Objective:Treating peripheral nerve injury(PNI)presents a clinical challenge due to limited axon regeneration.Strychni Semen,a traditional Chinese medicine,is clinically used for numbness and hemiplegia.However,its ro...Objective:Treating peripheral nerve injury(PNI)presents a clinical challenge due to limited axon regeneration.Strychni Semen,a traditional Chinese medicine,is clinically used for numbness and hemiplegia.However,its role in promoting functional recovery after PNI and the related mechanisms have not yet been systematically studied.Methods:A mouse model of sciatic nerve crush(SNC)injury was established and the mice received drug treatment via intragastric gavage,followed by behavioral assessments(adhesive removal test,hot-plate test and Von Frey test).Transcriptomic analyses were performed to examine gene expression in the dorsal root ganglia(DRGs)from the third to the sixth lumbar vertebrae,so as to identify the significantly differentially expressed genes.Immunofluorescence staining was used to assess the expression levels of superior cervical ganglia neural-specific 10 protein(SCG10).The ultra-trace protein detection technique was used to evaluate changes in gene expression levels.Results:Strychni Semen and its active compounds(brucine and strychnine)improved functional recovery in mice following SNC injury.Transcriptomic data indicated that Strychni Semen and its active compounds initiated transcriptional reprogramming that impacted cellular morphology and extracellular matrix remodeling in DRGs after SNC,suggesting potential roles in promoting axon regeneration.Imaging data further confirmed that Strychni Semen and its active compounds facilitated axon regrowth in SNC-injured mice.By integrating protein–protein interaction predictions,ultra-trace protein detection,and molecular docking analysis,we identified myeloperoxidase as a potentially critical factor in the axon regenerative effects conferred by Strychni Semen and its active compounds.Conclusion:Strychni Semen and its active compounds enhance sensory function by promoting axonal regeneration after PNI.These findings establish a foundation for the future applications of Strychni Semen and highlight novel therapeutic strategies and drug targets for axon regeneration.展开更多
基金financially supported by the National Natural Science Foundation of China(No.82474420,No.82273903)。
文摘Objective:Treating peripheral nerve injury(PNI)presents a clinical challenge due to limited axon regeneration.Strychni Semen,a traditional Chinese medicine,is clinically used for numbness and hemiplegia.However,its role in promoting functional recovery after PNI and the related mechanisms have not yet been systematically studied.Methods:A mouse model of sciatic nerve crush(SNC)injury was established and the mice received drug treatment via intragastric gavage,followed by behavioral assessments(adhesive removal test,hot-plate test and Von Frey test).Transcriptomic analyses were performed to examine gene expression in the dorsal root ganglia(DRGs)from the third to the sixth lumbar vertebrae,so as to identify the significantly differentially expressed genes.Immunofluorescence staining was used to assess the expression levels of superior cervical ganglia neural-specific 10 protein(SCG10).The ultra-trace protein detection technique was used to evaluate changes in gene expression levels.Results:Strychni Semen and its active compounds(brucine and strychnine)improved functional recovery in mice following SNC injury.Transcriptomic data indicated that Strychni Semen and its active compounds initiated transcriptional reprogramming that impacted cellular morphology and extracellular matrix remodeling in DRGs after SNC,suggesting potential roles in promoting axon regeneration.Imaging data further confirmed that Strychni Semen and its active compounds facilitated axon regrowth in SNC-injured mice.By integrating protein–protein interaction predictions,ultra-trace protein detection,and molecular docking analysis,we identified myeloperoxidase as a potentially critical factor in the axon regenerative effects conferred by Strychni Semen and its active compounds.Conclusion:Strychni Semen and its active compounds enhance sensory function by promoting axonal regeneration after PNI.These findings establish a foundation for the future applications of Strychni Semen and highlight novel therapeutic strategies and drug targets for axon regeneration.
