_(2)O}_(n) [M=Ni(HL-5,HL is short for Hui-Ling Liu);M=Co(HL-6);H_(3) L=2',6'-dimethyl-[1,1'-biphenyl]-3,4',5-tricarboxylic acid;bpb=1,4-bis(pyrid-4-yl)benzene],have been hydrothermally synthesized and structu rally characterized.Both HL-5 and HL-6,which have the same three-interpenetrated3 D pillared-layer framework with sqc306 type topology,present good selective methyl orange(MO)adsorption over rhodamine B(RhB).Moreover,the catalytic CO_(2) cycloaddition properties with epoxides of the two MOFs have also been studied at ambient pressure and temperature.
基金supported by the National Natural Science Foundation of China (NSFC,Nos.21701124 and 51702236)Tianjin Municipal Science and Technology Commission (Nos. 18TCQNJC71500 and 17JCZDJC38000)the National Key R&D Program of China (No.2017YFA0700104)。
文摘The development of high-performance and cost-effective electrocatalysts towards oxygen reduction reaction(ORR) is of significant importance,but still challenging for the practical applications in related energy systems.ORR process typically suffers from sluggish kinetics,the exploration of ORR electrocatalyst thus requires elaborate design.Herein,an effective strategy is developed for growing Co/N-doped carbon nanotube arrays on 2D MOFs-derived matrix via the pyrolysis of Co/Zn metalorganic-framework(MOF) nanosheets.The Co/Zn-MOF nanosheets serve as both the self-template for the 2D carbonized framework morphology and C/N source for the in-situ growth of 1D N-doped carbon nanotubes.The constructed hie rarchical architecture effectively integrates the OD/1D Co nanoparticle/Ndoped carbon nanotube interface and 1D(nanotubes)/2D(nanosheets) junction into frameworks with highly exposed active surface,enhanced mass-transport kinetics and electrical conductivity.As a result,the designed composite exhibits superior ORR activity and durability in alkaline media as compared to commercial Pt/C.Particularly,it shows promising ORR performance with a half-wave potential of 0.78 V versus reversible hydrogen electrode and negligible activity attenuation after 5000 potential cycles in acidic electrolyte.The designed strategy can be extended to construct other MOFs-derived carbon matrixes with diverse hierarchical structures and provide an efficient avenue for searching highperformance electrocatalysts.
基金supported by the National Natural Science Foundation of China(Nos.21902022,21601028,81903501 and 22061019)Qingchuang Science and Technology Plan of Shandong Province(No.2021KJ054)+1 种基金the Natural Science Foundation of Shandong Province(Nos.ZR2018LB018,ZR2019QB026 and ZR2020KB014)Scientific Research Foundation of Dezhou University(Nos.30101905,30102708 and 30102701)。
文摘Synthetic conditions and ligands are the key structural defining factors of metal-organic frameworks(MOFs).Therefore,reasonable optimization of these aspects is considered to be an effective means for designing materials with novel structures and target functions.Herein,two novel Co(Ⅱ)-based MOFs,namely[Co(HL)(dibp)]_(n)(HL-8) and{[Co_(2)(L)(OH)(dibp)]·DMA}_n(HL-9)(H_(3)L=2',6'-dimethyl-[1,1'-biphenyl]-3,4',5-tricarboxylic acid;dibp=4,4'-di(1H-imidazol-1-yl)-1,1-biphenyl]),have been hydrothermally synthesized and structurally characterized.HL-8 crystallizes in the orthorhombic system (Pna2_(1)) with a grid layer structure,while HL-9 crystallizes in the monoclinic P2_(1)/n space group assembled through Co_(4)(OH)_(2)clusters with organic ligands.Remarkably,benefiting from the finite cage-like structure,HL-9 exhibited enhanced performance in carbon dioxide(CO_(2)) adsorption/catalytic transformation and excellent size selectivity during dye molecular adsorption process.
基金supported by the National Natural Science Foundation of China (No. 51178138)the National Creative Research Groups of China (No. 51121062)the State Key Laboratory of Urban Water Resources and Environment (No. 2010DX03)
文摘Pd-MnO2/TiO2 nanotube arrays（NTAs） photo-electrodes were successfully fabricated via anodization and electro deposition subsequently; the obtained Pd-MnO2/TiO2 NTAs photo electrodes were analyzed by scanning electron microscopy（SEM）, X-ray diffraction（XRD） and characterized accordingly. Moreover, the light harvesting and absorption properties were investigated via ultraviolet–visible diffuse reflectance spectrum（DRS）; photo degradation efficiency was investigated via analyzing the photo catalytic degradation of Rhodamine B under visible illumination（xenon light）. The performed analyses illustrated that Pd-MnO2 codoped particles were successfully deposited onto the surface of the TiO2 nanotube arrays;DRS results showed significant improvement in visible light absorption which was between400 and 700 nm. Finally, the photo catalytic degradation efficiency results of the designated organic pollutant（Rhodamine B） illustrated a superior photocatalytic（PC） efficiency of approximately 95% compared to the bare TiO2 NTAs, which only exhibited a photo catalytic degradation efficiency of approximately 61%, thus it indicated the significant enhancement of the light absorption properties of fabricated photo electrodes and their yield of UOH radicals.
基金supported by the Science and Technology Plan of Jiangxi Provincial Health Commission(No.SKJP220201115)Live Science and Technology of Suzhou(No.SS201862)the Prior-ity Academic Program Development of Jiangsu Higher Education Institutions(PAPD).Translational Research Grant of NCRCH(No.2020WSB08).
文摘Inhaled drug-containing nanocarriers have been well applied as an important strategy in pulmonary dis-eases.Acute lung injury(ALI)is characterized by abnormal lung tension and complex pathogenesis,with high mortality because of the limitations of targeted intervention.Accordingly,in the present study,we first identified that the mechanosensitive ion channel Piezo1 participated in the ALI-associated processes induced by lipopolysaccharide(LPS)in vitro and in vivo.Then,chitosan-stabilized bovine serum albu-min nanoparticles(NCs)emulsified with the Piezo1 inhibitor GsMTx4(NC-GsMTx4)were generated and exhibited excellent biocompatibility and biological function.Through aerosol inhalation,NC-GsMTx4 im-proved lung injury and inhibited cell apoptosis in LPS-stressed ALI mice and alleviated pulmonary fibrosis during the later stage of ALI.Mechanistically,GsMTx4 could regulate inflammation and apoptosis in lung epithelial cells via NF-κB and ERK1/2 signaling.In summary,our findings provide new insights into the pathological mechanisms of Piezo1 in ALI progression,and nebulized inhalation of NC-GsMTx4 offers a prospective platform for targeting Piezo1 to treat ALI efficiently and conveniently.
基金Supported by Guangdong Higher Education Teaching Reform ProjectGuangdong Ocean University Education and Teaching Reform Project(XJG201806)Guangdong Ocean University Degree and Postgraduate Education Reform Research Project(201709)
文摘As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.
文摘Authors of papers to proceedings have to type these in a form suitable for direct photographic reproduction by the publisher. In order to ensure uniform style throughout the volume, all the papers have to be prepared strictly according to the instructions set below. A laser printer should be used to print the text. The publisher will reduce the camera-ready copy to 75% and print it in black only. For the convenience of the authors template files for MS Word 6.0 （and higher） are provided. In recent years, computer-assisted language learning （CALL） is widely used in College English teaching, it has played a positive role in improving the promotion of the quality of teaching. In this paper, itdiscussed CALL in terms of the specific application of curricular and extra-curricular.
文摘Objective:To strengthen preoperative preparation management and reduce preoperative preparation defects.Methods:We set up a special medical care management team to investigate the preoperative preparation in surgical departments,analyze the causes of the shortcomings in preoperative preparation,and propose countermeasures.The medical staff jointly formulated plans related to preoperative preparation management including clear outline of responsibilities,strengthening of training,integrated medical care,timely feedback,and continuous improvement.Besides,the preoperative preparation of patients before and after the improvement measures were compared using statistical analysis.Results:The rate of inadequate preoperative preparation was 11.14 per thousand before the integrated management model was implemented.After the implementation of the integrated management model,the rate of inadequate preoperative preparation decreased to 2.99 per thousand,and the difference was statistically(P<0.05).Conclusion:The integrated management of preoperative preparation can help reduce the rate of preoperative complications and ensure the safety of surgery.
