Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty acids.Yet,the effect of culture conditions on growth and fatty acid composition vary widely among different thra...Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty acids.Yet,the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains.Two different thraustochytrid strains,Schizochytrium sp.PKU#Mn4 and Thraustochytriidae sp.PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions.Although they exhibited similar fatty acid profiles,PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions.Relative DHA content of 48.5%and 49.2%(relative to total fatty acids),respectively,were achieved on glycerol under their optimal flask culture conditions.Maximum DHA yield(Yp/x)of 21.0%and 18.9%and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained,respectively,in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy.A 3.4-and 2.8-fold improvement of DHA production(g/L),respectively,was achieved in this study.Overall,our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.展开更多
基金This work was partially supported by National Key R&D Program of China(2016YFA0601400)National Science Foundation of China(31670044,91751115,and 31602185).
文摘Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty acids.Yet,the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains.Two different thraustochytrid strains,Schizochytrium sp.PKU#Mn4 and Thraustochytriidae sp.PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions.Although they exhibited similar fatty acid profiles,PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions.Relative DHA content of 48.5%and 49.2%(relative to total fatty acids),respectively,were achieved on glycerol under their optimal flask culture conditions.Maximum DHA yield(Yp/x)of 21.0%and 18.9%and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained,respectively,in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy.A 3.4-and 2.8-fold improvement of DHA production(g/L),respectively,was achieved in this study.Overall,our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.
