AIM: To investigate the therapeutic effect of umbilical cordblood transplantation (UCBT) on patients with severe viralhepatitis and on liver lesions in rats.METHODS: One hundred and fifty three patients with severevir...AIM: To investigate the therapeutic effect of umbilical cordblood transplantation (UCBT) on patients with severe viralhepatitis and on liver lesions in rats.METHODS: One hundred and fifty three patients with severeviral hepatitis were included in the study between April 1990and July 2002. The patients were treated with adult plasmatransfusion (control), UCBT, plasma exchange (PE) and UCBTcombined with PE (UCBT+PE) respectively. The therapeuticeffectiveness was evaluated by serial determinations of liverfunction, lipids and immune function in all patients beforeand after the treatment. The model of experimental hepaticfailure was constructed in SD rats by injecting carbontetrachloride. Then, the rats were given normal saline, adultplasma or neonate cord blood intraperitoneally. After detectionof liver function, the rats were killed and morphologicalchanges of the liver were microscopically observed.RESULTS: UCBT group and UCBT+PE group had muchbetter improvement in liver and immune functions thancontrol group and PE group. The patients in UCBT+PE grouphad the best clinical efficacy. UCBT was safe and had noside effects. The animal experiment showed significantimprovements in liver function and survival rate in neonatecord blood group as compared with adult plasma group.The histopathology of rat′s liver indicated that neonate cordblood application could decrease the liver injury and increasehepatocellular regeneration.CONCLUSION: UCBT demonstrated a good therapeuticeffect on severe viral hepatitis and no obvious side effects.Umbilical cord blood can attenuate the liver lesions andreproduce hepatocyte. The treatment of UCBT combinedwith PE was much better than that of single plasma exchange, thus UCBT can enhance the therapeutic effect of plasma exchange on severe viral hepatitis.展开更多
基金the National Natural Science Foundation of China, No.39870651
文摘AIM: To investigate the therapeutic effect of umbilical cordblood transplantation (UCBT) on patients with severe viralhepatitis and on liver lesions in rats.METHODS: One hundred and fifty three patients with severeviral hepatitis were included in the study between April 1990and July 2002. The patients were treated with adult plasmatransfusion (control), UCBT, plasma exchange (PE) and UCBTcombined with PE (UCBT+PE) respectively. The therapeuticeffectiveness was evaluated by serial determinations of liverfunction, lipids and immune function in all patients beforeand after the treatment. The model of experimental hepaticfailure was constructed in SD rats by injecting carbontetrachloride. Then, the rats were given normal saline, adultplasma or neonate cord blood intraperitoneally. After detectionof liver function, the rats were killed and morphologicalchanges of the liver were microscopically observed.RESULTS: UCBT group and UCBT+PE group had muchbetter improvement in liver and immune functions thancontrol group and PE group. The patients in UCBT+PE grouphad the best clinical efficacy. UCBT was safe and had noside effects. The animal experiment showed significantimprovements in liver function and survival rate in neonatecord blood group as compared with adult plasma group.The histopathology of rat′s liver indicated that neonate cordblood application could decrease the liver injury and increasehepatocellular regeneration.CONCLUSION: UCBT demonstrated a good therapeuticeffect on severe viral hepatitis and no obvious side effects.Umbilical cord blood can attenuate the liver lesions andreproduce hepatocyte. The treatment of UCBT combinedwith PE was much better than that of single plasma exchange, thus UCBT can enhance the therapeutic effect of plasma exchange on severe viral hepatitis.
